Photoinactivation of dormant Mycobacterium smegmatis due to its endogenous porphyrins.

abstract：:The demand of renewable energy sources, i.e. biomass, is steadily increasing worldwide to reduce the need of fossil energy sources. Biomass such as energy crops, woody species, forestry and agricultural residues are the most common renewable energy sources. Due to uneven demand for wood fuel, the material is mostly st...

journal_title：Applied microbiology and biotechnology

authors： Noll M,Jirjis R

更新日期：2012-08-01 00:00:00

abstract：:Uniform stable-isotope labeling of mammalian cells is achieved via a novel formulation of a serum-free cell culture medium that is based on stable-isotope-labeled autolysates and lipid extracts of various microbiological origin. Yeast autolysates allow complete replacement of individual amino acids and organic acids i...

journal_title：Applied microbiology and biotechnology

authors： Egorova-Zachernyuk TA,Bosman GJ,Degrip WJ

更新日期：2011-01-01 00:00:00

abstract：:We describe the discovery, isolation and characterization of a highly thermostable alditol oxidase from Acidothermus cellulolyticus 11B. This protein was identified by searching the genomes of known thermophiles for enzymes homologous to Streptomyces coelicolor A3(2) alditol oxidase (AldO). A gene (sharing 48% protein...

journal_title：Applied microbiology and biotechnology

authors： Winter RT,Heuts DP,Rijpkema EM,van Bloois E,Wijma HJ,Fraaije MW

更新日期：2012-07-01 00:00:00

abstract：:A maltotriose-producing α-amylase, AmyA, from a newly isolated bacterial strain Microbulbifer thermotolerans DAU221 was purified and characterized in the heterologous host, Escherichia coli, using the pCold I vector. The amyA gene encoded a 761-residue protein composed of a 33 amino acid secretion signal peptide. The ...

journal_title：Applied microbiology and biotechnology

authors： Lee YS,Park DJ,Choi YL

更新日期：2015-05-01 00:00:00

abstract：:The temporal and spatial distribution of the genus Bacillus and Clostridium histolyticum group in swine manure composting was determined by fluorescent in situ hybridization using fluorescently labeled 16S rRNA-targeted oligonucleotide probes LGC353b and Chis150, respectively. The temporal distribution of total bacter...

journal_title：Applied microbiology and biotechnology

authors： Yi J,Zheng R,Li F,Chao Z,Deng CY,Wu J

更新日期：2012-03-01 00:00:00

abstract：:Two cephalosporin genes from Acremonium chrysogenum, pcbAB and pcbC encode the ACV (alpha-aminoadipyl-cysteinyl-valine) synthetase and isopenicillin N-synthetase, respectively. The two adjacent genes are orientated in opposite directions on the chromosomal DNA, separated by a 1.2-kb non-translated sequence, carrying t...

journal_title：Applied microbiology and biotechnology

authors： Menne S,Walz M,Kück U

更新日期：1994-10-01 00:00:00

abstract：:Dengue virus (DENV) and Japanese encephalitis virus (JEV) are closely related mosquito-borne flaviviruses. Together, they caused most arthropod-borne diseases in the world. Previously, we had demonstrated that both live attenuated and inactivated JE vaccines elicited cross-protection against DENV infection, and a DNA ...

journal_title：Applied microbiology and biotechnology

authors： Gao N,Li J,Sheng Z,Chen H,Fan D,Wang P,An J

更新日期：2019-06-01 00:00:00

abstract：:This study focused on the use the thermophilic anaerobic granulae in the start-up of 70 degrees C acetate-fed upflow anaerobic sludge-blanket (UASB) reactors and the kinetics of granulae grown at 70 degrees C. In the UASB reactors, chemical oxygen demand removal commenced within 48 h of the start-up. The maximum reduc...

journal_title：Applied microbiology and biotechnology

authors： Lepistö R,Rintala JA

更新日期：1995-11-01 00:00:00

abstract：:Due to efficient photosynthetic capability, robust growth, and clear genetic background, cyanobacteria are recently used for production of different biofuel and biochemical molecules by genetic engineering and showed great potentials as the next-generation microbial cell factory. For improving the production of bio-pr...

journal_title：Applied microbiology and biotechnology

authors： Tan X,Liang F,Cai K,Lu X

更新日期：2013-07-01 00:00:00

abstract：:In recent years, the research on aerobic granulation has been intensive. So far, almost all aerobic granules can form only in sequencing batch reactors (SBR), while the reason is not yet understood. This paper attempts to review the factors involved in aerobic granulation in SBR, including substrate composition, organ...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Wang ZW,Qin L,Liu YQ,Tay JH

