Establishment of a functional secretory IgA transcytosis model system in vitro for functional food screening.

abstract：:There is an error in the Original Publication of this paper for "Acknowledgements" section was missing. ...

journal_title：Applied microbiology and biotechnology

authors： Hauschild P,Röttig A,Madkour MH,Al-Ansari AM,Almakishah NH,Steinbüchel A

更新日期：2020-03-01 00:00:00

abstract：:The extrusion technology of blends formed by compounds with different physicochemical properties often results in new materials that present properties distinctive from its original individual constituents. Here, we report the use of melt extrusion of blends made from low-cost materials to produce a biodegradable foam...

journal_title：Applied microbiology and biotechnology

authors： Marcelino PR,Milani KM,Mali S,Santos OJ,de Oliveira AL

更新日期：2016-08-01 00:00:00

abstract：:Rhizopus oryzae is a filamentous fungus belonging to the Zygomycetes. It is among others known for its ability to produce the sustainable platform chemicals L: -(+)-lactic acid, fumaric acid, and ethanol. During glycolysis, all fermentable carbon sources are metabolized to pyruvate and subsequently distributed over th...

journal_title：Applied microbiology and biotechnology

authors： Meussen BJ,de Graaff LH,Sanders JP,Weusthuis RA

更新日期：2012-05-01 00:00:00

abstract：:The efficient fermentation of xylose can improve biofuel production. We previously developed a two-stage transcriptional reprogramming (TSTR) strategy (including a glucose fermentation stage and a xylose fermentation stage) and demonstrated its application for the construction of Saccharomyces cerevisiae strains with ...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Wang J,Zhang W,Hou JY,Xiao W,Cao L

更新日期：2018-08-01 00:00:00

abstract：:Functional genomics of filamentous fungi has gradually uncovered gene information for constructing 'cell factories' and controlling pathogens. Available gene manipulation methods of filamentous fungi include random integration methods, gene targeting technology, gene editing with artificial nucleases and RNA technolog...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Chen H,Tang X,Zhang H,Chen W,Chen YQ

更新日期：2017-11-01 00:00:00

abstract：:Dyes and dyestuffs find use in a wide range of industries but are of primary importance to textile manufacturing. Wastewater from the textile industry can contain a variety of polluting substances including dyes. Increasingly, environmental legislation is being imposed to control the release of dyes, in particular azo...

journal_title：Applied microbiology and biotechnology

authors： McMullan G,Meehan C,Conneely A,Kirby N,Robinson T,Nigam P,Banat IM,Marchant R,Smyth WF

更新日期：2001-07-01 00:00:00

abstract：:The biomass carbon ratios of biochemicals related to biomass have been reviewed. Commercial products from biomass were explained. The biomass carbon ratios of biochemical compounds were measured by accelerator mass spectrometry (AMS) based on the (14)C concentration of carbons in the compounds. This measuring method u...

journal_title：Applied microbiology and biotechnology

authors： Kunioka M

更新日期：2010-06-01 00:00:00

abstract：:The human cell line rF2N78 produces an antibody with a high galactosylation ratio which resembles human IgG. However, it has been observed that the aglycosylated antibody starts to appear when glucose is depleted. To determine whether glucose depletion is a main cause for aglycosylation of the antibody, fed-batch cult...

journal_title：Applied microbiology and biotechnology

authors： Seo JS,Min BS,Kim YJ,Cho JM,Baek E,Cho MS,Lee GM

更新日期：2014-04-01 00:00:00

abstract：:Programmable transcriptional regulation is a powerful tool to study gene functions. Current methods to selectively regulate target genes are mainly based on promoter exchange or on overexpressing transcriptional activators. To expand the discovery toolbox, we designed a dCas9-based RNA-guided synthetic transcription a...

journal_title：Applied microbiology and biotechnology

authors： Schüller A,Wolansky L,Berger H,Studt L,Gacek-Matthews A,Sulyok M,Strauss J

更新日期：2020-11-01 00:00:00

abstract：:Lipoxygenase (LOX; EC 1.13.11.12) is an enzyme which is widely used in food industry to improve aroma and rheological or baking properties of foods. A series of studies have proven that the flexible regions negatively relates to the thermal stability of enzymes. In this study, two highly flexible regions, residues(20-...

