Vinyl ketone reduction by three distinct Gluconobacter oxydans 621H enzymes.

abstract：:In vertebrates and bacteria, heparosan the precursor of heparin is synthesized by glycosyltransferases via the stepwise addition of UDP-N-acetylglucosamine and UDP-glucuronic acid. As heparin-like molecules represent a great interest in the pharmaceutical area, the cryptic Pasteurella multocida heparosan synthase PmHS...

journal_title：Applied microbiology and biotechnology

authors： Chavaroche AA,Springer J,Kooy F,Boeriu C,Eggink G

更新日期：2010-02-01 00:00:00

abstract：:Eleutherosides, the phenylpropanoid and lignan glycosides, are the active ingredients accumulated in the roots and stems of Eleutherococcus species and in Eleutherococcus senticosus in particular. Syringin (=eleutheroside B) and (-) syringaresinol-di-O-β-D-glucoside (=eleutheroside E) appear as the most important bioa...

journal_title：Applied microbiology and biotechnology

authors： Murthy HN,Kim YS,Georgiev MI,Paek KY

更新日期：2014-09-01 00:00:00

abstract：:The behavior of Streptomyces peucetius var. caesius N47 was studied in a glucose limited chemostat with a complex cultivation medium. The steady-state study yielded the characteristic constants mu (max) over 0.10 h(-1), Y (XS) 0.536 g g(-1), and m(S) 0.54 mg g(-1) h(-1). The product of secondary metabolism, epsilon-rh...

journal_title：Applied microbiology and biotechnology

authors： Kiviharju K,Moilanen U,Leisola M,Eerikäinen T

更新日期：2007-01-01 00:00:00

abstract：:The medicinal mushroom Agaricus blazei produced high amounts of laccase (up to 5,000 units l(-1)) in a complex, agitated liquid medium based on tomato juice, while only traces of the enzyme (<100 units l(-1)) were detected in synthetic glucose-based medium. Purification of the enzyme required three chromatographic ste...

journal_title：Applied microbiology and biotechnology

authors： Ullrich R,Huong le M,Dung NL,Hofrichter M

更新日期：2005-05-01 00:00:00

abstract：:Economical production of medium-chain length polyhydroxyalkanoates (mcl-PHA) is dependent on efficient cultivation processes. This work describes growth and mcl-PHA synthesis characteristics of Pseudomonas putida LS46 when grown on medium-chain length fatty acids (octanoic acid) and lower-cost long-chain fatty acids (...

journal_title：Applied microbiology and biotechnology

authors： Blunt W,Dartiailh C,Sparling R,Gapes D,Levin DB,Cicek N

更新日期：2018-08-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae has been modified successfully for production of numerous metabolites and therapeutic proteins through metabolic engineering, but has not been utilized to date for the production of lipid-derived compounds. We developed a lipid metabolic engineering strategy in S. cerevisiae based up...

journal_title：Applied microbiology and biotechnology

authors： Dyer JM,Chapital DC,Kuan JW,Mullen RT,Pepperman AB

更新日期：2002-07-01 00:00:00

abstract：:Protein turnover through de novo synthesis is critical for sustainable cellular functions. We previously found that glucose consumption rate in Corynebacterium glutamicum under anaerobic conditions increased at temperature higher than the upper limit of growth temperature. Here, we showed that production of lactic and...

journal_title：Applied microbiology and biotechnology

authors： Uchikura H,Ninomiya K,Takahashi K,Tsuge Y

更新日期：2020-05-01 00:00:00

abstract：:Rhodovulum sulfidophilum DSM-1374 is a potential producer of polyester when growing in phototrophic conditions. The present study investigated on a polyester product (P3HB) by culturing Rhodovulum sulfidophilum DSM-1374 in two different photobioreactors (PBR-1 and PBR-2) both with 4-L working volumes. PBR-1 is equippe...

journal_title：Applied microbiology and biotechnology

authors： Carlozzi P,Di Lorenzo T,Ghanotakis DF,Touloupakis E

更新日期：2020-03-01 00:00:00

abstract：:The upstream region of the cellobiohydrolase gene cbhA of Clostridium thermocellum F7 was sequenced. It was found that this region contains the previously sequenced gene celK encoding an enzyme closely related to CbhA (cellulosomal subunit S3). The presence of a putative transcription terminator in the 524-bp intergen...

journal_title：Applied microbiology and biotechnology

authors： Zverlov VV,Velikodvorskaya GA,Schwarz WH,Kellermann J,Staudenbauer WL

更新日期：1999-06-01 00:00:00

abstract：:A transcriptional activator for actinorhodin biosynthesis, AtrA, was previously characterized in Streptomyces coelicolor A3(2), and an orthologue of atrA, named aveI, is identified in the Streptomyces avermitilis NRRL8165 genome (Uguru et al., Mol Microbiol, 58:131-150, 2005). In this study, genetic and functional cha...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Lu Y,Chen J,Zhang W,Shu D,Qin Z,Yang S,Jiang W

