Establishment of a functional secretory IgA transcytosis model system in vitro for functional food screening.

abstract：:Microbial transformation of benzaldehyde into L: -phenylacetylcarbinol by whole cell Saccharomyces cerevisiae has been carried out in a novel polyethylene glycol (PEG)-induced cloud point system. The system is composed of 80 g PEG 20,000, 75 ml Triton X-100, 20 g peptone, 10 g yeast extract, 25 g glucose, 1 g MgSO(4)....

journal_title：Applied microbiology and biotechnology

authors： Zhang W,Wang Z,Li W,Zhuang B,Qi H

更新日期：2008-02-01 00:00:00

abstract：:We report an immuno-magnetic separation system developed by the immobilization of pathogen-specific antibodies on the core-shell magnetic beads. The magnetic beads were grafted with glycidylmethacrylate (GMA) using surface-initiated atom transfer radical polymerization (SI-ATRP). For immuno-magnetic separation (IMS) o...

journal_title：Applied microbiology and biotechnology

authors： Ozalp VC,Bayramoglu G,Arica MY,Oktem HA

更新日期：2013-11-01 00:00:00

abstract：:"Milking processing" describes the cultivation of microalgae in a water-organic solvent two-phase system that consists of simultaneous fermentation and secretion of intracellular product. It is usually limited by the conflict between the biocompatibility of the organic solvent to the microorganisms and the ability of ...

journal_title：Applied microbiology and biotechnology

authors： Hu Z,Zhang X,Wu Z,Qi H,Wang Z

更新日期：2012-04-01 00:00:00

abstract：:A novel and efficient screening method for pyrroloquinoline quinone (PQQ) high-yielding methylotrophic strains was developed by using glucose dehydrogenase apoenzyme (GDHA) which depended on PQQ as the cofactor. Using this high-throughput method, PQQ high-yielding strains were rapidly screened out from thousands of me...

journal_title：Applied microbiology and biotechnology

authors： Si Z,Zhu J,Wang W,Huang L,Wei P,Cai J,Xu Z

更新日期：2016-12-01 00:00:00

abstract：:Nonribosomal peptides (NRPs), a large family of natural products, possess numerous pharmaceutically significant bioactivities. However, many native microbial producers of NRPs are not cultivable or have low production yields making mass production infeasible. The recombinant production of natural products in a surroga...

journal_title：Applied microbiology and biotechnology

authors： Li J,Jaitzig J,Hillig F,Süssmuth R,Neubauer P

更新日期：2014-01-01 00:00:00

abstract：:Ene-reductases originating from extremophiles are gaining importance in the field of biocatalysis due to higher-stability properties. The genome of the acidophilic iron-oxidizing bacterium "Ferrovum" sp. JA12 was found to harbor a thermophilic-like ene-reductase (FOYE-1). The foye-1 gene was ligated into a pET16bp exp...

journal_title：Applied microbiology and biotechnology

authors： Scholtissek A,Ullrich SR,Mühling M,Schlömann M,Paul CE,Tischler D

更新日期：2017-01-01 00:00:00

abstract：:Stable transformants of Chromobacterium violaceum were obtained by high-voltage electroporation with a 7-kilobase binary plasmid. The technique was reliable, reproducible, and simple, with efficiencies of 10(5) transformants/microg of plasmid DNA. The electrical conditions that resulted in the highest efficiencies wer...

journal_title：Applied microbiology and biotechnology

authors： Broetto L,Cecagno R,Sant'anna FH,Weber S,Schrank IS

更新日期：2006-07-01 00:00:00

abstract：:Rhodococcus erythropolis U23A is a polychlorinated biphenyl (PCB)-degrading bacterium isolated from the rhizosphere of plants grown on a PCB-contaminated soil. Strain U23A bphA exhibited 99% identity with bphA1 of Rhodococcus globerulus P6. We grew Arabidopsis thaliana in a hydroponic axenic system, collected, and con...

journal_title：Applied microbiology and biotechnology

authors： Toussaint JP,Pham TT,Barriault D,Sylvestre M

更新日期：2012-09-01 00:00:00

abstract：:The application of biocontrol biopesticides based on plant growth-promoting rhizobacteria (PGPR), particularly members of the genus Bacillus, is considered a promising perspective to make agricultural practices sustainable and ecologically safe. Recent advances in genome sequencing by third-generation sequencing techn...

journal_title：Applied microbiology and biotechnology

authors： Reva ON,Larisa SA,Mwakilili AD,Tibuhwa D,Lyantagaye S,Chan WY,Lutz S,Ahrens CH,Vater J,Borriss R

