Application of an amperometric immunosensor for the enumeration of Nitrobacter in activated sludge.

abstract：:Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may pro...

journal_title：Applied microbiology and biotechnology

authors： Silva ALP,da Silva Caridade TN,Magalhães RR,de Sousa KT,de Sousa CC,Vale JA

更新日期：2020-10-01 00:00:00

abstract：:A single-stage continuous fermentation process for the production of 2-keto-L-gulonic acid (2KGA) from L-sorbose using Ketogulonigenium vulgare DSM 4025 was developed. The chemostat culture with the dilution rate that was calculated based on the relationship between the 2KGA production rate and the 2KGA concentration ...

journal_title：Applied microbiology and biotechnology

authors： Takagi Y,Sugisawa T,Hoshino T

更新日期：2009-04-01 00:00:00

abstract：:The wide application of water-soluble linear polyacrylamides (PAMs) can cause serious environmental pollution. Biological treatment of PAMs receives very limited efficiency due to their recalcitrance to the microbial degradation. Here, we show the bioelectrochemical system (BES) can be used as an effective strategy to...

journal_title：Applied microbiology and biotechnology

authors： Cui YZ,Zhang J,Sun M,Zhai LF

更新日期：2015-01-01 00:00:00

abstract：:A characteristic mucosal immune response involves the production of antigen-specific secretory immunoglobulin A (SIgA) antibodies. In order to study transcytosis by mimicking the SIgA secretion and to screen for SIgA secretion-promoting substances, we developed a model system of a transfected Madin-Darby canine kidney...

journal_title：Applied microbiology and biotechnology

authors： Zuo T,Feng X,Zhang N,Xue C,Tang QJ

更新日期：2015-07-01 00:00:00

abstract：:Various techniques are used to adjust the flavors of foods and beverages to new market demands. Although synthetic flavoring chemicals are still widely used, flavors produced by biological methods (bioflavors) are now more and more requested by consumers, increasingly concerned with health and environmental problems c...

journal_title：Applied microbiology and biotechnology

authors： Vanderhaegen B,Neven H,Coghe S,Verstrepen KJ,Derdelinckx G,Verachtert H

更新日期：2003-08-01 00:00:00

abstract：:In oyster ponds, the marine diatom Haslea ostrearia synthesises and excretes a hydrosoluble pigment of commercial interest called marennin. During the benthic stage, when algal cells are naturally immobilised in their own polysaccharides, marennin production is higher. To optimise this production, axenic cultures of H...

journal_title：Applied microbiology and biotechnology

authors： Lebeau T,Gaudin P,Moan R,Robert JM

更新日期：2002-07-01 00:00:00

abstract：:Bacillus thuringiensis bacteria show insecticidal activities that rely upon the production of insecticidal crystal proteins, which are encoded by cry or cyt genes and can target a variety of insect pests. It has been shown that cry1Ac is the only cry gene in B. thuringiensis subsp. kurstaki HD73 (B. thuringiensis HD73...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Gao T,Peng Q,Song L,Zhang J,Chai Y,Sun D,Song F

更新日期：2018-04-01 00:00:00

abstract：:In microorganisms, the enzyme acetate kinase (AK) catalyses the formation of ATP from ADP by de-phosphorylation of acetyl phosphate into acetic acid. A mutant strain of Clostridium acetobutylicum lacking acetate kinase activity is expected to have reduced acetate and acetone production compared to the wild type. In th...

journal_title：Applied microbiology and biotechnology

authors： Kuit W,Minton NP,López-Contreras AM,Eggink G

更新日期：2012-05-01 00:00:00

abstract：:Photosynthetic microorganisms produce relatively large amounts of physiologically active materials which stimulate the physiological activity of other organisms. In this study, mammalian HeLa cells were cultured in different culture media which were Dulbecco's modified Eagle medium (DMEM) with newborn calf serum (NCS)...

journal_title：Applied microbiology and biotechnology

authors： Lee HJ,Park JY,Yoo KS,Yoon J,Kim YH,Min J

更新日期：2013-11-01 00:00:00

abstract：:Trichothecenes are toxic secondary metabolites produced by filamentous fungi mainly belonging to the Fusarium genus. Production of these mycotoxins occurs during infection of crops and is a threat to human and animal health. Although the pathway for biosynthesis of trichothecenes is well established, the regulation of...

journal_title：Applied microbiology and biotechnology

authors： Merhej J,Richard-Forget F,Barreau C

更新日期：2011-08-01 00:00:00

abstract：:The paper describes the synthesis of the phosphorylcholine-binding miniantibody McPC603scFvDhl x in cell-wall-less L-form strains of Escherichia coli and Proteus mirabilis. Cells of these strains were transformed with the plasmid pACK02scKan, carrying the miniantibody (miniAb) coding sequence under the control of the ...

