An innovative tool reveals interaction mechanisms among yeast populations under oenological conditions.

abstract：:Clostridium tyrobutyricum is a promising organism for butyrate and n-butanol production, but cannot grow on sucrose. Three genes (scrA, scrB, and scrK) involved in the sucrose catabolic pathway, along with an aldehyde/alcohol dehydrogenase gene, were cloned from Clostridium acetobutylicum and introduced into C. tyrobu...

journal_title：Applied microbiology and biotechnology

authors： Zhang J,Yu L,Xu M,Yang ST,Yan Q,Lin M,Tang IC

更新日期：2017-05-01 00:00:00

abstract：:2-Oxoglutarate (2OG) is a metabolite from the highly conserved Krebs cycle and not only plays a critical role in metabolism but also acts as a signaling molecule in a variety of organisms. Environmental inorganic nitrogen is reduced to ammonium by microorganisms, whose metabolic pathways involve the conversion of 2OG ...

journal_title：Applied microbiology and biotechnology

authors： Zhang C,Wei ZH,Ye BC

更新日期：2013-09-01 00:00:00

abstract：:Biotin-dependent human acetyl-CoA carboxylases (ACCs) are integral in homeostatic lipid metabolism. By securing posttranslational biotinylation, ACCs perform coordinated catalytic functions allosterically regulated by phosphorylation/dephosphorylation and citrate. The production of authentic recombinant ACCs is heeded...

journal_title：Applied microbiology and biotechnology

authors： Hwang IW,Makishima Y,Kato T,Park S,Terzic A,Park EY

更新日期：2014-10-01 00:00:00

abstract：:1,3-1,4-beta-D-glucanase is an important endoglycosidase in the brewing and animal feed industries. To achieve high-level expression of recombinant glucanase in Pichia pastoris, we designed sequences encoding the alpha-factor signal peptide from Saccharomyces cerevisiae and the truncated 1,3-1,4-beta-D-glucanase from ...

journal_title：Applied microbiology and biotechnology

authors： Huang H,Yang P,Luo H,Tang H,Shao N,Yuan T,Wang Y,Bai Y,Yao B

更新日期：2008-02-01 00:00:00

abstract：:Wild-type Corynebacterium glutamicum produced 0.6 g l(-1) xylitol from xylose at a productivity of 0.01 g l(-1) h(-1) under oxygen deprivation. To increase this productivity, the pentose transporter gene (araE) from C. glutamicum ATCC31831 was integrated into the C. glutamicum R chromosome. Consequent disruption of it...

journal_title：Applied microbiology and biotechnology

authors： Sasaki M,Jojima T,Inui M,Yukawa H

更新日期：2010-04-01 00:00:00

abstract：:The substrate selectivity of numerous commercially available lipases from microorganisms, plants and animal tissue towards 9-octadecenoic acids with respect to the cis/trans configuration of the C=C double bond was examined by the esterification of cis- and trans-9-octadecanoic acid (oleic and elaidic acid respectivel...

journal_title：Applied microbiology and biotechnology

authors： Borgdorf R,Warwel S

更新日期：1999-04-01 00:00:00

abstract：:Immunostimulatory glucose polymers known as beta-glucans have been studied for many years. Our laboratory has prepared and characterized a novel microparticulate beta-glucan (MG) from the budding yeast Saccharomyces cerevisiae. Because MG particles are rapidly phagocytized by murine peritoneal macrophages and induce t...

journal_title：Applied microbiology and biotechnology

authors： Berner VK,Sura ME,Hunter KW Jr

更新日期：2008-10-01 00:00:00

abstract：:The human intestine hosts various complex microbial communities that are closely associated with multiple health and disease processes. Determining the composition and function of these microbial communities is critical to unveil disease mechanisms and promote human health. Recently, meta-omic strategies have been dev...

journal_title：Applied microbiology and biotechnology

authors： Xiao M,Yang J,Feng Y,Zhu Y,Chai X,Wang Y

更新日期：2017-04-01 00:00:00

abstract：:In this study, the kinetic behaviors between n-butyl acetate and composite bead were investigated. Both microbial growth rate and biochemical reaction rate would be inhibited with increasing average inlet concentration. The order of the inhibitive effect, which resulted from increased average inlet concentration for f...

journal_title：Applied microbiology and biotechnology

authors： Chan WC,Hwang ID

更新日期：2006-11-01 00:00:00

abstract：:There is a worldwide trend to understand the impact of non-Saccharomyces yeast species on the process of winemaking. Although the predominant species at the end of the fermentation is Saccharomyces cerevisiae, several non-Saccharomyces species present during the first days of the process can produce and/or release aro...