更新日期：2005-04-01 00:00:00

abstract：:The hydrolysis of cellulose into fermentable sugars is a costly and rate-limiting step in the production of biofuels from renewable feedstocks. Developing new cellulase systems capable of increased cellulose hydrolysis rates would reduce biofuel production costs. With this in mind, we screened 55 fungal endoglucanases...

journal_title：Applied microbiology and biotechnology

authors： Tambor JH,Ren H,Ushinsky S,Zheng Y,Riemens A,St-Francois C,Tsang A,Powlowski J,Storms R

更新日期：2012-01-01 00:00:00

abstract：:Previously, we reported that the expression of Bombyx mori 30Kc6 gene in Chinese hamster ovary (CHO) cells increases recombinant protein production by both inhibiting apoptosis and enhancing specific productivity. In this study, in order to gain a thorough understanding of the roles of 30Kc6 gene in antibody productio...

journal_title：Applied microbiology and biotechnology

authors： Wang Z,Ma X,Fan L,Rhee WJ,Park TH,Zhao L,Tan WS

更新日期：2012-06-01 00:00:00

abstract：:The genetic improvement of winemaking yeasts is a virtually infinite process, as the design of new strains must always cope with varied and ever-evolving production contexts. Good wine yeasts must feature both good primary traits, which are related to the overall fermentative fitness of the strain, and secondary trait...

journal_title：Applied microbiology and biotechnology

authors： Bonciani T,De Vero L,Mezzetti F,Fay JC,Giudici P

更新日期：2018-03-01 00:00:00

abstract：:Acrylic polymers (AP) are a diverse group of materials with broad applications, frequent use, and increasing demand. Some of the most used AP are polyacrylamide, polyacrylic acid, polymethyl methacrylates, and polyacrylonitrile. Although no information for the production of all AP types is published, data for the most...

journal_title：Applied microbiology and biotechnology

authors： Gaytán I,Burelo M,Loza-Tavera H

更新日期：2021-01-11 00:00:00

abstract：:Recently, a new gene encoding beta-glucuronidase from Streptococcus equi subsp. zooepidemicus (SEZ) was identified and expressed in Escherichia coli. In this paper, the characterization of the enzyme is described. Specific enzyme activity was 120,000 U/mg purified protein at 37 degrees C and pH = 7.0. The temperature ...

journal_title：Applied microbiology and biotechnology

authors： Krahulec J,Krahulcová J

更新日期：2007-04-01 00:00:00

abstract：:Extracellular polymeric substance (EPS) is proposed to facilitate calcium ion supersaturation through its nucleation effect during the microbially induced calcium carbonate precipitation (MICP) process. However, the supersaturation effect of Ca2+ via EPS in MICP has not been clearly demonstrated. Enhanced exopolysacch...

journal_title：Applied microbiology and biotechnology

authors： Kim HJ,Shin B,Lee YS,Park W

更新日期：2017-08-01 00:00:00

abstract：:This study found that the ratio of Thiothrix eikelboomii to total bacterial concentrations (TH/TB) (%) was a better indicator of bulking incidents affecting effluent quality compared to absolute T. eikelboomii abundance alone. This was determined using a genus-specific Thiothrix quantitative PCR primer and probe set, ...

journal_title：Applied microbiology and biotechnology

authors： Asvapathanagul P,Olson BH,Gedalanga PB,Hashemi A,Huang Z,La J

更新日期：2015-05-01 00:00:00

abstract：:Soluble expression of recombinant therapeutic proteins in Escherichia coli (E. coli) has been a challenging task in biopharmaceutical development. In this study, a novel self-cleavable tag Zbasic-intein has been constructed for the soluble expression and purification of a recombinant cytokine, human interleukin-15 (IL...

journal_title：Applied microbiology and biotechnology

authors： Shi S,Chen H,Jiang H,Xie Y,Zhang L,Li N,Zhu C,Chen J,Luo H,Wang J,Feng L,Lu H,Zhu J

更新日期：2017-02-01 00:00:00

abstract：:Vinegar is a traditional fermented condiment, and the microbial diversity of DaQu makes the quality of vinegar products. Recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. In this study, the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar was analyz...

journal_title：Applied microbiology and biotechnology

authors： Li P,Li S,Cheng L,Luo L

更新日期：2014-07-01 00:00:00

abstract：:Discovery of keratin-degrading enzymes from fungi and bacteria has primarily focused on finding one protease with efficient keratinase activity. Recently, an investigation was conducted of all keratinases secreted from a fungus known to grow on keratinaceous materials, such as feather, horn, and hooves. The study demo...

