当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Improved n-butanol production by a non-acetone producing Clostridium pasteurianum DSMZ 525 in mixed substrate fermentation.

Improved n-butanol production by a non-acetone producing Clostridium pasteurianum DSMZ 525 in mixed substrate fermentation.

Abstract：

:The kinetics of growth, acid and solvent production in batch culture of Clostridium pasteurianum DSMZ 525 were examined in mixed or mono-substrate fermentations. In pH-uncontrolled batch cultures, the addition of butyric acid or glucose significantly enhanced n-butanol production and the ratio of butanol/1,3-propanediol. In pH-controlled batch culture at pH = 6, butyric acid addition had a negative effect on growth and did not lead to a higher n-butanol productivity. On the other hand, mixed substrate fermentation using glucose and glycerol enhanced the growth and acid production significantly. Glucose limitation in the mixed substrate fermentation led to the reduction or inhibition of the glycerol consumption by the growing bacteria. Therefore, for the optimal growth and n-butanol production by C. pasteurianum, a limitation of either substrate should be avoided. Under optimized batch conditions, n-butanol concentration and maximum productivity achieved were 21 g/L, and 0.96 g/L × h, respectively. In comparison, mixed substrate fermentation using biomass hydrolysate and glycerol gave a n-butanol concentration of 17 g/L with a maximum productivity of 1.1 g/L × h. In terms of productivity and final n-butanol concentration, the results demonstrated that C. pasteurianum DSMZ 525 is well suitable for n-butanol production from mixed substrates of biomass hydrolysate and glycerol and represents an alternative promising production strain.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Sabra W,Groeger C,Sharma PN,Zeng AP

doi

10.1007/s00253-014-5588-8

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

4267-76

issue

9

eissn

0175-7598

issn

1432-0614

journal_volume

98

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • The antibacterial and immunostimulative effect of chitosan-oligosaccharides against infection by Staphylococcus aureus isolated from bovine mastitis.

    abstract：:Based on our previous study evaluating the in vivo cure efficacy of chitosan on bovine mastitis, a more water-soluble chitosan-oligosaccharide (OCHT) with a high degree of deacetylation and low molecular weight was prepared to obtain high antibiotic efficacy. The growth of Staphylococcus aureus isolated from bovine ma...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-0898-8

    authors： Moon JS,Kim HK,Koo HC,Joo YS,Nam HM,Park YH,Kang MI

    更新日期：2007-07-01 00:00:00

  • A 1-year study of the activities of seven hydrolases in a communal wastewater treatment plant: trends and correlations.

    abstract：:The activities of seven hydrolytic enzymes (L-alanine aminopeptidase, esterase, α-and β-glucosidase, phosphomonoesterase, phosphodiesterase, sulfatase) were monitored during 1 year in parallel and serial treatment units of the biological stage of a communal wastewater treatment plant. The spatial homogeneity of enzyme...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7540-6

    authors： Kreutz JA,Böckenhüser I,Wacht M,Fischer K

    更新日期：2016-08-01 00:00:00

  • Combination of aquifer thermal energy storage and enhanced bioremediation: resilience of reductive dechlorination to redox changes.

    abstract：:To meet the demand for sustainable energy, aquifer thermal energy storage (ATES) is widely used in the subsurface in urban areas. However, contamination of groundwater, especially with chlorinated volatile organic compounds (CVOCs), is often being encountered. This is commonly seen as an impediment to ATES implementat...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-7241-6

    authors： Ni Z,van Gaans P,Smit M,Rijnaarts H,Grotenhuis T

    更新日期：2016-04-01 00:00:00

  • Methyl-branched poly(hydroxyalkanoate) biosynthesis from 13-methyltetradecanoic acid and mixed isostearic acid isomer substrates.

    abstract：:Pseudomonas resinovorans, a known medium-chain-length (mcl-) poly(hydroxyalkanoate) (PHA) producer, was grown on 13-methyltetradecanoic acid (13-MTDA) and a mixture of isostearic acid (IA) isomers to produce methyl-branched mcl-PHA polymers. Shake-flask experiments revealed polymer productivities (the percent of the c...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-009-2134-1

    authors： Ashby RD,Ngo HL,Solaiman DK,Strahan G

    更新日期：2009-11-01 00:00:00

  • Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation.

    abstract：:Saccharomyces cerevisiae was transformed with the Pichia stipitis CBS 6054 XYL1 and XYL2 genes encoding xylose reductase (XR) and xylitol dehydrogenase (XDH) respectively. The XYL1 and XYL2 genes were placed under the control of the alcohol dehydrogenase 1 (ADH1) and phosphoglycerate kinase (PGK1) promoters in the yea...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530051041

    authors： Walfridsson M,Anderlund M,Bao X,Hahn-Hägerdal B

    更新日期：1997-08-01 00:00:00

  • Reduction of volatile acidity of acidic wines by immobilized Saccharomyces cerevisiae cells.