journal_title：Applied microbiology and biotechnology

authors： Barquet M,Martín V,Medina K,Pérez G,Carrau F,Gaggero C

更新日期：2012-01-01 00:00:00

abstract：:Hepatic lipid metabolic disorders and oxidative stress are involved in the development of non-alcoholic fatty liver disease (NAFLD). This study is to determine the protective effects of Lactobacillus plantarum NA136 on high-fat diet and fructose (HFD/F)-induced NAFLD and to elucidate its underlying molecular mechanism...

journal_title：Applied microbiology and biotechnology

authors： Zhao Z,Wang C,Zhang L,Zhao Y,Duan C,Zhang X,Gao L,Li S

更新日期：2019-07-01 00:00:00

abstract：:Anaerobic biodegradation of atrazine by the bacterial isolate M91-3 was characterized with respect to mineralization, metabolite formation, and denitrification. The ability of the isolate to enhance atrazine biodegradation in anaerobic sediment slurries was also investigated. The organism utilized atrazine as its sole...

journal_title：Applied microbiology and biotechnology

authors： Crawford JJ,Sims GK,Mulvaney RL,Radosevich M

更新日期：1998-05-01 00:00:00

abstract：:Biocatalytic asymmetric preparation of (R)-1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent (R)-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on m...

journal_title：Applied microbiology and biotechnology

authors： Zheng RC,Ge Z,Qiu ZK,Wang YS,Zheng YG

更新日期：2012-05-01 00:00:00

abstract：:Clavulanic acid is a secondary metabolite produced by Streptomyces clavuligerus. It possesses a clavam structure and a characteristic 3R,5R stereochemistry essential for action as a beta-lactamase inhibitory molecule. It is produced from glyceraldehyde-3-phosphate and arginine in an eight step biosynthetic pathway. Th...

journal_title：Applied microbiology and biotechnology

authors： Liras P,Rodríguez-García A

更新日期：2000-10-01 00:00:00

abstract：:Biosynthesis has emerged as a frontier technology for fabrication of functionally diverse nanoparticles that possess tremendous therapeutic implications. Various biological resources have already demonstrated their potential to produce nanoparticles with interesting features. Endophytic microbes live in a symbiotic re...

journal_title：Applied microbiology and biotechnology

authors： Rahman S,Rahman L,Khalil AT,Ali N,Zia D,Ali M,Shinwari ZK

更新日期：2019-03-01 00:00:00

abstract：:This study is an application of an experimental design methodology for the optimization of the culture conditions of alpha-L: -arabinofuranosidase production by Talaromyces thermophilus. Wheat bran and yeast extract were first selected as the best carbon and nitrogen sources, respectively, for enzyme production. A Pla...

journal_title：Applied microbiology and biotechnology

authors： Guerfali M,Chaabouni M,Gargouri A,Belghith H

更新日期：2010-02-01 00:00:00

abstract：:Formate dehydrogenases (FDH) are useful for the regeneration of NADH, which is required for asymmetric reduction by several dehydrogenases and reductases. FDHs have relatively low activity and are labile, especially to alpha-haloketones, thus FDH cannot be applied to the industrial manufacture of optically active alph...

journal_title：Applied microbiology and biotechnology

authors： Yamamoto H,Mitsuhashi K,Kimoto N,Kobayashi Y,Esaki N

更新日期：2005-04-01 00:00:00

abstract：:The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 degrees C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability de...

journal_title：Applied microbiology and biotechnology

authors： Beney L,Marechal PA,Gervais P

更新日期：2001-08-01 00:00:00

abstract：:To optimize the expression of cry genes in a Bacillus thuringiensis sigK mutant failing in crystal releasing, the transcriptional activity of the cry promoters cry1A, cry3A, cry4A, and cry8E was compared using lacZ gene fusions. A beta-galactosidase assay indicated that the cry8E promoter showed the highest transcript...

journal_title：Applied microbiology and biotechnology

authors： Zhou C,Zheng Q,Peng Q,Du L,Shu C,Zhang J,Song F

更新日期：2014-09-01 00:00:00

abstract：:Inteins are internal protein elements that self-excise from their host protein and catalyze ligation of the flanking sequences (exteins) with a peptide bond. They are found in organisms in all three domains of life, and in viral proteins. Intein excision is a posttranslational process that does not require auxiliary e...

journal_title：Applied microbiology and biotechnology

authors： Elleuche S,Pöggeler S

更新日期：2010-06-01 00:00:00

abstract：:To improve the stability and catalytic efficiency of pyranose 2-oxidase (P2Ox) by molecular enzyme evolution, we cloned P2Ox cDNA by RACE-PCR from a cDNA library derived from the basidiomycete Peniophora gigantea. The P2Ox gene was expressed in Escherichia coli BL21(DE3), yielding an intracellular and enzymatically ac...