journal_title：Applied microbiology and biotechnology

authors： Lu X,Liu S,Feng Y,Rao S,Zhou X,Wang M,Du G,Chen J

更新日期：2014-02-01 00:00:00

abstract：:The first two genes of the threonine pathway, ask and asd, were cloned and sequenced from the aminoethoxyvinylglycine-producing Streptomyces sp. NRRL 5331. The two genes are organized in a bicistronic operon. ask, encoding the apartokinase (ASK), is located upstream from asd. The presence of a ribosome-binding site wi...

journal_title：Applied microbiology and biotechnology

authors： Cuadrado Y,Fernández M,Recio E,Aparicio JF,Martín JF

更新日期：2004-04-01 00:00:00

abstract：:Three cytosolic NADPH-dependent flavin-associated proteins (Gox2107, Gox0502, and Gox2684) from Gluconobacter oxydans 621H were overproduced in Escherichia coli, and the recombinant enzymes were purified and characterized. Apparent native molecular masses of 65.2, 78.2, and 78.4 kDa were observed for Gox2107, Gox0502,...

journal_title：Applied microbiology and biotechnology

authors： Schweiger P,Gross H,Wesener S,Deppenmeier U

更新日期：2008-10-01 00:00:00

abstract：:We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region ...

journal_title：Applied microbiology and biotechnology

authors： Piret J,Resendiz B,Mahro B,Zhang JY,Serpe E,Romero J,Connors N,Demain AL

更新日期：1990-02-01 00:00:00

abstract：:A heat-stable sarcosine oxidase produced by Bacillus sp. BSD-8 (SOX) had been studied and its complete gene sequence, which contained 1,164 bp nucleotides and encoded a protein of 387 amino acids, was obtained by DNA Walking method. The sox gene was cloned and functionally overexpressed in E. coli and the recombinant ...

journal_title：Applied microbiology and biotechnology

authors： Guo K,Ma X,Sun G,Zhao Y,Li X,Zhao W,Kai L

更新日期：2006-12-01 00:00:00

abstract：:Media and growth conditions were optimized for the microaerobic cultivation of Magnetospirillum gryphiswaldense in flasks and in a fermentor, resulting in significantly increased cell and magnetosome yields, compared with earlier studies. A reliable method was established for the automatic control of low dissolved oxy...

journal_title：Applied microbiology and biotechnology

authors： Heyen U,Schüler D

更新日期：2003-06-01 00:00:00

abstract：:The binaphthyl derivative cladosporol 3 was supplied from 60 to 200 mg l(-1) to shaken cultures of Cladosporium cf. cladosporioides. Compared to blank, fungal biomass was not affected by adding cladosporol till 100 mg l(-1): it rather increased at higher ratios between 150 and 200 mg l(-1). The production of the major...

journal_title：Applied microbiology and biotechnology

authors： Assante G,Bava A,Nasini G

更新日期：2006-02-01 00:00:00

abstract：:D-lactic acid is used as a monomer in the production of poly-D-lactic acid (PDLA), which is used to form heat-resistant stereocomplex poly-lactic acid. To produce cost-effective D-lactic acid by using all sugars derived from biomass efficiently, xylose-assimilating genes encoding xylose isomerase and xylulokinase were...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Vadlani PV,Kumar A,Hardwidge PR,Govind R,Tanaka T,Kondo A

更新日期：2016-01-01 00:00:00

abstract：:Fidaxomicin, an 18-membered macrolide antibiotic, is highly active against Clostridium difficile, the most common cause of diarrhea in hospitalized patients. Though the biosynthetic mechanism of fidaxomicin has been well studied, little is known about its regulatory mechanism. Here, we reported that FadR1, a LAL famil...

journal_title：Applied microbiology and biotechnology

authors： Li YP,Yu P,Li JF,Tang YL,Bu QT,Mao XM,Li YQ

更新日期：2019-09-01 00:00:00

abstract：:Mixed viral infections are common in fields and frequently exacerbate disease severity via synergistic interactions among individual viral genomic components leading to major crop loss. Two predominant species of tomato-infecting begomoviruses, Tomato leaf curl New Delhi virus (ToLCNDV) and Tomato leaf curl Gujarat vi...

journal_title：Applied microbiology and biotechnology

authors： Basu S,Singh AK,Singh D,Sahu SK,Chakraborty S

更新日期：2021-01-08 00:00:00

abstract：:The secreted proteome of Pichia pastoris X-33 was investigated in methanol-induced cultures with a goal to enhance the secretion and purification of recombinant proteins. In a fed-batch fermentation at 30 °C, more host proteins were found in greater concentrations compared to cultures grown at 25 °C. Protein samples c...

