Metaproteomic strategies and applications for gut microbial research.

abstract：:Aldolases are a specific group of lyases that catalyze the reversible stereoselective addition of a donor compound (nucleophile) onto an acceptor compound (electrophile). Whereas most aldolases are specific for their donor compound in the aldolization reaction, they often tolerate a wide range of aldehydes as acceptor...

journal_title：Applied microbiology and biotechnology

authors： Samland AK,Sprenger GA

更新日期：2006-07-01 00:00:00

abstract：:Rhodococcus erythropolis N9T-4, which is an extremely oligotrophic bacterium, can survive in a completely inorganic medium with no additional carbon source. This bacterium utilizes atmospheric CO2, but does not require any additional energy source such as light and hydrogen gas, required by autotrophic microorganisms....

journal_title：Applied microbiology and biotechnology

authors： Matsuoka T,Yoshida N

更新日期：2019-05-01 00:00:00

abstract：:Clavulanic acid is a secondary metabolite produced by Streptomyces clavuligerus. It possesses a clavam structure and a characteristic 3R,5R stereochemistry essential for action as a beta-lactamase inhibitory molecule. It is produced from glyceraldehyde-3-phosphate and arginine in an eight step biosynthetic pathway. Th...

journal_title：Applied microbiology and biotechnology

authors： Liras P,Rodríguez-García A

更新日期：2000-10-01 00:00:00

abstract：:Cultivation processes involving filamentous fungi have been optimised for decades to obtain high product yields. Several bulk chemicals like citric acid and penicillin are produced this way. A simple adaptation of cultivation parameters for new production processes is not possible though. Models explaining the correla...

journal_title：Applied microbiology and biotechnology

authors： Grimm LH,Kelly S,Krull R,Hempel DC

更新日期：2005-12-01 00:00:00

abstract：:In this study, we employed TiO₂ enrichment and high accuracy liquid chromatography-mass spectrometry-mass spectrometry to identify the phosphoproteome of Clostridium acetobutyicum ATCC824 in acidogenesis and solventogenesis. As many as 82 phosphopeptides in 61 proteins, with 107 phosphorylated sites on serine, threoni...

journal_title：Applied microbiology and biotechnology

authors： Bai X,Ji Z

更新日期：2012-07-01 00:00:00

abstract：:Exophiala jeanselmei is an opportunistic pathogenic black yeast growing in humid environments such as water reservoirs of air-conditioning systems. Because this fungal contaminant could be vaporized into the air and subsequently cause health problems, its monitoring is recommended. Currently, this monitoring is based ...

journal_title：Applied microbiology and biotechnology

authors： Libert X,Chasseur C,Packeu A,Bureau F,Roosens NH,De Keersmaecker SJC

更新日期：2016-02-01 00:00:00

abstract：:In this study, we applied a 16S ribosomal RNA (rRNA) metagenomics approach to survey inanimate hospital environments (IHEs) in a respiratory care center (RCC). A total of 16 samples, including 9 from medical devices and 7 from workstations, were analyzed. Besides, clinical isolates were retrospectively analyzed during...

journal_title：Applied microbiology and biotechnology

authors： Tang CY,Yiu SM,Kuo HY,Tan TS,Liao KH,Liu CC,Hon WK,Liou ML

更新日期：2015-03-01 00:00:00

abstract：:A simple and stable immobilization of a laccase from Pleurotus ostreatus was obtained through genetic fusion with a self-assembling and adhesive class I hydrophobin. The chimera protein was expressed in Pichia pastoris and secreted into the culture medium. The crude culture supernatant was directly used for coatings o...

journal_title：Applied microbiology and biotechnology

authors： Sorrentino I,Giardina P,Piscitelli A

更新日期：2019-04-01 00:00:00

abstract：:UV-mediated mutagenesis generated a high glucoamylase-producing mutant of Aspergillus oryzae exhibiting strong melanization in solid-state culture. Expression of the glucoamylase-encoding gene (glaB), which is specifically expressed in solid-state culture, and the tyrosinase-encoding gene (melO), was analyzed using an...

journal_title：Applied microbiology and biotechnology

authors： Ishida H,Matsumura K,Hata Y,Kawato A,Suginami K,Abe Y,Imayasu S,Ichishima E

