Antimony sensing whole-cell bioreporters derived from ArsR genetic engineering.

abstract：:Extremely acidophilic microorganisms have an optimal pH of <3 and are found in all three domains of life. As metals are more soluble at acid pH, acidophiles are often challenged by very high metal concentrations. Acidophiles are metal-tolerant by both intrinsic, passive mechanisms as well as active systems. Passive me...

journal_title：Applied microbiology and biotechnology

authors： Dopson M,Holmes DS

更新日期：2014-10-01 00:00:00

abstract：:In this study, we investigated the possibility of using a modified hydantoinase process for the production of optically pure β-amino acids. Two aryl-substituted dihydropyrimidines D,L-6-phenyl-5,6-dihydrouracil (PheDU) and para-chloro-D,L-6-phenyl-5,6-dihydrouracil (pClPheDU) were synthesized. Hydrolysis of these nove...

journal_title：Applied microbiology and biotechnology

authors： Engel U,Syldatk C,Rudat J

更新日期：2012-06-01 00:00:00

abstract：:In this work, phytosterol-biotransforming strains were selected from Mycobacterium sp., using a high concentration of beta-sitosterol. The selection was made by culturing the strains in a medium enriched with 14 g beta-sitosterol/l as the unique source of carbon. During 2 months, the bacterial cultures were transferre...

journal_title：Applied microbiology and biotechnology

authors： Vidal M,Becerra J,Mondaca MA,Silva M

更新日期：2001-10-01 00:00:00

abstract：:Proteins that catalyze H2-pathways often contain iron-sulfur (Fe-S) clusters and are sensitive to O2. We tested whether deletion of the gene encoding the transcriptional negative regulator, IscR, could enhance the ability of Escherichia coli BL21 to synthesize active recombinant H2-pathway components and stimulate fer...

journal_title：Applied microbiology and biotechnology

authors： Akhtar MK,Jones PR

更新日期：2008-04-01 00:00:00

abstract：:The mevalonate pathway plays an important role in providing the cell with a number of essential precursors for the synthesis of biomass constituents. With respect to their chemical structure, the metabolites of this pathway can be divided into two groups: acyl esters [acetoacetyl CoA, acetyl CoA, hydroxymethylglutaryl...

journal_title：Applied microbiology and biotechnology

authors： Seker T,Møller K,Nielsen J

更新日期：2005-04-01 00:00:00

abstract：:Genetic engineering of transcription factors is an efficient strategy to improve lignocellulolytic enzyme production in fungi. In this study, the xylanase transcriptional regulators of Trichoderma reesei (Xyr1) and Neurospora crassa (XLR-1), as well as their constitutively active mutants (Xyr1A824V and XLR-1A828V), we...

journal_title：Applied microbiology and biotechnology

authors： Xia C,Li Z,Xu Y,Yang P,Gao L,Yan Q,Li S,Wang Y,Qu Y,Song X

更新日期：2019-03-01 00:00:00

abstract：:L-cysteine, a valuable sulfur-containing amino acid, has been widely used in food, agriculture, and pharmaceutical industries. Due to the toxicity and complex regulation of L-cysteine, no efficient cell factory has yet been achieved for L-cysteine industrial production. In this study, the food-grade microorganism Cory...

journal_title：Applied microbiology and biotechnology

authors： Wei L,Wang H,Xu N,Zhou W,Ju J,Liu J,Ma Y

更新日期：2019-02-01 00:00:00

abstract：:Microalgal starch is a potential feedstock for biofuel production. Nutrient stress is widely used to stimulate starch accumulation in microalgae. Cell growth and starch accumulation in the marine green microalga Tetraselmis subcordiformis were evaluated under extracellular phosphorus deprivation with initial cell dens...

journal_title：Applied microbiology and biotechnology

authors： Yao CH,Ai JN,Cao XP,Xue S

更新日期：2013-07-01 00:00:00

abstract：:Leather biotechnology based on enzyme is one of the main directions toward clean technology in the leather manufacturing process. Proteins such as collagen, elastin, and keratin are important components in animal hides or skins, and proteases are most frequently used in the leather manufacturing process for the remova...

journal_title：Applied microbiology and biotechnology

authors： Li Y,Zhang C,Luo F,Peng B

更新日期：2018-12-01 00:00:00

abstract：:A large percentage of organic fuel consumed in a microbial fuel cell (MFC) is lost as a result of oxygen transfer through the cathode. In order to understand how this oxygen transfer affects the microbial community structure, reactors were operated in duplicate using three configurations: closed circuit (CC; with curr...

