Effect of glucose on glycerol bioconversion by Lactobacillus reuteri.

abstract：:Flow cytometry is a rapid and quantitative method to determine bacterial viability. Although different stains can be used to establish viability, staining protocols are inconsistent and lack a general optimization approach. Very few "true" multicolor protocols, where dyes are combined in one sample, have been develope...

journal_title：Applied microbiology and biotechnology

authors： Buysschaert B,Byloos B,Leys N,Van Houdt R,Boon N

更新日期：2016-11-01 00:00:00

abstract：:Chitosan raises a great interest among biotechnologists due to its potential for applications in biomedical or environmental fields. Enzymatic hydrolysis of chitosan is a recognized method allowing control of its molecular size, making possible its optimization for a given application. During the industrial hydrolysis...

journal_title：Applied microbiology and biotechnology

authors： Zitouni M,Fortin M,Scheerle RK,Letzel T,Matteau D,Rodrigue S,Brzezinski R

更新日期：2013-07-01 00:00:00

abstract：:Heterorhabditis bacteriophora is used in biological control of soil-borne insect pests in horticulture and turf. Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ) are inoculated. As ...

journal_title：Applied microbiology and biotechnology

authors： Johnigk SA,Ecke F,Poehling M,Ehlers RU

更新日期：2004-06-01 00:00:00

abstract：:Omega-3 fatty acids (ω3 FAs) have attracted attention because they have various health benefits for humans. Fish oils are currently major sources of ω3 FAs, but a sustainable supply of ω3 FAs based on fish oils is problematic because of the increasing demand. In this study, the production potential of a genetically en...

journal_title：Applied microbiology and biotechnology

authors： Yoshino T,Kakunaka N,Liang Y,Ito Y,Maeda Y,Nomaguchi T,Matsunaga T,Tanaka T

更新日期：2017-09-01 00:00:00

abstract：:Vaccination has been regarded as the most effective way to reduce death and morbidity caused by infectious diseases in the livestock industry. In this study, plasma activated water (PAW) was introduced to prepare the inactivated Newcastle disease vaccine. Humoral immune response was tested by hemagglutination inhibiti...

journal_title：Applied microbiology and biotechnology

authors： Hongzhuan Z,Ying T,Xia S,Jinsong G,Zhenhua Z,Beiyu J,Yanyan C,Lulu L,Jue Z,Bing Y,Jing F

更新日期：2020-01-01 00:00:00

abstract：:A new rapid and sensitive method for the detection of antibacterial activities was based on luminescent indicator strains. Listeria innocua 8811 and Enterococcus faecalis 32 were transformed with plasmid carrying bacterial luciferase genes. Subsequent strains became capable to emit light during the exponential growth ...

journal_title：Applied microbiology and biotechnology

authors： Simon L,Fremaux C,Cenatiempo Y,Berjeaud JM

更新日期：2001-12-01 00:00:00

abstract：:Epoxide hydrolases (EHs) of fungal origin have the ability to catalyze the enantioselective hydrolysis of epoxides to their corresponding diols. However, wild type fungal EHs are limited in substrate range and enantioselectivity. Additionally, the production of fungal epoxide hydrolase (EH) by wild-type strains is typ...

journal_title：Applied microbiology and biotechnology

authors： Maharajh D,Roth R,Lalloo R,Simpson C,Mitra R,Görgens J,Ramchuran S

更新日期：2008-05-01 00:00:00

abstract：:The fungal conversions of sulfur-containing heterocyclic compounds were investigated using the lignin-degrading basidiomycete Coriolus versicolor. The fungus metabolized a series of sulfur compounds--25 structurally related thiophene derivatives--via several different pathways. Under primary metabolic conditions, C. v...

journal_title：Applied microbiology and biotechnology

authors： Ichinose H,Nakamizo M,Wariishi H,Tanaka H

更新日期：2002-03-01 00:00:00

abstract：:Malaria is a widespread and infectious disease that is a leading cause of death in many parts of the world. Eradication of malaria has been a major world health goal for decades, but one that still remains elusive. Other diseases have been eradicated using vaccination, but traditional vaccination methods have thus far...

journal_title：Applied microbiology and biotechnology

authors： Jones CS,Luong T,Hannon M,Tran M,Gregory JA,Shen Z,Briggs SP,Mayfield SP

更新日期：2013-03-01 00:00:00

abstract：:Polyunsaturated fatty acids, docosahexaenoic acid (DHA, 22:6, n-3), eicosapentaenoic acid (EPA, 20:5, n-3), and arachidonic acid (ARA, 20:4 n-6), have multiple beneficial effects on human health and can be used as an important ingredient in dietary supplements, food, feed and pharmaceuticals. A variety of microorganis...