更新日期：2001-10-01 00:00:00

abstract：:A batch fermentation strategy using Xanthomonas campestris ATCC 13951 for xanthan gum production has been established in which all essential medium components are supplied at the onset. This has been achieved using sucrose as sole sugar feedstock. Sequential consumption of nitrogen sources (soybean hydrolysates, ammon...

journal_title：Applied microbiology and biotechnology

authors： Letisse F,Chevallereau P,Simon JL,Lindley ND

更新日期：2001-05-01 00:00:00

abstract：:Although several bacterial lignin-oxidising enzymes have been discovered in recent years, it is not yet clear whether different lignin-degrading bacteria use similar mechanisms for lignin oxidation and degradation of lignin fragments. Genome sequences of 13 bacterial lignin-oxidising bacteria, including new genome seq...

journal_title：Applied microbiology and biotechnology

authors： Granja-Travez RS,Persinoti GF,Squina FM,Bugg TDH

更新日期：2020-04-01 00:00:00

abstract：:Using organically synthesized hydroxyalkanoate coenzyme A thioesters, the activities of two short-chain polyhydroxalkanoate (PHA) synthases were investigated--Ralstonia eutropha PHA synthase (a type I PHA synthase) and Ectothiorhodospira shaposhnikovii PHA synthase (a type III synthase). The results indicate that the ...

journal_title：Applied microbiology and biotechnology

authors： Zhang S,Kamachi M,Takagi Y,Lenz RW,Goodwin S

更新日期：2001-07-01 00:00:00

abstract：:Chlorogenate hydrolase (EC 3.1.1.42, CHase) was highly induced in mycelia of Aspergillus sojae AKU 3312 grown in Czapek medium containing either instant coffee powder or coffee pulp as inducer. No CHase formation was observed in the mycelia when cultivated without the inducer. CHase was purified readily from CHase-ind...

journal_title：Applied microbiology and biotechnology

authors： Adachi O,Ano Y,Akakabe Y,Shinagawa E,Matsushita K

更新日期：2008-11-01 00:00:00

abstract：:Xylanase is the enzyme complex that is responsible for the degradation of xylan; however, novel xylanase producers remain to be explored in marine environment. In this study, a Streptomyces strain M11 which exhibited xylanase activity was isolated from marine sediment. The 16S rDNA sequence of M11 showed the highest i...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Zhao X,Bai F

更新日期：2013-05-01 00:00:00

abstract：:The merC gene from the Tn21-encoded mer operon has potential uses as a molecular tool for bioremediation. It was overexpressed as the fusion proteins MerC-Sso1p or MerC-Vam3p in Saccharomyces cerevisiae. Green fluorescent protein (GFP)-MerC-Sso1p fusion proteins located primarily in the plasma membrane, although some ...

journal_title：Applied microbiology and biotechnology

authors： Kiyono M,Miyahara K,Sone Y,Pan-Hou H,Uraguchi S,Nakamura R,Sakabe K

更新日期：2010-03-01 00:00:00

abstract：:Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A s...

journal_title：Applied microbiology and biotechnology

authors： Morikawa M,Daido H,Pongpobpibool S,Imanaka T

更新日期：1994-11-01 00:00:00

abstract：:Biogranulation is a promising biotechnology developed for wastewater treatment. Biogranules exhibit a matrix microbial structure, and intensive research has shown that extracellular polymeric substances (EPS) are a major component of the biogranule matrix material in both anaerobic and aerobic granules. This paper aim...

journal_title：Applied microbiology and biotechnology

authors： Liu YQ,Liu Y,Tay JH

更新日期：2004-08-01 00:00:00

abstract：:MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD2 β-glucosidase activity increased in a cellobiose concen...

journal_title：Applied microbiology and biotechnology

authors： Matsuzawa T,Yaoi K

更新日期：2017-01-01 00:00:00

abstract：:The use of crude aqueous extracts of Cynara cardunculus flowers as coagulants in the production of high-quality sheep and goat cheeses-as are the cases of several Portuguese and Spanish cheese varieties with Protected Designation of Origin status-has been maintained since ancient times. The unique rheological attribut...

journal_title：Applied microbiology and biotechnology

authors： Almeida CM,Simões I

更新日期：2018-06-01 00:00:00

abstract：:Uniform stable-isotope labeling of mammalian cells is achieved via a novel formulation of a serum-free cell culture medium that is based on stable-isotope-labeled autolysates and lipid extracts of various microbiological origin. Yeast autolysates allow complete replacement of individual amino acids and organic acids i...

