A novel fusion partner for enhanced secretion of recombinant proteins in Saccharomyces cerevisiae.

abstract：:Non-covalent interaction of alcohol dehydrogenase with polysaccharides was studied using three neutral and three anionic polysaccharides. The process of interaction of alcohol dehydrogenase with gum Arabic was optimized with respect to the ratio of enzyme to gum Arabic, pH, and molarity of buffer. Alcohol dehydrogenas...

journal_title：Applied microbiology and biotechnology

authors： Jadhav SB,Bankar SB,Granström T,Ojamo H,Singhal RS,Survase SA

更新日期：2014-01-01 00:00:00

abstract：:The Vitreoscilla hemoglobin (VHb) gene (vgb) was integrated into the chromosome of Bacillus thuringiensis BMB171 using integrative vector pEG491. The production of VHb was confirmed by CO-difference spectra analysis. Fermentation experiments results showed that with the production of VHb, the critical oxygen concentra...

journal_title：Applied microbiology and biotechnology

authors： Liang F,Shouwen C,Ming S,Ziniu Y

更新日期：2007-02-01 00:00:00

abstract：:Nitrate, injected into oil fields, can oxidize sulfide formed by sulfate-reducing bacteria (SRB) through the action of nitrate-reducing sulfide-oxidizing bacteria (NR-SOB). When reservoir rock contains siderite (FeCO(3)), the sulfide formed is immobilized as iron sulfide minerals, e.g. mackinawite (FeS). The aim of ou...

journal_title：Applied microbiology and biotechnology

authors： Lin S,Krause F,Voordouw G

更新日期：2009-05-01 00:00:00

abstract：:Deoxynucleoside-5'-monophosphates (5'-dNMPs) are the basic components of DNA and are widely used in medicine and as chemical and biochemical reagents. A large amount of effort has been expended to obtain 5'-dNMPs of high quality and at a low cost. However, these procedures are inefficient and inconvenient. In this stu...

journal_title：Applied microbiology and biotechnology

authors： Zou Z,Ding Q,Ou L,Yan B

更新日期：2013-11-01 00:00:00

abstract：:Bacterial glucans have aroused increasing interest in commercial applications in the food and pharmaceutical sectors. A number of bacterial glucans have been reported over recent decades, and their structure, production, and functional properties have been extensively studied. In this paper, we review recent researche...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Zhang J

更新日期：2016-11-01 00:00:00

abstract：:Laccases are blue multicopper oxidases with potential applications in environmental and industrial biotechnology. In this study, a new bacterial laccase gene of 1.32 kb was obtained from a marine microbial metagenome of the South China Sea by using a sequence screening strategy. The protein (named as Lac15) of 439 ami...

journal_title：Applied microbiology and biotechnology

authors： Fang Z,Li T,Wang Q,Zhang X,Peng H,Fang W,Hong Y,Ge H,Xiao Y

更新日期：2011-02-01 00:00:00

abstract：:A new medium supplement mixture, PPRF92, has been developed to enable the serial subculture of human diploid fibroblasts (MRC-5 cells) on microcarriers. Furthermore, the PPRF92 supplements enable cell growth at serum levels as low as 1%. Through an optimization programme, the PPRF92 supplements have evolved into a sim...

journal_title：Applied microbiology and biotechnology

authors： Forestell SP,Kalogerakis N,Behie LA

更新日期：1992-11-01 00:00:00

abstract：:The hemiascomycete Ashbya gossypii develops a mycelium. Nutritional stress leads to its differentiation into sporangia. These generate spores. In parallel, the yellow pigment riboflavin is produced. Intracellularly accumulated riboflavin, made visible as a bright green fluorescence, was observed in only 60% of the hyp...

journal_title：Applied microbiology and biotechnology

authors： Nieland S,Stahmann KP

更新日期：2013-12-01 00:00:00

abstract：:Aspergillus oryzae is a fungus used extensively in the fermentation industry. We constructed cDNA microarrays comprising 2,070 highly expressed cDNAs selected from the approximately 6,000 non-redundant expressed sequence tags (ESTs) in the A. oryzae EST database (http://www.aist.go.jp/RIODB/ffdb/index.html). Using the...

journal_title：Applied microbiology and biotechnology

authors： Maeda H,Sano M,Maruyama Y,Tanno T,Akao T,Totsuka Y,Endo M,Sakurada R,Yamagata Y,Machida M,Akita O,Hasegawa F,Abe K,Gomi K,Nakajima T,Iguchi Y

更新日期：2004-07-01 00:00:00

abstract：:The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 degrees C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability de...