journal_title：Applied microbiology and biotechnology

authors： Shehab N,Li D,Amy GL,Logan BE,Saikaly PE

更新日期：2013-11-01 00:00:00

abstract：:This study has investigated the acclimation of ammonia-oxidizing communities (AOC) to low dissolved oxygen (DO) concentrations. Under controlled laboratory conditions, two sequencing batch reactors seeded with activated sludge from the same source were operated at high DO (near saturation) and low DO (0.1 mg O₂/L) con...

journal_title：Applied microbiology and biotechnology

authors： Arnaldos M,Kunkel SA,Stark BC,Pagilla KR

更新日期：2013-12-01 00:00:00

abstract：:The presence of microorganisms in biological fluids like urine and blood is an indication of vulnerability to infections. Iron is one of the important micronutrients required for bacterial growth. In an iron-deficit environment, bacteria release high-affinity iron-chelating compounds called siderophores which can be u...

journal_title：Applied microbiology and biotechnology

authors： Sankaranarayanan R,Alagumaruthanayagam A,Sankaran K

更新日期：2015-03-01 00:00:00

abstract：:Tolypocladium guangdongense is a high-value edible fungus with various medicinal and food safety properties. However, its evolutionary and genetic information is still limited. Mitochondrial genomes are potential models for molecular evolution and phylogenetic studies. In this study, we sequenced the complete mitogeno...

journal_title：Applied microbiology and biotechnology

authors： Zhang C,Dai Y,Wang G,Wang C,Gao Y,Deng W,Li T

更新日期：2020-11-01 00:00:00

abstract：:The demand of renewable energy sources, i.e. biomass, is steadily increasing worldwide to reduce the need of fossil energy sources. Biomass such as energy crops, woody species, forestry and agricultural residues are the most common renewable energy sources. Due to uneven demand for wood fuel, the material is mostly st...

journal_title：Applied microbiology and biotechnology

authors： Noll M,Jirjis R

更新日期：2012-08-01 00:00:00

abstract：:Homologous recombination-based recombineering is a widely used DNA cloning and modification technique; recombineering efficiency improvement would be helpful for high-throughput DNA manipulation. Escherichia coli primase DnaG variants, such as DnaG Q576A and DnaG K580A, increase the recombineering efficiency via impai...

journal_title：Applied microbiology and biotechnology

authors： Li J,Sun J,Gao X,Wu Z,Shang G

更新日期：2019-04-01 00:00:00

abstract：:Problems with deflocculation and solids separation in biological wastewater treatment systems are linked to fluctuations in physicochemical conditions. This study examined the composition of activated sludge bacterial communities in lab-scale sequencing batch reactors treating bleached kraft mill effluent, under trans...

journal_title：Applied microbiology and biotechnology

authors： Nadarajah N,Allen DG,Fulthorpe RR

更新日期：2010-11-01 00:00:00

abstract：:A characteristic mucosal immune response involves the production of antigen-specific secretory immunoglobulin A (SIgA) antibodies. In order to study transcytosis by mimicking the SIgA secretion and to screen for SIgA secretion-promoting substances, we developed a model system of a transfected Madin-Darby canine kidney...

journal_title：Applied microbiology and biotechnology

authors： Zuo T,Feng X,Zhang N,Xue C,Tang QJ

更新日期：2015-07-01 00:00:00

abstract：:The activities of seven hydrolytic enzymes (L-alanine aminopeptidase, esterase, α-and β-glucosidase, phosphomonoesterase, phosphodiesterase, sulfatase) were monitored during 1 year in parallel and serial treatment units of the biological stage of a communal wastewater treatment plant. The spatial homogeneity of enzyme...

journal_title：Applied microbiology and biotechnology

authors： Kreutz JA,Böckenhüser I,Wacht M,Fischer K

更新日期：2016-08-01 00:00:00

abstract：:Mannosylerythritol lipids (MELs) are a type of glycolipid biosurfactant produced by basidiomycetous yeasts, most notably those belonging to the genera Pseudozyma and Ustilago. Mannosylerythritol lipids are environmentally friendly and possess many unique functions, such as gene delivery, bio-activation, and human skin...

journal_title：Applied microbiology and biotechnology

authors： Saika A,Koike H,Fukuoka T,Morita T

更新日期：2018-08-01 00:00:00

abstract：:Mycorrhizal fungi belonging to the genus Tuber produce, after the establishment of a productive interaction with a plant host, hypogeous fruitbodies of great economic value known as ''truffles''. This review summarizes the state of art on life cycle, genetic, and biotechnological investigations of Tuber spp. The ascoc...