    abstract：:Excessive volatile acidity in wines is a major problem and is still prevalent because available solutions are nevertheless unsatisfactory, namely, blending the filter-sterilized acidic wine with other wines of lower volatile acidity or using reverse osmosis. We have previously explored the use of an empirical biologic...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-4719-y

    authors： Vilela A,Schuller D,Mendes-Faia A,Côrte-Real M

    更新日期：2013-06-01 00:00:00

  • Pseudomonas aeruginosa ATCC 9027 is a non-virulent strain suitable for mono-rhamnolipids production.

    abstract：:Rhamnolipids produced by Pseudomonas aeruginosa are biosurfactants with a high biotechnological potential, but their extensive commercialization is limited by the potential virulence of P. aeruginosa and by restrictions in producing these surfactants in heterologous hosts. In this work, we report the characterization ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7789-9

    authors： Grosso-Becerra MV,González-Valdez A,Granados-Martínez MJ,Morales E,Servín-González L,Méndez JL,Delgado G,Morales-Espinosa R,Ponce-Soto GY,Cocotl-Yañez M,Soberón-Chávez G

    更新日期：2016-12-01 00:00:00

  • Study on expression and action mode of recombinant alginate lyases based on conserved domains reconstruction.

    abstract：:Understanding the effect of conserved domains reconstruction of alginate lyases on action mode is essential for their application and in-depth study. We report the expression and action mode of recombinant alginate lyase (AlyM) and its conserved domain reconstruction forms (AlyMΔCBM, cAlyM, and AlyMΔ58C). The enzymati...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-018-9502-7

    authors： Yang M,Li N,Yang S,Yu Y,Han Z,Li L,Mou H

    更新日期：2019-01-01 00:00:00

  • Genome-centered omics insight into the competition and niche differentiation of Ca. Jettenia and Ca. Brocadia affiliated to anammox bacteria.

    abstract：:Although the niche differentiation of anammox bacteria has been extensively observed in a lab-scale reactor, the inherent mechanism for this ecological phenomenon is still elusive. Here, we combined the long-term reactor operation, genome-centered metagenome, and metatranscriptome analyses to gain insight into the sub...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-019-10040-9

    authors： Zhao Y,Feng Y,Chen L,Niu Z,Liu S

    更新日期：2019-10-01 00:00:00

  • Production of recombinant mink growth hormone in E. coli.

    abstract：:Escherichia coli cells expressing mink (Mustela vison) growth hormone were grown in a batch fermentation process. The expression level was estimated to be 27% of the total cellular protein after 3 h of induction with 1 mM isopropyl beta-D-thiogalactoside (IPTG). If the expression of mink growth hormone (mGH) was induc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0673-2

    authors： Sereikaite J,Statkute A,Morkunas M,Radzevicius K,Borromeo V,Secchi C,Bumelis VA

    更新日期：2007-02-01 00:00:00

  • Anaerobic glucose consumption is accelerated at non-proliferating elevated temperatures through upregulation of a glucose transporter gene in Corynebacterium glutamicum.

    abstract：:Cell proliferation is achieved through numerous enzyme reactions. Temperature governs the activity of each enzyme, ultimately determining the optimal growth temperature. The synthesis of useful chemicals and fuels utilizes a fraction of available metabolic pathways, primarily central metabolic pathways including glyco...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-10739-0

    authors： Uchikura H,Toyoda K,Matsuzawa H,Mizuno H,Ninomiya K,Takahashi K,Inui M,Tsuge Y

    更新日期：2020-08-01 00:00:00

  • Role of viral suppressors governing asymmetric synergism between tomato-infecting begomoviruses.

    abstract：:Mixed viral infections are common in fields and frequently exacerbate disease severity via synergistic interactions among individual viral genomic components leading to major crop loss. Two predominant species of tomato-infecting begomoviruses, Tomato leaf curl New Delhi virus (ToLCNDV) and Tomato leaf curl Gujarat vi...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-11070-4

    authors： Basu S,Singh AK,Singh D,Sahu SK,Chakraborty S

    更新日期：2021-01-08 00:00:00

  • Effect of electron mediators on current generation and fermentation in a microbial fuel cell.

    abstract：:Effects of select electron mediators [9,10-anthraquinone-2,6-disulfonic acid disodium salt (AQDS), safranine O, resazurin, methylene blue, and humic acids] on metabolic end-products and current production from cellulose digestion by Clostridium cellulolyticum in microbial fuel cells (MFCs) were studied using capillary...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1038-1

    authors： Sund CJ,McMasters S,Crittenden SR,Harrell LE,Sumner JJ

    更新日期：2007-09-01 00:00:00

  • Characterization and flocculation mechanism of a bioflocculant from potato starch wastewater.