journal_title：Applied microbiology and biotechnology

authors： Beney L,Marechal PA,Gervais P

更新日期：2001-08-01 00:00:00

abstract：:Two novel cyclic hexapeptides designated actinosynneptides A (1) and B (2), together with three tryptophan containing diketopiperazines, namely cyclo(L-Trp-L-Trp) (3), cyclo(L-Trp-N-MeL-Trp) (4), and cyclo(N-MeL-Trp-N-MeL-Trp) (5), were isolated from the culture of the genetically engineered strain HGF052::asm18 deriv...

journal_title：Applied microbiology and biotechnology

authors： Lu C,Xie F,Shan C,Shen Y

更新日期：2017-03-01 00:00:00

abstract：:The growing demand for sustainable animal production is compelling researchers to explore the potential approaches to reduce emissions of greenhouse gases from livestock that are mainly produced by enteric fermentation. Some potential solutions, for instance, the use of chemical inhibitors to reduce methanogenesis, ar...

journal_title：Applied microbiology and biotechnology

authors： Kumar S,Choudhury PK,Carro MD,Griffith GW,Dagar SS,Puniya M,Calabro S,Ravella SR,Dhewa T,Upadhyay RC,Sirohi SK,Kundu SS,Wanapat M,Puniya AK

更新日期：2014-01-01 00:00:00

abstract：:Multidrug-resistant Acinetobacter baumannii is a well-documented pathogen associated with hospital-acquired infections. In addition to multidrug resistance, A. baumannii can also become resistant to colistin, the antibiotic treatment of last resort, by the loss of the lipopolysaccharide from its outer membrane. Here, ...

journal_title：Applied microbiology and biotechnology

authors： Tseng CC,Tsai YH,Hu A,Liou JW,Chang KC,Chang HH

更新日期：2016-10-01 00:00:00

abstract：:Streptomyces viridochromogenes Tü494 produces the antibiotic phosphinothricyl-alanyl-alanine (Ptt). Ptt-non-producing mutants were isolated following N-methyl-N'-nitro-N-nitrosoguanidine (NTG) or UV light treatment of spore suspensions. In co-synthesis and bioconversion experiments the mutational blocks in the biosynt...

journal_title：Applied microbiology and biotechnology

authors： Alijah R,Dorendorf J,Talay S,Pühler A,Wohlleben W

更新日期：1991-03-01 00:00:00

abstract：:Zygosaccharomyces rouxii plays important roles in the brewing process of fermented foods such as soy sauce, where salt stress is a frequently encountered condition. In this study, effect of heat preadaptation on salt tolerance of Z. rouxii and the protective mechanisms underlying heat preadaptation were investigated b...

journal_title：Applied microbiology and biotechnology

authors： Wang D,Zhang M,Huang J,Zhou R,Jin Y,Zhao D,Zheng J,Wu C

更新日期：2021-01-01 00:00:00

abstract：:Increasing evidence has shown that fecal microbiota transplantation (FMT) could be a promising treatment option for Crohn's disease (CD). However, the frequency of FMT for CD treatment remains unclear. This study aimed to evaluate the optimal timing for administering the second course of FMT to maintain the long-term ...

journal_title：Applied microbiology and biotechnology

authors： Li P,Zhang T,Xiao Y,Tian L,Cui B,Ji G,Liu YY,Zhang F

更新日期：2019-01-01 00:00:00

abstract：:Knowledge of tolerance of bacteria to toxic stress is important, especially for processes targeted at high final titers of product. Information on environmental limits and stress responses may help during selection of strains or design and control of processes. The influence of the main product and its co-products on ...

journal_title：Applied microbiology and biotechnology

authors： Szymanowska-Powałowska D,Kubiak P

更新日期：2015-04-01 00:00:00

abstract：:A new method is proposed for the evaluation of the activity of sourdough strains, based on gas pressure measurements in closed air-tight reactors. Gas pressure and pH were monitored on-line during the cultivation of commercial yeasts and heterofermentative lactic acid bacteria on a semi-synthetic medium with glucose a...

journal_title：Applied microbiology and biotechnology

authors： Wick M,Vanhoutte JJ,Adhemard A,Turini G,Lebeault JM

更新日期：2001-04-01 00:00:00

abstract：:Intervention strategies for obesity are global issues that require immediate attention. The objective of this study was to assess the possibility that Clostridium butyricum and its potential components could reduce lipogenesis. Co-culture experiments of Caco-2 cells and 1 × 10(6), 1 × 10(7), and 1 × 10(8) CFU/ml of C....

