A thermophilic-like ene-reductase originating from an acidophilic iron oxidizer.

abstract：:Ribonuclease production using immobilized cells (IC) of Aspergillus clavatus has been studied under batch, repeated-batch and continuous fermentation conditions in a bubble-column bioreactor and compared with production by free cells. Immobilization was achieved by the method of cryostructurization in polyvinyl alcoho...

journal_title：Applied microbiology and biotechnology

authors： Manolov RJ

更新日期：1992-04-01 00:00:00

abstract：:Enzymatic kinetic resolution is a widely used biotechnological tool for the production of enantiomerically pure/enriched compounds. This technique takes advantage of the enantioselectivity or enantiospecificity of an enzyme for one of the enantiomers of a racemic substrate to isolate the desired isomer. N-Carbamoyl-D-...

journal_title：Applied microbiology and biotechnology

authors： Martínez-Rodríguez S,Martínez-Gómez AI,Rodríguez-Vico F,Clemente-Jiménez JM,Las Heras-Vázquez FJ

更新日期：2010-01-01 00:00:00

abstract：:Avian influenza virus (AIV) is spreading worldwide and is a serious threat to the health of poultry and humans. In many countries, low pathogenic AIVs, such as H9N2, have become an enormous economic burden on the commercial poultry industry because they cause mild respiratory disease and decrease egg production. A rec...

journal_title：Applied microbiology and biotechnology

authors： Yang WT,Yang GL,Shi SH,Liu YY,Huang HB,Jiang YL,Wang JZ,Shi CW,Jing YB,Wang CF

更新日期：2017-06-01 00:00:00

abstract：:Chlorogenate hydrolase (EC 3.1.1.42, CHase) was highly induced in mycelia of Aspergillus sojae AKU 3312 grown in Czapek medium containing either instant coffee powder or coffee pulp as inducer. No CHase formation was observed in the mycelia when cultivated without the inducer. CHase was purified readily from CHase-ind...

journal_title：Applied microbiology and biotechnology

authors： Adachi O,Ano Y,Akakabe Y,Shinagawa E,Matsushita K

更新日期：2008-11-01 00:00:00

abstract：:Using a reverse-transcription polymerase chain reaction (RT-PCR) technique, cytochrome P450 genes were cloned from the lignin-degrading basidiomycete, Coriolus versicolor. One possible P450 gene was identified, which consisted of 1,672 nucleotides and a poly(A) tail and encoded a deduced protein containing 449 amino a...

journal_title：Applied microbiology and biotechnology

authors： Ichinose H,Wariishi H,Tanaka H

更新日期：2002-01-01 00:00:00

abstract：:Overuse of broad-spectrum antibiotics to control human and plant pathogens greatly accelerated the development of antibiotic resistance among bacteria and fungi. Therefore, usage of new approaches is necessary to control outbreaks of phytopathogenic diseases as well as multidrug-resistant human pathogens. Many of the ...

journal_title：Applied microbiology and biotechnology

authors： Olishevska S,Nickzad A,Déziel E

更新日期：2019-02-01 00:00:00

abstract：:Streptomyces lividans IAF18, obtained by homologous cloning, is capable of over-producing XlnA. To investigate the possibility of the expression of foreign genes, various coding regions of the xylanase A gene (xlnA) were analysed. Expression/secretion vectors were constructed containing the regulatory elements of xlnA...

journal_title：Applied microbiology and biotechnology

authors： Paradis FW,Shareck F,Dupont C,Kluepfel D,Morosoli R

更新日期：1996-06-01 00:00:00

abstract：:Biotin (Vitamin B7) is a critical enzyme co-factor in metabolic pathways important for bacterial survival. Biotin is obtained either from the environment or by de novo synthesis, with some bacteria capable of both. In certain species, the bifunctional protein BirA plays a key role in biotin homeostasis as it regulates...

journal_title：Applied microbiology and biotechnology

authors： Satiaputra J,Eijkelkamp BA,McDevitt CA,Shearwin KE,Booker GW,Polyak SW

更新日期：2018-04-01 00:00:00

abstract：:The quorum sensing signalling molecules 2-heptyl-3-hydroxy-4(1H)-quinolone, termed the "Pseudomonas quinolone signal" (PQS), and 2-heptyl-4(1H)-quinolone (HHQ) play an important role in the control of virulence gene expression in Pseudomonas aeruginosa. To construct a bioreporter for the specific and sensitive detecti...

journal_title：Applied microbiology and biotechnology

authors： Müller C,Fetzner S

更新日期：2013-01-01 00:00:00

abstract：:Thirty-four strains of lactic acid bacteria (seven Bifidobacterium, 11 Lactobacillus, six Lactococcus, and 10 Streptococcus thermophilus) were assayed in vitro for antioxidant activity against ascorbic and linolenic acid oxidation (TAA(AA) and TAA(LA)), trolox-equivalent antioxidant capacity (TEAC), intracellular glut...

