Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch culture on ethanol.

abstract：:The bacterial phosphoenolpyruvate (PEP)-dependent group translocation system (PTS) requires the presence of both membrane-bound and cytoplasmic components to phosphorylate and translocate sugar. Deinococcus radiodurans has a functional fruA gene coding for the membrane-bound components of the fructose-specific PTS. Ho...

journal_title：Applied microbiology and biotechnology

authors： Lee HY,Magotra M,Wong TY,Chakraborty C,Liu JK

更新日期：2012-02-01 00:00:00

abstract：:The nitrile hydratase (NHase, EC 4.2.1.84) genes (alpha and beta subunit) and the corresponding activator gene from Rhodococcus equi TG328-2 were cloned and sequenced. This Fe-type NHase consists of 209 amino acids (alpha subunit, M(r) 23 kDa) and 218 amino acids (beta subunit, M(r) 24 kDa) and the NHase activator of ...

journal_title：Applied microbiology and biotechnology

authors： Rzeznicka K,Schätzle S,Böttcher D,Klein J,Bornscheuer UT

更新日期：2010-02-01 00:00:00

abstract：:Synthetic biology aims at manipulating biological systems by rationally designed and genetically introduced components. Efforts in photoactuator engineering resulted in microorganisms reacting to extracellular light-cues with various cellular responses. Some of them lead to the formation of macroscopically observable ...

journal_title：Applied microbiology and biotechnology

authors： Renicke C,Taxis C

更新日期：2016-04-01 00:00:00

abstract：:The gene ce1A, encoding an endoglucanase from the strain Bacillus sp. BP-23, was cloned and expressed in Escherichia coli. The nucleotide sequence of a 1867-bp DNA fragment containing the ce1A gene was determined, revealing an open reading frame of 1200 nucleotides that encodes a protein of 44,803 Da. The deduced amin...

journal_title：Applied microbiology and biotechnology

authors： Blanco A,Díaz P,Martínez J,Vidal T,Torres AL,Pastor FI

更新日期：1998-07-01 00:00:00

abstract：:Chemical mutagenesis of the phototrophic green microalga Parietochloris incisa, producing high amounts of arachidonic acid (ARA), resulted in selection of a mutant deficient in ARA and rich in dihomo-γ-linolenic acid (DGLA) and thus ∆5 desaturase defective. The mutagenesis produced a nonsense mutation in the ∆5 desatu...

journal_title：Applied microbiology and biotechnology

authors： Iskandarov U,Khozin-Goldberg I,Cohen Z

更新日期：2011-04-01 00:00:00

abstract：:In bioelectrochemical systems, exoelectrogenic bacteria respire with anode electrodes as their extracellular electron acceptor; therefore, lower anode potentials can reduce the energy gain to each microbe and select against ones that are not able to respire at a lower potential range. Often fully developed anode commu...

journal_title：Applied microbiology and biotechnology

authors： Yan H,Yates MD,Regan JM

更新日期：2015-11-01 00:00:00

abstract：:Photothermal cancer therapy, as a prospective approach for local cancer treatment, is attracting increasing interests. In this paper, gold nanorods were conjugated with folate (folate/AuNRs), and their photothermal effects on hepatocellular carcinoma cell line (HepG2) using MTT assay, flow cytometry, as well as on the...

journal_title：Applied microbiology and biotechnology

authors： Jin H,Yang P,Cai J,Wang J,Liu M

更新日期：2012-06-01 00:00:00

abstract：:Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in physiology, ecology, cell biology, and genetics. However, it is unclear whether th...

journal_title：Applied microbiology and biotechnology

authors： Zhang N,Fan Y,Li C,Wang Q,Leksawasdi N,Li F,Wang S

更新日期：2018-05-01 00:00:00

abstract：:Clavulanic acid is a secondary metabolite produced by Streptomyces clavuligerus. It possesses a clavam structure and a characteristic 3R,5R stereochemistry essential for action as a beta-lactamase inhibitory molecule. It is produced from glyceraldehyde-3-phosphate and arginine in an eight step biosynthetic pathway. Th...

journal_title：Applied microbiology and biotechnology

authors： Liras P,Rodríguez-García A

更新日期：2000-10-01 00:00:00

abstract：:Knowledge about methanotrophs and their activities is important to understand the microbial mediation of the greenhouse gas CH(4) under climate change and human activities in terrestrial ecosystems. The effects of simulated warming and sheep grazing on methanotrophic abundance, community composition, and activity were...