journal_title：Applied microbiology and biotechnology

authors： Kujau MJ,Hoischen C,Riesenberg D,Gumpert J

更新日期：1998-01-01 00:00:00

abstract：:Bacteria that grow on p-xylene, p-toluic acid, and terephthalic acid (TPA) were isolated from a wastewater bioreactor that is used to treat a waste stream that contains all three of these compounds. Although previously described aerobic bacteria degrade p-xylene by initially oxidizing a single methyl group to form p-t...

journal_title：Applied microbiology and biotechnology

authors： Bramucci MG,McCutchen CM,Singh M,Thomas SM,Larsen BS,Buckholz J,Nagarajan V

更新日期：2002-02-01 00:00:00

abstract：:Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environments, carbohydrates are converted into volatile fatty acids and alcohols. These can be used as building blocks in biotechnological or chemical processes, e.g., to produce bioplastics. In this study, xylose fermentation by mixed m...

journal_title：Applied microbiology and biotechnology

authors： Temudo MF,Mato T,Kleerebezem R,van Loosdrecht MC

更新日期：2009-02-01 00:00:00

abstract：:Severe dengue is more likely found during secondary heterologous dengue virus (DENV) infection or primary infection of infants born to dengue-immune mothers and led to the hypothesis of antibody-dependent enhancement (ADE). It has been reported that pre-membrane (prM)-reactive antibodies do not efficiently neutralize ...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Si L,Luo Y,Guo X,Zhou J,Fang D,Yan H,Zeng G,Jiang L

更新日期：2015-11-01 00:00:00

abstract：:Discovery of keratin-degrading enzymes from fungi and bacteria has primarily focused on finding one protease with efficient keratinase activity. Recently, an investigation was conducted of all keratinases secreted from a fungus known to grow on keratinaceous materials, such as feather, horn, and hooves. The study demo...

journal_title：Applied microbiology and biotechnology

authors： Lange L,Huang Y,Busk PK

更新日期：2016-03-01 00:00:00

abstract：:A gene for a synthetic protein-based polymer, G-(VPGVG)119-VPGV, coding for the EG-120mer (elastomer), was cloned into a fungal expression vector to allow constitutive expression of the polymer controlled by the gpdA (glyceraldehyde-3-phosphate dehydrogenase) promoter sequence of Aspergillus nidulans. Stable transform...

journal_title：Applied microbiology and biotechnology

authors： Herzog RW,Singh NK,Urry DW,Daniell H

更新日期：1997-04-01 00:00:00

abstract：:Xylanase is the enzyme complex that is responsible for the degradation of xylan; however, novel xylanase producers remain to be explored in marine environment. In this study, a Streptomyces strain M11 which exhibited xylanase activity was isolated from marine sediment. The 16S rDNA sequence of M11 showed the highest i...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Zhao X,Bai F

更新日期：2013-05-01 00:00:00

abstract：:A beta-lactamase-free penicillin amidase from Alcaligenes sp. active against various beta-lactams was purified to homogeneity. The enzyme can hydrolyze penicillin G to 6-amino penicillanic acid (6-APA) and furnish penicillin G from 6-APA and phenyl acetic acid by condensation. The penicillin amidase is a heterodimer o...

journal_title：Applied microbiology and biotechnology

authors： Das S,Gayen JR,Pal A,Ghosh K,Rosazza JP,Samanta TB

更新日期：2004-08-01 00:00:00

abstract：:The basidiomycetous fungus Pseudozyma aphidis is able to convert vegetable oils to abundant amounts of the biosurfactant mannosylerythritol lipid (MEL) with a unique product pattern of MEL-A, MEL-B, MEL-C, and MEL-D. To investigate the metabolism of MEL production, we analyzed the transcriptome of P. aphidis DSM 70725...

journal_title：Applied microbiology and biotechnology

authors： Günther M,Grumaz C,Lorenz S,Stevens P,Lindemann E,Hirth T,Sohn K,Zibek S,Rupp S

更新日期：2015-02-01 00:00:00

abstract：:Using a randomly-cloned, HindIII-digested, chromosomal fragment from Lactococcus lactis subsp. lactis LM0230, first-generation lactococcal integrative cloning vectors were developed. Through dideoxy DNA sequence analysis, the cloned chromosomal DNA fragment was determined to be 1026 base pairs. Southern hybridization ...