更新日期：2008-08-01 00:00:00

abstract：:Glucose 6-phosphate dehydrogenase (G6PDH) is one of the most important dehydrogenases responsible for generating reduced NADPH for anabolism and is also the rate-limiting enzyme in the Entner-Doudoroff pathway. For in vitro biocatalysis, G6PDH must possess both high activity and good thermostability due to requirement...

journal_title：Applied microbiology and biotechnology

authors： Huang R,Chen H,Zhou W,Ma C,Zhang YP

更新日期：2018-04-01 00:00:00

abstract：:Batch and continuous cultivation of Anaerobiospirillum succiniciproducens were systematically studied for the production of succinic acid from whey. Addition of 2.5 g l(-1) yeast extract and 2.5 g l(-1) polypeptone per 10 g l(-1) whey was most effective for succinic acid production from both treated and nontreated whe...

journal_title：Applied microbiology and biotechnology

authors： Lee PC,Lee WG,Kwon S,Lee SY,Chang HN

更新日期：2000-07-01 00:00:00

abstract：:Production of (R)-3-hydroxybutyric acid [(R)-3-HB] by strain Halomonas sp. KM-1 under successive aeration conditions was investigated. The first aerobic condition allowed both cell growth and intracellular storage of poly-(R)-3-hydroxybutyric acid (PHB). The second microaerobic condition, achieved by reducing the cult...

journal_title：Applied microbiology and biotechnology

authors： Kawata Y,Kawasaki K,Shigeri Y

更新日期：2012-11-01 00:00:00

abstract：:Cryparin is an abundant cell-wall-associated hydrophobin of Cryphonectria parasitica. Although cryparin is encoded as a single copy gene, it is the most abundant protein produced by this fungus when grown in liquid culture. Studies to characterize the transcriptional regulatory element(s) found that the fragment betwe...

journal_title：Applied microbiology and biotechnology

authors： Kwon BR,Kim MJ,Park JA,Chung HJ,Kim JM,Park SM,Yun SH,Yang MS,Kim DH

更新日期：2009-05-01 00:00:00

abstract：:Oleaginous yeasts (18 strains) were grown in ethanol media under various cultivation conditions (33 biomass samples). It was found that lipid and lipid-free fractions of dry biomass have elemental composition and biomass reductivity very close to values which can be considered as biological constants. The energy conte...

journal_title：Applied microbiology and biotechnology

authors： Minkevich IG,Dedyukhina EG,Chistyakova TI

更新日期：2010-10-01 00:00:00

abstract：:Tocopherols, collectively known as vitamin E, are lipophilic antioxidants, which are synthesized only by photosynthetic organisms. Due to their enormous potential to protect cells from oxidative damage, tocopherols are used, e.g., as nutraceuticals and additives in pharmaceuticals. The most biologically active form of...

journal_title：Applied microbiology and biotechnology

authors： Geipel K,Song X,Socher ML,Kümmritz S,Püschel J,Bley T,Ludwig-Müller J,Steingroewer J

更新日期：2014-03-01 00:00:00

abstract：:Bacillus cereus 905, one of the plant growth-promoting rhizobacteria (PGPRs), is capable of colonizing wheat roots in a large population size. From previous studies, we learned that the sodA2-encoding manganese-containing superoxide dismutase (MnSOD2) is important for B. cereus 905 to survive in wheat rhizosphere. In ...

journal_title：Applied microbiology and biotechnology

authors： Gao T,Ding M,Wang Q

更新日期：2020-11-01 00:00:00

abstract：:A bacterial strain C21 isolated from constructed wetland soil was identified as Arthrobacter sp. based on 16S rRNA gene sequence analysis and physio-biochemical characteristics and was capable of utilizing di-n-butyl phthalate (DBP) as a carbon and energy source for growth. Strain C21 can also utilize other phthalates...

journal_title：Applied microbiology and biotechnology

authors： Wen ZD,Gao DW,Wu WM

更新日期：2014-05-01 00:00:00

abstract：:Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compou...

journal_title：Applied microbiology and biotechnology

authors： Ravi K,García-Hidalgo J,Gorwa-Grauslund MF,Lidén G

更新日期：2017-06-01 00:00:00

abstract：:We have fused the epidermal growth factor (EGF) to the amino terminus of Pseudomonas exotoxin A (PE) to create a cytotoxic agent, designated EGF-PE, which preferentially kills EGF-receptor-bearing cells. In this study, we analyzed the effect of the Ia domain, the binding domain of PE on the cytotoxicity of EGF-PE towa...