journal_title：Applied microbiology and biotechnology

authors： Zheng Y,Yang W,Sun X,Wang SP,Rui YC,Luo CY,Guo LD

更新日期：2012-03-01 00:00:00

abstract：:Polygalacturonases (PG) have evolved in the past years from a pectinase "simply" being used for food processing to an important parameter in plant-fungal interaction. PG-inhibiting proteins (PGIP) that are synthesised in plants as a specific response to PGs of pathogenic fungi, have become a focus as a possible target...

journal_title：Applied microbiology and biotechnology

authors： Lang C,Dörnenburg H

更新日期：2000-04-01 00:00:00

abstract：:A precise and scarless genome excision method, employing the Cre/loxP system in concert with double-strand break (DSB)-stimulated intramolecular recombination was developed. The DSBs were mediated by the restriction endonuclease, I-SceI. It permitted multiple deletions of independent 14-, 43-, and 10-kb-long genomic r...

journal_title：Applied microbiology and biotechnology

authors： Suzuki N,Nonaka H,Tsuge Y,Okayama S,Inui M,Yukawa H

更新日期：2005-11-01 00:00:00

abstract：:Lipophilic extractives in wood and other lignocellulosic materials exert a negative impact in pulp and paper manufacturing causing the so-called pitch problems. In this work, the appropriateness of an enzymatic treatment using the laccase-mediator system for pitch biocontrol is evaluated. With this purpose, three pulp...

journal_title：Applied microbiology and biotechnology

authors： Gutiérrez A,del Río JC,Rencoret J,Ibarra D,Martínez AT

更新日期：2006-10-01 00:00:00

abstract：:The present review describes the possibility of in vitro meat production with the help of advanced co-culturing methods. In vitro meat production method could be a possible alternative for the conventional meat production. Originally, the research on in vitro meat production was initiated by the National Aeronautics a...

journal_title：Applied microbiology and biotechnology

authors： Pandurangan M,Kim DH

更新日期：2015-07-01 00:00:00

abstract：:The production of biogas by anaerobic digestion (AD) of agricultural residues, organic wastes, animal excrements, municipal sludge, and energy crops has a firm place in sustainable energy production and bio-economy strategies. Focusing on the microbial community involved in biomass conversion offers the opportunity to...

journal_title：Applied microbiology and biotechnology

authors： Hassa J,Maus I,Off S,Pühler A,Scherer P,Klocke M,Schlüter A

更新日期：2018-06-01 00:00:00

abstract：:This study represents two different large-scale proteomic experiments analyzing the antibiotic response and the mechanisms of production of β-lactamases in the nosocomial pathogen Stenotrophomonas maltophilia. Two-dimensional gel electrophoresis on the cytoplasmic protein fraction, together with iTRAQ® differential la...

journal_title：Applied microbiology and biotechnology

authors： Van Oudenhove L,De Vriendt K,Van Beeumen J,Mercuri PS,Devreese B

更新日期：2012-08-01 00:00:00

abstract：:Trichothecenes are toxic secondary metabolites produced by filamentous fungi mainly belonging to the Fusarium genus. Production of these mycotoxins occurs during infection of crops and is a threat to human and animal health. Although the pathway for biosynthesis of trichothecenes is well established, the regulation of...

journal_title：Applied microbiology and biotechnology

authors： Merhej J,Richard-Forget F,Barreau C

更新日期：2011-08-01 00:00:00

abstract：:Microbial transglutaminase (mTGase) is commonly known in the food industry as meat glue due to its incredible ability to "glue" meat proteins together. Aside from being widely exploited in the meat processing industries, mTGase is also widely applied in other food and textile industries by catalysing the formation of ...

journal_title：Applied microbiology and biotechnology

authors： Chan SK,Lim TS

更新日期：2019-04-01 00:00:00

abstract：:A two-stage two-stream chemostat system and a two-stage two-stream immobilized upflow packed-bed reactor system were used for the study of lactic acid production by Lactobacillus casei subsp casei. A mixing ratio of D12/D2 = 0.5 (D = dilution rate) resulted in optimum production, making it possible to generate continu...

journal_title：Applied microbiology and biotechnology

authors： Bruno-Bárcena JM,Ragout AL,Córdoba PR,Siñeriz F

更新日期：1999-03-01 00:00:00

abstract：:The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate (0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen ca...

journal_title：Applied microbiology and biotechnology

authors： Mukherjee J,Majumdar S,Scheper T

更新日期：2000-02-01 00:00:00

abstract：:The human antimicrobial peptide LL-37 is a cationic peptide with antimicrobial activity against both Gram-positive and Gram-negative microorganisms. This work describes the development of an expression system based on Escherichia coli capable of high production of the recombinant LL-37. The fusion protein Trx-LL-37 wa...

