Enhanced stability of alcohol dehydrogenase by non-covalent interaction with polysaccharides.

abstract：:Bacteria, fungi, viruses, and nematodes are the major causal agents of plant diseases. These phytopathogens are responsible for about 10-40% losses in productivity and quality of food crops and horticultural produce. Although eradication of pathogens is not possible, control of plant diseases has been an area of conti...

journal_title：Applied microbiology and biotechnology

authors： Rajwade JM,Chikte RG,Paknikar KM

更新日期：2020-02-01 00:00:00

abstract：:4AaCter is the polypeptide from the C-terminal extension of mosquitocidal Cry4Aa toxin, and facilitates formation of protein inclusion in Escherichia coli. It has been demonstrated that the use of 4AaCter as a peptide tag results in the efficient production of heterologous protein in E. coli. It has also been demonstr...

journal_title：Applied microbiology and biotechnology

authors： Hayakawa T,Shimizu Y,Ishida T,Sakai H

更新日期：2011-06-01 00:00:00

abstract：:This research investigated the effect of microwave irradiation (MWI) on cell disintegration in municipal secondary sludge (MSS). A representative MSS Gram-positive bacterium (Bacillus subtilis) and Gram-negative bacteria (Acinetobacter calcoaceticus and Pseudomonas aeruginosa) were pure cultured separately and treated...

journal_title：Applied microbiology and biotechnology

authors： Zhou BW,Shin SG,Hwang K,Ahn JH,Hwang S

更新日期：2010-06-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae has been modified successfully for production of numerous metabolites and therapeutic proteins through metabolic engineering, but has not been utilized to date for the production of lipid-derived compounds. We developed a lipid metabolic engineering strategy in S. cerevisiae based up...

journal_title：Applied microbiology and biotechnology

authors： Dyer JM,Chapital DC,Kuan JW,Mullen RT,Pepperman AB

更新日期：2002-07-01 00:00:00

abstract：:Mine water is an example of an extreme environment that contains a large number of diverse and specific bacteria. It is imperative to gain an understanding of these bacterial communities in order to develop effective strategies for the bioremediation of polluted aquatic systems. In this study, the high-throughput sequ...

journal_title：Applied microbiology and biotechnology

authors： Keshri J,Mankazana BB,Momba MN

更新日期：2015-04-01 00:00:00

abstract：:Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system. However, it is unknown whether sialylated lactuloses have modulatory effects on the intestinal microbiota. ...

journal_title：Applied microbiology and biotechnology

authors： Song M,Zeng J,Jia T,Gao H,Zhang R,Jiang J,Li G,Su T

更新日期：2019-11-01 00:00:00

abstract：:Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples. EstM-N1 and EstM-N2 were 395 and 407 amino acids in length, respectively, and showed the highest similarity to class C...

journal_title：Applied microbiology and biotechnology

authors： Yu EY,Kwon MA,Lee M,Oh JY,Choi JE,Lee JY,Song BK,Hahm DH,Song JK

更新日期：2011-04-01 00:00:00

abstract：:We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was ...

journal_title：Applied microbiology and biotechnology

authors： Tanimura K,Takashima S,Matsumoto T,Tanaka T,Kondo A

更新日期：2016-07-01 00:00:00

abstract：:Dengue virus (DENV) and Japanese encephalitis virus (JEV) are closely related mosquito-borne flaviviruses. Together, they caused most arthropod-borne diseases in the world. Previously, we had demonstrated that both live attenuated and inactivated JE vaccines elicited cross-protection against DENV infection, and a DNA ...

journal_title：Applied microbiology and biotechnology

authors： Gao N,Li J,Sheng Z,Chen H,Fan D,Wang P,An J

更新日期：2019-06-01 00:00:00

abstract：:Fungal interspecific interactions enhance biosynthesis of phenylpropanoid metabolites (PM), and production of nitric oxide (NO) is known to be involved in this process. However, it remains unknown which signaling pathway(s) or regulator(s) mediate fungal PM biosynthesis. In this study, we cocultured two white-rot fung...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Xi Q,Xu Q,He M,Ding J,Dai Y,Keller NP,Zheng W

更新日期：2015-05-01 00:00:00

abstract：:Two compost piles were prepared, using two ventilation systems: forced ventilation and ventilation through mechanical turning. The material to compost was a mixture of orange waste, olive pomace, and grass clippings (2:1:1 v/v). During the composting period (375 days), samples were periodically taken from both piles, ...

