Effects of pH, temperature and salinity on P3HB synthesis culturing the marine Rhodovulum sulfidophilum DSM-1374.

abstract：:Production of a novel antimicrobial tropolone, beta-thujaplicin, in Cupressus lusitanica suspension cultures was studied by using a variety of chemicals and fungal elicitors. Sodium alginate, chitin, and methyl jasmonate resulted in 2-, 2.5-, and 3-fold higher beta-thujaplicin production, respectively, than in the con...

journal_title：Applied microbiology and biotechnology

authors： Zhao J,Fujita K,Yamada J,Sakai K

更新日期：2001-04-01 00:00:00

abstract：:A characteristic mucosal immune response involves the production of antigen-specific secretory immunoglobulin A (SIgA) antibodies. In order to study transcytosis by mimicking the SIgA secretion and to screen for SIgA secretion-promoting substances, we developed a model system of a transfected Madin-Darby canine kidney...

journal_title：Applied microbiology and biotechnology

authors： Zuo T,Feng X,Zhang N,Xue C,Tang QJ

更新日期：2015-07-01 00:00:00

abstract：:The activities of seven hydrolytic enzymes (L-alanine aminopeptidase, esterase, α-and β-glucosidase, phosphomonoesterase, phosphodiesterase, sulfatase) were monitored during 1 year in parallel and serial treatment units of the biological stage of a communal wastewater treatment plant. The spatial homogeneity of enzyme...

journal_title：Applied microbiology and biotechnology

authors： Kreutz JA,Böckenhüser I,Wacht M,Fischer K

更新日期：2016-08-01 00:00:00

abstract：:Translational lacZ fusions to the promoters of the parasporal, crystal protein (binary toxin) and 100-kDa ADP-ribosylating mosquitocidal toxin genes of Bacillus sphaericus were prepared and expression of the toxin genes monitored as beta-galactosidase activity. Transcription of the crystal protein gene fusion began im...

journal_title：Applied microbiology and biotechnology

authors： Ahmed HK,Mitchell WJ,Priest FG

更新日期：1995-05-01 00:00:00

abstract：:Phytoplankton blooms are predictable features of marine and freshwater habitats. Despite a good knowledge base of the environmental factors controlling blooms, complex interactions between the bacterial and archaeal communities and phytoplankton bloom taxa are only now emerging. Here, the current research on bacterial...

journal_title：Applied microbiology and biotechnology

authors： Song L

更新日期：2017-06-01 00:00:00

abstract：:A beta-lactamase-free penicillin amidase from Alcaligenes sp. active against various beta-lactams was purified to homogeneity. The enzyme can hydrolyze penicillin G to 6-amino penicillanic acid (6-APA) and furnish penicillin G from 6-APA and phenyl acetic acid by condensation. The penicillin amidase is a heterodimer o...

journal_title：Applied microbiology and biotechnology

authors： Das S,Gayen JR,Pal A,Ghosh K,Rosazza JP,Samanta TB

更新日期：2004-08-01 00:00:00

abstract：:Bacterial polyhedral organelles are extremely large macromolecular complexes consisting of metabolic enzymes encased within a multiprotein shell that is somewhat reminiscent of a viral capsid. Recent investigations suggest that polyhedral organelles are widely used by bacteria for optimizing metabolic processes. The d...

journal_title：Applied microbiology and biotechnology

authors： Bobik TA

更新日期：2006-05-01 00:00:00

abstract：:Pyranose 2-oxidase (P2O) was purified 43-fold to apparent homogeneity from the basidiomycete Phanerochaete chrysosporium using liquid chromatography on phenyl Sepharose, Mono Q (twice) and phenyl Superose. The native enzyme has a molecular mass of about 250 kDa (based on native PAGE) and is composed of four identical ...

journal_title：Applied microbiology and biotechnology

authors： Artolozaga MJ,Kubátová E,Volc J,Kalisz HM

更新日期：1997-05-01 00:00:00

abstract：:Infectious bursal disease is a widely spread threatening contagious viral infection of chickens that induces major damages to the Bursa of Fabricius and leads to severe immunosuppression in young birds causing significant economic losses for poultry farming. The etiological agent is the infectious bursal disease virus...

journal_title：Applied microbiology and biotechnology

authors： Rage E,Marusic C,Lico C,Baschieri S,Donini M

更新日期：2020-03-01 00:00:00

abstract：:Three cytosolic NADPH-dependent flavin-associated proteins (Gox2107, Gox0502, and Gox2684) from Gluconobacter oxydans 621H were overproduced in Escherichia coli, and the recombinant enzymes were purified and characterized. Apparent native molecular masses of 65.2, 78.2, and 78.4 kDa were observed for Gox2107, Gox0502,...

