Insertion of transposon in the vicinity of SSK2 confers enhanced tolerance to furfural in Saccharomyces cerevisiae.

abstract：:Thraustochytrium sp. 26185, a unicellular marine protist, synthesizes docosahexaenoic acid, an omega-3 very long chain polyunsaturated fatty acid (VLC-PUFAs), by a polyunsaturated fatty acid (PUFA) synthase comprising three large subunits with multiple catalytic dehydratase (DH) domains critical for introducing double...

journal_title：Applied microbiology and biotechnology

authors： Xie X,Meesapyodsuk D,Qiu X

更新日期：2018-01-01 00:00:00

abstract：:Cryparin is an abundant cell-wall-associated hydrophobin of Cryphonectria parasitica. Although cryparin is encoded as a single copy gene, it is the most abundant protein produced by this fungus when grown in liquid culture. Studies to characterize the transcriptional regulatory element(s) found that the fragment betwe...

journal_title：Applied microbiology and biotechnology

authors： Kwon BR,Kim MJ,Park JA,Chung HJ,Kim JM,Park SM,Yun SH,Yang MS,Kim DH

更新日期：2009-05-01 00:00:00

abstract：:Despite airborne microorganisms representing a relevant fraction of atmospheric suspended particles, only a small amount of information is currently available on their abundance and diversity and very few studies have investigated the environmental factors influencing the structure of airborne bacterial communities. I...

journal_title：Applied microbiology and biotechnology

authors： Bertolini V,Gandolfi I,Ambrosini R,Bestetti G,Innocente E,Rampazzo G,Franzetti A

更新日期：2013-07-01 00:00:00

abstract：:Autohydrogenotrophic batch growth of Ralstonia eutropha H16 was studied in a stirred-tank reactor with nitrate and nitrite as terminal electron acceptors and the sole limiting substrates. Assuming product inhibition by nitrite, saturation kinetics with the two limiting substrates and a simple switching function, which...

journal_title：Applied microbiology and biotechnology

authors： Tiemeyer A,Link H,Weuster-Botz D

更新日期：2007-08-01 00:00:00

abstract：:γ-Aminobutyric acid (GABA) has been reported to play a neurotransmitter in the central nervous system thereby exerting an inhibition in nerve impulse, in turn ameliorating depression; in addition, recent study also reveals the anti-hypertensive effect of GABA in vivo. Edible fungi of the Monascus species have been use...

journal_title：Applied microbiology and biotechnology

authors： Lee BH,Pan TM

更新日期：2012-06-01 00:00:00

abstract：:Extremely acidophilic microorganisms have an optimal pH of <3 and are found in all three domains of life. As metals are more soluble at acid pH, acidophiles are often challenged by very high metal concentrations. Acidophiles are metal-tolerant by both intrinsic, passive mechanisms as well as active systems. Passive me...

journal_title：Applied microbiology and biotechnology

authors： Dopson M,Holmes DS

更新日期：2014-10-01 00:00:00

abstract：:With a cell concentration of 125 g dry biomass l-1 and a dilution rate of 0.1 h-1, Propionibacterium acidipropionici produces 30 g propionic acid l-1 from sugar with a productivity of 3 g l-1 h-1. The yield of propionic acid is approx. 0.36-0.45 g propionic acid g-1 sucrose and is independent of the dilution rate and ...

journal_title：Applied microbiology and biotechnology

authors： Quesada-Chanto A,Afschar AS,Wagner F

更新日期：1994-06-01 00:00:00

abstract：:Physico-chemical parameters, hydrological conditions, and microbial interactions can affect the growth and persistence of cyanobacteria, but the interacting effects among these bloom-forming factors are still poorly known. This hampers our capacity to predict the occurrence of cyanobacterial bloom accurately. Here, we...

journal_title：Applied microbiology and biotechnology

authors： Song H,Xu J,Lavoie M,Fan X,Liu G,Sun L,Fu Z,Qian H

更新日期：2017-02-01 00:00:00

abstract：:The first two genes of the threonine pathway, ask and asd, were cloned and sequenced from the aminoethoxyvinylglycine-producing Streptomyces sp. NRRL 5331. The two genes are organized in a bicistronic operon. ask, encoding the apartokinase (ASK), is located upstream from asd. The presence of a ribosome-binding site wi...

journal_title：Applied microbiology and biotechnology

authors： Cuadrado Y,Fernández M,Recio E,Aparicio JF,Martín JF

更新日期：2004-04-01 00:00:00

abstract：:Escherichia coli cells expressing mink (Mustela vison) growth hormone were grown in a batch fermentation process. The expression level was estimated to be 27% of the total cellular protein after 3 h of induction with 1 mM isopropyl beta-D-thiogalactoside (IPTG). If the expression of mink growth hormone (mGH) was induc...

journal_title：Applied microbiology and biotechnology

authors： Sereikaite J,Statkute A,Morkunas M,Radzevicius K,Borromeo V,Secchi C,Bumelis VA

