当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > A novel immobilised design for the production of the heterologous protein lysozyme by a genetically engineered Aspergillus niger strain.

A novel immobilised design for the production of the heterologous protein lysozyme by a genetically engineered Aspergillus niger strain.

Abstract：

:A novel immobilisation design for increasing the final concentration of the heterologous protein lysozyme by a genetically engineered fungus, Aspergillus niger B1, was developed. A central composition design was used to investigate different immobilised polymer types (alginate and pectate), polymer concentration [24% and 4% (w/v)], inoculum support ratios (1:2 and 1:4) and gel-inducing agent concentration [CaCl(2), 2% and 3.5% (w/v)]. Studies of the kinetics of production showed that optimum lysozyme productivity occurred after 10 days. Lysozyme production was significantly affected by polymer type, polymer concentration, and inoculum support ratio. Overall, immobilisation in Ca-pectate resulted in higher lysozyme production compared to that in Ca-alginate. Similar effects were observed when the polymer concentration was reduced. Regardless of polymer type and concentration, increasing the fungal inoculum level increased lysozyme production. A significantly higher lysozyme yield was achieved with Ca-pectate in comparison to Ca-alginate (approximately 20-23 mg l(-1) and 0.5-2 mg l(-1), respectively). The maximum lysozyme yield achieved was about 23 mg l(-1) by immobilisation in Ca-pectate 2% (w/v) with 33% (v/v) mycelium and 3.5% (w/v) gel-inducing agent (CaCl(2)). Response surface methodology was used to investigate the effect of pH and water activity (a(w)). The best medium pH was 4.5-5.0, and bead a(w) for optimum lysozyme yield was 0.94, regardless of polymer type.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Parra R,Aldred D,Magan N

doi

10.1007/s00253-004-1742-z

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

336-44

issue

3

eissn

0175-7598

issn

1432-0614

journal_volume

67

pub_type

杂志文章

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