Abstract：
:Exposure of Synechococcus leopoliensis to selenite in the light resulted in orange-colored granules associated with the cells. No such particles were made in dark grown cells or when selenite was replaced by selenate. Light and scanning electron microscopy revealed that the particles formed inside the cells. Furthermore, these were easily extracted and shown to be composed of selenium as determined by energy-dispersive X-ray spectroscopy. During selenium particle synthesis there was a concurrent loss of organic pigments in the cyanobacteria. Cells also become heavier as they produced and accumulated particles which were on average 220 nm in diameter and generally spherical in shape. The decline in selenite concentration in the culture media can be accounted for by the formation of cellular elemental selenium (Se(0)) during particle formation, although synthesis of small amounts of other Se compounds cannot be entirely discounted. Photosynthetic activity is required for the formation of Se(0), implicating the involvement of thylakoids. It is possible that an intimate association between the nascent particles and the thylakoids occurred. However, Se(0) granule formation did not occur peripherally between the thylakoid and the cytoplasmic membranes, but inside the thylakoid bands towards the center of the cells. It then appears that the particles are mobilized to the periphery and expelled from the cells, causing irreparable damage to the cell walls.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Hnain A,Brooks J,Lefebvre DDdoi
10.1007/s00253-013-5304-0subject
Has Abstractpub_date
2013-12-01 00:00:00pages
10511-9issue
24eissn
0175-7598issn
1432-0614journal_volume
97pub_type
杂志文章abstract：:Corynebacterium glutamicum CCTCC M201005 produces a novel polygalacturonic acid bioflocculant, REA-11, consisting of galacturonic acid as the main structural unit. A biosynthetic pathway of REA-11 in C. glutamicum CCTCC M201005 was proposed. Evidence for the biosynthetic pathway was provided by: (1) analyzing the resp...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-003-1365-9
更新日期：2003-12-01 00:00:00
abstract：:Engineered S. cerevisiae employing the xylose reductase pathway enables efficient xylose valorization to fuels and chemicals. However, toxicity of thermochemically pretreated biomass hydrolysate on S. cerevisiae is one of the key technical challenges to upgrade biomass-derived sugars including xylose and glucose into ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-018-9216-x
更新日期：2018-09-01 00:00:00
abstract：:Production of (R)-3-hydroxybutyric acid [(R)-3-HB] by strain Halomonas sp. KM-1 under successive aeration conditions was investigated. The first aerobic condition allowed both cell growth and intracellular storage of poly-(R)-3-hydroxybutyric acid (PHB). The second microaerobic condition, achieved by reducing the cult...
journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-012-4218-6
更新日期：2012-11-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2010-05-01 00:00:00
abstract：:The paper describes the preparation of new probiotic formulations based on chitosan-coated alginate microcapsules containing three different probiotic strains, Lactobacillus plantarum PBS067, Lactobacillus rhamnosus PBS070, and Bifidobacterium animalis subsp. lactis PBS075 taken individually and as a mixture of them. ...
journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-015-6853-1
更新日期：2015-11-01 00:00:00
abstract：:This study was aimed to investigate the effect of a single-chain fragment variable antibody of connective tissue growth factor (anti-CTGF scFv) against the differentiation of fibroblast into myofibroblast. The scFv antibody was firstly expressed in Escherichia coli cells and was then purified by affinity chromatograph...
journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-011-3755-8
更新日期：2012-03-01 00:00:00
abstract：:Flow cytometry is a rapid and quantitative method to determine bacterial viability. Although different stains can be used to establish viability, staining protocols are inconsistent and lack a general optimization approach. Very few "true" multicolor protocols, where dyes are combined in one sample, have been develope...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2016-11-01 00:00:00
abstract：:Candida albicans is the most common clinical pathogenic fungus, which is highly susceptible to immunodeficiency. Development of novel antifungal agents has become a growing trend in the treatment of Candida infections. C16-Fengycin A, a lipopeptide isolated from Bacillus amyloliquefaciens fmb60 showed significant fung...
