Tuning the substrate selectivity of meta-cleavage product hydrolase by domain swapping.

abstract：:The production of infective juvenile stages (IJ) of the entomopathogenic nematode Steinernema carpocapsae in the presence of its symbiotic bacterium Xenorhabdus nematophilus was carried out in orbitally agitated bottles. Four complex culture media (M1-M4) were used, containing from 8% to 28% (by vol.) agave juice (agu...

journal_title：Applied microbiology and biotechnology

authors： Islas-López MA,Sanjuan-Galindo R,Rodríguez-Hernández AI,Chavarría-Hernández N

更新日期：2005-07-01 00:00:00

abstract：:The alphaproteobacterial Roseobacter clade (Rhodobacterales) is one of the most important global players in carbon and sulfur cycles of marine ecosystems. The remarkable metabolic versatility of this bacterial lineage provides access to diverse habitats and correlates with a multitude of extrachromosomal elements. Fou...

journal_title：Applied microbiology and biotechnology

authors： Petersen J,Frank O,Göker M,Pradella S

更新日期：2013-04-01 00:00:00

abstract：:The capability of lactic acid bacteria (LAB) to produce exopoly- and oligosaccharides was and is the subject of expanding research efforts. Due to their physicochemical properties and health-promoting potential, exopoly- and oligosaccharides from food-grade LAB can be used in the food and other industries and may have...

journal_title：Applied microbiology and biotechnology

authors： Korakli M,Vogel RF

更新日期：2006-08-01 00:00:00

abstract：:Cryparin is an abundant cell-wall-associated hydrophobin of Cryphonectria parasitica. Although cryparin is encoded as a single copy gene, it is the most abundant protein produced by this fungus when grown in liquid culture. Studies to characterize the transcriptional regulatory element(s) found that the fragment betwe...

journal_title：Applied microbiology and biotechnology

authors： Kwon BR,Kim MJ,Park JA,Chung HJ,Kim JM,Park SM,Yun SH,Yang MS,Kim DH

更新日期：2009-05-01 00:00:00

abstract：:The recent use of photosynthetic organisms such as Chlamydomonas reinhardtii in biomedical applications has demonstrated their potential for the treatment of acute and chronic tissue hypoxia. Moreover, transgenic microalgae have been suggested as an alternative in situ drug delivery system. In this study, we set out t...

journal_title：Applied microbiology and biotechnology

authors： Jarquín-Cordero M,Chávez MN,Centeno-Cerdas C,Bohne AV,Hopfner U,Machens HG,Egaña JT,Nickelsen J

更新日期：2020-01-01 00:00:00

abstract：:Previous studies have shown that a 20-kDa protein enhances production of the insecticidal CytA and CryIVA proteins of Bacillus thuringiensis in Escherichia coli as well as CytA production and crystal formation in B. thuringiensis. To determine whether the 20-kDa protein could enhance CryIVD production, an expression v...

journal_title：Applied microbiology and biotechnology

authors： Wu D,Federici BA

更新日期：1995-01-01 00:00:00

abstract：:Drug-resistant bacteria are becoming an increasingly widespread problem in the clinical setting. The current pipeline of antibiotics cannot provide satisfactory options for clinicians, which brought increasing attention to the development and application of non-traditional antimicrobial substances as alternatives. Met...

journal_title：Applied microbiology and biotechnology

authors： Ye Q,Chen W,Huang H,Tang Y,Wang W,Meng F,Wang H,Zheng Y

更新日期：2020-06-01 00:00:00

abstract：:Bacillus stearothermophilus, a thermophilic bacterium isolated from the Kuwaiti desert, produced a variety of monohydroxy androstene derivatives and an oxidized product when incubated with exogenous testosterone for 24 h at 65 degrees C. The major metabolite was identified as androst-4-en-3,17-dione while minor metabo...

journal_title：Applied microbiology and biotechnology

authors： Al-Awadi S,Afzal M,Oommen S

更新日期：2003-07-01 00:00:00

abstract：:Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A s...

journal_title：Applied microbiology and biotechnology

authors： Morikawa M,Daido H,Pongpobpibool S,Imanaka T

更新日期：1994-11-01 00:00:00

abstract：:Hemidesmus indicus (L.) R. Br. ex Schult., commonly known as Indian sarsaparilla or Anantamul, is an ethnopharmacologically important medicinal plant from the family Apocynaceae. Biosynthesis of an array of bioactive compounds such as methoxybenzaldehydes and their derivatives has been accountable for its intensive me...

