Biotechnological production and application of the antibiotic pimaricin: biosynthesis and its regulation.

abstract：:Soil metagenome constitutes a reservoir for discovering novel enzymes from the unculturable microbial diversity. From three plant rhizosphere metagenomic libraries comprising a total of 142,900 members of recombinant plasmids, we obtained 14 recombinant fosmids that exhibited lipolytic activity. A selected recombinant...

journal_title：Applied microbiology and biotechnology

authors： Lee MH,Hong KS,Malhotra S,Park JH,Hwang EC,Choi HK,Kim YS,Tao W,Lee SW

更新日期：2010-11-01 00:00:00

abstract：:Two-dimensional gel electrophoresis was conducted to investigate the effect of H2O2 on whole protein expression in Acinetobacter oleivorans DR1. Functional classification of 13 upregulated proteins using MALDI-TOF mass spectrometry showed relationships with oxidative stress, energy production and conversion, nucleotid...

journal_title：Applied microbiology and biotechnology

authors： Kim J,Cho Y,Jang IA,Park W

更新日期：2015-12-01 00:00:00

abstract：:Rhamnolipids produced by Pseudomonas aeruginosa are biosurfactants with a high biotechnological potential, but their extensive commercialization is limited by the potential virulence of P. aeruginosa and by restrictions in producing these surfactants in heterologous hosts. In this work, we report the characterization ...

journal_title：Applied microbiology and biotechnology

authors： Grosso-Becerra MV,González-Valdez A,Granados-Martínez MJ,Morales E,Servín-González L,Méndez JL,Delgado G,Morales-Espinosa R,Ponce-Soto GY,Cocotl-Yañez M,Soberón-Chávez G

更新日期：2016-12-01 00:00:00

abstract：:Biofilm development in methanogenic fluidized-bed reactors with sand as the carrier was studied on a laboratory scale. The microorganisms present in consecutive layers of the biofilm of mature sludge granules were preliminarily characterized on the basis of their morphology, element composition and adhesion capacity a...

journal_title：Applied microbiology and biotechnology

authors： Lauwers AM,Heinen W,Gorris LG,van der Drift C

更新日期：1990-06-01 00:00:00

abstract：:Confocal Raman microspectral imaging (CRMI) is an advanced cell-imaging method that maps endogenous molecular compositions with their unique spectral fingerprint indicators. The aim of this work was to provide a visualized understanding of subcellular features of live osteosarcoma cells using a 532-nm laser excitation...

journal_title：Applied microbiology and biotechnology

authors： Li J,Qin J,Zhang X,Wang R,Liang Z,He Q,Wang Z,Wang K,Wang S

更新日期：2019-08-01 00:00:00

abstract：:The Escherichia coli (E. coli) expression system has been widely used to produce recombinant proteins. However, in some heterologous expressions, there are still difficulties in large-scale production. The use of fusion partners is one of the strategies for improving the expression levels of proteins in E. coli host. ...

journal_title：Applied microbiology and biotechnology

authors： Nguyen TKM,Ki MR,Son RG,Pack SP

更新日期：2019-03-01 00:00:00

abstract：:Traditional temperature-sensitive systems use either heat shock (40-42 °C) or cold shock (15-23 °C) to induce gene expression at temperatures that are not the optimal temperature for host cell growth (37 °C). This impacts the overall productivity and yield by disturbing cell growth and cellular metabolism. Here, we ha...

journal_title：Applied microbiology and biotechnology

authors： Jiang X,Zhang H,Yang J,Liu M,Feng H,Liu X,Cao Y,Feng D,Xian M

更新日期：2013-06-01 00:00:00

abstract：:Two hypothetical proteins XP_001818628 and XP_001819091 (designated AoFaeB and AoFaeC, respectively), showing sequence identity with known type-C feruloyl esterases, have been found in the genomic sequence of Aspergillus oryzae. We cloned the putative A. oryzae feruloyl esterase-encoding genes and expressed them in Pi...

journal_title：Applied microbiology and biotechnology

authors： Koseki T,Hori A,Seki S,Murayama T,Shiono Y

更新日期：2009-06-01 00:00:00

abstract：:A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction condi...

journal_title：Applied microbiology and biotechnology

authors： Seo MH,Kim KR,Oh DK

更新日期：2013-10-01 00:00:00

abstract：:1,3-propanediol (1,3-PD) was produced with a robust fermentation process using waste glycerol feedstock from biodiesel production and a soil-based bacterial inoculum. An iterative inoculation method was developed to achieve independence from soil and selectively breed bacterial populations capable of glycerol metaboli...

journal_title：Applied microbiology and biotechnology

authors： Kanjilal B,Noshadi I,Bautista EJ,Srivastava R,Parnas RS

更新日期：2015-03-01 00:00:00

abstract：:We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was ...

