Secretion of genetically-engineered dihydrofolate reductase from Escherichia coli using an E. coli alpha-hemolysin membrane translocation system.

abstract：:The upstream region of the cellobiohydrolase gene cbhA of Clostridium thermocellum F7 was sequenced. It was found that this region contains the previously sequenced gene celK encoding an enzyme closely related to CbhA (cellulosomal subunit S3). The presence of a putative transcription terminator in the 524-bp intergen...

journal_title：Applied microbiology and biotechnology

authors： Zverlov VV,Velikodvorskaya GA,Schwarz WH,Kellermann J,Staudenbauer WL

更新日期：1999-06-01 00:00:00

abstract：:The binaphthyl derivative cladosporol 3 was supplied from 60 to 200 mg l(-1) to shaken cultures of Cladosporium cf. cladosporioides. Compared to blank, fungal biomass was not affected by adding cladosporol till 100 mg l(-1): it rather increased at higher ratios between 150 and 200 mg l(-1). The production of the major...

journal_title：Applied microbiology and biotechnology

authors： Assante G,Bava A,Nasini G

更新日期：2006-02-01 00:00:00

abstract：:Batch and continuous cultivation of Anaerobiospirillum succiniciproducens were systematically studied for the production of succinic acid from whey. Addition of 2.5 g l(-1) yeast extract and 2.5 g l(-1) polypeptone per 10 g l(-1) whey was most effective for succinic acid production from both treated and nontreated whe...

journal_title：Applied microbiology and biotechnology

authors： Lee PC,Lee WG,Kwon S,Lee SY,Chang HN

更新日期：2000-07-01 00:00:00

abstract：:The gene ce1A, encoding an endoglucanase from the strain Bacillus sp. BP-23, was cloned and expressed in Escherichia coli. The nucleotide sequence of a 1867-bp DNA fragment containing the ce1A gene was determined, revealing an open reading frame of 1200 nucleotides that encodes a protein of 44,803 Da. The deduced amin...

journal_title：Applied microbiology and biotechnology

authors： Blanco A,Díaz P,Martínez J,Vidal T,Torres AL,Pastor FI

更新日期：1998-07-01 00:00:00

abstract：:Seasonal shifts of dissolved inorganic nitrogen (DIN) and the dynamics of microbial communities for nitrogen transformation were investigated in the water column of Chesapeake Bay. The relative abundance of nitrogen over phosphorus (N) showed a strong seasonal and spatial pattern: gradually decreased from upstream to ...

journal_title：Applied microbiology and biotechnology

authors： Hong Y,Xu X,Kan J,Chen F

更新日期：2014-04-01 00:00:00

abstract：:Biocides play an important role in the preservation of white mineral dispersions (WMD). Due to the occurrence of biocide-resistant bacteria and technical limitations in the use of biocides, new preservation strategies are required-like the enhancement of biocides by non-biocidal compounds. The aim of this study was to...

journal_title：Applied microbiology and biotechnology

authors： Di Maiuta N,Schwarzentruber P,Dow CS

更新日期：2011-01-01 00:00:00

abstract：:Stable transformants of Chromobacterium violaceum were obtained by high-voltage electroporation with a 7-kilobase binary plasmid. The technique was reliable, reproducible, and simple, with efficiencies of 10(5) transformants/microg of plasmid DNA. The electrical conditions that resulted in the highest efficiencies wer...

journal_title：Applied microbiology and biotechnology

authors： Broetto L,Cecagno R,Sant'anna FH,Weber S,Schrank IS

更新日期：2006-07-01 00:00:00

abstract：:The demand of renewable energy sources, i.e. biomass, is steadily increasing worldwide to reduce the need of fossil energy sources. Biomass such as energy crops, woody species, forestry and agricultural residues are the most common renewable energy sources. Due to uneven demand for wood fuel, the material is mostly st...

journal_title：Applied microbiology and biotechnology

authors： Noll M,Jirjis R

更新日期：2012-08-01 00:00:00

abstract：:Mycorrhizal fungi belonging to the genus Tuber produce, after the establishment of a productive interaction with a plant host, hypogeous fruitbodies of great economic value known as ''truffles''. This review summarizes the state of art on life cycle, genetic, and biotechnological investigations of Tuber spp. The ascoc...

journal_title：Applied microbiology and biotechnology

authors： Poma A,Limongi T,Pacioni G

更新日期：2006-09-01 00:00:00

abstract：:Biochemical processes in biogas plants are still not fully understood. Especially, the identification of possible bottlenecks in the complex fermentation processes during biogas production might provide potential to increase the performance of biogas plants. To shed light on the question which group of organism consti...

