当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Effect of air-blast drying and the presence of protectants on the viability of yeast entrapped in calcium alginate beads with an aim to improve the survival rate.

Effect of air-blast drying and the presence of protectants on the viability of yeast entrapped in calcium alginate beads with an aim to improve the survival rate.

Abstract：

:Five yeast strains, Saccharomyces cerevisiae D8, M12, and S13; Hanseniaspora uvarum S6; and Issatchenkia orientalis KMBL5774, isolated from Korean grapes, were entrapped in Ca-alginate beads, which are non-toxic, simple to use, and economical. Ca-alginate beads containing yeast cells were soaked in protective solutions, such as skim milk, saccharides, polyols, and nitrogen compounds, before air-blast drying to improve the yeast survival rate and storage ability. The results showed that both entrapment in Ca-alginate beads and soaking in protective agents favorably affected the survival of all strains. The microenvironment formed by the beads and protective agents can protect the yeast cells from harsh environmental conditions, such as low water (below 10 %). All the yeast strains entrapped in Ca-alginate beads showed greater than 80 % survival and less than 11 % water content after air-blast drying at 37 °C for 5 h. In addition, air-blast dried cells of S. cerevisiae D8, M12, S13; H. uvarum S6; and I. orientalis KMBL5774 entrapped in 2 % Ca-alginate beads and soaked in protective agents (10 % skim milk containing 10 % sucrose, 10 % raffinose, 10 % trehalose, 10 % trehalose, and 10 % glucose, respectively) after air-blast drying at 37 °C for 5 h showed 90, 87, 92, 90, and 87 % viability, respectively. All dried entrapped yeast cells showed survival rates of at least 51 % after storage at 4 °C for 3 months.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Kim DH,Lee SB,Park HD

doi

10.1007/s00253-016-7744-9

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

93-102

issue

1

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-016-7744-9

journal_volume

101

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • Understanding the mechanistic roles of 30Kc6 gene in apoptosis and specific productivity in antibody-producing Chinese hamster ovary cells.

    abstract：:Previously, we reported that the expression of Bombyx mori 30Kc6 gene in Chinese hamster ovary (CHO) cells increases recombinant protein production by both inhibiting apoptosis and enhancing specific productivity. In this study, in order to gain a thorough understanding of the roles of 30Kc6 gene in antibody productio...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-3899-1

    authors： Wang Z,Ma X,Fan L,Rhee WJ,Park TH,Zhao L,Tan WS

    更新日期：2012-06-01 00:00:00

  • Development and validation of a new dynamic in vitro model of the piglet colon (PigutIVM): application to the study of probiotics.

    abstract：:For ethical, technical, regulatory, and cost reasons, in vitro methods are increasingly used as an alternative to in vivo experimentations. The aim of the present study was to validate, according to in vivo data in living animals, a new in vitro model of the piglet colon, the PigutIVM, under both control conditions an...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-017-8122-y

    authors： Fleury MA,Le Goff O,Denis S,Chaucheyras-Durand F,Jouy E,Kempf I,Alric M,Blanquet-Diot S

    更新日期：2017-03-01 00:00:00

  • Polyketides in Aspergillus terreus: biosynthesis pathway discovery and application.

    abstract：:The knowledge of biosynthesis gene clusters, production improving methods, and bioactivity mechanisms is very important for the development of filamentous fungi metabolites. Metabolic engineering and heterologous expression methods can be applied to improve desired metabolite production, when their biosynthesis pathwa...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-016-7733-z

    authors： Yin Y,Cai M,Zhou X,Li Z,Zhang Y

    更新日期：2016-09-01 00:00:00

  • Structural and physicochemical properties of polar lipids from thermophilic archaea.

    abstract：:The essential general features required for lipid membranes of extremophilic archaea to fulfill biological functions are that they are in the liquid crystalline phase and have extremely low permeability of solutes that is much less temperature sensitive due to a lack of lipid-phase transition and highly branched isopr...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-009-2102-9

    authors： Ulrih NP,Gmajner D,Raspor P

    更新日期：2009-08-01 00:00:00

  • Secretory production system of bionanocapsules using a stably transfected insect cell line.