journal_title：Applied microbiology and biotechnology
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更新日期：2019-11-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-010-2711-3
更新日期：2010-08-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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abstract：:High vanillin productivity was achieved in the batch biotransformation of ferulic acid by Streptomyces sp. strain V-1. Due to the toxicity of vanillin and the product inhibition, fed-batch biotransformation with high concentration of ferulic acid was unsuccessful. To solve this problem and improve the vanillin yield, ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-006-0735-5
更新日期：2007-03-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-006-0655-4
更新日期：2007-02-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-004-1698-z
更新日期：2005-01-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-007-1184-5
更新日期：2007-12-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-017-8414-2
更新日期：2017-09-01 00:00:00
abstract：:Ene-reductases originating from extremophiles are gaining importance in the field of biocatalysis due to higher-stability properties. The genome of the acidophilic iron-oxidizing bacterium "Ferrovum" sp. JA12 was found to harbor a thermophilic-like ene-reductase (FOYE-1). The foye-1 gene was ligated into a pET16bp exp...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7782-3
更新日期：2017-01-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：2002-07-01 00:00:00
abstract：:A novel cell surface display system in Aspergillus oryzae was established by using a chitin-binding module (CBM) from Saccharomyces cerevisiae as an anchor protein. CBM was fused to the N or C terminus of green fluorescent protein (GFP) and the fusion proteins (GFP-CBM and CBM-GFP) were expressed using A. oryzae as a ...
journal_title：Applied microbiology and biotechnology
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更新日期：2010-08-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：2013-06-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-017-8491-2
更新日期：2017-10-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2020-07-01 00:00:00
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更新日期：2014-08-01 00:00:00
abstract：:Although the de novo biosynthetic mechanism of 3-hydroxypropionic acid (3-HP) in glycerol-fermenting microorganisms is still unclear, the propanediol utilization protein (PduP) of Lactobacillus species has been suggested to be a key enzyme in this regard. To verify this hypothesis, a pduP gene from Lactobacillus reute...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-010-2887-6
更新日期：2011-02-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530000413
更新日期：2000-10-01 00:00:00
abstract：:Bacillus cereus 905, one of the plant growth-promoting rhizobacteria (PGPRs), is capable of colonizing wheat roots in a large population size. From previous studies, we learned that the sodA2-encoding manganese-containing superoxide dismutase (MnSOD2) is important for B. cereus 905 to survive in wheat rhizosphere. In ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-020-10915-2
更新日期：2020-11-01 00:00:00
abstract：:Our previous work showed that there is a limitation in the use of dihydrofolate reductase (dhfr)/methotrexate (MTX)-mediated gene amplification systems in dhfr-non-deficient HEK293 cells, as endogenous dhfr may interfere with the amplification process. In the present study, we successfully generated Epstein-Barr virus...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2021-01-01 00:00:00
abstract：:The effect of sodium chloride (NaCl) on pullulan production by batch culture of Aureobasidium pullulans CCTCC M 2012259 was investigated. NaCl at 3 g/L improved the pullulan titer by 26.7% but reduced the molecular weight of pullulan to only 46.8% of that obtained in the control without NaCl. In order to elucidate the...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-018-9292-y
更新日期：2018-10-01 00:00:00
abstract：:At present, more than 100 strains of Lentinula edodes are cultivated on a commercial scale in China. A simple, reliable, and effective method to distinguish some commercial strains of the superior type from other commercial strains is very important for the Lentinula industry. In this study, 23 commercial strains of L...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-008-1671-3
更新日期：2008-11-01 00:00:00
abstract：:The secreted proteome of Pichia pastoris X-33 was investigated in methanol-induced cultures with a goal to enhance the secretion and purification of recombinant proteins. In a fed-batch fermentation at 30 °C, more host proteins were found in greater concentrations compared to cultures grown at 25 °C. Protein samples c...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3118-5
更新日期：2011-04-01 00:00:00