更新日期：2020-09-01 00:00:00

abstract：:Pseudomonas aeruginosa and Staphylococcus aureus are versatile bacterial pathogens and common etiological agents in polymicrobial infections. Microbial communities containing both of these pathogens are shaped by interactions ranging from parasitic to mutualistic, with the net impact of these interactions in many case...

journal_title：Applied microbiology and biotechnology

authors： Nguyen AT,Oglesby-Sherrouse AG

更新日期：2016-07-01 00:00:00

abstract：:The marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental c...

journal_title：Applied microbiology and biotechnology

authors： Kautharapu KB,Rathmacher J,Jarboe LR

更新日期：2013-04-01 00:00:00

abstract：:In natural settings, anaerobic digestion can take place in a wide temperature range, but industrial digesters are usually operated under either mesophilic (~35 °C) or thermophilic (~55 °C) conditions. The ability of anaerobic digestion microbiota to switch from one operating temperature to the other remains poorly doc...

journal_title：Applied microbiology and biotechnology

authors： Chapleur O,Mazeas L,Godon JJ,Bouchez T

更新日期：2016-02-01 00:00:00

abstract：:Magnetotactic bacteria are difficult to grow under defined conditions in culture, which has presented a major obstacle to commercial application of magnetosomes. We studied the relationships among the cell growth, magnetosome formation, dissolved oxygen concentration (DO), and the ability to supply oxygen to the cells...

journal_title：Applied microbiology and biotechnology

authors： Sun JB,Zhao F,Tang T,Jiang W,Tian JS,Li Y,Li JL

更新日期：2008-06-01 00:00:00

abstract：:A Gram-negative anaerobic microorganism, MRG-1, isolated from human intestine showed high activities of deglycosylation and reduction of daidzin, based on rapid TLC analysis. A rod-shaped strain MRG-1 was identified as a new species showing 91.0% homology to Coprobacillus species, based on 16S rRNA sequence analysis. ...

journal_title：Applied microbiology and biotechnology

authors： Park HY,Kim M,Han J

更新日期：2011-08-01 00:00:00

abstract：:Clostridium tyrobutyricum is a promising organism for butyrate and n-butanol production, but cannot grow on sucrose. Three genes (scrA, scrB, and scrK) involved in the sucrose catabolic pathway, along with an aldehyde/alcohol dehydrogenase gene, were cloned from Clostridium acetobutylicum and introduced into C. tyrobu...

journal_title：Applied microbiology and biotechnology

authors： Zhang J,Yu L,Xu M,Yang ST,Yan Q,Lin M,Tang IC

更新日期：2017-05-01 00:00:00

abstract：:Autotransporters (ATs) are associated with pathogenesis of Avian Pathogenic Escherichia coli (APEC). The molecular characterization of APEC ATs can provide insights about their relevance to APEC pathogenesis. Here, we characterized a conventional autotransporter UpaB in APEC DE205B genome. The upaB existed in 41.9 % o...

journal_title：Applied microbiology and biotechnology

authors： Zhu-Ge XK,Pan ZH,Tang F,Mao X,Hu L,Wang SH,Xu B,Lu CP,Fan HJ,Dai JJ

更新日期：2015-12-01 00:00:00

abstract：:We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region ...

journal_title：Applied microbiology and biotechnology

authors： Piret J,Resendiz B,Mahro B,Zhang JY,Serpe E,Romero J,Connors N,Demain AL

更新日期：1990-02-01 00:00:00

abstract：:Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A s...

journal_title：Applied microbiology and biotechnology

authors： Morikawa M,Daido H,Pongpobpibool S,Imanaka T

更新日期：1994-11-01 00:00:00

abstract：:Pseudomonas sp. D7-4 and Pseudomonas sp. B13 FR1(pFRC20P) degraded mixtures of chloro- and methyl-substituted benzoates exclusively via an extended ortho pathway, whereas in Pseudomonas putida WR 201 both ortho and meta fission were induced by mixtures of 3-chloro- and 3-methylbenzoate or even by 3-chloro-benzoate alo...

journal_title：Applied microbiology and biotechnology

authors： Franck-Mokross AC,Schmidt E

更新日期：1998-08-01 00:00:00

abstract：:Drug-resistant bacteria are becoming an increasingly widespread problem in the clinical setting. The current pipeline of antibiotics cannot provide satisfactory options for clinicians, which brought increasing attention to the development and application of non-traditional antimicrobial substances as alternatives. Met...

