The effects of upaB deletion and the double/triple deletion of upaB, aatA, and aatB genes on pathogenicity of avian pathogenic Escherichia coli.

abstract：:Calcineurin is highly conserved and regulates growth, conidiation, stress response, and pathogenicity in fungi. However, the functions of calcineurin and its regulatory network in entomopathogenic fungi are not clear. In this study, calcineurin was functionally analyzed by deleting the catalytic subunit MaCnA from the...

journal_title：Applied microbiology and biotechnology

authors： Cao Y,Du M,Luo S,Xia Y

更新日期：2014-10-01 00:00:00

abstract：:The TM1072 gene from Thermotoga maritima codifies for a putative form of a rhamnulose-1-phosphate aldolase (Rha-1PA Tm). To investigate this enzyme further, its gene was cloned and expressed in Escherichia coli. The purified enzyme was activated by Co(2+) as a divalent metal ion cofactor, instead of Zn(2+) as its E. c...

journal_title：Applied microbiology and biotechnology

authors： Oroz-Guinea I,Sánchez-Moreno I,Mena M,García-Junceda E

更新日期：2015-04-01 00:00:00

abstract：:Heterologous biosynthesis of tetrahydrocannabinolic acid (THCA) in yeast is a biotechnological process in Natural Product Biotechnology that was recently introduced. Based on heterologous genes from Cannabis sativa and Streptomyces spp. cloned into Saccharomyces cerevisiae, the heterologous biosynthesis was fully embe...

journal_title：Applied microbiology and biotechnology

authors： Thomas F,Schmidt C,Kayser O

更新日期：2020-11-01 00:00:00

abstract：:The effects of copper (Cu), chromium (Cr), cadmium (Cd), lead (Pb) and zinc (Zn) on the biotransformation of organic acids (acetate, propionate and butyrate) and H2 were assessed in serum-bottle microcosms. Experiments were performed over a range of metal concentrations (20-200 mg/l) using biomass from an anaerobic bi...

journal_title：Applied microbiology and biotechnology

authors： Kong IC,Hubbard JS,Jones WJ

更新日期：1994-11-01 00:00:00

abstract：:Photosynthetic microorganisms produce relatively large amounts of physiologically active materials which stimulate the physiological activity of other organisms. In this study, mammalian HeLa cells were cultured in different culture media which were Dulbecco's modified Eagle medium (DMEM) with newborn calf serum (NCS)...

journal_title：Applied microbiology and biotechnology

authors： Lee HJ,Park JY,Yoo KS,Yoon J,Kim YH,Min J

更新日期：2013-11-01 00:00:00

abstract：:Interleukin-2 (IL-2) is one of the most successful cytokines applied in tumor immunotherapy because of its ability to stimulate potent cellular immune response. The life-threatening toxicity of vascular leak syndrome (VLS) associated with the high-dose IL-2 treatment regimen has limited its use in tumor immunotherapy....

journal_title：Applied microbiology and biotechnology

authors： Ye L,Fan J,Shi X,Tao Q,Ye D,Xian Z,Zeng X,Li Y,Feng M,Ju D

更新日期：2014-05-01 00:00:00

abstract：:The nucleoprotein of infectious hematopoietic necrosis virus (IHNV) is considered as the main target antigen for detection of IHNV infection in salmonid fish. This study aimed at improving the expression and solubility of IHNV nucleoprotein (IHNV-NP) in E. coli expression system. The effects of several expression stra...

journal_title：Applied microbiology and biotechnology

authors： Mohammadinezhad R,Farahmand H,Jalali SAH,Mirvaghefi A

更新日期：2018-05-01 00:00:00

abstract：:The genus Aspergillus is widely used for the production of plant cell wall polysaccharide-degrading enzymes. The range of enzymes purified from these fungi covers nearly every function required for the complete degradation of cellulose, xyloglucan, xylan, galacto(gluco)mannan and pectin. This paper describes the Asper...

journal_title：Applied microbiology and biotechnology

authors： de Vries RP

更新日期：2003-03-01 00:00:00

abstract：:Taxol is an anticancer identified in both endophytic fungus and its host plant. Plant Taxol is a diterpenoid with geranylgeranyl diphosphate (GGPP) mediates the biosynthesis of its terpenoid moiety. Previous report has suggested that fungal Taxol may require terpenoid pathway for its biosynthesis. Here in this study, ...

journal_title：Applied microbiology and biotechnology

authors： Soliman SSM,Mosa KA,El-Keblawy AA,Husseiny MI

更新日期：2017-10-01 00:00:00

abstract：:The microbial production of biofuels is a promising avenue for the development of viable processes for the generation of fuels from sustainable resources. In order to become cost and energy effective, these processes must utilize organisms that can be optimized to efficiently produce candidate fuels from a variety of ...