journal_title：Applied microbiology and biotechnology

authors： Amaretti A,di Nunzio M,Pompei A,Raimondi S,Rossi M,Bordoni A

更新日期：2013-01-01 00:00:00

abstract：:Nanotechnology is a promising field of interdisciplinary research. It opens up a wide array of opportunities in various fields like medicine, pharmaceuticals, electronics and agriculture. The potential uses and benefits of nanotechnology are enormous. These include insect pests management through the formulations of n...

journal_title：Applied microbiology and biotechnology

authors： Rai M,Ingle A

更新日期：2012-04-01 00:00:00

abstract：:Because engineering of the 101.016-bp megaplasmid pKB1 of Gordonia westfalica Kb1 failed due to the absence of an effective transfer system, pKB1 was transferred by conjugation from G. westfalica Kb1 to a kanamycin-resistant mutant of Rhodococcus opacus PD630 at a frequency of about 6.2 x 10(-8) events per recipient c...

journal_title：Applied microbiology and biotechnology

authors： Bröker D,Arenskötter M,Steinbüchel A

更新日期：2008-01-01 00:00:00

abstract：:The biochemical oxygen demand (BOD) value is still a key parameter that can determine the level of organics, particularly the content of biodegradable organics in water. In this work, the effects of sample dilution, which should be done inevitably to get appropriate dissolved oxygen (DO) depletion, on the measurement ...

journal_title：Applied microbiology and biotechnology

authors： Seo KS,Chang HN,Park JK,Choo KH

更新日期：2007-09-01 00:00:00

abstract：:The gene ce1A, encoding an endoglucanase from the strain Bacillus sp. BP-23, was cloned and expressed in Escherichia coli. The nucleotide sequence of a 1867-bp DNA fragment containing the ce1A gene was determined, revealing an open reading frame of 1200 nucleotides that encodes a protein of 44,803 Da. The deduced amin...

journal_title：Applied microbiology and biotechnology

authors： Blanco A,Díaz P,Martínez J,Vidal T,Torres AL,Pastor FI

更新日期：1998-07-01 00:00:00

abstract：:By damaging DNA molecules, genotoxicants cause genetic mutations and also increase human susceptibility to cancers and genetic diseases. Over the past four decades, several assays have been developed in the budding yeast Saccharomyces cerevisiae to screen potential genotoxic substances and provide alternatives to anim...

journal_title：Applied microbiology and biotechnology

authors： Eki T

更新日期：2018-03-01 00:00:00

abstract：:The denitrifier community and associated nitrate and nitrite reduction in the bioaugmented and general sequencing batch reactors (SBRs) during the treatment of coking wastewater containing pyridine and quinoline were investigated. The efficiency and stability of nitrate and nitrite reduction in SBR was considerably im...

journal_title：Applied microbiology and biotechnology

authors： Bai Y,Sun Q,Xing R,Wen D,Tang X

更新日期：2011-05-01 00:00:00

abstract：:A gratuitous strain was developed by disrupting the GAL1 gene (galactokinase) of recombinant Saccharomyces cerevisiae harboring the antithrombotic hirudin gene in the chromosome under the control of the GAL10 promoter. A series of glucose-limited fed-batch cultures were carried out to examine the effects of glucose su...

journal_title：Applied microbiology and biotechnology

authors： Kim MD,Lee TH,Lim HK,Seo JH

更新日期：2004-08-01 00:00:00

abstract：:The araA gene encoding an L-arabinose isomerase (L-AI) from the acido-thermophilic bacterium Acidothermus cellulolytics ATCC 43068 was cloned and overexpressed in Escherichia coli. The open reading frame of the L-AI consisted of 1,503 nucleotides encoding 501 amino acid residues. The recombinant L-AI was purified to h...

journal_title：Applied microbiology and biotechnology

authors： Cheng L,Mu W,Zhang T,Jiang B

更新日期：2010-04-01 00:00:00

abstract：:To provide new insight into the dark fermentation process, a multi-lateral study was performed to study the microbiology of 20 different lab-scale bioreactors operated in four different countries (Brazil, Chile, Mexico, and Uruguay). Samples (29) were collected from bioreactors with different configurations, operation...

journal_title：Applied microbiology and biotechnology

authors： Etchebehere C,Castelló E,Wenzel J,del Pilar Anzola-Rojas M,Borzacconi L,Buitrón G,Cabrol L,Carminato VM,Carrillo-Reyes J,Cisneros-Pérez C,Fuentes L,Moreno-Andrade I,Razo-Flores E,Filippi GR,Tapia-Venegas E,Toledo-Alarcón J

更新日期：2016-04-01 00:00:00

abstract：:Linkages among bioreactor operation and performance and microbial community structure were investigated for a fixed-bed biofilm system designed to remove perchlorate from drinking water. Perchlorate removal was monitored to evaluate reactor performance during and after the frequency and intensity of the backwash proce...

