当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Screening, identification, and characterization of a novel saccharide-stimulated β-glycosidase from a soil metagenomic library.

Screening, identification, and characterization of a novel saccharide-stimulated β-glycosidase from a soil metagenomic library.

Abstract：

:MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD2 β-glucosidase activity increased in a cellobiose concentration-dependent manner and was not inhibited by a high concentration of D-glucose or cellobiose. MeBglD2 β-glucosidase and β-fucosidase activities were activated by various monosaccharides and disaccharides including D-glucose, D-xylose, D-galactose, maltose, and cellobiose. The saccharification yield of rice straw using Trichoderma reesei cellulase was improved by the addition of MeBglD2. These results show that MeBglD2 can be used to improve plant biomass saccharification, because both substrates and products can activate its enzymatic activity.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Matsuzawa T,Yaoi K

doi

10.1007/s00253-016-7803-2

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

633-646

issue

2

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-016-7803-2

journal_volume

101

pub_type

杂志文章

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