Establishment of a functional secretory IgA transcytosis model system in vitro for functional food screening.


:A characteristic mucosal immune response involves the production of antigen-specific secretory immunoglobulin A (SIgA) antibodies. In order to study transcytosis by mimicking the SIgA secretion and to screen for SIgA secretion-promoting substances, we developed a model system of a transfected Madin-Darby canine kidney (MDCK) cell line that expresses the human polymeric immunoglobulin receptor (pIgR). We thus isolated the human dIgA (dimeric IgA)/pIgA (polymeric IgA) complex as the binding ligands. In the present study, a recombinant vector encoding the human pIgR gene was constructed and infected into MDCK cells. Following reverse transcription polymerase chain reaction (RT-PCR), immunoblotting, and immunofluorescence staining, we confirmed that pIgR was expressed in the transfectant MDCK-pIgR cells and was located at the basolateral side of the cell surface. We also confirmed the coexistence of the dIgA/pIgA complex in the IgA myeloma serum. The covalent dIgA/pIgA complex was then isolated from the serum of an IgA multiple myeloma patient using an ÄKTA purifier operation system with a HiPrep 16/60 Sephacryl S-300 HR column, in order to utilize the complex as transcytosis ligands for human pIgR. Finally, we confirmed the uptake of the isolated human dIgA/pIgA complex into MDCK-pIgR cells. We demonstrated that the human dIgA/pIgA complex was transcytosed into the apical side of the monolayer cells. Therefore, our MDCK-human pIgR cell transcytosis model is an operational system and can be used for screening functional food components that promote dIgA/pIgR transcytosis as well as SIgA secretion.


Zuo T,Feng X,Zhang N,Xue C,Tang QJ




Has Abstract


2015-07-01 00:00:00












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    abstract::The human cell line rF2N78 produces an antibody with a high galactosylation ratio which resembles human IgG. However, it has been observed that the aglycosylated antibody starts to appear when glucose is depleted. To determine whether glucose depletion is a main cause for aglycosylation of the antibody, fed-batch cult...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Seo JS,Min BS,Kim YJ,Cho JM,Baek E,Cho MS,Lee GM

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  • Soil acidification amendments change the rhizosphere bacterial community of tobacco in a bacterial wilt affected field.

    abstract::Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Shen G,Zhang S,Liu X,Jiang Q,Ding W

    更新日期:2018-11-01 00:00:00

  • Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034.

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    journal_title:Applied microbiology and biotechnology

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    authors: Ruiz Rodríguez LG,Aller K,Bru E,De Vuyst L,Hébert EM,Mozzi F

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  • Improving extracellular protein production in Escherichia coli by overexpressing D,D-carboxypeptidase to perturb peptidoglycan network synthesis and structure.

    abstract::Most recombinant proteins in Escherichia coli are not efficiently secreted to the extracellular space. Structural stabilisation of the cell wall is essential for extracellular protein production in E. coli, for which D,D-carboxypeptidases are essential. Herein, we perturbed the peptidoglycan structure of the E. coli c...

    journal_title:Applied microbiology and biotechnology

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    authors: Yang H,Hu J,Lu X,Wang F,Shen W,Hu W,Wang L,Chen X,Liu L

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Salerno AJ,Goldberg I

    更新日期:1993-05-01 00:00:00

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Günther M,Grumaz C,Lorenz S,Stevens P,Lindemann E,Hirth T,Sohn K,Zibek S,Rupp S

    更新日期:2015-02-01 00:00:00

  • Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches.

    abstract::Due to the increasing demand for recombinant proteins, the interest in mammalian cell culture, especially of Chinese hamster ovary cells, grows rapidly. This is accompanied by the desire to improve cell lines in order to achieve higher titers and a better product quality. Until recently, most cell line development pro...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Krämer O,Klausing S,Noll T

    更新日期:2010-09-01 00:00:00

  • Directed evolution of a Baeyer-Villiger monooxygenase to enhance enantioselectivity.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kirschner A,Bornscheuer UT