journal_title：Applied microbiology and biotechnology

authors： McIntyre DA,Harlander SK

更新日期：1993-11-01 00:00:00

abstract：:Denitrifying sulfide removal (DSR) process simultaneously converts sulfide, nitrate, and chemical oxygen demand from industrial wastewaters to elemental sulfur, nitrogen gas, and carbon dioxide, respectively. This investigation utilizes a dilution-to-extinction approach at 10(-2) to 10(-6) dilutions to elucidate the c...

journal_title：Applied microbiology and biotechnology

authors： Chen C,Ren N,Wang A,Liu L,Lee DJ

更新日期：2010-03-01 00:00:00

abstract：:A bench-scale granular autotrophic nitrogen removal bioreactor (completely autotrophic nitrogen removal over nitrite (CANON) system) used for the treatment of synthetic wastewater was analyzed for the identification of microbiota with potential capacity for carbonate and phosphate biomineral formation. 16S ribosomal R...

journal_title：Applied microbiology and biotechnology

authors： Gonzalez-Martinez A,Rodriguez-Sanchez A,Rivadeneyra MA,Rivadeneyra A,Martin-Ramos D,Vahala R,Gonzalez-Lopez J

更新日期：2017-01-01 00:00:00

abstract：:Microbes engineered to display heterologous proteins could be useful biotechnological tools for protein engineering, lignocellulose degradation, biocatalysis, bioremediation, and biosensing. Bacillus subtilis is a promising host to display proteins, as this model Gram-positive bacterium is genetically tractable and al...

journal_title：Applied microbiology and biotechnology

authors： Huang GL,Gosschalk JE,Kim YS,Ogorzalek Loo RR,Clubb RT

更新日期：2018-08-01 00:00:00

abstract：:Soluble expression of recombinant therapeutic proteins in Escherichia coli (E. coli) has been a challenging task in biopharmaceutical development. In this study, a novel self-cleavable tag Zbasic-intein has been constructed for the soluble expression and purification of a recombinant cytokine, human interleukin-15 (IL...

journal_title：Applied microbiology and biotechnology

authors： Shi S,Chen H,Jiang H,Xie Y,Zhang L,Li N,Zhu C,Chen J,Luo H,Wang J,Feng L,Lu H,Zhu J

更新日期：2017-02-01 00:00:00

abstract：:While diatoms are widely present in terms of diversity and abundance in nature, few species are currently used for biotechnologically applications. Most studies have focussed on intracellularly synthesised eicosapentaenoic acid (EPA), a polyunsaturated fatty acid (PUFA) used for pharmaceutical applications. Applicatio...

journal_title：Applied microbiology and biotechnology

authors： Lebeau T,Robert JM

更新日期：2003-02-01 00:00:00

abstract：:Probiotics and bovine colostrums had been proven to be beneficial for human health. Lactobacillus rhamnosus ZDY114 and anti-Helicobacter pylori bovine colostrums were used for the preparation of microecological additives, and their synergistic antidigestion effect in the simulated gastrointestinal tract (in vitro) was...

journal_title：Applied microbiology and biotechnology

authors： Wei H,Xu Y,Xiong Y,Xu F,Liu G

更新日期：2007-06-01 00:00:00

abstract：:The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration b...

journal_title：Applied microbiology and biotechnology

authors： Valera MJ,Torija MJ,Mas A,Mateo E

更新日期：2015-02-01 00:00:00

abstract：:The microbial production of biofuels is a promising avenue for the development of viable processes for the generation of fuels from sustainable resources. In order to become cost and energy effective, these processes must utilize organisms that can be optimized to efficiently produce candidate fuels from a variety of ...

journal_title：Applied microbiology and biotechnology

authors： Clomburg JM,Gonzalez R

更新日期：2010-03-01 00:00:00

abstract：:The process of succinic acid (SA) production represents the combination of microbial synthesis of α-ketoglutaric acid from rapeseed oil by yeast Yarrowia lipolytica VKM Y-2412 and subsequent decarboxylation of α-ketoglutaric acid by hydrogen peroxide to SA that leads to the production of 69.0 g l(-1) of SA and 1.36 g ...

journal_title：Applied microbiology and biotechnology

authors： Kamzolova SV,Vinokurova NG,Dedyukhina EG,Samoilenko VA,Lunina JN,Mironov AA,Allayarov RK,Morgunov IG

更新日期：2014-05-01 00:00:00

abstract：:A survey in 16 activated sludge wastewater treatment plants (WWTP) was conducted to contribute to the knowledge of the environmental parameters that determine the composition of the filamentous community. A total of 128 samples of mixed liquor from municipal WWTP were collected during 2 years, and 22 filamentous morph...

journal_title：Applied microbiology and biotechnology

authors： Araújo Dos Santos L,Ferreira V,Neto MM,Pereira MA,Mota M,Nicolau A

更新日期：2015-06-01 00:00:00