Abstract：
:A precise and scarless genome excision method, employing the Cre/loxP system in concert with double-strand break (DSB)-stimulated intramolecular recombination was developed. The DSBs were mediated by the restriction endonuclease, I-SceI. It permitted multiple deletions of independent 14-, 43-, and 10-kb-long genomic regions on the Corynebacterium glutamicum genome. Accuracy of deletion was confirmed by the loss of marker genes, PCR, and sequencing of new genome joints. Eleven, 58, and 4 genes were predicted on the 14-, 43-, and 10-kb deleted regions, respectively. Although the resultant mutant lost a total of 67 kb encoding 73 genes, it still exhibited normal growth under standard laboratory conditions. Such a large segment deletion method in which multiple, successive deletions are possible is useful for genome engineering.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Suzuki N,Nonaka H,Tsuge Y,Okayama S,Inui M,Yukawa Hdoi
10.1007/s00253-005-1976-4subject
Has Abstractpub_date
2005-11-01 00:00:00pages
151-61issue
2eissn
0175-7598issn
1432-0614journal_volume
69pub_type
杂志文章abstract：:Purines are a primary source of carbon and nitrogen in soil; however, their metabolism is poorly understood in Streptomyces. Using a combination of proteomics, metabolomics, and metabolic engineering, we characterized the allantoin pathway in Streptomyces coelicolor. When cells grew in glucose minimal medium with alla...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-013-5372-1
更新日期：2014-01-01 00:00:00
abstract：:The alphaproteobacterial Roseobacter clade (Rhodobacterales) is one of the most important global players in carbon and sulfur cycles of marine ecosystems. The remarkable metabolic versatility of this bacterial lineage provides access to diverse habitats and correlates with a multitude of extrachromosomal elements. Fou...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-013-4746-8
更新日期：2013-04-01 00:00:00
abstract：:Glucose 6-phosphate dehydrogenase (G6PDH) is one of the most important dehydrogenases responsible for generating reduced NADPH for anabolism and is also the rate-limiting enzyme in the Entner-Doudoroff pathway. For in vitro biocatalysis, G6PDH must possess both high activity and good thermostability due to requirement...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-018-8798-7
更新日期：2018-04-01 00:00:00
abstract：:The xylose-fermenting yeast Spathaspora passalidarum showed excellent fermentation performance utilizing glucose and xylose under anaerobic conditions. But this yeast is highly sensitive to the inhibitors such as furfural present in the pretreated lignocellulosic biomass. In order to improve the inhibitor tolerance of...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3693-5
更新日期：2012-03-01 00:00:00
abstract：:Catharanthus roseus (L.) G. Don is a plant species known for its production of a variety of terpenoid indole alkaloids, many of which have pharmacological activities. Production of catharanthine in cell cultures or in hairy roots established by transformation with Agrobacterium rhizogenes is of interest because cathar...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3288-1
更新日期：2011-08-01 00:00:00
abstract：:The production of infective juvenile stages (IJ) of the entomopathogenic nematode Steinernema carpocapsae in the presence of its symbiotic bacterium Xenorhabdus nematophilus was carried out in orbitally agitated bottles. Four complex culture media (M1-M4) were used, containing from 8% to 28% (by vol.) agave juice (agu...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-004-1818-9
更新日期：2005-07-01 00:00:00
abstract：:Preparative-scale fermentation of gallic acid (3,4,5-trihydroxybenzoic acid) (1) with Beauveria sulfurescens ATCC 7159 gave two new glucosidated compounds, 4-(3,4-dihydroxy-6-hydroxymethyl-5-methoxy-tetrahydro-pyran-2-yloxy)-3-hydroxy-5-methoxy-benzoic acid (4), 3-hydroxy-4,5-dimethoxy-benzoic acid 3,4-dihydroxy-6-hyd...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-006-0692-z
更新日期：2007-03-01 00:00:00
abstract：:A newly isolated Aspergillus niger strain containing epoxide hydrolase was used to resolve racemic glycidyl azide and four derivatives to the (R)-enantiomers. After optimization of the biotransformation conditions, (R)-glycidyl azide was produced with good enantioselectivity (e.e.s > 95 %, E > 20). The substrate struc...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-012-4382-8
更新日期：2013-03-01 00:00:00
abstract：:Gene amplification using dihydrofolate reductase gene (dhfr) and methotrexate (MTX) is widely used for recombinant protein production in mammalian cells and is typically conducted in DHFR-deficient Chinese hamster ovary (CHO) cell lines. Generation of DHFR-deficient cells can be achieved by an expression vector incorp...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-015-6856-y
更新日期：2015-12-01 00:00:00
abstract：:The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel β-coronavirus, is the main pathogenic agent of the rapidly spreading pneumonia called coronavirus disease 2019 (COVID-19). SARS-CoV-2 infects much more people, especially the elder population, around the world than other coronavirus, such as SAR...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-020-10814-6
更新日期：2020-09-01 00:00:00
abstract：:The engineered Saccharomyces cerevisiae strain △faa1△faa4 [Acot5s] was demonstrated to accumulate more free fatty acids (FFA) previously. Here, comparative proteomic analysis was performed to get a global overview of metabolic regulation in the strain. Over 500 proteins were identified, and 82 of those proteins were f...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-015-7028-9
更新日期：2016-02-01 00:00:00
abstract：:A new pmoA gene-based PCR primer set was designed for detection of nitrite-dependent anaerobic oxidation of methane (n-damo) bacteria from four different ecosystems, namely rice paddy soil, freshwater reservoir, reed bed, and sludge from wastewater treatment plant. This primer set showed high specificity and efficienc...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-013-5260-8
更新日期：2013-12-01 00:00:00
abstract：:D-Tagatose is a hexoketose monosaccharide sweetener, which is an isomer of D-galactose and is rarely found in nature. Recently, there has been industrial interest in D-tagatose as a low-calorie sugar-substituting sweetener. This article describes the properties and metabolism of tagatose as well as its commercial impo...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-004-1665-8