journal_title：Applied microbiology and biotechnology

authors： Tanimura K,Takashima S,Matsumoto T,Tanaka T,Kondo A

更新日期：2016-07-01 00:00:00

abstract：:Cell-free preparations of Tetrahymena thermophila catalyze the direct desaturation of cholesterol to delta7-dehydrocholesterol (provitamin D3). The activity was isolated in the microsomal fraction from Tetrahymena homogenates. Delta7-desaturase activity was stimulated fivefold by the addition of 6 mM ATP. Other cofact...

journal_title：Applied microbiology and biotechnology

authors： Valcarce G,Florin-Christensen J,Nudel C

更新日期：2000-05-01 00:00:00

abstract：:Small ubiquitin-related modifier (SUMO) technology has been widely used in Escherichia coli expression systems to produce antimicrobial peptides. However, E. coli is a pathogenic bacterium that produces endotoxins and can secrete proteins into the periplasm, forming inclusion bodies. In our work, cathelicidin-BF (CBF)...

journal_title：Applied microbiology and biotechnology

authors： Luan C,Zhang HW,Song DG,Xie YG,Feng J,Wang YZ

更新日期：2014-04-01 00:00:00

abstract：:In industry, filamentous fungi have a prominent position as producers of economically relevant primary or secondary metabolites. Particularly, the advent of genetic engineering of filamentous fungi has led to a growing number of molecular tools to adopt filamentous fungi for biotechnical applications. Here, we summari...

journal_title：Applied microbiology and biotechnology

authors： Kluge J,Terfehr D,Kück U

更新日期：2018-08-01 00:00:00

abstract：:Glycosyltransferases (GTs)-mediated glycodiversification studies have drawn significant attention recently, with the goal of generating bioactive compounds with improved pharmacological properties by diversifying the appended sugars. The key to achieving glycodiversification is to identify natural and/or engineered fl...

journal_title：Applied microbiology and biotechnology

authors： Li L,Pan G,Zhu X,Fan K,Gao W,Ai G,Ren J,Shi M,Olano C,Salas JA,Yang K

更新日期：2017-07-01 00:00:00

abstract：:Biosurfactant rhamnolipids have been claimed to show biological activities of inhibiting the proliferation of cancer cells. In this study, the cytotoxicity of rhamnolipids was examined on four cancer cells (HepG2, Caco-2, Hela, MCF-7 cells) and two normal cells (HK-2 cell, primary hepatocyte). Interestingly, both canc...

journal_title：Applied microbiology and biotechnology

authors： Jiang L,Shen C,Long X,Zhang G,Meng Q

更新日期：2014-12-01 00:00:00

abstract：:A new method is proposed for the evaluation of the activity of sourdough strains, based on gas pressure measurements in closed air-tight reactors. Gas pressure and pH were monitored on-line during the cultivation of commercial yeasts and heterofermentative lactic acid bacteria on a semi-synthetic medium with glucose a...

journal_title：Applied microbiology and biotechnology

authors： Wick M,Vanhoutte JJ,Adhemard A,Turini G,Lebeault JM

更新日期：2001-04-01 00:00:00

abstract：:Suspension cultures of the endemic South-African plant Cyclopia subternata were established for the first time and evaluated for the presence of isoflavones. The influence of light, as well as medium supplementation strategies with phenylalanine, casein hydrolysate and coconut water on biomass growth and isoflavone pr...

journal_title：Applied microbiology and biotechnology

authors： Kokotkiewicz A,Luczkiewicz M,Kowalski W,Badura A,Piekus N,Bucinski A

更新日期：2013-10-01 00:00:00

abstract：:Using molecular techniques and sequencing, we studied the intraspecific diversity of Oenococcus oeni, a lactic acid bacterium involved in red winemaking. A relationship between the phenotypic and genotypic characterization of 16 O. oeni strains isolated from wine with different levels of enological potential was shown...

journal_title：Applied microbiology and biotechnology

authors： Delaherche A,Bon E,Dupé A,Lucas M,Arveiler B,De Daruvar A,Lonvaud-Funel A

更新日期：2006-11-01 00:00:00

abstract：:Production of gluconic acid by cells of Aspergillus niger immobilized on polyurethane foam was studied in repeated-batch shake-flask and bubble-column fermentations. For passive immobilization, various amounts of polyurethane foam and spore suspension were tested in order to obtain a suitable combination for optimal c...