journal_title：Applied microbiology and biotechnology

authors： Egorova-Zachernyuk TA,Bosman GJ,Degrip WJ

更新日期：2011-01-01 00:00:00

abstract：:Thraustochytrium sp. 26185, a unicellular marine protist, synthesizes docosahexaenoic acid, an omega-3 very long chain polyunsaturated fatty acid (VLC-PUFAs), by a polyunsaturated fatty acid (PUFA) synthase comprising three large subunits with multiple catalytic dehydratase (DH) domains critical for introducing double...

journal_title：Applied microbiology and biotechnology

authors： Xie X,Meesapyodsuk D,Qiu X

更新日期：2018-01-01 00:00:00

abstract：:Microalgal based biofuels are discussed as future sustainable energy source because of their higher photosynthetic and water use efficiency to produce biomass. In the context of climate CO2 mitigation strategies, algal mass production is discussed as a potential CO2 sequestration technology which uses CO2 emissions to...

journal_title：Applied microbiology and biotechnology

authors： Wilhelm C,Jakob T

更新日期：2011-12-01 00:00:00

abstract：:The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic ni...

journal_title：Applied microbiology and biotechnology

authors： Saha BC

更新日期：2006-10-01 00:00:00

abstract：:A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with ...

journal_title：Applied microbiology and biotechnology

authors： Wang HX,Ng TB

更新日期：2006-12-01 00:00:00

abstract：:Porcine circovirus type 3 (PCV3) is an emerging swine pathogen associated with acute porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs, reproductive failure, and multisystemic inflammation. Current evidence shows that PCV3 is spread worldwide, and its high incidence may pose a threat to the global...

journal_title：Applied microbiology and biotechnology

authors： Jiang M,Guo J,Zhang G,Jin Q,Liu Y,Jia R,Wang A

更新日期：2020-07-01 00:00:00

abstract：:Brown rot wood-degrading fungi distinctly modify lignocellulose and completely hydrolyze polysaccharides (saccharification), typically without secreting an exo-acting glucanase and without removing lignin. Although each step of this two-step approach evolved within the same organism, it is unknown if the early lignoce...

journal_title：Applied microbiology and biotechnology

authors： Schilling JS,Tewalt JP,Duncan SM

更新日期：2009-09-01 00:00:00

abstract：:The ω-3 fatty acid desaturase (ω3Des) is a key enzyme in the biosynthesis of polyunsaturated fatty acids (PUFAs). However, the enzyme exhibits a significant preference towards different fatty acid substrates. To examine the molecular mechanism of its substrate specificity, a series of site-directed mutants were constr...

journal_title：Applied microbiology and biotechnology

authors： Rong C,Chen H,Wang M,Gu Z,Zhao J,Zhang H,Chen W,Chen YQ

更新日期：2018-11-01 00:00:00

abstract：:Enzymatic kinetic resolution is a widely used biotechnological tool for the production of enantiomerically pure/enriched compounds. This technique takes advantage of the enantioselectivity or enantiospecificity of an enzyme for one of the enantiomers of a racemic substrate to isolate the desired isomer. N-Carbamoyl-D-...

journal_title：Applied microbiology and biotechnology

authors： Martínez-Rodríguez S,Martínez-Gómez AI,Rodríguez-Vico F,Clemente-Jiménez JM,Las Heras-Vázquez FJ

更新日期：2010-01-01 00:00:00

abstract：:Myo-inositol is important for Streptomyces growth and morphological differentiation. Genomic sequence analysis revealed a myo-inositol catabolic gene cluster in Streptomyces coelicolor. Disruption of the corresponding genes in this cluster abolished the bacterial growth on myo-inositol as a single carbon source. The t...

journal_title：Applied microbiology and biotechnology

authors： Yu L,Li S,Gao W,Pan Y,Tan H,Liu G

更新日期：2015-04-01 00:00:00

abstract：:We have successfully created a novel yeast strain able to monitor changes in environmental conditions by displaying either green fluorescent protein (GFP) from Aequorea victoria or blue fluorescent protein (BFP), a variant of GFP, on its cell surface as a visible reporter. For the display of these fluorescent proteins...

journal_title：Applied microbiology and biotechnology

authors： Shibasaki S,Ueda M,Ye K,Shimizu K,Kamasawa N,Osumi M,Tanaka A

更新日期：2001-11-01 00:00:00