Efficient transient expression of human GM-CSF protein in Nicotiana benthamiana using potato virus X vector.

abstract：:A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M....

journal_title：Applied microbiology and biotechnology

authors： Li Z,Geng M,Yang H

更新日期：2015-01-01 00:00:00

abstract：:1,3-Propanediol (1,3-PD) production by fermentation of glycerol was described in 1881 but little attention was paid to this microbial route for over a century. Glycerol conversion to 1,3-PD can be carried out by Clostridia as well as Enterobacteriaceae. The main intermediate of the oxidative pathway is pyruvate, the f...

journal_title：Applied microbiology and biotechnology

authors： Biebl H,Menzel K,Zeng AP,Deckwer WD

更新日期：1999-09-01 00:00:00

abstract：:One of the major aims of bioprocess engineering is the real-time monitoring of important process variables. This is the basis of precise process control and is essential for high productivity as well as the exact documentation of the overall production process. Infrared spectroscopy is a powerful analytical technique ...

journal_title：Applied microbiology and biotechnology

authors： Landgrebe D,Haake C,Höpfner T,Beutel S,Hitzmann B,Scheper T,Rhiel M,Reardon KF

更新日期：2010-09-01 00:00:00

abstract：:Pimaricin (natamycin) is a small polyene macrolide antibiotic used worldwide. This efficient antimycotic and antiprotozoal agent, produced by several soil bacterial species of the genus Streptomyces, has found application in human therapy, in the food and beverage industries and as pesticide. It displays a broad spect...

journal_title：Applied microbiology and biotechnology

authors： Aparicio JF,Barreales EG,Payero TD,Vicente CM,de Pedro A,Santos-Aberturas J

更新日期：2016-01-01 00:00:00

abstract：:Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). No CA16 vaccine candidates have progressed to clinical trials so far. Immunogenicity studies indicated that different CA16 particles have much influence on the efficacy of a candidate vaccine. However, there are sti...

journal_title：Applied microbiology and biotechnology

authors： Ye X,Yang L,Jia J,Han J,Li S,Liu Y,Xu L,Zhao H,Chen Y,Li Y,Cheng T,Xia N

更新日期：2016-03-01 00:00:00

abstract：:For the fermentative production of plant-specific flavanones (naringenin, pinocembrin) by Escherichia coli, a plasmid was constructed which carried an artificial biosynthetic gene cluster, including PAL encoding a phenylalanine ammonia-lyase from a yeast, ScCCL encoding a cinnamate/coumarate:CoA ligase from the actino...

journal_title：Applied microbiology and biotechnology

authors： Miyahisa I,Kaneko M,Funa N,Kawasaki H,Kojima H,Ohnishi Y,Horinouchi S

更新日期：2005-09-01 00:00:00

abstract：:A novel and efficient screening method for pyrroloquinoline quinone (PQQ) high-yielding methylotrophic strains was developed by using glucose dehydrogenase apoenzyme (GDHA) which depended on PQQ as the cofactor. Using this high-throughput method, PQQ high-yielding strains were rapidly screened out from thousands of me...

journal_title：Applied microbiology and biotechnology

authors： Si Z,Zhu J,Wang W,Huang L,Wei P,Cai J,Xu Z

更新日期：2016-12-01 00:00:00

abstract：:Wastewater treatment plants (WWTPs) are major collection pools of antibiotics of which low concentrations may induce antibiotic resistance in their microbial communities and pose threat to human health. However, information is still limited on the microbial community alteration in WWTPs upon exposure to low-dose antib...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Fan C,Low A,He J

更新日期：2014-03-01 00:00:00

abstract：:Streptomyces viridochromogenes Tü494 produces the antibiotic phosphinothricyl-alanyl-alanine (Ptt). Ptt-non-producing mutants were isolated following N-methyl-N'-nitro-N-nitrosoguanidine (NTG) or UV light treatment of spore suspensions. In co-synthesis and bioconversion experiments the mutational blocks in the biosynt...

journal_title：Applied microbiology and biotechnology

authors： Alijah R,Dorendorf J,Talay S,Pühler A,Wohlleben W

更新日期：1991-03-01 00:00:00

abstract：:A 2-l (1-l working volume) two-phase partitioning bioreactor (TPPB) was used as an integrated scrubber/bioreactor in which the removal and destruction of benzene from a gas stream was achieved by the reactor's organic/aqueous liquid contents. The organic solvent used to trap benzene was n-hexadecane, and degradation o...