journal_title：Applied microbiology and biotechnology

authors： Refai S,Wassmann K,Deppenmeier U

更新日期：2014-08-01 00:00:00

abstract：:This review addresses metal uptake specifically by yeast. Metal uptake may be passive, active or both, depending on the viability of the biomass, and is influenced by a number of environmental and experimental factors. Uptake is typically accompanied by a degree of ion exchange and, under certain conditions, may be en...

journal_title：Applied microbiology and biotechnology

authors： Blackwell KJ,Singleton I,Tobin JM

更新日期：1995-08-01 00:00:00

abstract：:Calcineurin is highly conserved and regulates growth, conidiation, stress response, and pathogenicity in fungi. However, the functions of calcineurin and its regulatory network in entomopathogenic fungi are not clear. In this study, calcineurin was functionally analyzed by deleting the catalytic subunit MaCnA from the...

journal_title：Applied microbiology and biotechnology

authors： Cao Y,Du M,Luo S,Xia Y

更新日期：2014-10-01 00:00:00

abstract：:Acetic acid bacteria are well-known for their membrane-bound dehydrogenases rapidly oxidizing a variety of substrates in the periplasm. Since many acetic acid bacteria have not been successfully cultured in the laboratory yet, studying membrane-bound dehydrogenases directly from a metagenome of vinegar microbiota seem...

journal_title：Applied microbiology and biotechnology

authors： Peters B,Mientus M,Kostner D,Daniel R,Liebl W,Ehrenreich A

更新日期：2017-11-01 00:00:00

abstract：:Significant decreases in the apparent viscosity of a bacterial biofilm suspension were measured following addition of sodium, potassium, magnesium, or calcium salts, whereas iron salts increased the viscosity. Electrostatic interactions contribute to biofilm cohesion and iron cations are potent crosslinkers of the bio...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Stewart PS

更新日期：2002-09-01 00:00:00

abstract：:Bacillus subtilis 916 was an effective biocontrol agent in control rice sheath blight caused by Rhizoctonia solani. To further improve its antagonistic ability, low-energy ion implantation was applied in Bs-916. We studied the effects of different doses of N(+) implantation. The optimum dose of ion implantation for th...

journal_title：Applied microbiology and biotechnology

authors： Li D,Nie F,Wei L,Wei B,Chen Z

更新日期：2007-07-01 00:00:00

abstract：:Artificial cold adaptation of a mesophilic protease, subtilisin BPN' was attempted by means of random mutagenesis of its entire gene coupled with screening of cleared-zone-forming colonies on skim-milk plates at a low temperature. Out of sixty clones screened at 10 degrees C, one mutant enzyme (termed M-15) was found ...

journal_title：Applied microbiology and biotechnology

authors： Kano H,Taguchi S,Momose H

更新日期：1997-01-01 00:00:00

abstract：:Trehalose (α-D-glucopyranosyl-(1 → 1)-α-D-glucopyranoside) is a non-reducing disaccharide composed of two glucose molecules linked by an α,α-1,1-glycosidic bond. It possesses physicochemical properties, which account for its biological roles in a variety of prokaryotic and eukaryotic organisms and invertebrates. Inten...

journal_title：Applied microbiology and biotechnology

authors： Cai X,Seitl I,Mu W,Zhang T,Stressler T,Fischer L,Jiang B

更新日期：2018-04-01 00:00:00

abstract：:This study aimed to evaluate the effects of partial reducing rumen-protected Lys (RPLys) on rumen fermentation and microbial composition in heifers. Three ruminal fistulated Holstein Friesian bulls were used to determine the effective degradability of RPLys using an in situ method at incubation times of 0, 2, 6, 12, 1...

journal_title：Applied microbiology and biotechnology

authors： Kong F,Gao Y,Tang M,Fu T,Diao Q,Bi Y,Tu Y

更新日期：2020-08-01 00:00:00

abstract：:Demand for novel antifungal drugs for medical and agricultural uses has been increasing because of the diversity of pathogenic fungi and the emergence of drug-resistant strains. Genomic resources for various living species, including pathogenic fungi, can be utilized to develop novel and effective antifungal compounds...

journal_title：Applied microbiology and biotechnology

authors： Marui J,Yoshimi A,Hagiwara D,Fujii-Watanabe Y,Oda K,Koike H,Tamano K,Ishii T,Sano M,Machida M,Abe K

更新日期：2010-08-01 00:00:00

abstract：:CYP102A1 is an efficient medium- to long-chain fatty acid hydroxylase that is able to accept a wide range of non-natural substrates which bear no resemblance to the natural ones. 4-Hexylbenzoic acid (HBA) and 4-nonyloxybenzoic acid (NOBA) were identified as CYP102A1 substrates via screening studies using the BD Oxygen...

