The peculiarities of succinic acid production from rapeseed oil by Yarrowia lipolytica yeast.

abstract：:Transaminase enzymes (TAms) are becoming increasingly valuable in the chemist's toolbox as a biocatalytic route to chiral amines. Despite high profile successes, the lack of (R)-selective TAms and robustness under harsh industrial conditions continue to prove problematic. Herein, we report the isolation of the first h...

journal_title：Applied microbiology and biotechnology

authors： Kelly SA,Magill DJ,Megaw J,Skvortsov T,Allers T,McGrath JW,Allen CCR,Moody TS,Gilmore BF

更新日期：2019-07-01 00:00:00

abstract：:A simple and stable immobilization of a laccase from Pleurotus ostreatus was obtained through genetic fusion with a self-assembling and adhesive class I hydrophobin. The chimera protein was expressed in Pichia pastoris and secreted into the culture medium. The crude culture supernatant was directly used for coatings o...

journal_title：Applied microbiology and biotechnology

authors： Sorrentino I,Giardina P,Piscitelli A

更新日期：2019-04-01 00:00:00

abstract：:The aim of the present study was to develop a new cell modification method to facilitate the cell separation from broth. In order to reduce the transfer limitation of substrate and product caused by general immobilization methods in the following biotransformation of glycerol, the carboxyl-functioned superparamagnetic...

journal_title：Applied microbiology and biotechnology

authors： Chen G,Chen J

更新日期：2013-05-01 00:00:00

abstract：:Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

journal_title：Applied microbiology and biotechnology

authors： Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

更新日期：2006-10-01 00:00:00

abstract：:Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). No CA16 vaccine candidates have progressed to clinical trials so far. Immunogenicity studies indicated that different CA16 particles have much influence on the efficacy of a candidate vaccine. However, there are sti...

journal_title：Applied microbiology and biotechnology

authors： Ye X,Yang L,Jia J,Han J,Li S,Liu Y,Xu L,Zhao H,Chen Y,Li Y,Cheng T,Xia N

更新日期：2016-03-01 00:00:00

abstract：:A new pmoA gene-based PCR primer set was designed for detection of nitrite-dependent anaerobic oxidation of methane (n-damo) bacteria from four different ecosystems, namely rice paddy soil, freshwater reservoir, reed bed, and sludge from wastewater treatment plant. This primer set showed high specificity and efficienc...

journal_title：Applied microbiology and biotechnology

authors： Han P,Gu JD

更新日期：2013-12-01 00:00:00

abstract：:Lactobacillus plantarum MA2 was isolated from traditional Chinese Tibet kefir grains, which possess several excellent properties and functions. We previously demonstrated the antioxidant activities of this bacterium in vitro. However, the maintenance and survival of L. plantarum MA2 inside the murine intestinal tract,...

journal_title：Applied microbiology and biotechnology

authors： Tang W,Xing Z,Hu W,Li C,Wang J,Wang Y

更新日期：2016-08-01 00:00:00

abstract：:In this study, a high yield production bioprocess with recombinant Bacillus megaterium for the production of the extracellular enzyme levansucrase (SacB) was developed. For basic optimization of culture parameters and nutrients, a recombinant B. megaterium reporter strain that produced green fluorescent protein under ...

journal_title：Applied microbiology and biotechnology

authors： Korneli C,Biedendieck R,David F,Jahn D,Wittmann C

更新日期：2013-04-01 00:00:00

abstract：:In the Candida antarctica lipase B-catalyzed hydrolysis of (R,S)-azolides derived from (R,S)-N-protected proline in water-saturated methyl tert-butyl ether (MTBE), high enzyme activity with excellent enantioselectivity (V (S) V (R) ...

journal_title：Applied microbiology and biotechnology

authors： Wu CH,Pen CW,Wang PY,Tsai SW

更新日期：2013-02-01 00:00:00

abstract：:Mannitol is a natural polyol extensively used in the food industry as low-calorie sugar being applicable for diabetic food products. We aimed to evaluate mannitol production by Lactobacillus reuteri CRL 1101 using sugarcane molasses as low-cost energy source. Mannitol formation was studied in free-pH batch cultures us...

journal_title：Applied microbiology and biotechnology

authors： Ortiz ME,Fornaguera MJ,Raya RR,Mozzi F

更新日期：2012-08-01 00:00:00

abstract：:A new medium supplement mixture, PPRF92, has been developed to enable the serial subculture of human diploid fibroblasts (MRC-5 cells) on microcarriers. Furthermore, the PPRF92 supplements enable cell growth at serum levels as low as 1%. Through an optimization programme, the PPRF92 supplements have evolved into a sim...