journal_title：Applied microbiology and biotechnology

authors： Poma A,Limongi T,Pacioni G

更新日期：2006-09-01 00:00:00

abstract：:Actinobacteria, a large group of Gram-positive bacteria, secrete a wide range of extracellular enzymes involved in the degradation of organic compounds and biopolymers including the ubiquitous aminopolysaccharides chitin and chitosan. While chitinolytic enzymes are distributed in all kingdoms of life, actinobacteria a...

journal_title：Applied microbiology and biotechnology

authors： Lacombe-Harvey MÈ,Brzezinski R,Beaulieu C

更新日期：2018-09-01 00:00:00

abstract：:The ability of taxonomically different yeasts to synthesize pyruvic acid (PA) from glucose was studied. The study showed that many yeasts are able to produce PA from glucose under the condition of growth limitation by thiamine. This ability was found in the yeast Blastobotrys adeninivorans for the first time. The prod...

journal_title：Applied microbiology and biotechnology

authors： Kamzolova SV,Morgunov IG

更新日期：2016-09-01 00:00:00

abstract：:Cell growth needs to be monitored in biological studies and bioprocess optimization. In special circumstances, such as microbial fermentations in media containing insoluble particles, accurate cell growth quantification is a challenge with current methods. Only the Burton method is applicable in such circumstances. Th...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Xiang S,Dai X,Yang K

更新日期：2013-06-01 00:00:00

abstract：:Maltol derivatives are utilized in a variety of fields due to their metal-chelating abilities, and modification of the 2-methyl side chain is known to effectively expand their functional diversity. In the present study, microbial enzymes were screened for hydroxylating activity towards the 2-methyl group in a maltol d...

journal_title：Applied microbiology and biotechnology

authors： Kozono I,Mihara K,Minagawa K,Hibi M,Ogawa J

更新日期：2017-09-01 00:00:00

abstract：:Eight Escherichia coli strains were studied in minimal medium with a continuous flow system using confocal microscopy. K12 wild-type strains ATCC 25404 and MG1655 formed the best biofilms ( approximately 43 microm thick, 21 to 34% surface coverage). JM109, DH5alpha, and MG1655 motA formed intermediate biofilms ( appro...

journal_title：Applied microbiology and biotechnology

authors： Wood TK,González Barrios AF,Herzberg M,Lee J

更新日期：2006-09-01 00:00:00

abstract：:The marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental c...

journal_title：Applied microbiology and biotechnology

authors： Kautharapu KB,Rathmacher J,Jarboe LR

更新日期：2013-04-01 00:00:00

abstract：:Saccharomyces cerevisiae is the organism of choice for many food and beverage fermentations because it thrives in high-sugar and high-ethanol conditions. However, the conditions encountered in bioethanol fermentation pose specific challenges, including extremely high sugar and ethanol concentrations, high temperature,...

journal_title：Applied microbiology and biotechnology

authors： Mukherjee V,Steensels J,Lievens B,Van de Voorde I,Verplaetse A,Aerts G,Willems KA,Thevelein JM,Verstrepen KJ,Ruyters S

更新日期：2014-11-01 00:00:00

abstract：:Dengue virus (DENV) infection is one of the most important public health threats globally; however, no vaccines or effective antivirals are currently available. The bioluminescence imaging technique has emerged as a powerful tool for studies on viral pathogenesis in vitro and in vivo. In this study, using a recombinan...

journal_title：Applied microbiology and biotechnology

authors： Li XF,Deng YQ,Zhao H,Ye Q,Wang HJ,Li SH,Zhu SY,Shi PY,Qin ED,Zhang B,Qin CF

更新日期：2013-05-01 00:00:00

abstract：:Media and growth conditions were optimized for the microaerobic cultivation of Magnetospirillum gryphiswaldense in flasks and in a fermentor, resulting in significantly increased cell and magnetosome yields, compared with earlier studies. A reliable method was established for the automatic control of low dissolved oxy...

journal_title：Applied microbiology and biotechnology

authors： Heyen U,Schüler D

更新日期：2003-06-01 00:00:00

abstract：:A moderately halophilic alkalitolerant Bacillus sp. Strain TSCVKK, with an ability to produce extracellular halophilic, alkalitolerant, surfactant, and detergent-stable alpha-amylase was isolated from soil samples obtained from a salt-manufacturing industry in Chennai. The culture conditions for higher amylase product...

journal_title：Applied microbiology and biotechnology

authors： Kiran KK,Chandra TS

更新日期：2008-01-01 00:00:00