更新日期：2004-08-01 00:00:00
abstract：:A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/BF00184696
更新日期：1991-05-01 00:00:00
abstract：:A phylogeny-guided approach was applied to screen endophytic fungi containing type I polyketide synthase (PKS I) biosynthetic gene sequences and aimed to correlate genotype to chemotype for the discovery of novel bioactive polyketides. Salicorn 58, which was identified as Talaromyces funiculosus based on its internal ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7311-4
更新日期：2016-06-01 00:00:00
abstract：:Alkalophilic Bacillus sp. KSM-K16 produced three alkaline proteases, as detected by polyacrylamide gel electrophoresis (PAGE). The major protease, designated M protease, was recently purified to homogeneity and its properties were characterized. In the present study, two minor proteases, designated H protease and N pr...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530050650
更新日期：1996-03-01 00:00:00
abstract：:Bacterial glucans have aroused increasing interest in commercial applications in the food and pharmaceutical sectors. A number of bacterial glucans have been reported over recent decades, and their structure, production, and functional properties have been extensively studied. In this paper, we review recent researche...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-016-7836-6
更新日期：2016-11-01 00:00:00
abstract：:A novel immobilisation design for increasing the final concentration of the heterologous protein lysozyme by a genetically engineered fungus, Aspergillus niger B1, was developed. A central composition design was used to investigate different immobilised polymer types (alginate and pectate), polymer concentration [24% ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-004-1742-z
更新日期：2005-05-01 00:00:00
abstract：:2-Phenylethanol (2-PE) is an important flavor compound but also impairs cell growth severely, which in turn blocks its bioproduction. However, the molecular mechanism of 2-PE tolerance is unclear. In this study, a superb 2-PE stress-tolerant and producing yeast, Candida glycerinogenes, was selected to uncover the unde...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-020-10509-y
更新日期：2020-05-01 00:00:00
abstract：:In order to understand the intracellular responses in recombinant CHO (rCHO) cells producing antibody in serum-free medium (SFM) supplemented with optimized hydrolysates mixtures, yielding the highest specific growth rate (μ, SFM#S1) or the highest specific antibody productivity (q(Ab,) SFM#S2), differentially express...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3106-9
更新日期：2011-03-01 00:00:00
abstract：:Brazzein is an intensely sweet-tasting plant protein with good stability, which makes it an attractive alternative to sucrose. A brazzein gene has been designed, synthesized, and expressed in Escherichia coli at 30 degrees C to yield brazzein in a soluble form and in considerable quantity. Antibodies have been produce...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-006-0438-y
更新日期：2006-11-01 00:00:00
abstract：:L-cysteine, a valuable sulfur-containing amino acid, has been widely used in food, agriculture, and pharmaceutical industries. Due to the toxicity and complex regulation of L-cysteine, no efficient cell factory has yet been achieved for L-cysteine industrial production. In this study, the food-grade microorganism Cory...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-018-9547-7
更新日期：2019-02-01 00:00:00
abstract：:To meet the demand for sustainable energy, aquifer thermal energy storage (ATES) is widely used in the subsurface in urban areas. However, contamination of groundwater, especially with chlorinated volatile organic compounds (CVOCs), is often being encountered. This is commonly seen as an impediment to ATES implementat...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-015-7241-6
更新日期：2016-04-01 00:00:00
abstract：:Potentiometric biosensors for the determination of organophosphorus (OP) insecticides were developed by applying either immobilized whole cells or cytoplasmic membrane fractions of wild-type Flavobacterium sp. on the surface of a glass pH electrode. The ability of Flavobacterium sp. to degrade OP compounds as sole car...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530000437
更新日期：2000-11-01 00:00:00
abstract：:Gelatin zymography revealed the presence of proteolytic activity in conditioned medium (CM) from a serum-free, non-infected Spodoptera frugiperda, Sf9 insect cell culture. Two peptidase bands at about 49 and 39 kDa were detected and found to be proform and active form of the same enzyme. The 49-kDa form was visible on...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-005-0181-9
更新日期：2006-07-01 00:00:00
abstract：:The original version of this article was revised: After publication of this article, the publisher was notified that Michael Klocke has been listed as an author without his consent. ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00253-019-10137-1
更新日期：2019-12-01 00:00:00
abstract：:Porcine parvovirus (PPV) virus-like particles (VLPs) constitute a potential vaccine for prevention of parvovirus-induced reproductive failure in gilts. Here we report the development of a large scale (25 l) production process for PPV-VLPs with baculovirus-infected insect cells. A low multiplicity of infection (MOI) st...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-002-0976-x
更新日期：2002-06-01 00:00:00
abstract：:Damages of the (agro)ecosystem by extensive use of chemical fertilizers and pesticides, the global dying of bee populations possibly linked to pesticide spraying, and stricter regulations for pesticide use together with successful use of microbials in IPM programs are pushing on the development and commercialization o...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-018-9173-4
更新日期：2018-09-01 00:00:00
abstract：:The thermostable beta-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding p-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyrano...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530100627
更新日期：2001-08-01 00:00:00
abstract：:In the Funding section, the following statement is missing: The MACS cohort study was supported by the NIH, National Institute of Allergy and Infectious Diseases grant U01-AI35041. ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00253-019-09911-y
更新日期：2019-07-01 00:00:00