journal_title：Applied microbiology and biotechnology

authors： Bastian S,Rekowski MJ,Witte K,Heckmann-Pohl DM,Giffhorn F

更新日期：2005-06-01 00:00:00

abstract：:Conductive materials can facilitate syntrophic methane (CH4) production by improving direct interspecies electron transfer. The effect of a conductive material, ferroferric oxide (Fe3O4), on the anaerobic treatment of tryptone-based high-strength wastewater was investigated in two anaerobic sequencing batch reactors (...

journal_title：Applied microbiology and biotechnology

authors： Yin Q,He K,Liu A,Wu G

更新日期：2017-05-01 00:00:00

abstract：:A single-stage continuous fermentation process for the production of 2-keto-L-gulonic acid (2KGA) from L-sorbose using Ketogulonigenium vulgare DSM 4025 was developed. The chemostat culture with the dilution rate that was calculated based on the relationship between the 2KGA production rate and the 2KGA concentration ...

journal_title：Applied microbiology and biotechnology

authors： Takagi Y,Sugisawa T,Hoshino T

更新日期：2009-04-01 00:00:00

abstract：:A new tool to provide an environmentally friendly way to deliver active proteins to the environment has been developed, based on the use of polyhydroxyalkanoate (PHA, bioplastic) granules. To illustrate this novel approach, a derived Cry1Ab insect-specific toxin protein was in vivo immobilized into PHA granules throug...

journal_title：Applied microbiology and biotechnology

authors： Moldes C,Farinós GP,de Eugenio LI,García P,García JL,Ortego F,Hernández-Crespo P,Castañera P,Prieto MA

更新日期：2006-08-01 00:00:00

abstract：:Bacillus thuringiensis bacteria show insecticidal activities that rely upon the production of insecticidal crystal proteins, which are encoded by cry or cyt genes and can target a variety of insect pests. It has been shown that cry1Ac is the only cry gene in B. thuringiensis subsp. kurstaki HD73 (B. thuringiensis HD73...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Gao T,Peng Q,Song L,Zhang J,Chai Y,Sun D,Song F

更新日期：2018-04-01 00:00:00

abstract：:In insects, glutathione S-transferases (GSTs) are enzymes involved in detoxification of insecticides. However, few data are available for the codling moth, Cydia pomonella (L.). In this study, we cloned a delta class GST gene CpGSTd1 from C. pomonella. Real-time quantitative PCR shows that CpGSTd1 was up-regulated wit...

journal_title：Applied microbiology and biotechnology

authors： Liu J,Yang X,Zhang Y

更新日期：2014-11-01 00:00:00

abstract：:The kinetics of growth, acid and solvent production in batch culture of Clostridium pasteurianum DSMZ 525 were examined in mixed or mono-substrate fermentations. In pH-uncontrolled batch cultures, the addition of butyric acid or glucose significantly enhanced n-butanol production and the ratio of butanol/1,3-propanedi...

journal_title：Applied microbiology and biotechnology

authors： Sabra W,Groeger C,Sharma PN,Zeng AP

更新日期：2014-05-01 00:00:00

abstract：:D-Danshensu (D-DSS), a traditional Chinese medicine, is used to treat cardiovascular and cerebrovascular diseases. However, current isolation protocols for D-DSS both natural and synthetic are not ideal; therefore, in this study, we have developed a whole-cell biotransformation method to produce D-DSS from L-DOPA. Thi...

journal_title：Applied microbiology and biotechnology

authors： Xiong T,Jiang J,Bai Y,Fan TP,Zhao Y,Zheng X,Cai Y

更新日期：2019-08-01 00:00:00

abstract：:Pneumocandin B0, the precursor of the antifungal drug caspofungin, is a secondary metabolite of the fungus Glarea lozoyensis. In this study, we investigated the effects of mannitol as the sole carbon source on pneumocandin B0 production by G. lozoyensis. The osmotic pressure is more important in enhancing pneumocandin...

journal_title：Applied microbiology and biotechnology

authors： Song P,Huang B,Zhang S,Zhang K,Yuan K,Ji X,Ren L,Wen J,Huang H

更新日期：2018-12-01 00:00:00

abstract：:Sulfur-containing aroma compounds are key contributors to the flavour of a diverse range of foods and beverages, such as wine. The tropical fruit characters of Sauvignon Blanc wines are attributed to the presence of the aromatic thiols 3-mercaptohexan-1-ol (3-MH), its acetate ester 3-mercaptohexyl acetate (3-MHA), and...

journal_title：Applied microbiology and biotechnology

authors： Cordente AG,Capone DL,Curtin CD

更新日期：2015-11-01 00:00:00