Release of thioredoxin from Saccharomyces cerevisiae with environmental stimuli: solubilization of thioredoxin with ethanol.

abstract：:Soil metagenome constitutes a reservoir for discovering novel enzymes from the unculturable microbial diversity. From three plant rhizosphere metagenomic libraries comprising a total of 142,900 members of recombinant plasmids, we obtained 14 recombinant fosmids that exhibited lipolytic activity. A selected recombinant...

journal_title：Applied microbiology and biotechnology

authors： Lee MH,Hong KS,Malhotra S,Park JH,Hwang EC,Choi HK,Kim YS,Tao W,Lee SW

更新日期：2010-11-01 00:00:00

abstract：:Lactobacillus species are attractive hosts for the expression of heterologous proteins, antigens, vaccines, and drugs due to their GRAS (generally recognized as safe) status. The bioengineering techniques open new possibilities of improving Lactobacillus strains. In this regard, the control of the gene expression in L...

journal_title：Applied microbiology and biotechnology

authors： Peirotén Á,Landete JM

更新日期：2020-05-01 00:00:00

abstract：:The development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable and reproducible printing of cells. This review outlines the general principles and current progress a...

journal_title：Applied microbiology and biotechnology

authors： Ferris CJ,Gilmore KG,Wallace GG,In het Panhuis M

更新日期：2013-05-01 00:00:00

abstract：:Myxobacteria are Gram-negative soil microorganisms that prey on other microorganisms. Myxobacteria have significant potential for applications in biotechnology because of their extraordinary ability to produce natural products such as secondary metabolites. Myxobacteria also stand out as model organisms for the study ...

journal_title：Applied microbiology and biotechnology

authors： Ueki T,Inouye S

更新日期：2006-08-01 00:00:00

abstract：:Conventional acetone-butanol-ethanol (ABE) fermentation coupled with gas stripping is conducted under strict anaerobic conditions. In this work, a fed-batch ABE fermentation integrated with gas stripping (FAFIGS) system using a non-strict anaerobic butanol-producing symbiotic system, TSH06, was investigated for the ef...

journal_title：Applied microbiology and biotechnology

authors： Lin Z,Liu H,Yan X,Zhou Y,Cheng K,Zhang J

更新日期：2017-11-01 00:00:00

abstract：:We report here the creation of a modular, plasmid-based protein expression system utilizing elements of the native Rhodobacter puf promoter in a BioBrick(TM)-based vector system with DsRed encoding a red fluorescent reporter protein. A suite of truncations of the puf promoter were made to assess the influence of diffe...

journal_title：Applied microbiology and biotechnology

authors： Tikh IB,Held M,Schmidt-Dannert C

更新日期：2014-04-01 00:00:00

abstract：:Mating tests, internal transcribed spacer (ITS) sequence analysis, intergenic spacer 1-restriction fragment length polymorphism (IGS1-RFLP), IGS1 sequence analysis, and IGS2-RFLP analysis were carried out on isolates of 17 morphologically different Pleurotus mushrooms collected on Ferula sinkiangensis. The isolates we...

journal_title：Applied microbiology and biotechnology

authors： Zhang JX,Huang CY,Ng TB,Wang HX

更新日期：2006-07-01 00:00:00

abstract：:The efficient production of recombinant proteins such as antibodies typically involves the screening of an extravagant number of clones in order to finally select a stable and high-producing cell line. Thereby, the underlying principles of a powerful protein machinery, but also potential expression limitations, often ...

journal_title：Applied microbiology and biotechnology

authors： Reinhart D,Sommeregger W,Debreczeny M,Gludovacz E,Kunert R

更新日期：2014-07-01 00:00:00

abstract：:Neuritin (also known as candidate plasticity gene 15 (cpg15)) is a neurotrophic factor that was recently discovered in a screen aimed at identifying genes involved in activity-dependent synaptic plasticity. Neuritin plays multiple roles in both neural development (Chen et al. Zhonghua Yan Ke Za Zhi 46:978-983 2010; Co...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Zhang S,Xian L,Tang J,Zhu J,Cui L,Li S,Yang L,Huang J

更新日期：2015-10-01 00:00:00

abstract：:Electron transfer pathways for azoreduction by S. decolorationis S12 were studied using a mutant S12-22 which had a transposon insertion in ccmA. The results imply that there are two different pathways for electron transport to azo bonds. The colony of S12-22 was whitish and incapable of producing mature c-type cytoch...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Xu M,Wei J,Sun G