journal_title：Applied microbiology and biotechnology

authors： Schweiger P,Gross H,Wesener S,Deppenmeier U

更新日期：2008-10-01 00:00:00

abstract：:κ-Carrageenases exhibit apparent distinctions in gene sequence, molecular weight, enzyme properties, and posttranslational processes. In this study, a new κ-carrageenase gene named cgkZ was cloned from the marine bacterium Zobellia sp. ZM-2. The gene comprised an open reading frame of 1,638 bp and encoded 545 amino ac...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Li G,Mo Z,Mou H

更新日期：2013-12-01 00:00:00

abstract：:A customer- and environment-friendly method for the decolorization azo dyes was developed. Azoreductases could be used both to bleach hair dyed with azo dyes and to reduce dyes in vat dyeing of textiles. A new reduced nicotinamide adenine dinucleotide-dependent azoreductase of Bacillus cereus, which showed high potent...

journal_title：Applied microbiology and biotechnology

authors： Pricelius S,Held C,Murkovic M,Bozic M,Kokol V,Cavaco-Paulo A,Guebitz GM

更新日期：2007-11-01 00:00:00

abstract：:Extracellular manganese peroxidase (MnP) was purified from the compost extract of Agaricus bisporus using anion exchange chromatography, gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two forms (MnP1 and MnP2) were separated by isoelectric focusing and their isoelectric points...

journal_title：Applied microbiology and biotechnology

authors： Lankinen VP,Bonnen AM,Anton LH,Wood DA,Kalkkinen N,Hatakka A,Thurston CF

更新日期：2001-03-01 00:00:00

abstract：:Fatty acid desaturases play vital roles in the synthesis of unsaturated fatty acids. In this study, Δ12 and Δ12/Δ15 fatty acid desaturases of the oleaginous yeast Lipomyces starkeyi, termed LsFad2 and LsFad3, respectively, were identified and characterized. Saccharomyces cerevisiae expressing LsFAD2 converted oleic ac...

journal_title：Applied microbiology and biotechnology

authors： Matsuzawa T,Maehara T,Kamisaka Y,Ara S,Takaku H,Yaoi K

更新日期：2018-10-01 00:00:00

abstract：:Developing the production of ectomycorrhizal (ECM) mushrooms in forest has become a challenge. Only a few ECM species are currently cultivable. Controlled mycorrhization practices offer promising advance to produce currently uncultivable ECM mushrooms. The persistence of the production of edible species, either cultiv...

journal_title：Applied microbiology and biotechnology

authors： Savoie JM,Largeteau ML

更新日期：2011-02-01 00:00:00

abstract：:Bacterial cells are protected by an exoskeleton, the stabilizing and shape-maintaining cell wall, consisting of the complex macromolecule peptidoglycan. In view of its function, it could be assumed that the cell wall is a static structure. In truth, however, it is steadily broken down by peptidoglycan-cleaving enzymes...

journal_title：Applied microbiology and biotechnology

authors： Reith J,Mayer C

更新日期：2011-10-01 00:00:00

abstract：:To develop microbial production method for prenyl alcohols (e.g., (E,E)-farnesol (FOH), (E)-nerolidol (NOH), and (E,E,E)-geranylgeraniol (GGOH)), the genes encoding enzymes in the mevalonate and prenyl diphosphate pathways were overexpressed in Saccharomyces cerevisiae, and the resultant transformants were evaluated a...

journal_title：Applied microbiology and biotechnology

authors： Ohto C,Muramatsu M,Obata S,Sakuradani E,Shimizu S

更新日期：2009-04-01 00:00:00

abstract：:Medicinal plants of the Dendrobium genus are highly prized, and hence, methodologies have been developed to authenticate Dendrobium drugs from its adulterants. Many bioactive constituents of Dendrobium species have been identified. The macromolecules included lectins; the enzymes chalcone synthase, sucrose synthase, a...

journal_title：Applied microbiology and biotechnology

authors： Ng TB,Liu J,Wong JH,Ye X,Wing Sze SC,Tong Y,Zhang KY

更新日期：2012-03-01 00:00:00

abstract：:Myogenesis involves the determination of progenitor cells to myoblasts, their fusion to yield multinuclear myotubes, and the maturation of myotubes to muscle fibres. This development is reflected in a time pattern of gene expression, e.g. of genes coding for desmin, the myogenic factors myogenin and myoD, the acetylch...

journal_title：Applied microbiology and biotechnology

authors： Bardouille C,Lehmann J,Heimann P,Jockusch H

更新日期：2001-05-01 00:00:00

abstract：:In the recent years, anaerobic membrane bioreactor (AnMBR) technology is being considered as a very attractive alternative for wastewater treatment due to the striking advantages such as upgraded effluent quality. However, fouling control is still a problem for the application of AnMBR. This study investigated the per...