journal_title：Applied microbiology and biotechnology

authors： Wu L,Roe CL,Wen Z

更新日期：2013-09-01 00:00:00

abstract：:The utilization of monochlorobenzoate isomers (2-, 3- and 4-chlorobenzoate) by anaerobic microbial consortia in River Nile sediments was systematically evaluated under denitrifying, Fe-reducing, sulfidogenic and methanogenic conditions. Loss of all three chlorobenzoates was noted in denitrifying cultures; furthermore,...

journal_title：Applied microbiology and biotechnology

authors： Kazumi J,Häggblom MM,Young LY

更新日期：1995-10-01 00:00:00

abstract：:Tocopherols, collectively known as vitamin E, are lipophilic antioxidants, which are synthesized only by photosynthetic organisms. Due to their enormous potential to protect cells from oxidative damage, tocopherols are used, e.g., as nutraceuticals and additives in pharmaceuticals. The most biologically active form of...

journal_title：Applied microbiology and biotechnology

authors： Geipel K,Song X,Socher ML,Kümmritz S,Püschel J,Bley T,Ludwig-Müller J,Steingroewer J

更新日期：2014-03-01 00:00:00

abstract：:Aerobic granules are cultivated by a single bacterial strain, Acinetobacter calcoaceticus, in a sequencing batch reactor (SBR). This strain presents as a good phenol reducer and an efficient auto coagulator in the presence of phenol, mediated by heat-sensitive adhesins proteins. Stable 2.3-mm granules were formed in t...

journal_title：Applied microbiology and biotechnology

authors： Adav SS,Lee DJ

更新日期：2008-03-01 00:00:00

abstract：:Variable domains of heavy chains of camelid heavy-chain antibodies (VHHs) are known as nanobodies. Nanobodies are approximately 15 kDa in size with high affinity to their antigens. They can be easily manipulated and produced in microorganisms. In this study, an alpaca was immunized with purified green fluorescence pro...

journal_title：Applied microbiology and biotechnology

authors： Fang Z,Cao D,Qiu J

更新日期：2020-06-01 00:00:00

abstract：:Alkalophilic Bacillus sp. KSM-K16 produced three alkaline proteases, as detected by polyacrylamide gel electrophoresis (PAGE). The major protease, designated M protease, was recently purified to homogeneity and its properties were characterized. In the present study, two minor proteases, designated H protease and N pr...

journal_title：Applied microbiology and biotechnology

authors： Kobayashi T,Hakamada Y,Hitomi J,Koike K,Ito S

更新日期：1996-03-01 00:00:00

abstract：:Extracellular polymeric substance (EPS) is proposed to facilitate calcium ion supersaturation through its nucleation effect during the microbially induced calcium carbonate precipitation (MICP) process. However, the supersaturation effect of Ca2+ via EPS in MICP has not been clearly demonstrated. Enhanced exopolysacch...

journal_title：Applied microbiology and biotechnology

authors： Kim HJ,Shin B,Lee YS,Park W

更新日期：2017-08-01 00:00:00

abstract：:Despite airborne microorganisms representing a relevant fraction of atmospheric suspended particles, only a small amount of information is currently available on their abundance and diversity and very few studies have investigated the environmental factors influencing the structure of airborne bacterial communities. I...

journal_title：Applied microbiology and biotechnology

authors： Bertolini V,Gandolfi I,Ambrosini R,Bestetti G,Innocente E,Rampazzo G,Franzetti A

更新日期：2013-07-01 00:00:00

abstract：:Determining spatiotemporal gene expression and analyzing knockout mutant phenotypes have become powerful tools in elucidating the function of genes; however, genetic approaches for simultaneously inactivating a gene and monitoring its expression have not been reported in the literature. In this study, we designed a du...

journal_title：Applied microbiology and biotechnology

authors： Chen K,Xu X,Gu L,Hildreth M,Zhou R

更新日期：2015-02-01 00:00:00

abstract：:This study aimed to investigate the interaction between methane production performance and active microbial community dynamics at different loading rates by increasing influent substrate concentration. The model system was an upflow anaerobic sludge blanket (UASB) reactor using molasses wastewater. The active microbia...

journal_title：Applied microbiology and biotechnology

authors： Yun J,Lee SD,Cho KS

更新日期：2016-05-01 00:00:00

abstract：:The alpha-amylase gene (amy) from Streptomyces griseus IMRU 3570 and the beta-galactosidase gene (lac) from S. lividans were subcloned into Brevibacterium lactofermentum or B. lactofermentum/Escherichia coli shuttle vectors. The amy gene was not expressed in B. lactofermentum from its own promoter but was efficiently ...

journal_title：Applied microbiology and biotechnology

authors： Cadenas RF,Gil JA,Martín JF

更新日期：1992-12-01 00:00:00

abstract：:Two bioactive O-methylflavonoids, sakuranetin (7-O-methylnaringenin) and ponciretin (7-O-methylnaringenin), were synthesized in Escherichia coli. Sakuranetin inhibits germination of Magnaporthe grisea, and ponciretin is a potential inhibitor of Helicobacter pylori. To achieve this, we reconstructed the naringenin bios...

