A new approach for quantitative characterization of hydrolytic action of proteases to elastin in leather manufacturing.

abstract：:We describe a novel chromosome engineering technique for shuffling selected regions of chromosomes from two strains in Saccharomyces cerevisiae: The technique starts with the construction of MATa and MATalpha strains in which a particular chromosome is split at exactly the same site in both strains such that the split...

journal_title：Applied microbiology and biotechnology

authors： Sugiyama M,Yamamoto E,Mukai Y,Kaneko Y,Nishizawa M,Harashima S

更新日期：2006-10-01 00:00:00

abstract：:Genetic engineering offers a practical route for enhancing the insect biological control potential of entomopathogenic fungi such as Beauveria bassiana. To date, however, such efforts have relied upon transformation protocols that utilize antibiotic or herbicidal resistance markers as selection agents for the introduc...

journal_title：Applied microbiology and biotechnology

authors： Ying SH,Feng MG,Keyhani NO

更新日期：2013-04-01 00:00:00

abstract：:Natural phenazines are versatile secondary metabolites that are mainly produced by Pseudomonas and Streptomyces. All phenazine-type metabolites originate from two precursors: phenazine-1-carboxylic acid (PCA) in Pseudomonas or phenazine-1,6-dicarboxylic acid (PDC) in Streptomyces and other bacteria. Although the biosy...

journal_title：Applied microbiology and biotechnology

authors： Guo S,Wang Y,Dai B,Wang W,Hu H,Huang X,Zhang X

更新日期：2017-10-01 00:00:00

abstract：:In this study, a high yield production bioprocess with recombinant Bacillus megaterium for the production of the extracellular enzyme levansucrase (SacB) was developed. For basic optimization of culture parameters and nutrients, a recombinant B. megaterium reporter strain that produced green fluorescent protein under ...

journal_title：Applied microbiology and biotechnology

authors： Korneli C,Biedendieck R,David F,Jahn D,Wittmann C

更新日期：2013-04-01 00:00:00

abstract：:Pneumococcal surface protein A (PspA) is essential for Streptococcus pneumoniae virulence and its use either as a novel pneumococcal vaccine or as carrier in a conjugate vaccine would improve the protection and the coverage of the vaccine. Within this context, the development of scalable production and purification pr...

journal_title：Applied microbiology and biotechnology

authors： Carvalho RJ,Cabrera-Crespo J,Tanizaki MM,Gonçalves VM

更新日期：2012-05-01 00:00:00

abstract：:Soluble expression of recombinant therapeutic proteins in Escherichia coli (E. coli) has been a challenging task in biopharmaceutical development. In this study, a novel self-cleavable tag Zbasic-intein has been constructed for the soluble expression and purification of a recombinant cytokine, human interleukin-15 (IL...

journal_title：Applied microbiology and biotechnology

authors： Shi S,Chen H,Jiang H,Xie Y,Zhang L,Li N,Zhu C,Chen J,Luo H,Wang J,Feng L,Lu H,Zhu J

更新日期：2017-02-01 00:00:00

abstract：:The effects of mitomycin C on C1857-controlled recombinant gene expression have been explored in E. coli cultures when the drug was added simultaneously to the thermal induction. A significantly improved yield of homologous, heterologous and chimeric fusion proteins was observed in E. coli MC1061 and GE864 (a MC4100 d...

journal_title：Applied microbiology and biotechnology

authors： Corchero JL,Carbonell X,Benito A,Villaverde A

更新日期：1995-03-01 00:00:00

abstract：:Escherichia coli and Lactobacillus plantarum were subjected to final water potentials of -5.6 MPa and -11.5 MPa with three solutes: glycerol, sorbitol and NaCl. The water potential decrease was realized either rapidly (osmotic shock) or slowly (20 min) and a difference in cell viability between these conditions was on...

journal_title：Applied microbiology and biotechnology

authors： Poirier I,Maréchal PA,Evrard C,Gervais P

更新日期：1998-12-01 00:00:00

abstract：:Due to its potential in preventing or slowing the occurrence of many diseases, resveratrol (3,5,4'-trihydroxystilbene) has attracted great research interest. The objective of this study was to identify microorganisms from selected plants that produce resveratrol and to optimize the conditions for resveratrol productio...

journal_title：Applied microbiology and biotechnology

authors： Shi J,Zeng Q,Liu Y,Pan Z

更新日期：2012-07-01 00:00:00

abstract：:Vinegar is a traditional fermented condiment, and the microbial diversity of DaQu makes the quality of vinegar products. Recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. In this study, the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar was analyz...