journal_title：Applied microbiology and biotechnology

authors： Clomburg JM,Gonzalez R

更新日期：2010-03-01 00:00:00

abstract：:Two putative chitinase genes, lin0153 and lin1996, from the nonpathogenic bacterium Listeria innocua were expressed in Escherichia coli, and the gene products were characterized. The genes were close homologs of chitinases from the pathogenic bacterium Listeria monocytogenes, in which chitinases and chitin-binding pro...

journal_title：Applied microbiology and biotechnology

authors： Honda S,Wakita S,Sugahara Y,Kawakita M,Oyama F,Sakaguchi M

更新日期：2016-09-01 00:00:00

abstract：:The intricate patterning of diatom silica frustules at nanometer-to-micrometer scales makes them of interest for a wide range of industrial applications. For some of these applications, a specific size range in nanostructure is required and may be achieved by selecting species with the desired properties. However, as ...

journal_title：Applied microbiology and biotechnology

authors： Su Y,Lundholm N,Ellegaard M

更新日期：2018-07-01 00:00:00

abstract：:This study evaluated the effect of inorganic mercury (Hg) on bacterial community and diversity in different soils. Three soils-neutral, alkaline and acidic-were spiked with six different concentrations of Hg ranging from 0 to 200 mg kg-1 and aged for 90 days. At the end of the ageing period, 18 samples from three diff...

journal_title：Applied microbiology and biotechnology

authors： Mahbub KR,Subashchandrabose SR,Krishnan K,Naidu R,Megharaj M

更新日期：2017-03-01 00:00:00

abstract：:Gluconic acid secretion mediated by the direct oxidation of glucose by pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) is responsible for mineral phosphate solubilization in Gram-negative bacteria. Herbaspirillum seropedicae Z67 (ATCC 35892) genome encodes GDH apoprotein but lacks genes for the bi...

journal_title：Applied microbiology and biotechnology

authors： Wagh J,Shah S,Bhandari P,Archana G,Kumar GN

更新日期：2014-06-01 00:00:00

abstract：:Sulfadiazine (SDZ) is an antibiotic frequently administered to livestock, and it alters microbial communities when entering soils with animal manure, but understanding the interactions of these effects to the prevailing climatic regime has eluded researchers. A climatic factor that strongly controls microbial activity...

journal_title：Applied microbiology and biotechnology

authors： Reichel R,Radl V,Rosendahl I,Albert A,Amelung W,Schloter M,Thiele-Bruhn S

更新日期：2014-01-01 00:00:00

abstract：:Synthetic logs were vacuum-soaked or regular-soaked to determine the effects of soaking on yield and mushroom size, log weight variability and water distribution within the log. Yields (g/log) from substrates vacuum-soaked were higher by 26.7%, 18.6% and 35.8% (mean = 27.2%) for crops I, II and III, respectively, when...

journal_title：Applied microbiology and biotechnology

authors： Royse DJ,Rhodes TW,Sanchez JE

更新日期：2002-01-01 00:00:00

abstract：:To seek more information on function of two-component regulatory systems (TCSs) in Streptomyces coelicolor, a dozen TCS-knockout mutants were generated, and phenotype changes were determined. One TCS (SCO5403/5404)-deleted mutant with phenotype change was obtained. Here, we report the characterization of this novel TC...

journal_title：Applied microbiology and biotechnology

authors： Lu Y,Wang W,Shu D,Zhang W,Chen L,Qin Z,Yang S,Jiang W

更新日期：2007-12-01 00:00:00

abstract：:Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four d...

journal_title：Applied microbiology and biotechnology

authors： Bradfield MF,Nicol W

更新日期：2016-11-01 00:00:00

abstract：:Seed-based expression system is an attractive platform for the production of recombinant proteins in molecular farming. Despite the many advantages of molecular farming, little is known about the effect of the different subcellular accumulation of recombinant proteins on the endoplasmic reticulum (ER) quality control ...

journal_title：Applied microbiology and biotechnology

authors： Wang D,Ma J,Sun D,Li H,Jiang C,Li X

更新日期：2015-08-01 00:00:00

abstract：:Two bioactive O-methylflavonoids, sakuranetin (7-O-methylnaringenin) and ponciretin (7-O-methylnaringenin), were synthesized in Escherichia coli. Sakuranetin inhibits germination of Magnaporthe grisea, and ponciretin is a potential inhibitor of Helicobacter pylori. To achieve this, we reconstructed the naringenin bios...