    abstract：:Bionanocapsules (BNCs) are hollow nanoscale particles composed of L protein of the hepatitis B virus surface antigen that represent specific affinity for human hepatocytes. BNCs can transfer genes and drugs into human hepatocytes efficiently and specifically. BNC can be expressed in yeast cells. In this study, we deve...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0486-3

    authors： Shishido T,Muraoka M,Ueda M,Seno M,Tanizawa K,Kuroda S,Fukuda H,Kondo A

    更新日期：2006-12-01 00:00:00

  • Impact of protein uptake and degradation on recombinant protein secretion in yeast.

    abstract：:Protein titers, a key bioprocessing metric, depend both on the synthesis of protein and the degradation of protein. Secreted recombinant protein production in Saccharomyces cerevisiae is an attractive platform as minimal media can be used for cultivation, thus reducing fermentation costs and simplifying downstream pur...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-5783-7

    authors： Tyo KE,Liu Z,Magnusson Y,Petranovic D,Nielsen J

    更新日期：2014-08-01 00:00:00

  • An overview of microbial indigo-forming enzymes.

    abstract：:Indigo is one of the oldest textile dyes and was originally prepared from plant material. Nowadays, indigo is chemically synthesized at a large scale to satisfy the demand for dyeing jeans. The current indigo production processes are based on fossil feedstocks; therefore, it is highly attractive to develop a more sust...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-019-10292-5

    authors： Fabara AN,Fraaije MW

    更新日期：2020-02-01 00:00:00

  • Metabolic engineering of Saccharomyces cerevisiae for production of novel lipid compounds.

    abstract：:The yeast Saccharomyces cerevisiae has been modified successfully for production of numerous metabolites and therapeutic proteins through metabolic engineering, but has not been utilized to date for the production of lipid-derived compounds. We developed a lipid metabolic engineering strategy in S. cerevisiae based up...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-002-0997-5

    authors： Dyer JM,Chapital DC,Kuan JW,Mullen RT,Pepperman AB

    更新日期：2002-07-01 00:00:00

  • Comparison of green fluorescent protein expression in two industrial Escherichia coli strains, BL21 and W3110, under co-expression of bacterial hemoglobin.

    abstract：:Vitreoscilla hemoglobin (VHb) has been successfully used to enhance production of foreign proteins in several microorganisms including Escherichia coli. We compared the expression of an oxygen-dependent foreign protein, green fluorescent protein (GFP) under co-expression of VHb in two typical industrial E. coli strain...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-002-1043-3

    authors： Kang DG,Kim YK,Cha HJ

    更新日期：2002-08-01 00:00:00

  • Continuous 2-keto-L-gulonic acid fermentation from L-sorbose by Ketogulonigenium vulgare DSM 4025.

    abstract：:A single-stage continuous fermentation process for the production of 2-keto-L-gulonic acid (2KGA) from L-sorbose using Ketogulonigenium vulgare DSM 4025 was developed. The chemostat culture with the dilution rate that was calculated based on the relationship between the 2KGA production rate and the 2KGA concentration ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1822-6

    authors： Takagi Y,Sugisawa T,Hoshino T

    更新日期：2009-04-01 00:00:00

  • Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium.

    abstract：:Lactococcus lactis is the most important starter culture organism used in the dairy industry. Although L. lactis species have been awarded Qualified Presumption of Safety status by the European Food Safety Authority, and Generally Regarded as Safe status by the US Food and Drug Administration, some strains can produce...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-6130-8

    authors： del Rio B,Linares DM,Ladero V,Redruello B,Fernández M,Martin MC,Alvarez MA

    更新日期：2015-01-01 00:00:00

  • Medicinal components in Termitomyces mushrooms.

    abstract：:Termitomyces is a genus of edible mushrooms commonly consumed in Africa and Asia among the mushrooms collected from the wild. Termitomyces mushrooms grow as symbionts in the termite nests, where they produce various enzymes to help termites digest lignocellulosic substrates. Many species of Termitomyces are used by di...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-018-8991-8

    authors： Hsieh HM,Ju YM

    更新日期：2018-06-01 00:00:00

  • Strategies to prevent the occurrence of resistance against antibiotics by using advanced materials.

    abstract：:Drug resistance occurrence is a global healthcare concern responsible for the increased morbidity and mortality in hospitals, time of hospitalisation and huge financial loss. The failure of the most antibiotics to kill "superbugs" poses the urgent need to develop innovative strategies aimed at not only controlling bac...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-018-8776-0

    authors： Bassegoda A,Ivanova K,Ramon E,Tzanov T

    更新日期：2018-03-01 00:00:00

  • Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes.