journal_title：Applied microbiology and biotechnology

authors： Li N,Hu Y,Lu YZ,Zeng RJ,Sheng GP

更新日期：2016-07-01 00:00:00

abstract：:Microalgal starch is a potential feedstock for biofuel production. Nutrient stress is widely used to stimulate starch accumulation in microalgae. Cell growth and starch accumulation in the marine green microalga Tetraselmis subcordiformis were evaluated under extracellular phosphorus deprivation with initial cell dens...

journal_title：Applied microbiology and biotechnology

authors： Yao CH,Ai JN,Cao XP,Xue S

更新日期：2013-07-01 00:00:00

abstract：:This review presents developments and applications in bioleaching and mineral biooxidation since publication of a previous mini review in 2003 (Olson et al. Appl Microbiol Biotechnol 63:249-257, 2003). There have been discoveries of newly identified acidophilic microorganisms that have unique characteristics for effec...

journal_title：Applied microbiology and biotechnology

authors： Brierley CL,Brierley JA

更新日期：2013-09-01 00:00:00

abstract：:Staphylococcus aureus causes severe infections and among all methicillin-resistant S. aureus (MRSA) remains a great challenge in spite of decade research of antibacterial compounds. Even though some synthetic antibiotics have been developed, they are not effective against MRSA, and hence, there is a search for natural...

journal_title：Applied microbiology and biotechnology

authors： Sinsinwar S,Vadivel V

更新日期：2020-10-01 00:00:00

abstract：:Alcaligenesfaecalis (CCT 7145) was isolated from an Amazonian soil sample after an enrichment process to select for phenol-degrading microorganisms. The isolate was labeled with the green fluorescent protein (gfp) gene. The gfp-transformed cells were easily detected using a hand-held UV transilluminator and their taxo...

journal_title：Applied microbiology and biotechnology

authors： Bastos AE,Cassidy MB,Trevors JT,Lee H,Rossi A

更新日期：2001-07-01 00:00:00

abstract：:Sophorolipids are surface-active compounds synthesized by a selected number of yeast species. They have been known for over 40 years, but because of growing environmental awareness, they recently regained attention as biosurfactants due to their biodegradability, low ecotoxicity, and production based on renewable reso...

journal_title：Applied microbiology and biotechnology

authors： Van Bogaert IN,Saerens K,De Muynck C,Develter D,Soetaert W,Vandamme EJ

更新日期：2007-08-01 00:00:00

abstract：:Cloning and biochemical characterization of a novel extracellular medium-chain-length polyhydroxyalkanoate (mcl-PHA) depolymerase from Streptomyces exfoliatus K10 DSMZ 41693 are described. The primary structure of the depolymerase (PhaZSex2) includes the lipase consensus sequence (serine-histidine-aspartic acid) which...

journal_title：Applied microbiology and biotechnology

authors： Martínez V,de Santos PG,García-Hidalgo J,Hormigo D,Prieto MA,Arroyo M,de la Mata I

更新日期：2015-11-01 00:00:00

abstract：:Plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target of a range of synthetic β-triketone herbicides that are currently used commercially. Their mode of action is based on an irreversible inhibition of HPPD. Therefore, this inhibitory capacity was used to develop a whole-cell colorimetric bioassay w...

journal_title：Applied microbiology and biotechnology

authors： Rocaboy-Faquet E,Noguer T,Romdhane S,Bertrand C,Dayan FE,Barthelmebs L

更新日期：2014-08-01 00:00:00

abstract：:Natural attenuation maybe a cost-efficient option for bioremediation of contaminated sites but requires knowledge about the activity of degrading microbes under in situ conditions. In order to link microbial activity to the spatial distribution of contaminant degraders, we combined the recently improved in situ microc...

journal_title：Applied microbiology and biotechnology

authors： Schurig C,Mueller CW,Höschen C,Prager A,Kothe E,Beck H,Miltner A,Kästner M

更新日期：2015-01-01 00:00:00

abstract：:His-Asn-His (HNH) proteins are a very common family of small nucleic acid-binding proteins that are generally associated with endonuclease activity and are found in all kingdoms of life. Although HNH endonucleases from mesophiles have been widely investigated, the biochemical functions of HNH endonucleases from thermo...

journal_title：Applied microbiology and biotechnology

authors： Zhang L,Huang Y,Xu D,Yang L,Qian K,Chang G,Gong Y,Zhou X,Ma K

更新日期：2016-09-01 00:00:00

abstract：:L-Ribose is a rare and expensive sugar that can be used as a precursor for the production of L-nucleoside analogues, which are used as antiviral drugs. In this work, we describe a novel way of producing L-ribose from the readily available raw material L-arabinose. This was achieved by introducing L-ribose isomerase ac...

journal_title：Applied microbiology and biotechnology

authors： Helanto M,Kiviharju K,Granström T,Leisola M,Nyyssölä A

更新日期：2009-05-01 00:00:00