journal_title：Applied microbiology and biotechnology

authors： Huang CJ,Damasceno LM,Anderson KA,Zhang S,Old LJ,Batt CA

更新日期：2011-04-01 00:00:00

abstract：:The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes...

journal_title：Applied microbiology and biotechnology

authors： Jin FJ,Han P,Zhuang M,Zhang ZM,Jin L,Koyama Y

更新日期：2018-01-01 00:00:00

abstract：:The Baeyer-Villiger monooxygenase (BVMO) BmoF1 from Pseudomonas fluorescens DSM 50106 was shown before to enantioselectively oxidize different 4-hydroxy-2-ketones to the corresponding hydroxyalkyl acetates, being the first example of a BVMO-catalyzed kinetic resolution of aliphatic acyclic ketones. However, the wild-t...

journal_title：Applied microbiology and biotechnology

authors： Kirschner A,Bornscheuer UT

更新日期：2008-12-01 00:00:00

abstract：:Problems with deflocculation and solids separation in biological wastewater treatment systems are linked to fluctuations in physicochemical conditions. This study examined the composition of activated sludge bacterial communities in lab-scale sequencing batch reactors treating bleached kraft mill effluent, under trans...

journal_title：Applied microbiology and biotechnology

authors： Nadarajah N,Allen DG,Fulthorpe RR

更新日期：2010-11-01 00:00:00

abstract：:The principal point of this paper is that the discovery of penicillin and the development of the supporting technologies in microbiology and chemical engineering leading to its commercial scale production represent it as the medicine with the greatest impact on therapeutic outcomes. Our nomination of penicillin for th...

journal_title：Applied microbiology and biotechnology

authors： Kardos N,Demain AL

更新日期：2011-11-01 00:00:00

abstract：:Sulfadiazine (SDZ) is an antibiotic frequently administered to livestock, and it alters microbial communities when entering soils with animal manure, but understanding the interactions of these effects to the prevailing climatic regime has eluded researchers. A climatic factor that strongly controls microbial activity...

journal_title：Applied microbiology and biotechnology

authors： Reichel R,Radl V,Rosendahl I,Albert A,Amelung W,Schloter M,Thiele-Bruhn S

更新日期：2014-01-01 00:00:00

abstract：:Both varied and strong promoters are essential for metabolic and pathway engineering applications in any host organism. To enable this capacity, here we demonstrate a generalizable method for the de novo construction of strong, synthetic hybrid promoter libraries. Specifically, we demonstrate how promoter truncation a...

journal_title：Applied microbiology and biotechnology

authors： Blazeck J,Reed B,Garg R,Gerstner R,Pan A,Agarwala V,Alper HS

更新日期：2013-04-01 00:00:00

abstract：:The addition of humic acid (HA) to polycyclic aromatic hydrocarbon (PAH) contaminated systems has been shown to enhance, inhibit, or have no effect on the biodegradation of these PAHs. In this study, the surfactant effects of Elliott soil HA (ESHA) at two pH values were tested. At pH 7.0, ESHA did not behave as a surf...

journal_title：Applied microbiology and biotechnology

authors： Liang Y,Britt DW,McLean JE,Sorensen DL,Sims RC

更新日期：2007-04-01 00:00:00

abstract：:Lipase has been used widely in industry. In this study, we have constructed two recombinant Saccharomyces cerevisiae strains displaying two active lipases on the cell surface by cell surface engineering. The genes encoding Yarrowia lipolytica lipases Lip7 and Lip8 were fused with the gene encoding small binding subuni...

journal_title：Applied microbiology and biotechnology

authors： Liu WS,Pan XX,Jia B,Zhao HY,Xu L,Liu Y,Yan YJ

更新日期：2010-10-01 00:00:00

abstract：:Biocatalytic asymmetric preparation of (R)-1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent (R)-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on m...

journal_title：Applied microbiology and biotechnology

authors： Zheng RC,Ge Z,Qiu ZK,Wang YS,Zheng YG

更新日期：2012-05-01 00:00:00

abstract：:Four types of cell-surface-engineered yeast Saccharomyces cerevisiae displaying glucoamylase, namely, systems A, B, C, and D, were constructed to evaluate their performance in direct ethanol fermentation from raw corn starch. Systems A and B were glucoamylase-displaying nonflocculent yeast (YF237) types that secrete a...

journal_title：Applied microbiology and biotechnology

authors： Khaw TS,Katakura Y,Koh J,Kondo A,Ueda M,Shioya S

更新日期：2006-05-01 00:00:00