    更新日期:2008-12-01 00:00:00

  • Expression and characterization of a thermostable sarcosine oxidase (SOX) from Bacillus sp. in Escherichia coli.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Guo K,Ma X,Sun G,Zhao Y,Li X,Zhao W,Kai L

    更新日期:2006-12-01 00:00:00

  • Identification and characterization of the propanediol utilization protein PduP of Lactobacillus reuteri for 3-hydroxypropionic acid production from glycerol.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Luo LH,Seo JW,Baek JO,Oh BR,Heo SY,Hong WK,Kim DH,Kim CH

    更新日期:2011-02-01 00:00:00

  • Biomass production and nutrient uptake by Neochloris oleoabundans in an open trough system.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Murray KE,Healy FG,McCord RS,Shields JA

    更新日期:2011-04-01 00:00:00

  • Biofilms of As(III)-oxidising bacteria: formation and activity studies for bioremediation process development.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Michel C,Jean M,Coulon S,Dictor MC,Delorme F,Morin D,Garrido F

    更新日期:2007-11-01 00:00:00

  • DO-stat fed-batch production of 2-keto-D-gluconic acid from cassava using immobilized Pseudomonas aeruginosa.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Chia M,Van Nguyen TB,Choi WJ

    更新日期:2008-04-01 00:00:00

  • Replacement of pr gene with Japanese encephalitis virus pr using reverse genetics reduces antibody-dependent enhancement of dengue virus 2 infection.

    abstract::Severe dengue is more likely found during secondary heterologous dengue virus (DENV) infection or primary infection of infants born to dengue-immune mothers and led to the hypothesis of antibody-dependent enhancement (ADE). It has been reported that pre-membrane (prM)-reactive antibodies do not efficiently neutralize ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Wang Y,Si L,Luo Y,Guo X,Zhou J,Fang D,Yan H,Zeng G,Jiang L

    更新日期:2015-11-01 00:00:00

  • Microbiota composition of captive bluestreak cleaner wrasse Labroides dimidiatus (Valenciennes, 1839).

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Nurul AAN,Danish-Daniel AM,Okomoda VT,Asma NA

    更新日期:2020-09-01 00:00:00

  • Phosphate starvation controls lactose metabolism to produce recombinant protein in Escherichia coli.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Pandi K,Chauhan AS,Khan WH,Rathore AS

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  • The effects of upaB deletion and the double/triple deletion of upaB, aatA, and aatB genes on pathogenicity of avian pathogenic Escherichia coli.

    abstract::Autotransporters (ATs) are associated with pathogenesis of Avian Pathogenic Escherichia coli (APEC). The molecular characterization of APEC ATs can provide insights about their relevance to APEC pathogenesis. Here, we characterized a conventional autotransporter UpaB in APEC DE205B genome. The upaB existed in 41.9 % o...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhu-Ge XK,Pan ZH,Tang F,Mao X,Hu L,Wang SH,Xu B,Lu CP,Fan HJ,Dai JJ

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  • Substrate specificity of Stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid.

    abstract::An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regi...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Yu IS,Yeom SJ,Kim HJ,Lee JK,Kim YH,Oh DK

    更新日期:2008-02-01 00:00:00

  • Arabinoxylan and mono- and dimeric ferulic acid release from brewer's grain and wheat bran by feruloyl esterases and glycosyl hydrolases from Humicola insolens.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Faulds CB,Mandalari G,LoCurto R,Bisignano G,Waldron KW

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  • A new DNA polymerase I from Geobacillus caldoxylosilyticus TK4: cloning, characterization, and mutational analysis of two aromatic residues.

    abstract::DNA polymerase I gene was cloned and sequenced from the thermophilic bacterium Geobacillus caldoxylosilyticus TK4. The gene is 2,634 bp long and encodes a protein of 878 amino acids in length. The enzyme has a molecular mass of 99 kDa and shows sequence homology with DNA polymerase I from Bacillus species (89% identit...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Sandalli C,Singh K,Modak MJ,Ketkar A,Canakci S,Demir I,Belduz AO