journal_title：Applied microbiology and biotechnology

authors： Li P,Li S,Cheng L,Luo L

更新日期：2014-07-01 00:00:00

abstract：:Biofilms are widely used in wastewater treatment for their particular enhancement of nitrogen removal and other significant advantages. In this study, the diversity and potential functions of nitrogen removal bacteria in suspended activated sludge (AS) and biofilm of a full-scale hybrid reactor were uncovered by metag...

journal_title：Applied microbiology and biotechnology

authors： Chao Y,Mao Y,Yu K,Zhang T

更新日期：2016-09-01 00:00:00

abstract：:Only two polyethylene glycol terephthalate (PET)-degrading enzymes have been reported, and their mechanism for the biochemical degradation of PET remains unclear. To identify a novel PET-degrading enzyme, a putative cutinase gene (cut190) was cloned from the thermophile Saccharomonospora viridis AHK190 and expressed i...

journal_title：Applied microbiology and biotechnology

authors： Kawai F,Oda M,Tamashiro T,Waku T,Tanaka N,Yamamoto M,Mizushima H,Miyakawa T,Tanokura M

更新日期：2014-12-01 00:00:00

abstract：:Mycobacterium tuberculosis is able to transition into a dormant state, causing a latent state of tuberculosis. Dormant mycobacteria acquire phenotypic resistance to all known antibacterial drugs; they are also able to maintain vitality in the host for decades and become active, causing the active form of the disease. ...

journal_title：Applied microbiology and biotechnology

authors： Shleeva MO,Savitsky AP,Nikitushkin VD,Solovyev ID,Kazachkina NI,Perevarov VV,Kaprelyants AS

更新日期：2019-12-01 00:00:00

abstract：:Dengue viruses are emerging mosquito-borne pathogens belonging to Flaviviridae family which are transmitted to humans via the bites of infected mosquitoes Aedes aegypti and Aedes albopictus. Because of the wide distribution of these mosquito vectors, more than 2.5 billion people are approximately at risk of dengue inf...

journal_title：Applied microbiology and biotechnology

authors： Fahimi H,Mohammadipour M,Haddad Kashani H,Parvini F,Sadeghizadeh M

更新日期：2018-04-01 00:00:00

abstract：:Reduction of azo dye methyl red coupled with aerobic respiration by growing cultures of Klebsiella oxytoca GS-4-08 was investigated. In liquid media containing dye and 0.6 % glucose in a mineral salts base, 100 mg l(-1) of the dye are completely removed in 3 h under shaking conditions. The dye cannot be aerobically de...

journal_title：Applied microbiology and biotechnology

authors： Yu L,Zhang XY,Xie T,Hu JM,Wang S,Li WW

更新日期：2015-03-01 00:00:00

abstract：:Clostridium tyrobutyricum is a promising organism for butyrate and n-butanol production, but cannot grow on sucrose. Three genes (scrA, scrB, and scrK) involved in the sucrose catabolic pathway, along with an aldehyde/alcohol dehydrogenase gene, were cloned from Clostridium acetobutylicum and introduced into C. tyrobu...

journal_title：Applied microbiology and biotechnology

authors： Zhang J,Yu L,Xu M,Yang ST,Yan Q,Lin M,Tang IC

更新日期：2017-05-01 00:00:00

abstract：:Homologous recombination-based recombineering is a widely used DNA cloning and modification technique; recombineering efficiency improvement would be helpful for high-throughput DNA manipulation. Escherichia coli primase DnaG variants, such as DnaG Q576A and DnaG K580A, increase the recombineering efficiency via impai...

journal_title：Applied microbiology and biotechnology

authors： Li J,Sun J,Gao X,Wu Z,Shang G

更新日期：2019-04-01 00:00:00

abstract：:Aminotransferases (ATs) are important biocatalysts for the synthesis of chiral amines because of their capability of introducing amino group into ketones or keto acids as well as their high enantioselectivity, high regioselectivity. Among all ATs, branched-chain amino acid aminotransferase (BCAT) can use branched-chai...

journal_title：Applied microbiology and biotechnology

authors： Zheng X,Cui Y,Li T,Li R,Guo L,Li D,Wu B

更新日期：2019-10-01 00:00:00

abstract：:Kimchi, a traditional Korean food made by the fermentation of vegetables, has become popular globally because of its organoleptic, beneficial, and nutritional properties. Spontaneous kimchi fermentation in unsterilized raw materials leads to the growth of various lactic acid bacteria (LAB), which results in variations...

journal_title：Applied microbiology and biotechnology

authors： Jung JY,Lee SH,Jeon CO

更新日期：2014-03-01 00:00:00

abstract：:The novel influenza A virus (IAV) defective interfering particle "OP7" inhibits IAV replication in a co-infection and was previously suggested as a promising antiviral agent. Here, we report a batch-mode cell culture-based production process for OP7. In the present study, a seed virus containing standard virus (STV) a...