journal_title：Applied microbiology and biotechnology

authors： Forestell SP,Kalogerakis N,Behie LA

更新日期：1992-11-01 00:00:00

abstract：:A precise and scarless genome excision method, employing the Cre/loxP system in concert with double-strand break (DSB)-stimulated intramolecular recombination was developed. The DSBs were mediated by the restriction endonuclease, I-SceI. It permitted multiple deletions of independent 14-, 43-, and 10-kb-long genomic r...

journal_title：Applied microbiology and biotechnology

authors： Suzuki N,Nonaka H,Tsuge Y,Okayama S,Inui M,Yukawa H

更新日期：2005-11-01 00:00:00

abstract：:Biochemical processes in biogas plants are still not fully understood. Especially, the identification of possible bottlenecks in the complex fermentation processes during biogas production might provide potential to increase the performance of biogas plants. To shed light on the question which group of organism consti...

journal_title：Applied microbiology and biotechnology

authors： Refai S,Wassmann K,Deppenmeier U

更新日期：2014-08-01 00:00:00

abstract：:Dengue virus (DENV) infection is one of the most important public health threats globally; however, no vaccines or effective antivirals are currently available. The bioluminescence imaging technique has emerged as a powerful tool for studies on viral pathogenesis in vitro and in vivo. In this study, using a recombinan...

journal_title：Applied microbiology and biotechnology

authors： Li XF,Deng YQ,Zhao H,Ye Q,Wang HJ,Li SH,Zhu SY,Shi PY,Qin ED,Zhang B,Qin CF

更新日期：2013-05-01 00:00:00

abstract：:Maltol derivatives are utilized in a variety of fields due to their metal-chelating abilities, and modification of the 2-methyl side chain is known to effectively expand their functional diversity. In the present study, microbial enzymes were screened for hydroxylating activity towards the 2-methyl group in a maltol d...

journal_title：Applied microbiology and biotechnology

authors： Kozono I,Mihara K,Minagawa K,Hibi M,Ogawa J

更新日期：2017-09-01 00:00:00

abstract：:The human granulocyte macrophage colony-stimulating factor (GM-CSF) is a glycoprotein with important clinical applications for the treatment of neutropenia and aplastic anemia and reducing infections associated with bone marrow transplants. We evaluated the potential for using a potato virus X (PVX) viral vector syste...

journal_title：Applied microbiology and biotechnology

authors： Zhou F,Wang ML,Albert HH,Moore PH,Zhu YJ

更新日期：2006-10-01 00:00:00

abstract：:The saprotrophic fungus Penicillium griseofulvum was chosen as model organism to study responses to a mixture of hexachlorocyclohexane (HCH) isomers (α-HCH, β-HCH, γ-HCH, δ-HCH) and potentially toxic metals (vanadium, lead) in solid and liquid media. The P. griseofulvum FBL 500 strain was isolated from polluted soil c...

journal_title：Applied microbiology and biotechnology

authors： Ceci A,Pinzari F,Riccardi C,Maggi O,Pierro L,Petrangeli Papini M,Gadd GM,Persiani AM

更新日期：2018-01-01 00:00:00

abstract：:The genome of the coprophilous fungus Podospora anserina displays an impressive array of genes encoding hemicellulolytic enzymes. In this study, we focused on a putative carbohydrate esterase (CE) from family 16 (CE16) that bears a carbohydrate-binding module from family CBM1. The protein was heterologously expressed ...

journal_title：Applied microbiology and biotechnology

authors： Puchart V,Berrin JG,Haon M,Biely P

更新日期：2015-12-01 00:00:00

abstract：:Dengue viruses are emerging mosquito-borne pathogens belonging to Flaviviridae family which are transmitted to humans via the bites of infected mosquitoes Aedes aegypti and Aedes albopictus. Because of the wide distribution of these mosquito vectors, more than 2.5 billion people are approximately at risk of dengue inf...

journal_title：Applied microbiology and biotechnology

authors： Fahimi H,Mohammadipour M,Haddad Kashani H,Parvini F,Sadeghizadeh M

更新日期：2018-04-01 00:00:00

abstract：:Extracellular polymeric substance (EPS) is proposed to facilitate calcium ion supersaturation through its nucleation effect during the microbially induced calcium carbonate precipitation (MICP) process. However, the supersaturation effect of Ca2+ via EPS in MICP has not been clearly demonstrated. Enhanced exopolysacch...

