Structural and functional characterization of the recombinant thioredoxin reductase from Candida albicans as a potential target for vaccine and drug design.

abstract：:The 3D structures of Fim H and PapG proteins complexed with the host carbohydrate receptor demonstrate that both utilize binding-pocket asparagines for contact or stabilization with the carbohydrate. Pretreatment of whole bacteria with asparaginase resulted in decreased fimbriae-mediated attachment to urinary epitheli...

journal_title：Applied microbiology and biotechnology

authors： Wehner MR,Koch SR,Kindinger L,Nelson N,Cowan MM

更新日期：2004-11-01 00:00:00

abstract：:A strong promoter increases transcription of the genes of interest and enhances the production of various valuable substances. For a hyperthermophilic archaeon Thermococcus onnurineus NA1, which can produce H2 from carbon monoxide oxidation, we searched for a novel endogenous strong promoter by transcriptome analysis ...

journal_title：Applied microbiology and biotechnology

authors： Lee SH,Kim MS,Jung HC,Lee J,Lee JH,Lee HS,Kang SG

更新日期：2015-05-01 00:00:00

abstract：:Significant decreases in the apparent viscosity of a bacterial biofilm suspension were measured following addition of sodium, potassium, magnesium, or calcium salts, whereas iron salts increased the viscosity. Electrostatic interactions contribute to biofilm cohesion and iron cations are potent crosslinkers of the bio...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Stewart PS

更新日期：2002-09-01 00:00:00

abstract：:In industry, filamentous fungi have a prominent position as producers of economically relevant primary or secondary metabolites. Particularly, the advent of genetic engineering of filamentous fungi has led to a growing number of molecular tools to adopt filamentous fungi for biotechnical applications. Here, we summari...

journal_title：Applied microbiology and biotechnology

authors： Kluge J,Terfehr D,Kück U

更新日期：2018-08-01 00:00:00

abstract：:Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

journal_title：Applied microbiology and biotechnology

authors： Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

更新日期：2006-10-01 00:00:00

abstract：:A thermophilic Bacillus coagulans WCP10-4 with tolerance to high concentration of glucose was isolated from soil and used to produce optically pure L-lactic acid from glucose and starch. In batch fermentation at pH 6.0, 240 g/L of glucose was completely consumed giving 210 g/L of L-lactic acid with a yield of 95 % and...

journal_title：Applied microbiology and biotechnology

authors： Zhou X,Ye L,Wu JC

更新日期：2013-05-01 00:00:00

abstract：:Leuconostoc carnosum 4010 is a protective culture for meat products. It kills the foodborne pathogen Listeria monocytogenes by producing two class IIa (pediocin-like) bacteriocins, leucocin A and leucocin C. The genes for leucocin A production have previously been characterised from Leuconostoc gelidum UAL 187, wherea...

journal_title：Applied microbiology and biotechnology

authors： Wan X,Li R,Saris PE,Takala TM

更新日期：2013-04-01 00:00:00

abstract：:In this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>20...

journal_title：Applied microbiology and biotechnology

authors： Mendes FS,González-Pajuelo M,Cordier H,François JM,Vasconcelos I

更新日期：2011-11-01 00:00:00

abstract：:Four types of cell-surface-engineered yeast Saccharomyces cerevisiae displaying glucoamylase, namely, systems A, B, C, and D, were constructed to evaluate their performance in direct ethanol fermentation from raw corn starch. Systems A and B were glucoamylase-displaying nonflocculent yeast (YF237) types that secrete a...

journal_title：Applied microbiology and biotechnology

authors： Khaw TS,Katakura Y,Koh J,Kondo A,Ueda M,Shioya S

更新日期：2006-05-01 00:00:00

abstract：:Several studies support the use of probiotics for the treatment of minor gastrointestinal problems in infants. Positive effects on newborn colics have been evidenced after administration of Lactobacillus strains, whereas no studies have been reported regarding the use of bifidobacteria for this purpose. This work was ...

journal_title：Applied microbiology and biotechnology

authors： Aloisio I,Santini C,Biavati B,Dinelli G,Cencič A,Chingwaru W,Mogna L,Di Gioia D

更新日期：2012-12-01 00:00:00

abstract：:The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic ni...