journal_title：Applied microbiology and biotechnology

authors： Coelho L,Reis M,Dionísio L

更新日期：2013-05-01 00:00:00

abstract：:In insects, glutathione S-transferases (GSTs) are enzymes involved in detoxification of insecticides. However, few data are available for the codling moth, Cydia pomonella (L.). In this study, we cloned a delta class GST gene CpGSTd1 from C. pomonella. Real-time quantitative PCR shows that CpGSTd1 was up-regulated wit...

journal_title：Applied microbiology and biotechnology

authors： Liu J,Yang X,Zhang Y

更新日期：2014-11-01 00:00:00

abstract：:Based on our previous study evaluating the in vivo cure efficacy of chitosan on bovine mastitis, a more water-soluble chitosan-oligosaccharide (OCHT) with a high degree of deacetylation and low molecular weight was prepared to obtain high antibiotic efficacy. The growth of Staphylococcus aureus isolated from bovine ma...

journal_title：Applied microbiology and biotechnology

authors： Moon JS,Kim HK,Koo HC,Joo YS,Nam HM,Park YH,Kang MI

更新日期：2007-07-01 00:00:00

abstract：:The occurrence of killer toxins amongst yeast in Brazilian Riesling Italico grape must was investigated by using the sensitive strain EMBRAPA-26B as a reference strain at 18 degrees C and 28 degrees C. From a total of 85 previously isolated yeasts, 21 strains showed ability to kill the sensitive strain on unbuffered g...

journal_title：Applied microbiology and biotechnology

authors： da Silva GA

更新日期：1996-09-01 00:00:00

abstract：:Two lab-scale plug flow activated sludge reactors were run in parallel for 4 months at 30 and 55 degrees C. Research focussed on: (1) COD (chemical oxygen demand) removal, (2) effluent turbidity at both temperatures, (3) the origin of effluent colloidal material and (4) the possible role of protozoa on turbidity level...

journal_title：Applied microbiology and biotechnology

authors： Vogelaar JC,van Lier JB,Klapwijk B,de Vries MC,Lettinga G

更新日期：2002-06-01 00:00:00

abstract：:Furfural is one of the major inhibitors generated during sugar production from cellulosic materials and, as an aldehyde, inhibits various cellular activities of microorganisms used, leading to prolonged lag time during ethanologenic fermentation. Since Saccharomyces cerevisiae strains tolerant to furfural are of great...

journal_title：Applied microbiology and biotechnology

authors： Kim HS,Kim NR,Kim W,Choi W

更新日期：2012-07-01 00:00:00

abstract：:Recombinant DNA technology was used to insert a fetal liver genomic library ApaI fragment encoding for human erythropoietin (Epo) into Bowes melanoma cells. The cells expressed the erythropoietin gene, and Epo was secreted into the culture medium together with the normally-secreted tissue plasminogen activator. Attemp...

journal_title：Applied microbiology and biotechnology

authors： Keay L,Walsh JM,Buchholz RM,Terando JM

更新日期：1990-11-01 00:00:00

abstract：:Sphingobium sp. strain TCM1 can significantly degrade chlorinated organophosphorus flame retardants, such as tris(2-chloroethyl) phosphate. The PhoK of strain TCM1 (Sb-PhoK) is the main alkaline phosphatase (APase) that catalyzes the last step in the degradation pathway. Here, we purified and characterized Sb-PhoK pro...

journal_title：Applied microbiology and biotechnology

authors： Takahashi S,Morooka Y,Kumakura T,Abe K,Kera Y

更新日期：2020-02-01 00:00:00

abstract：:Protein titers, a key bioprocessing metric, depend both on the synthesis of protein and the degradation of protein. Secreted recombinant protein production in Saccharomyces cerevisiae is an attractive platform as minimal media can be used for cultivation, thus reducing fermentation costs and simplifying downstream pur...

journal_title：Applied microbiology and biotechnology

authors： Tyo KE,Liu Z,Magnusson Y,Petranovic D,Nielsen J

更新日期：2014-08-01 00:00:00

abstract：:There is emerging interest in the anaerobic degradation of crude oil. However, there is limited knowledge about the geochemical effects and microbiological activities for it. A mixture of anaerobic sludge and the production water from an oil well was used as an inoculum to construct four consortia, which were incubate...