    abstract：:This study investigated the characterization and flocculation mechanism of a bioflocculant prepared using potato starch wastewater. The optimal culture conditions of this strain were determined as 4 g K2HPO4, 2 g KH2PO4, 0.2 g MgSO4, 0.1 g NaCl, and 2.0 g urea dissolved in 1.0 L potato starch wastewater with no need o...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6589-y

    authors： Guo J,Lau AK,Zhang Y,Zhao J

    更新日期：2015-07-01 00:00:00

  • Current state-of-the-art in the use of plants for the production of recombinant vaccines against infectious bursal disease virus.

    abstract：:Infectious bursal disease is a widely spread threatening contagious viral infection of chickens that induces major damages to the Bursa of Fabricius and leads to severe immunosuppression in young birds causing significant economic losses for poultry farming. The etiological agent is the infectious bursal disease virus...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-020-10397-2

    authors： Rage E,Marusic C,Lico C,Baschieri S,Donini M

    更新日期：2020-03-01 00:00:00

  • Physiological heterogeneity of Pseudomonas taetrolens during lactobionic acid production.

    abstract：:Physiological heterogeneity constitutes a critical parameter in biotechnological systems since both metabolite yield and productivity are often hampered by the presence of undesired physiological cell subpopulations. In the present study, the physiological status and functionality of Pseudomonas taetrolens cells were ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4254-2

    authors： Alonso S,Rendueles M,Díaz M

    更新日期：2012-12-01 00:00:00

  • Antimicrobial activity, cytotoxicity and chemical analysis of lemongrass essential oil (Cymbopogon flexuosus) and pure citral.

    abstract：:The aim of this study was to determine the antimicrobial effects of lemongrass essential oil (C. flexuosus) and to determine cytotoxic effects of both test compounds on human dermal fibroblasts. Antimicrobial susceptibility screening was carried out using the disk diffusion method. Antimicrobial resistance was observe...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7807-y

    authors： Adukwu EC,Bowles M,Edwards-Jones V,Bone H

    更新日期：2016-11-01 00:00:00

  • In vitro evaluation of the fermentation properties of galactooligosaccharides synthesised by alpha-galactosidase from Lactobacillus reuteri.

    abstract：:Stirred, pH-controlled anaerobic batch cultures were used to evaluate the in vitro utilisation by canine gut microflora of novel alpha-galactooligosaccharides synthesised with an enzyme extract from a canine Lactobacillus reuteri strain. Fructooligosaccharides (FOS), melibiose and raffinose were used as reference carb...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-003-1427-z

    authors： Tzortzis G,Goulas AK,Baillon ML,Gibson GR,Rastall RA

    更新日期：2004-03-01 00:00:00

  • An overview of microbial indigo-forming enzymes.

    abstract：:Indigo is one of the oldest textile dyes and was originally prepared from plant material. Nowadays, indigo is chemically synthesized at a large scale to satisfy the demand for dyeing jeans. The current indigo production processes are based on fossil feedstocks; therefore, it is highly attractive to develop a more sust...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-019-10292-5

    authors： Fabara AN,Fraaije MW

    更新日期：2020-02-01 00:00:00

  • Efficient amplification of genes involved in microbial secondary metabolism by an improved genome walking method.

    abstract：:Genome walking is a commonly used technique for the identification of DNA sequences adjacent to known regions. Despite the development of various genome walking methods, nonspecific products are often produced in certain circumstances, especially when GC-rich DNA sequences are dealt with. To effectively resolve such t...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-010-2569-4

    authors： Deng J,Wei M,Yu B,Chen Y

    更新日期：2010-06-01 00:00:00

  • A single-plasmid vector for transgene amplification using short hairpin RNA targeting the 3'-UTR of amplifiable dhfr.

    abstract：:Gene amplification using dihydrofolate reductase gene (dhfr) and methotrexate (MTX) is widely used for recombinant protein production in mammalian cells and is typically conducted in DHFR-deficient Chinese hamster ovary (CHO) cell lines. Generation of DHFR-deficient cells can be achieved by an expression vector incorp...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6856-y

    authors： Kang SY,Kim YG,Lee HW,Lee EG

    更新日期：2015-12-01 00:00:00

  • Microbial transformation of benzene to cis-3,5-cyclohexadien-1,2-diols by recombinant bacteria harboring toluene dioxygenase gene tod.

    abstract：:Toluene dioxygenase (TDO) catalyzes asymmetric cis-dihydroxylation of aromatic compounds. To achieve high efficient biotransformation of benzene to benzene cis-diols, Pseudomonas putida KT2442, Pseudomonas stutzeri 1317, and Aeromonas hydrophila 4AK4 were used as hosts to express TDO gene tod. Plasmid pSPM01, a deriva...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0637-6

    authors： Ouyang SP,Sun SY,Liu Q,Chen J,Chen GQ

    更新日期：2007-02-01 00:00:00

  • The mechanism of bacterial indigo reduction.

