Production of recombinant human erythropoietin in Bowes melanoma cells in suspension culture.

abstract：:Fermentative H(2) production microbial structure in an agitated granular sludge bed bioreactor was analyzed using fluorescence in situ hybridization (FISH) and polymerase chain reaction-denatured gradient gel electrophoresis (PCR-DGGE). This hydrogen-producing system was operated at four different hydraulic retention ...

journal_title：Applied microbiology and biotechnology

authors： Hung CH,Lee KS,Cheng LH,Huang YH,Lin PJ,Chang JS

更新日期：2007-06-01 00:00:00

abstract：:Fungi can grow in extreme habitats, such as natural stone and mineral building materials, sometimes causing deterioration. Efflorescence-concentrated salt deposits-results from water movement through building material; it can damage masonry materials and other bricks. Fungal isolate KUR1, capable of growth on, and dis...

journal_title：Applied microbiology and biotechnology

authors： Masaphy S,Lavi I,Sultz S,Zabari L

更新日期：2014-06-01 00:00:00

abstract：:The kinetics of growth, acid and solvent production in batch culture of Clostridium pasteurianum DSMZ 525 were examined in mixed or mono-substrate fermentations. In pH-uncontrolled batch cultures, the addition of butyric acid or glucose significantly enhanced n-butanol production and the ratio of butanol/1,3-propanedi...

journal_title：Applied microbiology and biotechnology

authors： Sabra W,Groeger C,Sharma PN,Zeng AP

更新日期：2014-05-01 00:00:00

abstract：:Process intensification and integration is crucial regarding an ever increasing pressure on manufacturing costs and capacities in biologics manufacturing. For virus production in perfusion mode, membrane-based alternating tangential flow filtration (ATF) and acoustic settler are the commonly described cell retention t...

journal_title：Applied microbiology and biotechnology

authors： Gränicher G,Coronel J,Trampler F,Jordan I,Genzel Y,Reichl U

更新日期：2020-06-01 00:00:00

abstract：:Chemical mutagenesis of the phototrophic green microalga Parietochloris incisa, producing high amounts of arachidonic acid (ARA), resulted in selection of a mutant deficient in ARA and rich in dihomo-γ-linolenic acid (DGLA) and thus ∆5 desaturase defective. The mutagenesis produced a nonsense mutation in the ∆5 desatu...

journal_title：Applied microbiology and biotechnology

authors： Iskandarov U,Khozin-Goldberg I,Cohen Z

更新日期：2011-04-01 00:00:00

abstract：:We have constructed a chimeric protein composed of the receptor binding and membrane translocation domains of Pseudomonas exotoxin A (PE) with the outer membrane proteins I and F, together designated as PEIF. The potential of PEIF as a vaccine against Pseudomonas infection was evaluated in BALB/c mice and New Zealand ...

journal_title：Applied microbiology and biotechnology

authors： Chen TY,Shang HF,Chen TL,Lin CP,Hui CF,Hwang J

更新日期：1999-10-01 00:00:00

abstract：:A system for the controlled expression of a foreign gene in cultured tobacco cells (Nicotiana tabacum, BY2) by temperature shift was constructed. A 925-base-pair (bp) DNA fragment containing the 5'-flanking region of a low-molecular-mass heat-shock protein gene (HSP18.2) of Arabidopsis thaliana was inserted upstream o...

journal_title：Applied microbiology and biotechnology

authors： Yoshida K,Kasai T,Garcia MR,Sawada S,Shoji T,Shimizu S,Yamazaki K,Komeda Y,Shinmyo A

更新日期：1995-12-01 00:00:00

abstract：:Recombinant adeno-associated virus (rAAV) vectors are increasingly popular tools for gene therapy applications. Their non-pathogenic status, low inflammatory potential, availability of viral serotypes with different tissue tropisms, and prospective long-lasting gene expression are important attributes that make rAAVs ...

journal_title：Applied microbiology and biotechnology

authors： Aponte-Ubillus JJ,Barajas D,Peltier J,Bardliving C,Shamlou P,Gold D

更新日期：2018-02-01 00:00:00

abstract：:In bioelectrochemical systems, exoelectrogenic bacteria respire with anode electrodes as their extracellular electron acceptor; therefore, lower anode potentials can reduce the energy gain to each microbe and select against ones that are not able to respire at a lower potential range. Often fully developed anode commu...

journal_title：Applied microbiology and biotechnology

authors： Yan H,Yates MD,Regan JM

更新日期：2015-11-01 00:00:00

abstract：:Magnetotactic bacteria are difficult to grow under defined conditions in culture, which has presented a major obstacle to commercial application of magnetosomes. We studied the relationships among the cell growth, magnetosome formation, dissolved oxygen concentration (DO), and the ability to supply oxygen to the cells...