journal_title：Applied microbiology and biotechnology

authors： Nandy S,Singh J,Pandey DK,Dey A

更新日期：2020-10-01 00:00:00

abstract：:Recently, it was shown that electrical DNA-chips in connection with a magnetic bead-based sandwich-hybridization assay can be a suitable alternative for the analysis of gene expression by monitoring the respective mRNA levels. In this study, we established an improved assay which allowed for a significantly shortened ...

journal_title：Applied microbiology and biotechnology

authors： Pioch D,Jürgen B,Evers S,Maurer KH,Hecker M,Schweder T

更新日期：2008-03-01 00:00:00

abstract：:A cephalosporin deacetylating acetyl xylan esterase was cloned from the genomic DNA of Bacillus subtilis CICC 20034 and functionally expressed in Escherichia coli. Its gene contained an open reading frame of 957 bp encoding 318 amino acids with a calculated mass of 35,607 Da, and it displayed significant identity to a...

journal_title：Applied microbiology and biotechnology

authors： Tian Q,Song P,Jiang L,Li S,Huang H

更新日期：2014-03-01 00:00:00

abstract：:The effects of Bacillus sp. SLS18, a plant-growth-promoting endophyte, on the biomass production and Mn/Cd uptake of sweet sorghum (Sorghum bicolor L.), Phytolacca acinosa Roxb., and Solanum nigrum L. were investigated. SLS18 displayed multiple heavy metals and antibiotics resistances. The strain also exhibited the ca...

journal_title：Applied microbiology and biotechnology

authors： Luo S,Xu T,Chen L,Chen J,Rao C,Xiao X,Wan Y,Zeng G,Long F,Liu C,Liu Y

更新日期：2012-02-01 00:00:00

abstract：:This study represents two different large-scale proteomic experiments analyzing the antibiotic response and the mechanisms of production of β-lactamases in the nosocomial pathogen Stenotrophomonas maltophilia. Two-dimensional gel electrophoresis on the cytoplasmic protein fraction, together with iTRAQ® differential la...

journal_title：Applied microbiology and biotechnology

authors： Van Oudenhove L,De Vriendt K,Van Beeumen J,Mercuri PS,Devreese B

更新日期：2012-08-01 00:00:00

abstract：:Yarrowia lipolytica is an industrial yeast that has been used in the sustainable production of fatty acid-derived and lipid compounds due to its high growth capacity, genetic tractability, and oleaginous properties. This investigation examines the possibility of utilizing urea or urine as an alternative to ammonium su...

journal_title：Applied microbiology and biotechnology

authors： Brabender M,Hussain MS,Rodriguez G,Blenner MA

更新日期：2018-03-01 00:00:00

abstract：:Immunostimulatory glucose polymers known as beta-glucans have been studied for many years. Our laboratory has prepared and characterized a novel microparticulate beta-glucan (MG) from the budding yeast Saccharomyces cerevisiae. Because MG particles are rapidly phagocytized by murine peritoneal macrophages and induce t...

journal_title：Applied microbiology and biotechnology

authors： Berner VK,Sura ME,Hunter KW Jr

更新日期：2008-10-01 00:00:00

abstract：:Comparative metabolic responses of Sinorhizobium sp. C4 were investigated. Comprehensive metabolites profiles, including polar metabolites, fatty acids, and polyhydroxyalkanoates were evaluated through untargeted metabolome analyses. Intracellular metabolomes during the degradation of phenanthrene were compared with t...

journal_title：Applied microbiology and biotechnology

authors： Keum YS,Seo JS,Li QX,Kim JH

更新日期：2008-10-01 00:00:00

abstract：:Optimization of yields and productivities in reductive whole-cell biotransformations is an important issue for the industrial application of such processes. In a recent study with Escherichia coli, we analyzed the reduction of the prochiral β-ketoester methyl acetoacetate by an R-specific alcohol dehydrogenase (ADH) t...

journal_title：Applied microbiology and biotechnology

authors： Siedler S,Bringer S,Blank LM,Bott M

更新日期：2012-02-01 00:00:00

abstract：:Multidrug-resistant Acinetobacter baumannii is a well-documented pathogen associated with hospital-acquired infections. In addition to multidrug resistance, A. baumannii can also become resistant to colistin, the antibiotic treatment of last resort, by the loss of the lipopolysaccharide from its outer membrane. Here, ...

journal_title：Applied microbiology and biotechnology

authors： Tseng CC,Tsai YH,Hu A,Liou JW,Chang KC,Chang HH

更新日期：2016-10-01 00:00:00

abstract：:Cytophaga hutchinsonii is a Gram-negative gliding bacterium, which can rapidly degrade crystalline cellulose via a novel strategy without any recognizable processive cellulases. Its mechanism of cellulose binding and degradation is still a mystery. In this study, the mutagenesis of C. hutchinsonii with the mariner-bas...