journal_title：Applied microbiology and biotechnology

authors： Lange L,Huang Y,Busk PK

更新日期：2016-03-01 00:00:00

abstract：:This study has investigated the acclimation of ammonia-oxidizing communities (AOC) to low dissolved oxygen (DO) concentrations. Under controlled laboratory conditions, two sequencing batch reactors seeded with activated sludge from the same source were operated at high DO (near saturation) and low DO (0.1 mg O₂/L) con...

journal_title：Applied microbiology and biotechnology

authors： Arnaldos M,Kunkel SA,Stark BC,Pagilla KR

更新日期：2013-12-01 00:00:00

abstract：:Grape juice contains about equal amounts of glucose and fructose, but wine strains of Saccharomyces cerevisiae ferment glucose slightly faster than fructose, leading to fructose concentrations that exceed glucose concentrations in the fermenting must. A high fructose/glucose ratio may contribute to sluggish and stuck ...

journal_title：Applied microbiology and biotechnology

authors： Berthels NJ,Cordero Otero RR,Bauer FF,Pretorius IS,Thevelein JM

更新日期：2008-01-01 00:00:00

abstract：:Expressing proteins with fusion partners improves yield and simplifies the purification process. We developed a novel fusion partner to improve the secretion of heterologous proteins that are otherwise poorly excreted in yeast. The VOA1 (YGR106C) gene of Saccharomyces cerevisiae encodes a subunit of vacuolar ATPase. W...

journal_title：Applied microbiology and biotechnology

authors： Bae JH,Sung BH,Seo JW,Kim CH,Sohn JH

更新日期：2016-12-01 00:00:00

abstract：:The original version of this article was revised: After publication of this article, the publisher was notified that Michael Klocke has been listed as an author without his consent. ...

journal_title：Applied microbiology and biotechnology

authors： Carabeo-Pérez A,Guerra-Rivera G,Ramos-Leal M,Jiménez-Hernández J

更新日期：2019-12-01 00:00:00

abstract：:A flocculent Saccharomyces cerevisiae strain with the ability to express both the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus was constructed. This recombinant strain is not only able to grow on lactose, but it can also ferment this substrate. To our kn...

journal_title：Applied microbiology and biotechnology

authors： Domingues L,Teixeira JA,Lima N

更新日期：1999-05-01 00:00:00

abstract：:Ginsenosides are believed to be the principal components behind the pharmacological actions of ginseng, and their bioactive properties are closely related to the type, position, and number of sugar moieties attached to the aglycone; thus, modification of the sugar chains may markedly change their biological activities...

journal_title：Applied microbiology and biotechnology

authors： Tan JS,Yeo CR,Popovich DG

更新日期：2017-07-01 00:00:00

abstract：:To investigate the effect of fungal elicitors on biosynthesis of natamycin in the cultures of Streptomyces natalensis HW-2, the biomass and filtrate of the broth from Aspergillus niger AS 3.6472, Penicillium chrysogenum AS 3.5163, A. oryzae AS 3.2068, and Saccharomyces cerevisiae AS 2.2081 were used to induce natamyci...

journal_title：Applied microbiology and biotechnology

authors： Wang D,Yuan J,Gu S,Shi Q

更新日期：2013-06-01 00:00:00

abstract：:Surfaces of massive chalcopyrite (CuFeS2) electrodes were modified by applying variable oxidation potential pulses under growth media in order to induce the formation of different secondary phases (e.g., copper-rich polysulfides, S n(2-); elemental sulfur, S(0); and covellite, CuS). The evolution of reactivity (oxidat...

journal_title：Applied microbiology and biotechnology

authors： Lara RH,García-Meza JV,González I,Cruz R

更新日期：2013-03-01 00:00:00

abstract：:Diosgenin transformation was studied in Streptomyces virginiae IBL-14, a soil-dwelling bacterium with diosgenin-degrading capacity. All of the derivatives isolated were identified as 4-ene-3-keto steroids. We cloned ChoL, a fragment of a cholesterol oxidase from S. virginiae IBL-14, and used gene-disruption techniques...

journal_title：Applied microbiology and biotechnology

authors： Li B,Wang W,Wang FQ,Wei DZ

更新日期：2010-02-01 00:00:00

abstract：:Expression of recombinant proteins in Escherichia coli often requires use of inducible promoters to shorten the lag phase and improve protein productivity and final protein titer. Synthetic molecules that cannot be metabolized by E. coli, such as isopropyl thiogalactopyranoside (IPTG), have been frequently used to tri...

journal_title：Applied microbiology and biotechnology

authors： Anilionyte O,Liang H,Ma X,Yang L,Zhou K

更新日期：2018-08-01 00:00:00