journal_title：Applied microbiology and biotechnology

authors： Kim HJ,Shin B,Lee YS,Park W

更新日期：2017-08-01 00:00:00

abstract：:The environmentally widespread micropollutants bisphenol A (BPA), carbamazepine (CBZ), 17α-ethinylestradiol (EE2), diclofenac (DF), sulfamethoxazole (SMX), technical nonylphenol (t-NP) and triclosan (TCS) were used to assess the potential of the laccase-producing freshwater ascomycete Phoma sp. strain UHH 5-1-03 for m...

journal_title：Applied microbiology and biotechnology

authors： Hofmann U,Schlosser D

更新日期：2016-03-01 00:00:00

abstract：:The objective of this study was to evaluate the effect of an embedded bio-nano hybrid system using nanoscale zinc oxide (n-ZnO) and lindane-degrading yeast Candida VITJzN04 for lindane degradation. Nano-embedding of the yeast was done with chemically synthesized n-ZnO particles (50 mg/mL) and was visualized by atomic ...

journal_title：Applied microbiology and biotechnology

authors： Salam JA,Das N

更新日期：2015-03-01 00:00:00

abstract：:The medicinal mushroom Agaricus blazei produced high amounts of laccase (up to 5,000 units l(-1)) in a complex, agitated liquid medium based on tomato juice, while only traces of the enzyme (<100 units l(-1)) were detected in synthetic glucose-based medium. Purification of the enzyme required three chromatographic ste...

journal_title：Applied microbiology and biotechnology

authors： Ullrich R,Huong le M,Dung NL,Hofrichter M

更新日期：2005-05-01 00:00:00

abstract：:A widespread and hitherto by far underexploited potential among ecologically diverse fungi to pretreat wheat straw and digestate from maize silage in the future perspective of using such lignocellulosic feedstock for fermentative bioenergy production was inferred from a screening of nine freshwater ascomycetes, 76 iso...

journal_title：Applied microbiology and biotechnology

authors： Singh S,Harms H,Schlosser D

更新日期：2014-04-01 00:00:00

abstract：:Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S...

journal_title：Applied microbiology and biotechnology

authors： Bräu B,Hilgenfeld R,Schlingmann M,Marquardt R,Birr E,Wohlleben W,Aufderheide K,Pühler A

更新日期：1991-01-01 00:00:00

abstract：:Whey, the main by-product of the cheese industry, is considered as an important pollutant due to its high chemical and biological oxygen demand. Whey, often considered as waste, has high nutritional value and can be used to obtain value-added products, although some of them need expensive enzymatic synthesis. An econo...

journal_title：Applied microbiology and biotechnology

authors： Pescuma M,de Valdez GF,Mozzi F

更新日期：2015-08-01 00:00:00

abstract：:The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes...

journal_title：Applied microbiology and biotechnology

authors： Jin FJ,Han P,Zhuang M,Zhang ZM,Jin L,Koyama Y

更新日期：2018-01-01 00:00:00

abstract：:Many unicellular microorganisms use small signaling molecules to determine their local concentration. The processes involved in the production and recognition of these signals are collectively known as quorum sensing (QS). This form of cell-cell communication is used by unicellular microorganisms to co-ordinate their ...

journal_title：Applied microbiology and biotechnology

authors： Choudhary S,Schmidt-Dannert C

更新日期：2010-05-01 00:00:00

abstract：:Due to the increasing demand for recombinant proteins, the interest in mammalian cell culture, especially of Chinese hamster ovary cells, grows rapidly. This is accompanied by the desire to improve cell lines in order to achieve higher titers and a better product quality. Until recently, most cell line development pro...

journal_title：Applied microbiology and biotechnology

authors： Krämer O,Klausing S,Noll T

更新日期：2010-09-01 00:00:00

abstract：:A gratuitous strain was developed by disrupting the GAL1 gene (galactokinase) of recombinant Saccharomyces cerevisiae harboring the antithrombotic hirudin gene in the chromosome under the control of the GAL10 promoter. A series of glucose-limited fed-batch cultures were carried out to examine the effects of glucose su...

journal_title：Applied microbiology and biotechnology

authors： Kim MD,Lee TH,Lim HK,Seo JH

更新日期：2004-08-01 00:00:00