journal_title：Applied microbiology and biotechnology

authors： Xiong S,Li X,Chen J,Zhao L,Zhang H,Zhang X

更新日期：2015-02-01 00:00:00

abstract：:Enzymes are natural catalysts highly specific to the substrate type and operate under mild conditions of temperature, pressure, and pH with high conversion rates, which makes them more efficient than conventional chemical catalysts. The enzymes can be obtained from various sources, animal, vegetable, and microbiologic...

journal_title：Applied microbiology and biotechnology

authors： Filho DG,Silva AG,Guidini CZ

更新日期：2019-09-01 00:00:00

abstract：:Exposure to bioaerosols can adversely influence human health through respiratory tract, eye, and skin irritation. Bioaerosol composition is unique on the International Space Station (ISS), where the size distribution of particles in the air differs from those on Earth. This is due to the lack of gravitational settling...

journal_title：Applied microbiology and biotechnology

authors： Haines SR,Bope A,Horack JM,Meyer ME,Dannemiller KC

更新日期：2019-09-01 00:00:00

abstract：:Due to its potential in preventing or slowing the occurrence of many diseases, resveratrol (3,5,4'-trihydroxystilbene) has attracted great research interest. The objective of this study was to identify microorganisms from selected plants that produce resveratrol and to optimize the conditions for resveratrol productio...

journal_title：Applied microbiology and biotechnology

authors： Shi J,Zeng Q,Liu Y,Pan Z

更新日期：2012-07-01 00:00:00

abstract：:To reveal the succession procedure of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) community structure in sequencing biofilm batch reactor (SBBR), the molecular biological techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and real-time PCR were applied. DGGE showed that th...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Zhang C,Rong H

更新日期：2014-05-01 00:00:00

abstract：:Biogranulation is a promising biotechnology developed for wastewater treatment. Biogranules exhibit a matrix microbial structure, and intensive research has shown that extracellular polymeric substances (EPS) are a major component of the biogranule matrix material in both anaerobic and aerobic granules. This paper aim...

journal_title：Applied microbiology and biotechnology

authors： Liu YQ,Liu Y,Tay JH

更新日期：2004-08-01 00:00:00

abstract：:A Gram-positive, red-pigment-producing bacterial strain, designated JS520 was isolated from the pristine sediment from the cave on mountain Miroc in Serbia. Strain was confirmed to belong to Streptomyces genus based on phenotypic and genetic analysis. Streptomyces sp. JS520 has the ability to produce exceptionally hig...

journal_title：Applied microbiology and biotechnology

authors： Stankovic N,Radulovic V,Petkovic M,Vuckovic I,Jadranin M,Vasiljevic B,Nikodinovic-Runic J

更新日期：2012-12-01 00:00:00

abstract：:In this study, the kinetic behaviors between acetone and composite bead were investigated. The microbial growth rate decreased with increasing average inlet concentration and increased with increasing operation temperature at average inlet concentration ranging from 50 to 300 ppm and operation temperature ranging from...

journal_title：Applied microbiology and biotechnology

authors： Chan WC,Chang LY

更新日期：2006-08-01 00:00:00

abstract：:β-Glucosidases designated MoCel3A and MoCel3B were successfully overexpressed in Magnaporthe oryzae. MoCel3A and MoCel3B showed optimal activity at 50 °C and pH 5.0-5.5. MoCel3A exhibited higher activity on higher degree of polymerization (DP) oligosaccharides and on β-1,3-linked oligosaccharides than on β-1,4-linked ...

journal_title：Applied microbiology and biotechnology

authors： Takahashi M,Konishi T,Takeda T

更新日期：2011-08-01 00:00:00

abstract：:A cytoplasmic NADH oxidase (NOX) was purified from a soil bacteria, Brevibacterium sp. KU1309, which is able to grow in the medium containing 2-phenylethanol as the sole source of carbon under an aerobic condition. The enzyme catalyzed the oxidation of NADH to NAD+ involving two-electron reduction of O2 to H2O2. The m...

journal_title：Applied microbiology and biotechnology

authors： Hirano J,Miyamoto K,Ohta H

更新日期：2008-08-01 00:00:00

abstract：:We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region ...

journal_title：Applied microbiology and biotechnology

authors： Piret J,Resendiz B,Mahro B,Zhang JY,Serpe E,Romero J,Connors N,Demain AL

更新日期：1990-02-01 00:00:00