journal_title：Applied microbiology and biotechnology

authors： Ye Q,Chen W,Huang H,Tang Y,Wang W,Meng F,Wang H,Zheng Y

更新日期：2020-06-01 00:00:00

abstract：:Five types of pharmaceuticals and personal care products (PPCPs) substances were selected as pollutants in this study. The effects of the removal of these pollutants and the microbial succession process in a granular sludge membrane bioreactor (GMBR) were investigated. Results showed that wastewater containing PPCPs i...

journal_title：Applied microbiology and biotechnology

authors： Wang XC,Shen JM,Chen ZL,Zhao X,Xu H

更新日期：2016-09-01 00:00:00

abstract：:Some strains belonging to the genera Citrobacter and Enterobacter have been reported to produce chitin/chitosan-like bioflocculants (BFs) from acetate. In this study, to investigate the distribution of the BF-producing potential in the genus Citrobacter and to screen stably and highly BF-producing strains, we obtained...

journal_title：Applied microbiology and biotechnology

authors： Kimura K,Inoue T,Kato D,Negoro S,Ike M,Takeo M

更新日期：2013-11-01 00:00:00

abstract：:C (2) to C (4) chloroalkanes have been used for a wide range of industrial applications. Consequently, numerous leaks to the environment have occurred. It is generally observed that the lower chlorinated members of the group, containing 1-3 chlorine atoms, accumulate in environments where reductive conditions prevail....

journal_title：Applied microbiology and biotechnology

authors： De Wildeman S,Verstraete W

更新日期：2003-04-01 00:00:00

abstract：:Aflatoxins are toxic and carcinogenic metabolites of several Aspergillus species. The effect of nitrate on aflatoxin production and expression of the key regulatory genes involved in aflatoxin biosynthesis, aflR and aflJ, were compared among isolates of the S(B) and S(BG) strains of A. flavus. Aflatoxin production by ...

journal_title：Applied microbiology and biotechnology

authors： Ehrlich KC,Cotty PJ

更新日期：2002-10-01 00:00:00

abstract：:The Vitreoscilla hemoglobin (VHb) gene (vgb) was integrated into the chromosome of Bacillus thuringiensis BMB171 using integrative vector pEG491. The production of VHb was confirmed by CO-difference spectra analysis. Fermentation experiments results showed that with the production of VHb, the critical oxygen concentra...

journal_title：Applied microbiology and biotechnology

authors： Liang F,Shouwen C,Ming S,Ziniu Y

更新日期：2007-02-01 00:00:00

abstract：:Sustainable tea cultivation will rely increasingly on alternatives to conventional chemical insecticides for pest management that are environment-friendly and reduce the amount of pesticide residues in made tea. Entomopathogens can provide effective control, conserve biodiversity, and serve as alternatives to chemical...

journal_title：Applied microbiology and biotechnology

authors： Roy S,Muraleedharan N

更新日期：2014-06-01 00:00:00

abstract：:Cyanobacterial blooms have become a serious problem in Lake Taihu during the last 20 years, and Microcystis aeruginosa and Synechococcus sp. are the two dominant species in cyanobacterial blooms of Lake Taihu. A freshwater bacterial strain, Shewanella sp. Lzh-2, with strong algicidal properties against harmful cyanoba...

journal_title：Applied microbiology and biotechnology

authors： Li Z,Lin S,Liu X,Tan J,Pan J,Yang H

更新日期：2014-05-01 00:00:00

abstract：:The desire to reduce dependence on the ever diminishing fossil fuel reserves coupled with the impetus towards green energy has seen increased research in biofuels as alternative sources of energy. Lignocellulose materials are one of the most promising feedstocks for advanced biofuels production. However, their utilisa...

journal_title：Applied microbiology and biotechnology

authors： Kudanga T,Le Roes-Hill M

更新日期：2014-08-01 00:00:00

abstract：:In this study, we demonstrated that anaerobic granular sludge could be successfully transformed into aerobic granular sludge in a continuous up-flow reactor in 45 days. An aerobic microbial community successfully developed in the granules and high organic matter and nitrogen removal performance was achieved. Under an ...

journal_title：Applied microbiology and biotechnology

authors： Sun H,Yu P,Li Q,Ren H,Liu B,Ye L,Zhang XX

更新日期：2017-10-01 00:00:00

abstract：:The white button mushroom Agaricus bisporus is economically the most important commercially produced edible fungus. It is grown on carbon- and nitrogen-rich substrates, such as composted cereal straw and animal manure. The commercial mushroom production process is usually performed in buildings or tunnels under highly...

journal_title：Applied microbiology and biotechnology

authors： Kabel MA,Jurak E,Mäkelä MR,de Vries RP

更新日期：2017-06-01 00:00:00