更新日期：2007-02-01 00:00:00

abstract：:In this study, an evolved Saccharomyces cerevisiae HJ7-14 with high ability of algae-based ethanol production was characterized by multi-omic approaches. Genome sequencing of the HJ7-14 revealed a point mutation in the GAL83 gene (G703A) involved in the catabolite repression as well as the galactose metabolism. Cultur...

journal_title：Applied microbiology and biotechnology

authors： Kim SJ,Lee JE,Lee DY,Park H,Kim KH,Park YC

更新日期：2018-10-01 00:00:00

abstract：:Dengue viruses are emerging mosquito-borne pathogens belonging to Flaviviridae family which are transmitted to humans via the bites of infected mosquitoes Aedes aegypti and Aedes albopictus. Because of the wide distribution of these mosquito vectors, more than 2.5 billion people are approximately at risk of dengue inf...

journal_title：Applied microbiology and biotechnology

authors： Fahimi H,Mohammadipour M,Haddad Kashani H,Parvini F,Sadeghizadeh M

更新日期：2018-04-01 00:00:00

abstract：:This study examines the bioremediation potential and cadmium-induced cellular response on a molecular level in Candida tropicalis 3Aer. Spectroscopic analysis clearly illustrated the involvement of yeast cell wall components in biosorption. Cadmium bioaccumulation was confirmed by TEM, SEM, and EDX examination. TEM im...

journal_title：Applied microbiology and biotechnology

authors： Khan Z,Rehman A,Nisar MA,Zafar S,Hussain SZ,Zerr I,Hussain I,Waseem M,Arif M

更新日期：2017-10-01 00:00:00

abstract：:A widespread and hitherto by far underexploited potential among ecologically diverse fungi to pretreat wheat straw and digestate from maize silage in the future perspective of using such lignocellulosic feedstock for fermentative bioenergy production was inferred from a screening of nine freshwater ascomycetes, 76 iso...

journal_title：Applied microbiology and biotechnology

authors： Singh S,Harms H,Schlosser D

更新日期：2014-04-01 00:00:00

abstract：:Pyranose 2-oxidase (P2O) was purified 43-fold to apparent homogeneity from the basidiomycete Phanerochaete chrysosporium using liquid chromatography on phenyl Sepharose, Mono Q (twice) and phenyl Superose. The native enzyme has a molecular mass of about 250 kDa (based on native PAGE) and is composed of four identical ...

journal_title：Applied microbiology and biotechnology

authors： Artolozaga MJ,Kubátová E,Volc J,Kalisz HM

更新日期：1997-05-01 00:00:00

abstract：:Suspension cultures of the endemic South-African plant Cyclopia subternata were established for the first time and evaluated for the presence of isoflavones. The influence of light, as well as medium supplementation strategies with phenylalanine, casein hydrolysate and coconut water on biomass growth and isoflavone pr...

journal_title：Applied microbiology and biotechnology

authors： Kokotkiewicz A,Luczkiewicz M,Kowalski W,Badura A,Piekus N,Bucinski A

更新日期：2013-10-01 00:00:00

abstract：:The TM1072 gene from Thermotoga maritima codifies for a putative form of a rhamnulose-1-phosphate aldolase (Rha-1PA Tm). To investigate this enzyme further, its gene was cloned and expressed in Escherichia coli. The purified enzyme was activated by Co(2+) as a divalent metal ion cofactor, instead of Zn(2+) as its E. c...

journal_title：Applied microbiology and biotechnology

authors： Oroz-Guinea I,Sánchez-Moreno I,Mena M,García-Junceda E

更新日期：2015-04-01 00:00:00

abstract：:Alcoholic fermentation of grape must is a complex process, involving several yeast genera and species. The early stages in fermentation are dominated by non-Saccharomyces yeasts that are gradually replaced by the Saccharomyces cerevisiae species, which takes over the fermentation. Quantitative studies have reported th...

journal_title：Applied microbiology and biotechnology

authors： Renault PE,Albertin W,Bely M

更新日期：2013-05-01 00:00:00

abstract：:Mitochondrial dysfunction in Saccharomyces cerevisiae was selected as a marker of ion penetration following carbon ion beam (CIB) irradiation. Respiration-deficient mutants were screened. Following confirmation of negligible spontaneous mutation, eight genetically stable S. cerevisiae respiration-deficient mutant stra...

journal_title：Applied microbiology and biotechnology

authors： Guo X,Zhang M,Gao Y,Cao G,Yang Y,Lu D,Li W

更新日期：2019-02-01 00:00:00

abstract：:Enzymatic kinetic resolution is a widely used biotechnological tool for the production of enantiomerically pure/enriched compounds. This technique takes advantage of the enantioselectivity or enantiospecificity of an enzyme for one of the enantiomers of a racemic substrate to isolate the desired isomer. N-Carbamoyl-D-...