更新日期：2010-03-01 00:00:00

abstract：:The fungus Colletotrichum lindemuthianum is the causal agent of anthracnose in the common bean (Phaseolus vulgaris), and anthracnose is one of the most devastating diseases of this plant species. However, little is known about the proteins that are essential for the fungus-plant interactions. Knowledge of the fungus' ...

journal_title：Applied microbiology and biotechnology

authors： de Queiroz CB,Correia HLN,Santana MF,Batista DS,Vidigal PMP,Brommonschenkel SH,de Queiroz MV

更新日期：2019-03-01 00:00:00

abstract：:Conductive materials can facilitate syntrophic methane (CH4) production by improving direct interspecies electron transfer. The effect of a conductive material, ferroferric oxide (Fe3O4), on the anaerobic treatment of tryptone-based high-strength wastewater was investigated in two anaerobic sequencing batch reactors (...

journal_title：Applied microbiology and biotechnology

authors： Yin Q,He K,Liu A,Wu G

更新日期：2017-05-01 00:00:00

abstract：:Biofilms are widely used in wastewater treatment for their particular enhancement of nitrogen removal and other significant advantages. In this study, the diversity and potential functions of nitrogen removal bacteria in suspended activated sludge (AS) and biofilm of a full-scale hybrid reactor were uncovered by metag...

journal_title：Applied microbiology and biotechnology

authors： Chao Y,Mao Y,Yu K,Zhang T

更新日期：2016-09-01 00:00:00

abstract：:Lipase has been used widely in industry. In this study, we have constructed two recombinant Saccharomyces cerevisiae strains displaying two active lipases on the cell surface by cell surface engineering. The genes encoding Yarrowia lipolytica lipases Lip7 and Lip8 were fused with the gene encoding small binding subuni...

journal_title：Applied microbiology and biotechnology

authors： Liu WS,Pan XX,Jia B,Zhao HY,Xu L,Liu Y,Yan YJ

更新日期：2010-10-01 00:00:00

abstract：:Forty-eight single-use stirred tank bioreactors on a 10-mL scale operated in a magnetically inductive driven bioreaction block and automated with a liquid handler were applied for discrimination of different riboflavin producing Bacillus subtilis strains based on their performances in the parallel fed-batch processes....

journal_title：Applied microbiology and biotechnology

authors： Vester A,Hans M,Hohmann HP,Weuster-Botz D

更新日期：2009-08-01 00:00:00

abstract：:The knowledge of biosynthesis gene clusters, production improving methods, and bioactivity mechanisms is very important for the development of filamentous fungi metabolites. Metabolic engineering and heterologous expression methods can be applied to improve desired metabolite production, when their biosynthesis pathwa...

journal_title：Applied microbiology and biotechnology

authors： Yin Y,Cai M,Zhou X,Li Z,Zhang Y

更新日期：2016-09-01 00:00:00

abstract：:In light of emerging antibiotic resistance, bacterial cell wall lytic enzymes are promising antimicrobial agents that degrade bacterial peptidoglycan while specifically recognizing the target bacterium. The efficacy of lytic enzymes against several multi-drug-resistant pathogens infecting humans has led to many effort...

journal_title：Applied microbiology and biotechnology

authors： Bhagwat A,Mixon M,Collins CH,Dordick JS

更新日期：2020-11-01 00:00:00

abstract：:Decolorization of the representative anthraquinone dye, Remazol Brilliant Blue R (RBBR) was assessed to determine the practical potential of crude recombinant dye-decolorizing peroxidase generated by Aspergillus oryzae (rDyP) in term of turnover capacity of rDyP. The turnover capacity, defined as the milligram of RBBR...

journal_title：Applied microbiology and biotechnology

authors： Shakeri M,Shoda M

更新日期：2007-09-01 00:00:00

abstract：:Escherichia coli K-12 was cultured under anaerobic conditions to form biofilm on carbon fiber electrodes in glucose-containing medium. The anodic current increased with the development of the biofilm and depended on the glucose concentration. Cyclic voltammetric results support the presence of a redox compound(s) excr...

journal_title：Applied microbiology and biotechnology

authors： Wang YF,Tsujimura S,Cheng SS,Kano K

更新日期：2007-10-01 00:00:00

abstract：:Aflatoxins are toxic and carcinogenic metabolites of several Aspergillus species. The effect of nitrate on aflatoxin production and expression of the key regulatory genes involved in aflatoxin biosynthesis, aflR and aflJ, were compared among isolates of the S(B) and S(BG) strains of A. flavus. Aflatoxin production by ...