journal_title：Applied microbiology and biotechnology

authors： Hein MD,Kollmus H,Marichal-Gallardo P,Püttker S,Benndorf D,Genzel Y,Schughart K,Kupke SY,Reichl U

更新日期：2021-01-01 00:00:00

abstract：:This study aimed to model the inactivation of Lactobacillus brevis DSM 6235 while retaining the viability of yeasts during washing brewer's yeast with phosphoric acid and chlorine dioxide. The independent variables in the acid washing were pH (1-3) and temperature (1-9 °C), whereas in the washing with chlorine dioxide...

journal_title：Applied microbiology and biotechnology

authors： Munford ARG,Chaves RD,Granato D,Sant'Ana AS

更新日期：2020-05-01 00:00:00

abstract：:The araA gene encoding an L-arabinose isomerase (L-AI) from the acido-thermophilic bacterium Acidothermus cellulolytics ATCC 43068 was cloned and overexpressed in Escherichia coli. The open reading frame of the L-AI consisted of 1,503 nucleotides encoding 501 amino acid residues. The recombinant L-AI was purified to h...

journal_title：Applied microbiology and biotechnology

authors： Cheng L,Mu W,Zhang T,Jiang B

更新日期：2010-04-01 00:00:00

abstract：:Phomopsis sp. XP-8 is an endophytic fungus that has the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application for the biosynthesis of PDG independent of plants. When cultivated in mung bean medium, PDG production was significantly improved and pinoresinol monoglucoside (PMG) and ...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Shi J,Gao Z,Yangwu R,Jiang H,Che J,Liu Y

更新日期：2015-06-01 00:00:00

abstract：:Ralstonia eutropha H850 was labelled chromosomally with a gfp marker gene encoding for the green fluorescent protein, and designated R. eutropha H850g13. Visual observation of green fluorescent cells under an epifluorescence microscope, and PCR amplification products, confirmed that the bacterium was labelled with gfp...

journal_title：Applied microbiology and biotechnology

authors： Abbey AM,Beaudette LA,Lee H,Trevors JT

更新日期：2003-12-01 00:00:00

abstract：:The marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental c...

journal_title：Applied microbiology and biotechnology

authors： Kautharapu KB,Rathmacher J,Jarboe LR

更新日期：2013-04-01 00:00:00

abstract：:India is the fifth largest primary energy consumer and fourth largest petroleum consumer after USA, China, and Japan. Despite the global economic crisis, India's economy is expected to grow at 6 to 8 %/year. There is an extreme dependence on petroleum products with considerable risks and environmental issues. Petroleu...

journal_title：Applied microbiology and biotechnology

authors： Hemaiswarya S,Raja R,Carvalho IS,Ravikumar R,Zambare V,Barh D

更新日期：2012-12-01 00:00:00

abstract：:The ability of taxonomically different yeasts to synthesize pyruvic acid (PA) from glucose was studied. The study showed that many yeasts are able to produce PA from glucose under the condition of growth limitation by thiamine. This ability was found in the yeast Blastobotrys adeninivorans for the first time. The prod...

journal_title：Applied microbiology and biotechnology

authors： Kamzolova SV,Morgunov IG

更新日期：2016-09-01 00:00:00

abstract：:We previously showed that opdA from Sphingomonas sp. PWE1 encodes a putative flavin monooxygenase capable of transforming octylphenol (OP) via type II ipso substitution. Here, we demonstrate that an opdA homolog is responsible for OP and related alkyl/alkoxyphenol degradation in the nonylphenol degrader Sphingomonas s...

journal_title：Applied microbiology and biotechnology

authors： Porter AW,Campbell BR,Kolvenbach BA,Corvini PF,Benndorf D,Rivera-Cancel G,Hay AG

更新日期：2012-04-01 00:00:00

abstract：:Orange peel waste (OPW), the primary byproduct of the juice extraction process, is annually generated in massive amounts (21 Mton), and its aqueous extraction in biorefining operations yields a liquid fraction, referred to as orange peel extract (OPE). Although OPE contains significant amounts of easily assimilable ca...

journal_title：Applied microbiology and biotechnology

authors： Carota E,Petruccioli M,D'Annibale A,Gallo AM,Crognale S

更新日期：2020-05-01 00:00:00

abstract：:Gene amplification using dihydrofolate reductase gene (dhfr) and methotrexate (MTX) is widely used for recombinant protein production in mammalian cells and is typically conducted in DHFR-deficient Chinese hamster ovary (CHO) cell lines. Generation of DHFR-deficient cells can be achieved by an expression vector incorp...

journal_title：Applied microbiology and biotechnology

authors： Kang SY,Kim YG,Lee HW,Lee EG

更新日期：2015-12-01 00:00:00