journal_title：Applied microbiology and biotechnology

authors： Davidson CT,Daugulis AJ

更新日期：2003-08-01 00:00:00

abstract：:The saprotrophic fungus Penicillium griseofulvum was chosen as model organism to study responses to a mixture of hexachlorocyclohexane (HCH) isomers (α-HCH, β-HCH, γ-HCH, δ-HCH) and potentially toxic metals (vanadium, lead) in solid and liquid media. The P. griseofulvum FBL 500 strain was isolated from polluted soil c...

journal_title：Applied microbiology and biotechnology

authors： Ceci A,Pinzari F,Riccardi C,Maggi O,Pierro L,Petrangeli Papini M,Gadd GM,Persiani AM

更新日期：2018-01-01 00:00:00

abstract：:Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A s...

journal_title：Applied microbiology and biotechnology

authors： Morikawa M,Daido H,Pongpobpibool S,Imanaka T

更新日期：1994-11-01 00:00:00

abstract：:κ-Carrageenases exhibit apparent distinctions in gene sequence, molecular weight, enzyme properties, and posttranslational processes. In this study, a new κ-carrageenase gene named cgkZ was cloned from the marine bacterium Zobellia sp. ZM-2. The gene comprised an open reading frame of 1,638 bp and encoded 545 amino ac...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Li G,Mo Z,Mou H

更新日期：2013-12-01 00:00:00

abstract：:The operational temperature of microbial fuel cell reactors influences biofilm development, and this has an impact on anodic biocatalytic activity. In this study, we compared three microbial fuel cell (MFC) reactors acclimated at 10°C, 20°C and 35°C to investigate the effect on biomass development, methanogenesis and ...

journal_title：Applied microbiology and biotechnology

authors： Michie IS,Kim JR,Dinsdale RM,Guwy AJ,Premier GC

更新日期：2011-10-01 00:00:00

abstract：:Biosurfactant rhamnolipids have been claimed to show biological activities of inhibiting the proliferation of cancer cells. In this study, the cytotoxicity of rhamnolipids was examined on four cancer cells (HepG2, Caco-2, Hela, MCF-7 cells) and two normal cells (HK-2 cell, primary hepatocyte). Interestingly, both canc...

journal_title：Applied microbiology and biotechnology

authors： Jiang L,Shen C,Long X,Zhang G,Meng Q

更新日期：2014-12-01 00:00:00

abstract：:Biofilms in drinking water distribution systems (DWDSs) could cause several types of problems, such as the deterioration of water quality, corrosion of pipe walls, and potential proliferation of opportunistic pathogens. In this study, ten biofilm samples from different pipe materials, including ductile cast iron pipe ...

journal_title：Applied microbiology and biotechnology

authors： Ren H,Wang W,Liu Y,Liu S,Lou L,Cheng D,He X,Zhou X,Qiu S,Fu L,Liu J,Hu B

更新日期：2015-12-01 00:00:00

abstract：:In order to enhance the removal efficiency of chemical oxygen demand (COD) in the high-salinity landfill leachate, the dominant strains were isolated from high-salinity landfill leachate. The dominant strains and bacteria consortium were screened for COD treatment potential using an aerobic COD concentration decrease ...

journal_title：Applied microbiology and biotechnology

authors： Guo L,Ji M,Dong H,Wei Y

更新日期：2009-08-01 00:00:00

abstract：:Poly(butanediol succinate) (PBS), a biodegradable polymer, was used as both solid carbon source and biofilm carrier for biological nitrate removal process, in which PBS was filled in a packed-bed bioreactor. The denitrification performance and the microbial diversity of biofilm attached on the surface of PBS were inve...

journal_title：Applied microbiology and biotechnology

authors： Shen Z,Yin Y,Wang J

更新日期：2016-07-01 00:00:00

abstract：:Natural phenazines are versatile secondary metabolites that are mainly produced by Pseudomonas and Streptomyces. All phenazine-type metabolites originate from two precursors: phenazine-1-carboxylic acid (PCA) in Pseudomonas or phenazine-1,6-dicarboxylic acid (PDC) in Streptomyces and other bacteria. Although the biosy...

journal_title：Applied microbiology and biotechnology

authors： Guo S,Wang Y,Dai B,Wang W,Hu H,Huang X,Zhang X

更新日期：2017-10-01 00:00:00

abstract：:Streptomyces lividans IAF18, obtained by homologous cloning, is capable of over-producing XlnA. To investigate the possibility of the expression of foreign genes, various coding regions of the xylanase A gene (xlnA) were analysed. Expression/secretion vectors were constructed containing the regulatory elements of xlnA...

journal_title：Applied microbiology and biotechnology

authors： Paradis FW,Shareck F,Dupont C,Kluepfel D,Morosoli R

更新日期：1996-06-01 00:00:00

abstract：:A 7.3-kDa antifungal peptide with an N-terminal sequence exhibiting remarkable homology to defensins from other leguminous plants was isolated from Phaseolus vulgaris cv. 'Spotted Bean'. The isolation procedure involved ion exchange chromatography on O-diethylaminoethyl (DEAE) cellulose, affinity chromatography on Aff...