    更新日期:2009-08-01 00:00:00

  • Cloning and expression of the L-1-amino-2-propanol dehydrogenase gene from Rhodococcus erythropolis, and its application to double chiral compound production.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kataoka M,Ishige T,Urano N,Nakamura Y,Sakuradani E,Fukui S,Kita S,Sakamoto K,Shimizu S

    更新日期:2008-09-01 00:00:00

  • Alteration in cell surface properties of Burkholderia spp. during surfactant-aided biodegradation of petroleum hydrocarbons.

    abstract::Chemical surfactants may impact microbial cell surface properties, i.e., cell surface hydrophobicity (CSH) and cell surface charge, and may thus affect the uptake of components from non-aqueous phase liquids (NAPLs). This work explored the impact of Triton X-100, Igepal CA 630, and Tween 80 (at twice the critical mice...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Mohanty S,Mukherji S

    更新日期:2012-04-01 00:00:00

  • Correction to: Prokaryotic expression and action mechanism of antimicrobial LsGRP1C recombinant protein containing a fusion partner of small ubiquitin-like modifier.

    abstract::The published online version contains editing mistake in Table 2. See below for the corrected Table. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,已发布勘误


    authors: Lin CH,Pan YC,Liu FW,Chen CY

    更新日期:2018-01-01 00:00:00

  • Removal of tetrachloroethylene in an anaerobic column bioreactor.

    abstract::Removal of tetrachloroethylene (perchloroethylene; C2Cl4) by microbial consortia from two sites with different C2Cl4 exposure histories was examined in a bench-scale anaerobic column bioreactor. It was hypothesized that optimal removal would be observed in the reactor packed with sediments having an extensive exposure...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Noftsker C,Watwood ME

    更新日期:1997-09-01 00:00:00

  • Improved homo L-lactic acid fermentation from xylose by abolishment of the phosphoketolase pathway and enhancement of the pentose phosphate pathway in genetically modified xylose-assimilating Lactococcus lactis.

    abstract::In order to achieve efficient homo L-lactic acid fermentation from xylose, we first carried out addition of xylose assimilation ability to Lactococcus lactis IL 1403 by introducing a plasmid carrying the xylRAB genes from L. lactis IO-1 (pXylRAB). Then modification of xylose assimilation pathway was carried out. L. la...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Shinkawa S,Okano K,Yoshida S,Tanaka T,Ogino C,Fukuda H,Kondo A

    更新日期:2011-09-01 00:00:00

  • 16S rRNA gene-based characterization of bacteria potentially associated with phosphate and carbonate precipitation from a granular autotrophic nitrogen removal bioreactor.

    abstract::A bench-scale granular autotrophic nitrogen removal bioreactor (completely autotrophic nitrogen removal over nitrite (CANON) system) used for the treatment of synthetic wastewater was analyzed for the identification of microbiota with potential capacity for carbonate and phosphate biomineral formation. 16S ribosomal R...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Gonzalez-Martinez A,Rodriguez-Sanchez A,Rivadeneyra MA,Rivadeneyra A,Martin-Ramos D,Vahala R,Gonzalez-Lopez J

    更新日期:2017-01-01 00:00:00

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Xia C,Li Z,Xu Y,Yang P,Gao L,Yan Q,Li S,Wang Y,Qu Y,Song X

    更新日期:2019-03-01 00:00:00

  • Comparative studies on similarities and differences of cyclodipeptide oxidases for installation of C-C double bonds at the diketopiperazine ring.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Mikulski L,Schäfer J,Brockmeyer K,Kraut R,Li SM

    更新日期:2020-03-01 00:00:00

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Akhtar MK,Jones PR

    更新日期:2008-04-01 00:00:00

  • Production of Lactobacillus kefir cells for asymmetric synthesis of a 3,5-dihydroxycarboxylate.

    abstract::An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% (3.7 g(DCW) l(-1)-day(-1)) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7--9 mmol l(-1) h(-1). Th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Pfruender H,Amidjojo M,Hang F,Weuster-Botz D

    更新日期:2005-06-01 00:00:00