journal_title：Applied microbiology and biotechnology

authors： Zhao X,Guo Y,Liu H,Gao J,Nie W

更新日期：2014-09-01 00:00:00

abstract：:Smokeless tobacco (ST) products are used worldwide and are a major public health concern. In addition to harmful chemicals found in these products, microbes found in ST products are believed to be responsible for generating harmful tobacco-specific nitrosamines (TSNAs), the most abundant carcinogens in ST. These micro...

journal_title：Applied microbiology and biotechnology

authors： Tyx RE,Rivera AJ,Keong LM,Stanfill SB

更新日期：2020-01-01 00:00:00

abstract：:The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes...

journal_title：Applied microbiology and biotechnology

authors： Jin FJ,Han P,Zhuang M,Zhang ZM,Jin L,Koyama Y

更新日期：2018-01-01 00:00:00

abstract：:An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regi...

journal_title：Applied microbiology and biotechnology

authors： Yu IS,Yeom SJ,Kim HJ,Lee JK,Kim YH,Oh DK

更新日期：2008-02-01 00:00:00

abstract：:Yarrowia lipolytica is an industrial yeast that has been used in the sustainable production of fatty acid-derived and lipid compounds due to its high growth capacity, genetic tractability, and oleaginous properties. This investigation examines the possibility of utilizing urea or urine as an alternative to ammonium su...

journal_title：Applied microbiology and biotechnology

authors： Brabender M,Hussain MS,Rodriguez G,Blenner MA

更新日期：2018-03-01 00:00:00

abstract：:Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level alm...

journal_title：Applied microbiology and biotechnology

authors： Faulds CB,Williamson G

更新日期：1995-11-01 00:00:00

abstract：:Crude peroxidase preparations from the lignocellulose-degrading actinomycete, Streptomyces viridosporus T7A, were shown to decolorize several azo dye isomers and showed a correlation of dye structure to degradability similar to that shown by fungal Mn-peroxidase, an enzyme not previously described in actinomycetes. Ad...

journal_title：Applied microbiology and biotechnology

authors： Burke NS,Crawford DL

更新日期：1998-05-01 00:00:00

abstract：:Prenyltransferase NovQ is a vital class involved in the biosynthesis of secondary metabolites such as clorobiocin and novobiocin. To investigate the relationship between structure and catalytic properties of NovQ, here, we have analyzed the substrate-binding site, namely PT barrel, and revealed that menadione hydroqui...

journal_title：Applied microbiology and biotechnology

authors： Ni W,Zheng Z,Liu H,Wang P,Wang H,Sun X,Yang Q,Fang Z,Tang H,Zhao G

更新日期：2020-05-01 00:00:00

abstract：:Bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. The well-defined and stable mutants do not form functional endospores due to the deletion of yqfD, an essential sporulation gene; they are affected in DNA repair,...

journal_title：Applied microbiology and biotechnology

authors： Wemhoff S,Meinhardt F

更新日期：2013-09-01 00:00:00

abstract：:Poly(ɛ-L-lysine) (ɛ-PL) is an unusual biopolymer composed of L-lysine connected between α-carboxyl and ɛ-amino groups. It has been used as a preservative in food and cosmetics industries, drug carrier in medicines, and gene carrier in gene therapy. Modern biotechnology has significantly improved the synthetic efficien...

journal_title：Applied microbiology and biotechnology

authors： Xu Z,Xu Z,Feng X,Xu D,Liang J,Xu H

更新日期：2016-08-01 00:00:00

abstract：:Agar is a major polysaccharide of red algal cells and is mainly decomposed into neoagarobiose by the co-operative effort of β-agarases. Neoagarobiose is hydrolyzed into monomers, D-galactose and 3,6-anhydro-L-galactose, via a microbial oxidative process. Therefore, the enzyme, 1,3-α-3,6-anhydro-L-galactosidase (α-neoa...

journal_title：Applied microbiology and biotechnology

authors： Asghar S,Lee CR,Park JS,Chi WJ,Kang DK,Hong SK

更新日期：2018-10-01 00:00:00

abstract：:In an effort to produce ricinoleic acid (RA), an important natural raw material with great values as a petrochemical replacement, in Schizosaccharomyces pombe, we introduced Claviceps purpurea oleate Δ12-hydroxylase gene (CpFAH12) to S. pombe, putting it under the control of an inducible nmt1 promoter. However, RA was...

journal_title：Applied microbiology and biotechnology

authors： Yazawa H,Holic R,Kumagai H,Uemura H

更新日期：2013-09-01 00:00:00