journal_title：Applied microbiology and biotechnology

authors： Ji X,Xu Y,Zhang C,Chen N,Lu X

更新日期：2012-10-01 00:00:00

abstract：:Biocatalyzed synthesis of nucleoside analogues was carried out using two thermostable nucleoside phosphorylases from the hyperthermophilic aerobic crenarchaeon Aeropyrum pernix K1. The synthesis of the 2,6-diaminopurine nucleoside and 5-methyluridine was used as a reaction model to test the process. Both the purine nu...

journal_title：Applied microbiology and biotechnology

authors： Zhu S,Song D,Gong C,Tang P,Li X,Wang J,Zheng G

更新日期：2013-08-01 00:00:00

abstract：:Bacterial polyhedral organelles are extremely large macromolecular complexes consisting of metabolic enzymes encased within a multiprotein shell that is somewhat reminiscent of a viral capsid. Recent investigations suggest that polyhedral organelles are widely used by bacteria for optimizing metabolic processes. The d...

journal_title：Applied microbiology and biotechnology

authors： Bobik TA

更新日期：2006-05-01 00:00:00

abstract：:The nitrate-dependent denitrifying anaerobic methane oxidation (DAMO) process, which is metabolized together by anaerobic methanotrophic archaea and NC10 phylum bacteria, is expected to be important for the global carbon and nitrogen cycles. However, there are little studies about the existence of this process and the...

journal_title：Applied microbiology and biotechnology

authors： Ding J,Fu L,Ding ZW,Lu YZ,Cheng SH,Zeng RJ

更新日期：2016-01-01 00:00:00

abstract：:Twenty-three genotypes of maitake (Grifola frondosa) from Asia, North America and Europe were compared for biological efficiency (BE), quality and crop cycle time. Significant differences among lines were found for BE and crop cycle time when mushrooms were produced on nutrient-supplemented (15% millet, 10% wheat bran...

journal_title：Applied microbiology and biotechnology

authors： Shen Q,Royse DJ

更新日期：2002-02-01 00:00:00

abstract：:Significant decreases in the apparent viscosity of a bacterial biofilm suspension were measured following addition of sodium, potassium, magnesium, or calcium salts, whereas iron salts increased the viscosity. Electrostatic interactions contribute to biofilm cohesion and iron cations are potent crosslinkers of the bio...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Stewart PS

更新日期：2002-09-01 00:00:00

abstract：:α-L-Rhamnosyl-β-D-glucosidase (rutinosidase) hydrolyzes the glycosidic linkage between the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose) and the aglycone. We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergi...

journal_title：Applied microbiology and biotechnology

authors： Ishikawa M,Kawasaki M,Shiono Y,Koseki T

更新日期：2018-04-01 00:00:00

abstract：:An on-line technique, based on measuring the increase in pressure due to CO(2) release in a closed air-tight reactor, was used to evaluate the fermentation of lactate by propionibacteria. The method was applied to batch cultures of Propionibacterium shermanii grown in yeast extract/sodium lactate medium containing lac...

journal_title：Applied microbiology and biotechnology

authors： Benjelloun H,Rochex A,Lecouturier D,Dechemi S,Lebeault JM

更新日期：2005-08-01 00:00:00

abstract：:A single-stage continuous fermentation process for the production of 2-keto-L-gulonic acid (2KGA) from L-sorbose using Ketogulonigenium vulgare DSM 4025 was developed. The chemostat culture with the dilution rate that was calculated based on the relationship between the 2KGA production rate and the 2KGA concentration ...

journal_title：Applied microbiology and biotechnology

authors： Takagi Y,Sugisawa T,Hoshino T

更新日期：2009-04-01 00:00:00

abstract：:Poly(lactate-co-3-hydroxybutyrate) (P(LA-co-3HB)) was previously produced from xylose in engineered Escherichia coli. The aim of this study was to increase the polymer productivity and LA fraction in P(LA-co-3HB) using two metabolic engineering approaches: (1) deletions of competing pathways to lactate production and ...

journal_title：Applied microbiology and biotechnology

authors： Nduko JM,Matsumoto K,Ooi T,Taguchi S

更新日期：2014-03-01 00:00:00

abstract：:Biodegradation of petroleum compounds in saline environments seems intricate and needs more attention. In this study, tetracosane was used to enrich alkane-degrading bacteria from oil-contaminated saline soils. Among the isolates, strain Qtet3, with the highest 16s rRNA gene sequence similarity to Alcanivorax dieselol...

journal_title：Applied microbiology and biotechnology

authors： Dastgheib SM,Amoozegar MA,Khajeh K,Ventosa A

更新日期：2011-04-01 00:00:00