journal_title：Applied microbiology and biotechnology

authors： Martínez-Rodríguez S,Martínez-Gómez AI,Rodríguez-Vico F,Clemente-Jiménez JM,Las Heras-Vázquez FJ

更新日期：2010-01-01 00:00:00

abstract：:The engineered Saccharomyces cerevisiae strain △faa1△faa4 [Acot5s] was demonstrated to accumulate more free fatty acids (FFA) previously. Here, comparative proteomic analysis was performed to get a global overview of metabolic regulation in the strain. Over 500 proteins were identified, and 82 of those proteins were f...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Lee JJL,Zhang J,Chen WN

更新日期：2016-02-01 00:00:00

abstract：:In this study, we used the non-carotenogenic yeast Pichia pastoris X33 as a receptor for β-carotene-encoding genes, in order to obtain new recombinant strains capable of producing different carotenoidic compounds. We designed and constructed two plasmids, pGAPZA-EBI* and pGAPZA-EBI*L*, containing the genes encoding ly...

journal_title：Applied microbiology and biotechnology

authors： Araya-Garay JM,Feijoo-Siota L,Rosa-dos-Santos F,Veiga-Crespo P,Villa TG

更新日期：2012-03-01 00:00:00

abstract：:Escherichia coli-the powerhouse for recombinant protein production-is rapidly gaining status as a reliable and efficient host for secretory expression. An improved understanding of protein translocation processes and its mechanisms has inspired and accelerated the development of new tools and applications in this fiel...

journal_title：Applied microbiology and biotechnology

authors： Low KO,Muhammad Mahadi N,Md Illias R

更新日期：2013-05-01 00:00:00

abstract：:The human granulocyte macrophage colony-stimulating factor (GM-CSF) is a glycoprotein with important clinical applications for the treatment of neutropenia and aplastic anemia and reducing infections associated with bone marrow transplants. We evaluated the potential for using a potato virus X (PVX) viral vector syste...

journal_title：Applied microbiology and biotechnology

authors： Zhou F,Wang ML,Albert HH,Moore PH,Zhu YJ

更新日期：2006-10-01 00:00:00

abstract：:This paper reports the in vivo expression of the synthetic transposase gene tnp(a) from a hyperactive Tn5 tnp gene mutant in Streptomyces coelicolor. Using the synthetic tnp(a) gene adapted for Streptomyces codon usage, we showed random insertion of the transposon into the Streptomycetes genome. The insertion frequenc...

journal_title：Applied microbiology and biotechnology

authors： Petzke L,Luzhetskyy A

更新日期：2009-07-01 00:00:00

abstract：:Fidaxomicin, an 18-membered macrolide antibiotic, is highly active against Clostridium difficile, the most common cause of diarrhea in hospitalized patients. Though the biosynthetic mechanism of fidaxomicin has been well studied, little is known about its regulatory mechanism. Here, we reported that FadR1, a LAL famil...

journal_title：Applied microbiology and biotechnology

authors： Li YP,Yu P,Li JF,Tang YL,Bu QT,Mao XM,Li YQ

更新日期：2019-09-01 00:00:00

abstract：:Drug resistance occurrence is a global healthcare concern responsible for the increased morbidity and mortality in hospitals, time of hospitalisation and huge financial loss. The failure of the most antibiotics to kill "superbugs" poses the urgent need to develop innovative strategies aimed at not only controlling bac...

journal_title：Applied microbiology and biotechnology

authors： Bassegoda A,Ivanova K,Ramon E,Tzanov T

更新日期：2018-03-01 00:00:00

abstract：:Bacillus cereus 905, one of the plant growth-promoting rhizobacteria (PGPRs), is capable of colonizing wheat roots in a large population size. From previous studies, we learned that the sodA2-encoding manganese-containing superoxide dismutase (MnSOD2) is important for B. cereus 905 to survive in wheat rhizosphere. In ...

journal_title：Applied microbiology and biotechnology

authors： Gao T,Ding M,Wang Q

更新日期：2020-11-01 00:00:00

abstract：:Biofilm development in methanogenic fluidized-bed reactors with sand as the carrier was studied on a laboratory scale. The microorganisms present in consecutive layers of the biofilm of mature sludge granules were preliminarily characterized on the basis of their morphology, element composition and adhesion capacity a...

journal_title：Applied microbiology and biotechnology

authors： Lauwers AM,Heinen W,Gorris LG,van der Drift C

更新日期：1990-06-01 00:00:00

abstract：:The nutritional medium requirement for biomass and triterpenoid production by Antrodia cinnamomea AC0623 strain was optimized. Box-Behnken was applied to optimize biomass and triterpenoid production. According to response surface methodology (RSM), the optimum concentrations of N-source were determined. The results in...

journal_title：Applied microbiology and biotechnology

authors： Chang CY,Lee CL,Pan TM

更新日期：2006-10-01 00:00:00