journal_title：Applied microbiology and biotechnology

authors： Kim MJ,Kim BG,Ahn JH

更新日期：2013-08-01 00:00:00

abstract：:Phosphate (P)-solubilizing microorganisms as a group form an important part of the microorganisms, which benefit plant growth and development. Growth promotion and increased uptake of phosphate are not the only mechanisms by which these microorganisms exert a positive effect on plants. Microbially mediated solubilizat...

journal_title：Applied microbiology and biotechnology

authors： Vassilev N,Vassileva M,Nikolaeva I

更新日期：2006-06-01 00:00:00

abstract：:Enantioselective oxidation of racemic phenyl-1,2-ethanediol into (R)-(-)-mandelic acid by a newly isolated Brevibacterium lutescens CCZU12-1 was demonstrated. It was found that optically active (R)-(-)-mandelic acid (e.e.p > 99.9 %) is produced leaving the other enantiomer (S)-(+)-phenyl-1,2-ethanediol intact. Using f...

journal_title：Applied microbiology and biotechnology

authors： He YC,Ma CL,Zhang X,Li L,Xu JH,Wu MX

更新日期：2013-08-01 00:00:00

abstract：:The photocatalytic properties of titanium dioxide are well known and have many applications including the removal of organic contaminants and production of self-cleaning glass. There is an increasing interest in the application of the photocatalytic properties of TiO(2) for disinfection of surfaces, air and water. Rev...

journal_title：Applied microbiology and biotechnology

authors： Foster HA,Ditta IB,Varghese S,Steele A

更新日期：2011-06-01 00:00:00

abstract：:A recombinant form of the sweet-tasting protein thaumatin has been produced in the filamentous fungus Aspergillus niger var. awamori. Expression cassettes containing a synthetic gene encoding thaumatin II were prepared and used to transform Aspergillus niger var. awamori strain NRRL312. Several fungal strains capable ...

journal_title：Applied microbiology and biotechnology

authors： Faus I,del Moral C,Adroer N,del Río JL,Patiño C,Sisniega H,Casas C,Bladé J,Rubio V

更新日期：1998-04-01 00:00:00

abstract：:Secretion of fusion proteins composed of cytoplasmic protein dihydrofolate reductase (DHFR) and the Escherichia coli alpha-haemolysin (HlyA) C-terminal sequence was examined through the haemolysin secretion machinery of E. coli. DHFR of various lengths was combined with the HlyA C-terminal region, and both secretion a...

journal_title：Applied microbiology and biotechnology

authors： Nakano H,Kawakami Y,Nishimura H

更新日期：1992-09-01 00:00:00

abstract：:Bionanocapsules (BNCs) are hollow nanoscale particles composed of L protein of the hepatitis B virus surface antigen that represent specific affinity for human hepatocytes. BNCs can transfer genes and drugs into human hepatocytes efficiently and specifically. BNC can be expressed in yeast cells. In this study, we deve...

journal_title：Applied microbiology and biotechnology

authors： Shishido T,Muraoka M,Ueda M,Seno M,Tanizawa K,Kuroda S,Fukuda H,Kondo A

更新日期：2006-12-01 00:00:00

abstract：:The alkali-soluble glucan of the yeast cell wall contains beta-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of beta-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutant...

journal_title：Applied microbiology and biotechnology

authors： Ha CH,Lim KH,Kim YT,Lim ST,Kim CW,Chang HI

更新日期：2002-03-01 00:00:00

abstract：:The long-term usage of antibiotics has resulted in the evolution of multidrug-resistant bacteria. Unlike antibiotics, anti-virulence approaches target bacterial virulence without affecting cell viability, which may be less prone to develop drug resistance. Staphylococcus aureus is a major human pathogen that produces ...

journal_title：Applied microbiology and biotechnology

authors： Lee K,Lee JH,Kim SI,Cho MH,Lee J

更新日期：2014-11-01 00:00:00

abstract：:Catharanthus roseus (L.) G. Don is a plant species known for its production of a variety of terpenoid indole alkaloids, many of which have pharmacological activities. Production of catharanthine in cell cultures or in hairy roots established by transformation with Agrobacterium rhizogenes is of interest because cathar...

journal_title：Applied microbiology and biotechnology

authors： Zhou ML,Hou HL,Zhu XM,Shao JR,Wu YM,Tang YX

更新日期：2011-08-01 00:00:00

abstract：:Glycosyltransferases (GTs)-mediated glycodiversification studies have drawn significant attention recently, with the goal of generating bioactive compounds with improved pharmacological properties by diversifying the appended sugars. The key to achieving glycodiversification is to identify natural and/or engineered fl...

journal_title：Applied microbiology and biotechnology

authors： Li L,Pan G,Zhu X,Fan K,Gao W,Ai G,Ren J,Shi M,Olano C,Salas JA,Yang K

更新日期：2017-07-01 00:00:00