journal_title：Applied microbiology and biotechnology

authors： Sun JB,Zhao F,Tang T,Jiang W,Tian JS,Li Y,Li JL

更新日期：2008-06-01 00:00:00

abstract：:Cultivation of Aspergillus terreus ATCC 20542 in a stirred tank bioreactor was performed to induce the biosynthesis of secondary metabolites and provide the bioprocess-related insights into the metabolic capabilities of the investigated strain. The activation of biosynthetic routes was attempted by the diversification...

journal_title：Applied microbiology and biotechnology

authors： Boruta T,Bizukojc M

更新日期：2016-04-01 00:00:00

abstract：:Escherichia coli K-12 was cultured under anaerobic conditions to form biofilm on carbon fiber electrodes in glucose-containing medium. The anodic current increased with the development of the biofilm and depended on the glucose concentration. Cyclic voltammetric results support the presence of a redox compound(s) excr...

journal_title：Applied microbiology and biotechnology

authors： Wang YF,Tsujimura S,Cheng SS,Kano K

更新日期：2007-10-01 00:00:00

abstract：:Microbial genome sequencing has left a legacy of annotated yet uncharacterized genes or open reading frames, activities that may have useful applications in health and/or the environment. We are interested in the discovery and characterization of potentially new pectinolytic activities for the enzymatic retting of nat...

journal_title：Applied microbiology and biotechnology

authors： Xiao Z,Boyd J,Grosse S,Beauchemin M,Coupe E,Lau PC

更新日期：2008-04-01 00:00:00

abstract：:Phomopsis sp. XP-8 is an endophytic fungus that has the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application for the biosynthesis of PDG independent of plants. When cultivated in mung bean medium, PDG production was significantly improved and pinoresinol monoglucoside (PMG) and ...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Shi J,Gao Z,Yangwu R,Jiang H,Che J,Liu Y

更新日期：2015-06-01 00:00:00

abstract：:DNA polymerase I gene was cloned and sequenced from the thermophilic bacterium Geobacillus caldoxylosilyticus TK4. The gene is 2,634 bp long and encodes a protein of 878 amino acids in length. The enzyme has a molecular mass of 99 kDa and shows sequence homology with DNA polymerase I from Bacillus species (89% identit...

journal_title：Applied microbiology and biotechnology

authors： Sandalli C,Singh K,Modak MJ,Ketkar A,Canakci S,Demir I,Belduz AO

更新日期：2009-08-01 00:00:00

abstract：:We are beginning to see how the microbiota of the human gastrointestinal tract (GIT) can drive the development of new products to benefit human health and wellbeing. Despite the growing market for prebiotics and probiotics, there are currently no commercial products available that aid or increase the attachment of hea...

journal_title：Applied microbiology and biotechnology

authors： Morrin ST,Hickey RM

更新日期：2020-02-01 00:00:00

abstract：:Grasslands in semi-arid Northern China are widely desertified, thus inducing the formation of a large area of moving sand lands. Revegetation of the sandy land is commonly adopted to restore degraded grasslands. The structure of the soil microbial community might dramatically change during degradation and recovery bec...

journal_title：Applied microbiology and biotechnology

authors： Cao C,Zhang Y,Cui Z,Feng S,Wang T,Ren Q

更新日期：2017-08-01 00:00:00

abstract：:γ-Aminobutyric acid (GABA) has been reported to play a neurotransmitter in the central nervous system thereby exerting an inhibition in nerve impulse, in turn ameliorating depression; in addition, recent study also reveals the anti-hypertensive effect of GABA in vivo. Edible fungi of the Monascus species have been use...

journal_title：Applied microbiology and biotechnology

authors： Lee BH,Pan TM

更新日期：2012-06-01 00:00:00

abstract：:Five yeast strains, Saccharomyces cerevisiae D8, M12, and S13; Hanseniaspora uvarum S6; and Issatchenkia orientalis KMBL5774, isolated from Korean grapes, were entrapped in Ca-alginate beads, which are non-toxic, simple to use, and economical. Ca-alginate beads containing yeast cells were soaked in protective solution...

journal_title：Applied microbiology and biotechnology

authors： Kim DH,Lee SB,Park HD

更新日期：2017-01-01 00:00:00

abstract：:Growth parameters, ligninolytic enzyme activities and ability to degrade polycyclic aromatic hydrocarbons by the fungus Irpex lacteus were characterized and compared with those of other white rot fungi capable of rapid decolorization of poly R-478 and Remazol Brilliant Blue R dyes. I. lacteus was able to grow on miner...