journal_title：Applied microbiology and biotechnology

authors： Kim MJ,Kim BG,Ahn JH

更新日期：2013-08-01 00:00:00

abstract：:Cytophaga hutchinsonii is a Gram-negative gliding bacterium, which can rapidly degrade crystalline cellulose via a novel strategy without any recognizable processive cellulases. Its mechanism of cellulose binding and degradation is still a mystery. In this study, the mutagenesis of C. hutchinsonii with the mariner-bas...

journal_title：Applied microbiology and biotechnology

authors： Ji X,Xu Y,Zhang C,Chen N,Lu X

更新日期：2012-10-01 00:00:00

abstract：:Three cytosolic NADPH-dependent flavin-associated proteins (Gox2107, Gox0502, and Gox2684) from Gluconobacter oxydans 621H were overproduced in Escherichia coli, and the recombinant enzymes were purified and characterized. Apparent native molecular masses of 65.2, 78.2, and 78.4 kDa were observed for Gox2107, Gox0502,...

journal_title：Applied microbiology and biotechnology

authors： Schweiger P,Gross H,Wesener S,Deppenmeier U

更新日期：2008-10-01 00:00:00

abstract：:Cell proliferation is achieved through numerous enzyme reactions. Temperature governs the activity of each enzyme, ultimately determining the optimal growth temperature. The synthesis of useful chemicals and fuels utilizes a fraction of available metabolic pathways, primarily central metabolic pathways including glyco...

journal_title：Applied microbiology and biotechnology

authors： Uchikura H,Toyoda K,Matsuzawa H,Mizuno H,Ninomiya K,Takahashi K,Inui M,Tsuge Y

更新日期：2020-08-01 00:00:00

abstract：:The recent, rapid increase in bacterial antimicrobial resistance has become a major public health concern. One approach to generate new classes of antibacterials is targeting virulence rather than the viability of bacteria. Proteins of the Dsb system, which play a key role in the virulence of many pathogenic microorga...

journal_title：Applied microbiology and biotechnology

authors： Bocian-Ostrzycka KM,Grzeszczuk MJ,Banaś AM,Jagusztyn-Krynicka EK

更新日期：2017-05-01 00:00:00

abstract：:Oil-producing fungus Mortierella alpina 1S-4 is an industrial strain. To determine its physiological properties and to clarify the biosynthetic pathways for polyunsaturated fatty acids, a transformation system for this fungus was established using a derivative of it, i.e., a ura5- mutant lacking orotate phosphoribosyl...

journal_title：Applied microbiology and biotechnology

authors： Takeno S,Sakuradani E,Murata S,Inohara-Ochiai M,Kawashima H,Ashikari T,Shimizu S

更新日期：2004-09-01 00:00:00

abstract：:The gene encoding NADP(+)-dependent L-1-amino-2-propanol dehydrogenase (AADH) of Rhodococcus erythropolis MAK154 was cloned and sequenced. A 780-bp nucleotide fragment was confirmed to be the gene encoding AADH by agreement of the N-terminal and internal amino acid sequences of the purified AADH. The gene (aadh) codes...

journal_title：Applied microbiology and biotechnology

authors： Kataoka M,Ishige T,Urano N,Nakamura Y,Sakuradani E,Fukui S,Kita S,Sakamoto K,Shimizu S

更新日期：2008-09-01 00:00:00

abstract：:Phosphate (P)-solubilizing microorganisms as a group form an important part of the microorganisms, which benefit plant growth and development. Growth promotion and increased uptake of phosphate are not the only mechanisms by which these microorganisms exert a positive effect on plants. Microbially mediated solubilizat...

journal_title：Applied microbiology and biotechnology

authors： Vassilev N,Vassileva M,Nikolaeva I

更新日期：2006-06-01 00:00:00

abstract：:This study was undertaken to assess the antifungal performance of three different Lactobacillus species.Experiments were conducted in vitro and in situ to extend the shelf life of wheat bread. Standard sourdough analyses were performed characterising acidity and carbohydrate levels. Overall, the strains showed good in...

journal_title：Applied microbiology and biotechnology

authors： Axel C,Brosnan B,Zannini E,Peyer LC,Furey A,Coffey A,Arendt EK

更新日期：2016-02-01 00:00:00

abstract：:Bacillus cereus 905, one of the plant growth-promoting rhizobacteria (PGPRs), is capable of colonizing wheat roots in a large population size. From previous studies, we learned that the sodA2-encoding manganese-containing superoxide dismutase (MnSOD2) is important for B. cereus 905 to survive in wheat rhizosphere. In ...

journal_title：Applied microbiology and biotechnology

authors： Gao T,Ding M,Wang Q

更新日期：2020-11-01 00:00:00