journal_title：Applied microbiology and biotechnology

authors： Saha BC

更新日期：2006-10-01 00:00:00

abstract：:Active penicillin acylase from Kluyvera citrophila strain ATCC 21,285 consists of two different alpha- and beta-subunits derived from a single precursor by post-translational processing. Using the chemical mutagen hydroxylamine we have treated plasmid pYKD59 containing the active penicillin acylase gene (pga) from K. ...

journal_title：Applied microbiology and biotechnology

authors： Prieto I,Rodríguez MC,Márquez G,Pérez-Aranda A,Barbero JL

更新日期：1992-02-01 00:00:00

abstract：:A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with ...

journal_title：Applied microbiology and biotechnology

authors： Wang HX,Ng TB

更新日期：2006-12-01 00:00:00

abstract：:Mannitol is a natural low-calorie sugar alcohol produced by certain (micro)organisms applicable in foods for diabetics due to its zero glycemic index. In this work, we evaluated mannitol production and yield by the fruit origin strain Fructobacillus tropaeoli CRL 2034 using response surface methodology with central co...

journal_title：Applied microbiology and biotechnology

authors： Ruiz Rodríguez LG,Aller K,Bru E,De Vuyst L,Hébert EM,Mozzi F

更新日期：2017-08-01 00:00:00

abstract：:The lysin gene (lysA2) of the Lactobacillus casei bacteriophage A2 was cloned and expressed in Escherichia coli. LysA2 is an endopeptidase that hydrolyzes the bond between the terminal D: -alanine of the peptidoglycan tetrapeptide and the aspartic acid residue that forms the bridge with the L: -lysine of a neighboring...

journal_title：Applied microbiology and biotechnology

authors： Ribelles P,Rodríguez I,Suárez JE

更新日期：2012-04-01 00:00:00

abstract：:Streptomyces viridochromogenes Tü494 produces the antibiotic phosphinothricyl-alanyl-alanine (Ptt). Ptt-non-producing mutants were isolated following N-methyl-N'-nitro-N-nitrosoguanidine (NTG) or UV light treatment of spore suspensions. In co-synthesis and bioconversion experiments the mutational blocks in the biosynt...

journal_title：Applied microbiology and biotechnology

authors： Alijah R,Dorendorf J,Talay S,Pühler A,Wohlleben W

更新日期：1991-03-01 00:00:00

abstract：:Although several bacterial lignin-oxidising enzymes have been discovered in recent years, it is not yet clear whether different lignin-degrading bacteria use similar mechanisms for lignin oxidation and degradation of lignin fragments. Genome sequences of 13 bacterial lignin-oxidising bacteria, including new genome seq...

journal_title：Applied microbiology and biotechnology

authors： Granja-Travez RS,Persinoti GF,Squina FM,Bugg TDH

更新日期：2020-04-01 00:00:00

abstract：:Microbes engineered to display heterologous proteins could be useful biotechnological tools for protein engineering, lignocellulose degradation, biocatalysis, bioremediation, and biosensing. Bacillus subtilis is a promising host to display proteins, as this model Gram-positive bacterium is genetically tractable and al...

journal_title：Applied microbiology and biotechnology

authors： Huang GL,Gosschalk JE,Kim YS,Ogorzalek Loo RR,Clubb RT

更新日期：2018-08-01 00:00:00

abstract：:D-lactic acid is used as a monomer in the production of poly-D-lactic acid (PDLA), which is used to form heat-resistant stereocomplex poly-lactic acid. To produce cost-effective D-lactic acid by using all sugars derived from biomass efficiently, xylose-assimilating genes encoding xylose isomerase and xylulokinase were...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Vadlani PV,Kumar A,Hardwidge PR,Govind R,Tanaka T,Kondo A

更新日期：2016-01-01 00:00:00

abstract：:Potato pulp is a poorly utilized, high-volume co-processing product resulting from industrial potato starch manufacturing. Potato pulp mainly consists of the tuber plant cell wall material and is particularly rich in pectin, notably galactan branched rhamnogalacturonan I type pectin which has previously been shown to ...

journal_title：Applied microbiology and biotechnology

authors： Thomassen LV,Vigsnæs LK,Licht TR,Mikkelsen JD,Meyer AS

更新日期：2011-05-01 00:00:00

abstract：:Nitrite-dependent anaerobic methane oxidation (N-DAMO), which couples anaerobic methane oxidation and nitrite reduction, is a recently discovered bioprocess coupling microbial nitrogen and carbon cycles. The discovery of this microbial process challenges the traditional knowledge of global methane sinks and nitrogen l...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Shen L,He Z,Hu J,Cai Z,Zheng P,Hu B