    abstract：:For the biotechnological production of L: -lysine, mainly strains of Corynebacterium glutamicum are used, which have been obtained by classical mutagenesis and screening or selection or by metabolic engineering. Gene targets for the amplification and deregulation of the lysine biosynthesis pathway, for the improvement...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-0916-x

    authors： Sindelar G,Wendisch VF

    更新日期：2007-09-01 00:00:00

  • Metal-induced inhibition of anaerobic metabolism of volatile fatty acids and hydrogen.

    abstract：:The effects of copper (Cu), chromium (Cr), cadmium (Cd), lead (Pb) and zinc (Zn) on the biotransformation of organic acids (acetate, propionate and butyrate) and H2 were assessed in serum-bottle microcosms. Experiments were performed over a range of metal concentrations (20-200 mg/l) using biomass from an anaerobic bi...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00902748

    authors： Kong IC,Hubbard JS,Jones WJ

    更新日期：1994-11-01 00:00:00

  • Effects of constant or dynamic low anode potentials on microbial community development in bioelectrochemical systems.

    abstract：:In bioelectrochemical systems, exoelectrogenic bacteria respire with anode electrodes as their extracellular electron acceptor; therefore, lower anode potentials can reduce the energy gain to each microbe and select against ones that are not able to respire at a lower potential range. Often fully developed anode commu...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6907-4

    authors： Yan H,Yates MD,Regan JM

    更新日期：2015-11-01 00:00:00

  • Hemidesmus indicus L. Br.: critical assessment of in vitro biotechnological advancements and perspectives.

    abstract：:Hemidesmus indicus (L.) R. Br. ex Schult., commonly known as Indian sarsaparilla or Anantamul, is an ethnopharmacologically important medicinal plant from the family Apocynaceae. Biosynthesis of an array of bioactive compounds such as methoxybenzaldehydes and their derivatives has been accountable for its intensive me...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-020-10851-1

    authors： Nandy S,Singh J,Pandey DK,Dey A

    更新日期：2020-10-01 00:00:00

  • Applications of quorum sensing in biotechnology.

    abstract：:Many unicellular microorganisms use small signaling molecules to determine their local concentration. The processes involved in the production and recognition of these signals are collectively known as quorum sensing (QS). This form of cell-cell communication is used by unicellular microorganisms to co-ordinate their ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-010-2521-7

    authors： Choudhary S,Schmidt-Dannert C

    更新日期：2010-05-01 00:00:00

  • A genetically modified solvent-tolerant bacterium for optimized production of a toxic fine chemical.

    abstract：:The aim of the study was to investigate whether toxic fine chemical production can be improved using the solvent-tolerant Pseudomonas putida S12 in a two-liquid-phase system consisting of aqueous media and a water-immiscible octanol phase with production of 3-methylcatechol from toluene as the model conversion. For th...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530000381

    authors： Wery J,Mendes da Silva DI,de Bont JA

    更新日期：2000-08-01 00:00:00

  • Production of xylanase by an alkaline-tolerant marine-derived Streptomyces viridochromogenes strain and improvement by ribosome engineering.

    abstract：:Xylanase is the enzyme complex that is responsible for the degradation of xylan; however, novel xylanase producers remain to be explored in marine environment. In this study, a Streptomyces strain M11 which exhibited xylanase activity was isolated from marine sediment. The 16S rDNA sequence of M11 showed the highest i...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4290-y

    authors： Liu Z,Zhao X,Bai F

    更新日期：2013-05-01 00:00:00

  • Isolation and lipid degradation profile of Raoultella planticola strain 232-2 capable of efficiently catabolizing edible oils under acidic conditions.

    abstract：:The lipids (fats and oils) degradation capabilities of soil microorganisms were investigated for possible application in treatment of lipids-contaminated wastewater. We isolated a strain of the bacterium Raoultella planticola strain 232-2 that is capable of efficiently catabolizing lipids under acidic conditions such ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-3982-7

    authors： Sugimori D,Watanabe M,Utsue T

    更新日期：2013-01-01 00:00:00

  • Two novel cyclic hexapeptides from the genetically engineered Actinosynnema pretiosum.

    abstract：:Two novel cyclic hexapeptides designated actinosynneptides A (1) and B (2), together with three tryptophan containing diketopiperazines, namely cyclo(L-Trp-L-Trp) (3), cyclo(L-Trp-N-MeL-Trp) (4), and cyclo(N-MeL-Trp-N-MeL-Trp) (5), were isolated from the culture of the genetically engineered strain HGF052::asm18 deriv...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-8017-3

    authors： Lu C,Xie F,Shan C,Shen Y

    更新日期：2017-03-01 00:00:00

  • Bacterial glucans: production, properties, and applications.