    abstract：:The reduction of water-insoluble indigo by the recently isolated moderate thermophile, Clostridium isatidis, has been studied with the aim of developing a sustainable technology for industrial indigo reduction. The ability to reduce indigo was not shared with C. aurantibutyricum, C. celatum and C. papyrosolvens, but C...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-004-1839-4

    authors： Nicholson SK,John P

    更新日期：2005-07-01 00:00:00

  • Endophytic fungi as models for the stereoselective biotransformation of thioridazine.

    abstract：:The stereoselective kinetic biotransformation of thioridazine, a phenothiazine neuroleptic drug, by endophytic fungi was investigated. In general, the sulfur of lateral chain (position 2) or the sulfur of phenothiazinic ring (position 5) were oxidated yielding the major human metabolites thioridazine-2-sulfoxide and t...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1171-x

    authors： Borges KB,Borges Wde S,Pupo MT,Bonato PS

    更新日期：2007-12-01 00:00:00

  • Directed evolution of a Baeyer-Villiger monooxygenase to enhance enantioselectivity.

    abstract：:The Baeyer-Villiger monooxygenase (BVMO) BmoF1 from Pseudomonas fluorescens DSM 50106 was shown before to enantioselectively oxidize different 4-hydroxy-2-ketones to the corresponding hydroxyalkyl acetates, being the first example of a BVMO-catalyzed kinetic resolution of aliphatic acyclic ketones. However, the wild-t...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1646-4

    authors： Kirschner A,Bornscheuer UT

    更新日期：2008-12-01 00:00:00

  • Discovery of a thermostable Baeyer-Villiger monooxygenase by genome mining.

    abstract：:Baeyer-Villiger monooxygenases represent useful biocatalytic tools, as they can catalyze reactions which are difficult to achieve using chemical means. However, only a limited number of these atypical monooxygenases are available in recombinant form. Using a recently described protein sequence motif, a putative Baeyer...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-004-1749-5

    authors： Fraaije MW,Wu J,Heuts DP,van Hellemond EW,Spelberg JH,Janssen DB

    更新日期：2005-01-01 00:00:00

  • Use of Aspergillus overproducing mutants, cured for integrated plasmid, to overproduce heterologous proteins.

    abstract：:Aspergillus niger var. awamori was previously transformed with a vector designed to express a fused glucoamylase-prochymosin gene and bearing the Neurospora crassa pyr4 gene as a selectable marker. Mutant strains that overproduced the glucoamylase-prochymosin fusion protein were derived from one of the transformants. ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00164459

    authors： Ward M,Wilson LJ,Kodama KH

    更新日期：1993-08-01 00:00:00

  • The histone acetyltransferase Mst2 sustains the biological control potential of a fungal insect pathogen through transcriptional regulation.

    abstract：:Histone lysine acetylation orchestrates transcriptional activity essential for diverse cellular events across organisms, but it remains poorly understood how an acetylated lysine affects cellular functions in filamentous fungal pathogens. Here, we show the functions of a histone acetyltransferase that is phylogenetica...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-017-8703-9

    authors： Wang JJ,Cai Q,Qiu L,Ying SH,Feng MG

    更新日期：2018-02-01 00:00:00

  • Biotransformation of dichloroaromatic compounds in nonadapted and adapted freshwater sediment slurries.

    abstract：:Nonadapted freshwater sediment slurries and sediment slurries adapted to dechlorinate 2,3-dichloropyridine (2,3-Cl2Pyd), 2,3-dichloroaniline (2,3-Cl2Anl), 2,3-dichlorophenol (2,3-Cl2PhOH), 3,5-dichloropyridine (3,5-Cl2Pyd), 3,5-dichloroaniline (3,5-Cl2Anl) and 3,5-dichlorophenol (3,5-Cl2PhOH) were studied to determine...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00164780

    authors： Liu SM,Jones WJ

    更新日期：1995-08-01 00:00:00

  • Comparative analyses of Podospora anserina secretomes reveal a large array of lignocellulose-active enzymes.

    abstract：:The genome of the coprophilous fungus Podospora anserina harbors a large and highly diverse set of putative lignocellulose-acting enzymes. In this study, we investigated the enzymatic diversity of a broad range of P. anserina secretomes induced by various carbon sources (dextrin, glucose, xylose, arabinose, lactose, c...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-5698-3

    authors： Poidevin L,Berrin JG,Bennati-Granier C,Levasseur A,Herpoël-Gimbert I,Chevret D,Coutinho PM,Henrissat B,Heiss-Blanquet S,Record E

    更新日期：2014-09-01 00:00:00