更新日期：2017-11-01 00:00:00

abstract：:A histidine oligopeptide (hexa-His) with the ability to chelate divalent heavy metal ions was displayed on the yeast cell surface for the purpose of enhanced adsorption of heavy metal ions. We genetically fused a hexa-His-encoding gene with the gene encoding the C-terminal half of alpha-agglutinin that includes a glyc...

journal_title：Applied microbiology and biotechnology

authors： Kuroda K,Shibasaki S,Ueda M,Tanaka A

更新日期：2001-12-01 00:00:00

abstract：:Cytochromes P450 (CYPs) belong to the superfamily of heme b containing monooxygenases with currently more than 21,000 members. These enzymes accept a vast range of organic molecules and catalyze diverse reactions. These extraordinary capabilities of CYP systems that are unmet by other enzymes make them attractive for ...

journal_title：Applied microbiology and biotechnology

authors： Bernhardt R,Urlacher VB

更新日期：2014-01-01 00:00:00

abstract：:Mutagenesis and selection were applied to a strain of Penicillium echinulatum by treating conidia with hydrogen peroxide or 1,2,7,8-diepoxyoctane and then by incubating the conidia for 48 h in broth containing microcrystalline cellulose washed in 0.5% (w/v) aqueous 2-deoxyglucose before plating them onto cellulose aga...

journal_title：Applied microbiology and biotechnology

authors： Dillon AJ,Zorgi C,Camassola M,Henriques JA

更新日期：2006-05-01 00:00:00

abstract：:The alphaproteobacterial Roseobacter clade (Rhodobacterales) is one of the most important global players in carbon and sulfur cycles of marine ecosystems. The remarkable metabolic versatility of this bacterial lineage provides access to diverse habitats and correlates with a multitude of extrachromosomal elements. Fou...

journal_title：Applied microbiology and biotechnology

authors： Petersen J,Frank O,Göker M,Pradella S

更新日期：2013-04-01 00:00:00

abstract：:The natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the incorporation of non-standard amino acids (NSAAs). Proteins containing NSAAs provide enhanced or novel properties and open diverse applications. With increased attention to the recent adv...

journal_title：Applied microbiology and biotechnology

authors： Jin X,Park OJ,Hong SH

更新日期：2019-04-01 00:00:00

abstract：:This study demonstrated for the first time the possibility to remove and partially recover the Ruthenium contained in industrial effluents by using purple non sulfur bacteria (PNSB) as microbial biosorbents. Up to date, the biosorption was only claimed as possible tool for the removal of the platinum-group metals (PGM...

journal_title：Applied microbiology and biotechnology

authors： Colica G,Caparrotta S,De Philippis R

更新日期：2012-07-01 00:00:00

abstract：:Forty-eight single-use stirred tank bioreactors on a 10-mL scale operated in a magnetically inductive driven bioreaction block and automated with a liquid handler were applied for discrimination of different riboflavin producing Bacillus subtilis strains based on their performances in the parallel fed-batch processes....

journal_title：Applied microbiology and biotechnology

authors： Vester A,Hans M,Hohmann HP,Weuster-Botz D

更新日期：2009-08-01 00:00:00

abstract：:Drug-resistant bacteria are becoming an increasingly widespread problem in the clinical setting. The current pipeline of antibiotics cannot provide satisfactory options for clinicians, which brought increasing attention to the development and application of non-traditional antimicrobial substances as alternatives. Met...

journal_title：Applied microbiology and biotechnology

authors： Ye Q,Chen W,Huang H,Tang Y,Wang W,Meng F,Wang H,Zheng Y

更新日期：2020-06-01 00:00:00

abstract：:Pseudomonas aeruginosa and Staphylococcus aureus are versatile bacterial pathogens and common etiological agents in polymicrobial infections. Microbial communities containing both of these pathogens are shaped by interactions ranging from parasitic to mutualistic, with the net impact of these interactions in many case...

journal_title：Applied microbiology and biotechnology

authors： Nguyen AT,Oglesby-Sherrouse AG

更新日期：2016-07-01 00:00:00