journal_title：Applied microbiology and biotechnology

authors： Krahulec J,Hyrsová M,Pepeliaev S,Jílková J,Cerný Z,Machálková J

更新日期：2010-09-01 00:00:00

abstract：:Microbial aggregates of an aerobic granular sludge can be used for the treatment of industrial or municipal wastewater, but their formation from a microbial activated sludge requires several weeks. Therefore, the aim of this research was the selection of microbial cultures to shorten the granule-forming period from se...

journal_title：Applied microbiology and biotechnology

authors： Ivanov V,Wang XH,Tay ST,Tay JH

更新日期：2006-04-01 00:00:00

abstract：:Lethal effects of pulsed electric fields (PEF) on suspensions of various bacteria, yeast, and spores in buffer solutions and liquid foodstuffs were examined. Living-cell counts of vegetative cell types were reduced by PEF treatment by up to more than four orders of magnitude (> 99.99%). On the other hand, endo- and as...

journal_title：Applied microbiology and biotechnology

authors： Grahl T,Märkl H

更新日期：1996-03-01 00:00:00

abstract：:The production of human gamma interferon as intracellular inclusion bodies in Escherichia coli, which simplified the purification process, is described. An expression plasmid carrying lipoprotein and the tryptophane promoters in tandem was used. Preparation of highly pure interferon was achieved using high resolution ...

journal_title：Applied microbiology and biotechnology

authors： Perez L,Vega J,Chuay C,Menendez A,Ubieta R,Montero M,Padron G,Silva A,Santizo C,Besada V

更新日期：1990-07-01 00:00:00

abstract：:Fungi can grow in extreme habitats, such as natural stone and mineral building materials, sometimes causing deterioration. Efflorescence-concentrated salt deposits-results from water movement through building material; it can damage masonry materials and other bricks. Fungal isolate KUR1, capable of growth on, and dis...

journal_title：Applied microbiology and biotechnology

authors： Masaphy S,Lavi I,Sultz S,Zabari L

更新日期：2014-06-01 00:00:00

abstract：:The protrusion (P) domain of the major structural protein VP1 of norovirus (NoV) is critical for the host's immune response and receptor binding. Most heterologous P domains expressed in Escherichia coli or Komagataella pastoris (formally known as Pichia pastoris) form P particles consisting of 24 P monomers formed th...

journal_title：Applied microbiology and biotechnology

authors： Chen YL,Chang PJ,Huang CT

更新日期：2018-11-01 00:00:00

abstract：:With advances in the development of DNA vaccines and gene therapy, there is a growing need for plasmid DNA with high quality for fundamental research and clinical trials. In this report, a scalable automated process for large-scale preparation of plasmid is described. This process is based on alkaline lysis and can be...

journal_title：Applied microbiology and biotechnology

authors： Li X,Jin H,Wu Z,Rayner S,Yin J,Yu Y,Zhang W,He Z,Wang C,Wang B

更新日期：2007-07-01 00:00:00

abstract：:To develop microbial production method for prenyl alcohols (e.g., (E,E)-farnesol (FOH), (E)-nerolidol (NOH), and (E,E,E)-geranylgeraniol (GGOH)), the genes encoding enzymes in the mevalonate and prenyl diphosphate pathways were overexpressed in Saccharomyces cerevisiae, and the resultant transformants were evaluated a...

journal_title：Applied microbiology and biotechnology

authors： Ohto C,Muramatsu M,Obata S,Sakuradani E,Shimizu S

更新日期：2009-04-01 00:00:00

abstract：:The transfer of plasmids was studied in a stirred fermentor in the course of mixed batch cultures combining recombinant strains of Lactococcus lactis subsp. lactis (donor strains) with L. lactis subsp. lactis CNRZ 268M3 (recipient strain). Donor strains contained one or two of the following plasmids (coding for erythr...

journal_title：Applied microbiology and biotechnology

authors： el Alami N,Boquien CY,Corrieu G

更新日期：1992-06-01 00:00:00

abstract：:Previous work showed that random mutagenesis produced a mutant of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 containing the V106A substitution in the hydroxylase alpha-subunit (TomA3) that changed the color of the cell suspension from wild-type brown to green in rich medium. Here, DNA shuffling was u...

journal_title：Applied microbiology and biotechnology

authors： Rui L,Reardon KF,Wood TK

更新日期：2005-01-01 00:00:00