journal_title：Applied microbiology and biotechnology

authors： Ehrlich KC,Cotty PJ

更新日期：2002-10-01 00:00:00

abstract：:One of the major aims of bioprocess engineering is the real-time monitoring of important process variables. This is the basis of precise process control and is essential for high productivity as well as the exact documentation of the overall production process. Infrared spectroscopy is a powerful analytical technique ...

journal_title：Applied microbiology and biotechnology

authors： Landgrebe D,Haake C,Höpfner T,Beutel S,Hitzmann B,Scheper T,Rhiel M,Reardon KF

更新日期：2010-09-01 00:00:00

abstract：:Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system. However, it is unknown whether sialylated lactuloses have modulatory effects on the intestinal microbiota. ...

journal_title：Applied microbiology and biotechnology

authors： Song M,Zeng J,Jia T,Gao H,Zhang R,Jiang J,Li G,Su T

更新日期：2019-11-01 00:00:00

abstract：:A two-stage two-stream chemostat system and a two-stage two-stream immobilized upflow packed-bed reactor system were used for the study of lactic acid production by Lactobacillus casei subsp casei. A mixing ratio of D12/D2 = 0.5 (D = dilution rate) resulted in optimum production, making it possible to generate continu...

journal_title：Applied microbiology and biotechnology

authors： Bruno-Bárcena JM,Ragout AL,Córdoba PR,Siñeriz F

更新日期：1999-03-01 00:00:00

abstract：:Agar is a major polysaccharide of red algal cells and is mainly decomposed into neoagarobiose by the co-operative effort of β-agarases. Neoagarobiose is hydrolyzed into monomers, D-galactose and 3,6-anhydro-L-galactose, via a microbial oxidative process. Therefore, the enzyme, 1,3-α-3,6-anhydro-L-galactosidase (α-neoa...

journal_title：Applied microbiology and biotechnology

authors： Asghar S,Lee CR,Park JS,Chi WJ,Kang DK,Hong SK

更新日期：2018-10-01 00:00:00

abstract：:After crude protein of the marine yeast strains maintained in this laboratory was estimated by the method of Kjehldahl, we found that the G7a strain which was identified to be a strain of Cryptococcus aureus according to the routine identification and molecular methods contained high level of protein and could grow on...

journal_title：Applied microbiology and biotechnology

authors： Gao L,Chi Z,Sheng J,Ni X,Wang L

更新日期：2007-12-01 00:00:00

abstract：:Clavulanic acid is a secondary metabolite produced by Streptomyces clavuligerus. It possesses a clavam structure and a characteristic 3R,5R stereochemistry essential for action as a beta-lactamase inhibitory molecule. It is produced from glyceraldehyde-3-phosphate and arginine in an eight step biosynthetic pathway. Th...

journal_title：Applied microbiology and biotechnology

authors： Liras P,Rodríguez-García A

更新日期：2000-10-01 00:00:00

abstract：:Fungi of the genus Trichoderma are soilborne, green-spored ascomycetes that can be found all over the world. They have been studied with respect to various characteristics and applications and are known as successful colonizers of their habitats, efficiently fighting their competitors. Once established, they launch th...

journal_title：Applied microbiology and biotechnology

authors： Schuster A,Schmoll M

更新日期：2010-07-01 00:00:00

abstract：:Cultivation of Aspergillus terreus ATCC 20542 in a stirred tank bioreactor was performed to induce the biosynthesis of secondary metabolites and provide the bioprocess-related insights into the metabolic capabilities of the investigated strain. The activation of biosynthetic routes was attempted by the diversification...

journal_title：Applied microbiology and biotechnology

authors： Boruta T,Bizukojc M

更新日期：2016-04-01 00:00:00

abstract：:A yeast protein fragment complementation assay (PCA) system based on dihydrofolate reductase (DHFR) is difficult to be operated because it is not as sensitive to trimethoprim (TMP) as the system using a prokaryotic microorganism. Here, the PCA system using DHFR, specific inhibitors, and a substrate in the yeast Saccha...

journal_title：Applied microbiology and biotechnology

authors： Shibasaki S,Sakata K,Ishii J,Kondo A,Ueda M

更新日期：2008-09-01 00:00:00

abstract：:Acetobacter xylinum BPR2001 produces water-insoluble bacterial cellulose (BC). Using a pH sensor for the accurate control of pH, which is one of the most critical factors for efficient BC production, is difficult especially in a baffled shake-flask and an airlift reactor. The buffering capacity of corn steep liquor (C...

journal_title：Applied microbiology and biotechnology

authors： Noro N,Sugano Y,Shoda M

更新日期：2004-04-01 00:00:00