journal_title：Applied microbiology and biotechnology

authors： Novotný C,Erbanová P,Cajthaml T,Rothschild N,Dosoretz C,Sasek V

更新日期：2000-12-01 00:00:00

abstract：:Microalgal starch is a potential feedstock for biofuel production. Nutrient stress is widely used to stimulate starch accumulation in microalgae. Cell growth and starch accumulation in the marine green microalga Tetraselmis subcordiformis were evaluated under extracellular phosphorus deprivation with initial cell dens...

journal_title：Applied microbiology and biotechnology

authors： Yao CH,Ai JN,Cao XP,Xue S

更新日期：2013-07-01 00:00:00

abstract：:Plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target of a range of synthetic β-triketone herbicides that are currently used commercially. Their mode of action is based on an irreversible inhibition of HPPD. Therefore, this inhibitory capacity was used to develop a whole-cell colorimetric bioassay w...

journal_title：Applied microbiology and biotechnology

authors： Rocaboy-Faquet E,Noguer T,Romdhane S,Bertrand C,Dayan FE,Barthelmebs L

更新日期：2014-08-01 00:00:00

abstract：:The production of infective juvenile stages (IJ) of the entomopathogenic nematode Steinernema carpocapsae in the presence of its symbiotic bacterium Xenorhabdus nematophilus was carried out in orbitally agitated bottles. Four complex culture media (M1-M4) were used, containing from 8% to 28% (by vol.) agave juice (agu...

journal_title：Applied microbiology and biotechnology

authors： Islas-López MA,Sanjuan-Galindo R,Rodríguez-Hernández AI,Chavarría-Hernández N

更新日期：2005-07-01 00:00:00

abstract：:We have fused the epidermal growth factor (EGF) to the amino terminus of Pseudomonas exotoxin A (PE) to create a cytotoxic agent, designated EGF-PE, which preferentially kills EGF-receptor-bearing cells. In this study, we analyzed the effect of the Ia domain, the binding domain of PE on the cytotoxicity of EGF-PE towa...

journal_title：Applied microbiology and biotechnology

authors： Liao CW,Hseu TH,Hwang J

更新日期：1995-07-01 00:00:00

abstract：:The ligninolytic enzymes of white-rot fungi have a broad substrate specificity and have been implicated in the transformation and mineralization of organopollutants with structural similarities to lignin. This review presents evidence for the involvement of these enzymes in white-rot fungal degradation of munitions wa...

journal_title：Applied microbiology and biotechnology

authors： Pointing SB

更新日期：2001-10-01 00:00:00

abstract：:A gratuitous strain was developed by disrupting the GAL1 gene (galactokinase) of recombinant Saccharomyces cerevisiae harboring the antithrombotic hirudin gene in the chromosome under the control of the GAL10 promoter. A series of glucose-limited fed-batch cultures were carried out to examine the effects of glucose su...

journal_title：Applied microbiology and biotechnology

authors： Kim MD,Lee TH,Lim HK,Seo JH

更新日期：2004-08-01 00:00:00

abstract：:In industry, filamentous fungi have a prominent position as producers of economically relevant primary or secondary metabolites. Particularly, the advent of genetic engineering of filamentous fungi has led to a growing number of molecular tools to adopt filamentous fungi for biotechnical applications. Here, we summari...

journal_title：Applied microbiology and biotechnology

authors： Kluge J,Terfehr D,Kück U

更新日期：2018-08-01 00:00:00

abstract：:The efficient production of recombinant proteins such as antibodies typically involves the screening of an extravagant number of clones in order to finally select a stable and high-producing cell line. Thereby, the underlying principles of a powerful protein machinery, but also potential expression limitations, often ...

journal_title：Applied microbiology and biotechnology

authors： Reinhart D,Sommeregger W,Debreczeny M,Gludovacz E,Kunert R

更新日期：2014-07-01 00:00:00

abstract：:Fluoroaromatics are widely and--in recent years--increasingly used as agrochemicals, starting materials for chemical syntheses and especially pharmaceuticals. This originates from the special properties the carbon-fluorine bond is imposing on organic molecules. Hence, fluoro-substituted compounds more and more are con...

journal_title：Applied microbiology and biotechnology

authors： Kiel M,Engesser KH

更新日期：2015-09-01 00:00:00

abstract：:Most recombinant proteins in Escherichia coli are not efficiently secreted to the extracellular space. Structural stabilisation of the cell wall is essential for extracellular protein production in E. coli, for which D,D-carboxypeptidases are essential. Herein, we perturbed the peptidoglycan structure of the E. coli c...

journal_title：Applied microbiology and biotechnology

authors： Yang H,Hu J,Lu X,Wang F,Shen W,Hu W,Wang L,Chen X,Liu L

更新日期：2019-01-01 00:00:00