Abstract：
:Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four different dilution rates. Glucose-6-phosphate dehydrogenase assays were performed on cell extracts derived from in situ removal of biofilm at each steady state. The results of the assays were coupled to a kinetic model that revealed an increase in oxidative pentose phosphate pathway (OPPP) flux relative to total substrate flux with increasing SA titre, for both substrates. Furthermore, applying metabolite concentration data to metabolic flux models that include the OPPP revealed similar flux relationships to those observed in the experimental kinetic analysis. A relative increase in OPPP flux produces additional reduction power that enables increased flux through the reductive branch of the TCA cycle, leading to increased SA yields, reduced by-product formation and complete closure of the overall redox balance.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Bradfield MF,Nicol Wdoi
10.1007/s00253-016-7763-6subject
Has Abstractpub_date
2016-11-01 00:00:00pages
9641-9652issue
22eissn
0175-7598issn
1432-0614pii
10.1007/s00253-016-7763-6journal_volume
100pub_type
杂志文章abstract：:Although a high number of chickens carry Campylobacter jejuni, the mechanistic action of colonization in the intestine is still poorly understood. The current study was therefore designed to investigate the effects of C. jejuni on glucose uptake, amino acids availability in digesta, and intracellular calcium [Ca(2+)]i...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
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更新日期：2014-04-01 00:00:00
abstract：:The fungal secretome comprises various oxidative enzymes participating in the degradation of lignocellulosic biomass as a central step in carbon recycling. Among the secreted enzymes, aryl-alcohol oxidases (AAOs) are of interest for biotechnological applications including production of bio-based precursors for plastic...
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更新日期：2020-11-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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abstract：:Agar is a major cell wall carbohydrate of red macroalgae (Rhodophyta). Sugars derived from agar, such as agarooligosaccharides (AOSs), neoagarooligosaccharides (NAOSs), neoagarobiose (NAB), and 3,6-anhydro-L-galactose (L-AHG), possess various physiological activities. These agar-derived sugars can be produced by hydro...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2017-07-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：2017-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00253-007-0949-1
更新日期：2007-07-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-007-1099-1
更新日期：2007-10-01 00:00:00
abstract：:A set of filamentous fungi (42 strains) was screened for alpha-N-acetylgalactosaminidase activity, and a series of inducers and different cultivation conditions were tested. Enzyme production by the best producer Aspergillus niger CCIM K2 was optimized and scaled up. alpha-N-Acetylgalactosaminidase was purified to app...
journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-008-1485-3
更新日期：2008-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00253-014-6292-4
更新日期：2015-04-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-015-7223-8
更新日期：2016-05-01 00:00:00
abstract：:Understanding the effect of conserved domains reconstruction of alginate lyases on action mode is essential for their application and in-depth study. We report the expression and action mode of recombinant alginate lyase (AlyM) and its conserved domain reconstruction forms (AlyMΔCBM, cAlyM, and AlyMΔ58C). The enzymati...
journal_title：Applied microbiology and biotechnology
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更新日期：2019-01-01 00:00:00
abstract：:Biorefineries allow the production of value-added chemicals, yet this also causes the formation of considerable amounts of wastewater that require suitable treatment. These biorefinery wastewaters often contain a high salinity, which inhibits methanogenesis. In this research, molasses were used to mimic these waste st...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7503-y
更新日期：2016-06-01 00:00:00
abstract：:Rapid sugar consumption is important for the microbial production of chemicals and fuels. Here, we show that overexpression of the NADH dehydrogenase gene (ndh) increased glucose consumption rate in Corynebacterium glutamicum under oxygen-deprived conditions through investigating the relationship between the glucose c...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-015-6540-2
更新日期：2015-07-01 00:00:00
abstract：:In recent years, a number of studies have been done on the physiological effects of water-soluble polysaccharides (WSPS) and their antitumor and immuno-enhancing properties. Many edible mushrooms, in particular those rich in WSPS, not only taste good but also contain ingredients beneficial to the physiology of the hum...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-004-1833-x
更新日期：2005-06-01 00:00:00
abstract：:The capability of lactic acid bacteria (LAB) to produce exopoly- and oligosaccharides was and is the subject of expanding research efforts. Due to their physicochemical properties and health-promoting potential, exopoly- and oligosaccharides from food-grade LAB can be used in the food and other industries and may have...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2006-08-01 00:00:00
abstract：:A strong promoter increases transcription of the genes of interest and enhances the production of various valuable substances. For a hyperthermophilic archaeon Thermococcus onnurineus NA1, which can produce H2 from carbon monoxide oxidation, we searched for a novel endogenous strong promoter by transcriptome analysis ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-015-6444-1
更新日期：2015-05-01 00:00:00
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更新日期：2011-03-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/s002530050964
更新日期：1997-05-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/BF00164738
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abstract：:Aspergillus oryzae is a fungus used extensively in the fermentation industry. We constructed cDNA microarrays comprising 2,070 highly expressed cDNAs selected from the approximately 6,000 non-redundant expressed sequence tags (ESTs) in the A. oryzae EST database (http://www.aist.go.jp/RIODB/ffdb/index.html). Using the...
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更新日期：2004-07-01 00:00:00
abstract：:In a previous study, a marine isolate Clostridium sp. EDB2 degraded 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20) under anaerobic conditions (Bhushan B, Halasz A, Thiboutot S, Ampleman G, Hawari J (2004c) Chemotaxis-mediated biodegradation of cyclic nitramine explosives RDX, HMX, and CL-20 by Clos...
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pub_type： 杂志文章
doi：10.1007/s00253-005-1992-4
更新日期：2005-12-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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pub_type： 杂志文章
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更新日期：2007-09-01 00:00:00
abstract：:Hemidesmus indicus (L.) R. Br. ex Schult., commonly known as Indian sarsaparilla or Anantamul, is an ethnopharmacologically important medicinal plant from the family Apocynaceae. Biosynthesis of an array of bioactive compounds such as methoxybenzaldehydes and their derivatives has been accountable for its intensive me...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2020-10-01 00:00:00
abstract：:Live attenuated bacteria delivered orally are interesting tools for mucosal immunization. The objective of this study was to construct a novel counter-selection platform based on an attenuated wild-type Escherichia coli (E. coli) strain and to utilize it for the delivery of LTR192G-STaA13Q fusion protein as an oral va...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-014-6099-3
更新日期：2015-01-01 00:00:00