journal_title：Applied microbiology and biotechnology

authors： Vassilev NB,Vassileva MC,Spassova DI

更新日期：1993-06-01 00:00:00

abstract：:Pseudomonas putida KT2440 grew on glucose at a specific rate of 0.48 h(-1) but accumulated almost no poly-3-hydroxyalkanoates (PHA). Subsequent nitrogen limitation on nonanoic acid resulted in the accumulation of only 27% medium-chain-length PHA (MCL-PHA). In contrast, exponential nonanoic acid-limited growth (mu = 0....

journal_title：Applied microbiology and biotechnology

authors： Sun Z,Ramsay JA,Guay M,Ramsay BA

更新日期：2007-02-01 00:00:00

abstract：:Recombinant production of mammalian cytoplasmic proteins plays a major role in developing pharmaceutical products. Here we describe two expression technologies using unique nature of halophilic bacteria. One of such properties of halophilic bacteria is accumulation of compatible solutes in the cytoplasm. As the compat...

journal_title：Applied microbiology and biotechnology

authors： Tokunaga H,Arakawa T,Tokunaga M

更新日期：2010-12-01 00:00:00

abstract：:Fatty acids are a promising raw material for substance production because of their highly reduced and anhydrous nature, which can provide higher fermentation yields than sugars. However, they are insoluble in water and are poorly utilized by microbes in industrial fermentation production. We used fatty acids as raw ma...

journal_title：Applied microbiology and biotechnology

authors： Doi H,Hoshino Y,Nakase K,Usuda Y

更新日期：2014-01-01 00:00:00

abstract：:Despite the known hazardous effects of antimony (Sb) on human health, Sb monitoring biosensors have not been as actively investigated as arsenic (As) biosensors. Whole-cell bioreporters (WCBs) employing an arsenic-responsive operon and a regulatory protein (ArsR) are reportedly capable of monitoring arsenite, arsenate...

journal_title：Applied microbiology and biotechnology

authors： Lee W,Kim H,Jang G,Kim BG,Yoon Y

更新日期：2020-03-01 00:00:00

abstract：:The fungal conversions of sulfur-containing heterocyclic compounds were investigated using the lignin-degrading basidiomycete Coriolus versicolor. The fungus metabolized a series of sulfur compounds--25 structurally related thiophene derivatives--via several different pathways. Under primary metabolic conditions, C. v...

journal_title：Applied microbiology and biotechnology

authors： Ichinose H,Nakamizo M,Wariishi H,Tanaka H

更新日期：2002-03-01 00:00:00

abstract：:Two bacterial strains, Ralstonia sp. LD35 and Pseudomonas putida DSM 1868, were assayed for their ability to degrade the monocyclic aromatic compounds commonly found in olive mill wastewaters (OMWs). The goal was to study the possibility of employing the two strains in the removal of these recalcitrant and toxic compo...

journal_title：Applied microbiology and biotechnology

authors： Di Gioia D,Fava F,Bertin L,Marchetti L

更新日期：2001-05-01 00:00:00

abstract：:The effects of pH control strategy and fermentative operation modes on the biosynthesis of pyrroloquinoline quinine (PQQ) were investigated systematically with Methylobacillus sp. CCTCC M2016079 in the present work. Firstly, the shake-flask cultivations and benchtop fermentations at various pH values ranging from 5.3 ...

journal_title：Applied microbiology and biotechnology

authors： Si Z,Machaku D,Wei P,Huang L,Cai J,Xu Z

更新日期：2017-06-01 00:00:00

abstract：:Despite being a yellow pigment visible to the human eye, coelimycin (CPK) remained to be an undiscovered secondary metabolite for over 50 years of Streptomyces research. Although the function of this polyketide is still unclear, we now know that its "cryptic" nature is attributed to a very complex and precise mechanis...

journal_title：Applied microbiology and biotechnology

authors： Bednarz B,Kotowska M,Pawlik KJ

更新日期：2019-08-01 00:00:00

abstract：:A study was conducted to optimize the biodegradation in soil slurries of phenanthrene initially dissolved in nonaqueous-phase liquids (NAPLs). The slow rate of degradation of phenanthrene in dibutyl phthalate was increased by addition of phenanthrene-degrading microorganisms to soil slurries containing the NAPL. The r...

journal_title：Applied microbiology and biotechnology

authors： Birman I,Alexander M

更新日期：1996-03-01 00:00:00

abstract：:The 1,2-propanediol (1,2-PD) inducible membrane-bound L-pantoyl lactone (L-PL) dehydrogenase (LPLDH) has been isolated from Rhodococcus erythropolis AKU2103 (Kataoka et al. in Eur J Biochem 204:799, 1992). Based on the N-terminal amino acid sequence of LPLDH and the highly conserved amino acid sequence in homology sea...

journal_title：Applied microbiology and biotechnology

authors： Si D,Urano N,Nozaki S,Honda K,Shimizu S,Kataoka M

更新日期：2012-07-01 00:00:00