journal_title：Applied microbiology and biotechnology

authors： Li X,Yuen W,Morgenroth E,Raskin L

更新日期：2012-11-01 00:00:00

abstract：:A set of mutants was generated by targeted deletion of the hsdR loci of two type I restriction modification systems (RMS) identified in Bacillus licheniformis DSM13. Single as well as double knock-outs resulted in strains being readily transformable with plasmids isolated from Bacilli. Introduction of shuttle plasmids...

journal_title：Applied microbiology and biotechnology

authors： Waschkau B,Waldeck J,Wieland S,Eichstädt R,Meinhardt F

更新日期：2008-02-01 00:00:00

abstract：:Transfer of a biosynthetic pathway between evolutionary distant organisms can create a metabolic shunt capable of bypassing the native regulation of the host organism, hereby improving the production of secondary metabolite precursor molecules for important natural products. Here, we report the engineering of Escheric...

journal_title：Applied microbiology and biotechnology

authors： Carlsen S,Ajikumar PK,Formenti LR,Zhou K,Phon TH,Nielsen ML,Lantz AE,Kielland-Brandt MC,Stephanopoulos G

更新日期：2013-07-01 00:00:00

abstract：:In this study, we employed TiO₂ enrichment and high accuracy liquid chromatography-mass spectrometry-mass spectrometry to identify the phosphoproteome of Clostridium acetobutyicum ATCC824 in acidogenesis and solventogenesis. As many as 82 phosphopeptides in 61 proteins, with 107 phosphorylated sites on serine, threoni...

journal_title：Applied microbiology and biotechnology

authors： Bai X,Ji Z

更新日期：2012-07-01 00:00:00

abstract：:Aerobic granules are cultivated by a single bacterial strain, Acinetobacter calcoaceticus, in a sequencing batch reactor (SBR). This strain presents as a good phenol reducer and an efficient auto coagulator in the presence of phenol, mediated by heat-sensitive adhesins proteins. Stable 2.3-mm granules were formed in t...

journal_title：Applied microbiology and biotechnology

authors： Adav SS,Lee DJ

更新日期：2008-03-01 00:00:00

abstract：:Aspergillus oryzae is a fungus used extensively in the fermentation industry. We constructed cDNA microarrays comprising 2,070 highly expressed cDNAs selected from the approximately 6,000 non-redundant expressed sequence tags (ESTs) in the A. oryzae EST database (http://www.aist.go.jp/RIODB/ffdb/index.html). Using the...

journal_title：Applied microbiology and biotechnology

authors： Maeda H,Sano M,Maruyama Y,Tanno T,Akao T,Totsuka Y,Endo M,Sakurada R,Yamagata Y,Machida M,Akita O,Hasegawa F,Abe K,Gomi K,Nakajima T,Iguchi Y

更新日期：2004-07-01 00:00:00

abstract：:Sophorolipids are surface-active compounds synthesized by a selected number of yeast species. They have been known for over 40 years, but because of growing environmental awareness, they recently regained attention as biosurfactants due to their biodegradability, low ecotoxicity, and production based on renewable reso...

journal_title：Applied microbiology and biotechnology

authors： Van Bogaert IN,Saerens K,De Muynck C,Develter D,Soetaert W,Vandamme EJ

更新日期：2007-08-01 00:00:00

abstract：:The Gram-positive Corynebacterium glutamicum is widely used for fermentative production of amino acids. The world production of L-lysine has surpassed 2 million tons per year. Glucose uptake and phosphorylation by C. glutamicum mainly occur by the phosphotransferase system (PTS) and to lesser extent by inositol permea...

journal_title：Applied microbiology and biotechnology

authors： Pérez-García F,Peters-Wendisch P,Wendisch VF

更新日期：2016-09-01 00:00:00

abstract：:Twenty-six species of Phellinus sensu lato, reported as medicinal mushrooms, are enumerated in this review. The species' names were checked and revised according to contemporary taxonomy and the latest version of the International Code of Botanical Nomenclature (Vienna Code); two misapplied names of Phellinus baumii P...

journal_title：Applied microbiology and biotechnology

authors： Dai YC,Zhou LW,Cui BK,Chen YQ,Decock C

更新日期：2010-08-01 00:00:00

abstract：:Transformants of the Trichoderma reeseistrains QM9414 and Rut-C30 were constructed in which the genes for the two major hydrophobin proteins, hydrophobins I (HFBI) and II (HFBII), were deleted or amplified by molecular biological techniques. Growth parameters and foam production of the transformant strains were compar...

journal_title：Applied microbiology and biotechnology

authors： Bailey MJ,Askolin S,Hörhammer N,Tenkanen M,Linder M,Penttilä M,Nakari-Setälä T

更新日期：2002-05-01 00:00:00

abstract：:Real-time polymerase chain reaction (RT-PCR) was used to quantify seven species of lactic acid bacteria (LAB) in alfalfa silage prepared in the presence or absence of four commercial inoculants and in uninoculated corn stover harvested and stored under a variety of field conditions. Species-specific PCR primers were d...

journal_title：Applied microbiology and biotechnology

authors： Stevenson DM,Muck RE,Shinners KJ,Weimer PJ

更新日期：2006-07-01 00:00:00