journal_title：Applied microbiology and biotechnology

authors： Liao CW,Hseu TH,Hwang J

更新日期：1995-07-01 00:00:00

abstract：:The Ser-170 residue of a trypsin-like enzyme from Streptomyces fradiae (SFT), which is considered to be the active-site serine, was replaced with alanine by site-directed mutagenesis to improve the affinity chromatography step for a Kazal-type trypsin inhibitor pancreatic secretory trypsin inhibitor (PSTI). The result...

journal_title：Applied microbiology and biotechnology

authors： Katoh T,Kikuchi N,Nagata K,Yoshida N

更新日期：1996-08-01 00:00:00

abstract：:Microbial strains of the genera Dietzia, Micrococcus, Pseudomonas, Rhodococcus, Gordonia, Streptomyces, Pseudomonas, Bacillus, Penicillium, Rhodotorula and Lactobacillus were screened for the ability to convert chalcones. Synthesis of chalcones was performed by the Claisen-Schmidt reaction. There were three groups of ...

journal_title：Applied microbiology and biotechnology

authors： Stompor M,Kałużny M,Żarowska B

更新日期：2016-10-01 00:00:00

abstract：:The dynamics of bacterial communities play an important role in solid-state fermentation (SSF). Poly-γ-glutamic acid (γ-PGA) was produced by Bacillus amyloliquefaciens C1 in SSF using dairy manure compost and monosodium glutamate production residuals as basic substrates. The production of γ-PGA reached a maximum of 0....

journal_title：Applied microbiology and biotechnology

authors： Yong X,Cui Y,Chen L,Ran W,Shen Q,Yang X

更新日期：2011-11-01 00:00:00

abstract：:Agar is a major polysaccharide of red algal cells and is mainly decomposed into neoagarobiose by the co-operative effort of β-agarases. Neoagarobiose is hydrolyzed into monomers, D-galactose and 3,6-anhydro-L-galactose, via a microbial oxidative process. Therefore, the enzyme, 1,3-α-3,6-anhydro-L-galactosidase (α-neoa...

journal_title：Applied microbiology and biotechnology

authors： Asghar S,Lee CR,Park JS,Chi WJ,Kang DK,Hong SK

更新日期：2018-10-01 00:00:00

abstract：:Despite being a yellow pigment visible to the human eye, coelimycin (CPK) remained to be an undiscovered secondary metabolite for over 50 years of Streptomyces research. Although the function of this polyketide is still unclear, we now know that its "cryptic" nature is attributed to a very complex and precise mechanis...

journal_title：Applied microbiology and biotechnology

authors： Bednarz B,Kotowska M,Pawlik KJ

更新日期：2019-08-01 00:00:00

abstract：:Alcaligenesfaecalis (CCT 7145) was isolated from an Amazonian soil sample after an enrichment process to select for phenol-degrading microorganisms. The isolate was labeled with the green fluorescent protein (gfp) gene. The gfp-transformed cells were easily detected using a hand-held UV transilluminator and their taxo...

journal_title：Applied microbiology and biotechnology

authors： Bastos AE,Cassidy MB,Trevors JT,Lee H,Rossi A

更新日期：2001-07-01 00:00:00

abstract：:Monascus pigments (Mps) are a group of azaphilonic secondary metabolites produced by Monascus spp. via a polyketide pathway. A mutant deleted an about 30 kb region of Mps gene cluster from Monascus ruber M7 was isolated previously, which produces a high amount of a light yellow pigment. The current study revealed that...

journal_title：Applied microbiology and biotechnology

authors： Liu J,Zhou Y,Yi T,Zhao M,Xie N,Lei M,Liu Q,Shao Y,Chen F

更新日期：2016-08-01 00:00:00

abstract：:Brazzein is an intensely sweet-tasting plant protein with good stability, which makes it an attractive alternative to sucrose. A brazzein gene has been designed, synthesized, and expressed in Escherichia coli at 30 degrees C to yield brazzein in a soluble form and in considerable quantity. Antibodies have been produce...

journal_title：Applied microbiology and biotechnology

authors： Berlec A,Jevnikar Z,Majhenic AC,Rogelj I,Strukelj B

更新日期：2006-11-01 00:00:00

abstract：:In the Funding section, the following statement is missing: The MACS cohort study was supported by the NIH, National Institute of Allergy and Infectious Diseases grant U01-AI35041. ...

journal_title：Applied microbiology and biotechnology

authors： Palma ML,Garcia-Bates TM,Martins FS,Douradinha B

更新日期：2019-07-01 00:00:00

abstract：:Using a randomly-cloned, HindIII-digested, chromosomal fragment from Lactococcus lactis subsp. lactis LM0230, first-generation lactococcal integrative cloning vectors were developed. Through dideoxy DNA sequence analysis, the cloned chromosomal DNA fragment was determined to be 1026 base pairs. Southern hybridization ...

journal_title：Applied microbiology and biotechnology

authors： McIntyre DA,Harlander SK

更新日期：1993-11-01 00:00:00