journal_title：Applied microbiology and biotechnology

authors： Wang HX,Ng TB

更新日期：2007-02-01 00:00:00

abstract：:Cyclodipeptide oxidases (CDOs) perform dehydrogenations on diketopiperazines and play an important role in the cyclodipeptide diversification. In this study, we investigated the two known CDOs AlbA/B and Ndas_1146/7 and one new member, CDO-Np. LC-MS monitoring of 32 cyclodipeptide biotransformations in E. coli reveale...

journal_title：Applied microbiology and biotechnology

authors： Mikulski L,Schäfer J,Brockmeyer K,Kraut R,Li SM

更新日期：2020-03-01 00:00:00

abstract：:The six- and seven-subunit Na+/H+ antiporters (Mrp) are widely distributed in bacteria. They are reported to be integral for pH homeostasis in alkaliphilic bacteria when adapting to high pH environments. In this study, operons encoding for the six-subunit Na+/H+ antiporters were found in the genomes of all studied Die...

journal_title：Applied microbiology and biotechnology

authors： Fang H,Qin XY,Zhang KD,Nie Y,Wu XL

更新日期：2018-04-01 00:00:00

abstract：:In order for microalgae-based processes to reach commercial production for biofuels and high-value products such as omega-3 fatty acids, it is necessary that economic feasibility be demonstrated at the industrial scale. Therefore, process optimization is critical to ensure that the maximum yield can be achieved from t...

journal_title：Applied microbiology and biotechnology

authors： Lowrey J,Armenta RE,Brooks MS

更新日期：2016-02-01 00:00:00

abstract：:α-Galacto-oligosaccharides (α-GOS) are produced by transgalactosylation reactions of α-galactosidase (α-Gal) or by conversion of raffinose family oligosaccharides by levansucrase. Similarly to β-GOS, α-GOS have the potential to mimic glycan receptors on eukaryotic cells and act as molecular decoys to prevent bacterial...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Black BA,Curtis JM,Gänzle MG

更新日期：2014-03-01 00:00:00

abstract：:Photothermal cancer therapy, as a prospective approach for local cancer treatment, is attracting increasing interests. In this paper, gold nanorods were conjugated with folate (folate/AuNRs), and their photothermal effects on hepatocellular carcinoma cell line (HepG2) using MTT assay, flow cytometry, as well as on the...

journal_title：Applied microbiology and biotechnology

authors： Jin H,Yang P,Cai J,Wang J,Liu M

更新日期：2012-06-01 00:00:00

abstract：:Environmental research delivers valuable bacterial resources for biotechnology. We believe that systematic long-term preservation of bacteria will promote future biotechnological innovations, by safeguarding the accessibility of bacteria already recognized to have interesting features and providing a "pool" of bacteri...

journal_title：Applied microbiology and biotechnology

authors： Heylen K,Hoefman S,Vekeman B,Peiren J,De Vos P

更新日期：2012-05-01 00:00:00

abstract：:Three bacterial strains utilizing paracetamol as the sole carbon, nitrogen, and energy source were isolated from a paracetamol-degrading aerobic aggregate, and assigned to species of the genera Stenotrophomonas and Pseudomonas. The Stenotrophomonas species have not included any known paracetamol degraders until now. I...

journal_title：Applied microbiology and biotechnology

authors： Zhang L,Hu J,Zhu R,Zhou Q,Chen J

更新日期：2013-04-01 00:00:00

abstract：:Bionanocapsules (BNCs) are hollow nanoscale particles composed of L protein of the hepatitis B virus surface antigen that represent specific affinity for human hepatocytes. BNCs can transfer genes and drugs into human hepatocytes efficiently and specifically. BNC can be expressed in yeast cells. In this study, we deve...

journal_title：Applied microbiology and biotechnology

authors： Shishido T,Muraoka M,Ueda M,Seno M,Tanizawa K,Kuroda S,Fukuda H,Kondo A

更新日期：2006-12-01 00:00:00

abstract：:Based on our previous study evaluating the in vivo cure efficacy of chitosan on bovine mastitis, a more water-soluble chitosan-oligosaccharide (OCHT) with a high degree of deacetylation and low molecular weight was prepared to obtain high antibiotic efficacy. The growth of Staphylococcus aureus isolated from bovine ma...

journal_title：Applied microbiology and biotechnology

authors： Moon JS,Kim HK,Koo HC,Joo YS,Nam HM,Park YH,Kang MI

更新日期：2007-07-01 00:00:00