journal_title：Applied microbiology and biotechnology

authors： Gudiminchi RK,Smit MS

更新日期：2011-04-01 00:00:00

abstract：:Chemically synthesized 2-hydroxyethyl jasmonate (HEJA) was for the first time employed to induce the ginsenoside biosynthesis and to manipulate the product heterogeneity in plant cell cultures. The dose response and timing of HEJA elicitation were investigated in cell suspension cultures of Panax notoginseng. The opti...

journal_title：Applied microbiology and biotechnology

authors： Wang W,Zhao ZJ,Xu Y,Qian X,Zhong JJ

更新日期：2006-04-01 00:00:00

abstract：:"Milking processing" describes the cultivation of microalgae in a water-organic solvent two-phase system that consists of simultaneous fermentation and secretion of intracellular product. It is usually limited by the conflict between the biocompatibility of the organic solvent to the microorganisms and the ability of ...

journal_title：Applied microbiology and biotechnology

authors： Hu Z,Zhang X,Wu Z,Qi H,Wang Z

更新日期：2012-04-01 00:00:00

abstract：:Oenococcus oeni is the lactic acid bacteria species most commonly encountered in wine, where it develops after the alcoholic fermentation and achieves the malolactic fermentation that is needed to improve the quality of most wines. O. oeni is abundant in the oenological environment as well as in apple cider and kombuc...

journal_title：Applied microbiology and biotechnology

authors： Lorentzen MPG,Lucas PM

更新日期：2019-04-01 00:00:00

abstract：:Cupriavidus basilensis is a species with diverse metabolic capabilities, including degradation of xenobiotics and heavy metal resistance. Although the genomes of several strains of this species have been sequenced, no plasmid has yet been constructed for genetic engineering in this species. In this study, we identifie...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Zhang MN,Bai NL,Ding HT,Zhu XF,Zhao YH

更新日期：2017-02-01 00:00:00

abstract：:Recombinant adeno-associated virus (rAAV) vectors are increasingly popular tools for gene therapy applications. Their non-pathogenic status, low inflammatory potential, availability of viral serotypes with different tissue tropisms, and prospective long-lasting gene expression are important attributes that make rAAVs ...

journal_title：Applied microbiology and biotechnology

authors： Aponte-Ubillus JJ,Barajas D,Peltier J,Bardliving C,Shamlou P,Gold D

更新日期：2018-02-01 00:00:00

abstract：:In recent years, a number of studies have been done on the physiological effects of water-soluble polysaccharides (WSPS) and their antitumor and immuno-enhancing properties. Many edible mushrooms, in particular those rich in WSPS, not only taste good but also contain ingredients beneficial to the physiology of the hum...

journal_title：Applied microbiology and biotechnology

authors： Wang JC,Hu SH,Liang ZC,Yeh CJ

更新日期：2005-06-01 00:00:00

abstract：:Aerobic granules are cultivated by a single bacterial strain, Acinetobacter calcoaceticus, in a sequencing batch reactor (SBR). This strain presents as a good phenol reducer and an efficient auto coagulator in the presence of phenol, mediated by heat-sensitive adhesins proteins. Stable 2.3-mm granules were formed in t...

journal_title：Applied microbiology and biotechnology

authors： Adav SS,Lee DJ

更新日期：2008-03-01 00:00:00

abstract：:River-bay system is a transitional zone connecting land and ocean and an important natural source for methane emission. Methanogens play important roles in the global greenhouse gas budget and carbon cycle since they produce methane. The abundance and community assemblage of methanogens in such a dynamic system are no...

journal_title：Applied microbiology and biotechnology

authors： Zhang CJ,Chen YL,Pan J,Wang YM,Li M

更新日期：2020-05-01 00:00:00

abstract：:Streptolydigin, a secondary metabolite produced by Streptomyces lydicus, is a potent inhibitor of bacterial RNA polymerases. It has been suggested that streptolydigin biosynthesis is associated with polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS). Thus, there is great interest in understanding the...

journal_title：Applied microbiology and biotechnology

authors： Zhao GR,Luo T,Zhou YJ,Jiang X,Qiao B,Yu FM,Yuan YJ

更新日期：2009-05-01 00:00:00

abstract：:Many different organic compounds may be converted by microbial biotransformation to high-value products for the chemical and pharmaceutical industries. This review summarizes the use of strains of Aspergillus niger, a well-known filamentous fungus used in numerous biotechnological processes, for biochemical transforma...

journal_title：Applied microbiology and biotechnology

authors： Parshikov IA,Woodling KA,Sutherland JB

更新日期：2015-09-01 00:00:00