    abstract：:Bacterial glucans have aroused increasing interest in commercial applications in the food and pharmaceutical sectors. A number of bacterial glucans have been reported over recent decades, and their structure, production, and functional properties have been extensively studied. In this paper, we review recent researche...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-016-7836-6

    authors： Xu L,Zhang J

    更新日期：2016-11-01 00:00:00

  • Production of 7, 10-dihydroxy-8(E)-octadecenoic acid from triolein via lipase induction by Pseudomonas aeruginosa PR3.

    abstract：:Hydroxy fatty acids (HFA) have gained importance because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. The bacterial isolate Pseudomonas aeruginosa (PR3) was reported to produce mono-, di-, and trihydroxy fatty acids from different unsaturated fatty ac...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0662-5

    authors： Chang IA,Kim IH,Kang SC,Hou CT,Kim HR

    更新日期：2007-02-01 00:00:00

  • Irpex lacteus, a white rot fungus applicable to water and soil bioremediation.

    abstract：:Growth parameters, ligninolytic enzyme activities and ability to degrade polycyclic aromatic hydrocarbons by the fungus Irpex lacteus were characterized and compared with those of other white rot fungi capable of rapid decolorization of poly R-478 and Remazol Brilliant Blue R dyes. I. lacteus was able to grow on miner...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530000432

    authors： Novotný C,Erbanová P,Cajthaml T,Rothschild N,Dosoretz C,Sasek V

    更新日期：2000-12-01 00:00:00

  • Effect of 1,3-propanediol, organic acids, and ethanol on growth and metabolism of Clostridium butyricum DSP1.

    abstract：:Knowledge of tolerance of bacteria to toxic stress is important, especially for processes targeted at high final titers of product. Information on environmental limits and stress responses may help during selection of strains or design and control of processes. The influence of the main product and its co-products on ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-6292-4

    authors： Szymanowska-Powałowska D,Kubiak P

    更新日期：2015-04-01 00:00:00

  • Generalizing a hybrid synthetic promoter approach in Yarrowia lipolytica.

    abstract：:Both varied and strong promoters are essential for metabolic and pathway engineering applications in any host organism. To enable this capacity, here we demonstrate a generalizable method for the de novo construction of strong, synthetic hybrid promoter libraries. Specifically, we demonstrate how promoter truncation a...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4421-5

    authors： Blazeck J,Reed B,Garg R,Gerstner R,Pan A,Agarwala V,Alper HS

    更新日期：2013-04-01 00:00:00

  • A mutant trypsin-like enzyme from Streptomyces fradiae, created by site-directed mutagenesis, improves affinity chromatography for protein trypsin inhibitors.

    abstract：:The Ser-170 residue of a trypsin-like enzyme from Streptomyces fradiae (SFT), which is considered to be the active-site serine, was replaced with alanine by site-directed mutagenesis to improve the affinity chromatography step for a Kazal-type trypsin inhibitor pancreatic secretory trypsin inhibitor (PSTI). The result...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530050777

    authors： Katoh T,Kikuchi N,Nagata K,Yoshida N

    更新日期：1996-08-01 00:00:00

  • Linking seasonal inorganic nitrogen shift to the dynamics of microbial communities in the Chesapeake Bay.

    abstract：:Seasonal shifts of dissolved inorganic nitrogen (DIN) and the dynamics of microbial communities for nitrogen transformation were investigated in the water column of Chesapeake Bay. The relative abundance of nitrogen over phosphorus (N) showed a strong seasonal and spatial pattern: gradually decreased from upstream to ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-5337-4

    authors： Hong Y,Xu X,Kan J,Chen F

    更新日期：2014-04-01 00:00:00

  • Sequence of the Corynebacterium glutamicum pyruvate carboxylase gene.

    abstract：:Pyruvate carboxylase is an important anaplerotic enzyme replenishing oxaloacetate consumed for biosynthesis during growth, or lysine and glutamic acid production in industrial fermentations. We used regions of homology from pyruvate carboxylase sequences of 12 different species (corresponding to the ATP- and pyruvate-...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530051302

    authors： Koffas MA,Ramamoorthi R,Pine WA,Sinskey AJ,Stephanopoulos G

    更新日期：1998-09-01 00:00:00