Identification and characterization of Δ12 and Δ12/Δ15 bifunctional fatty acid desaturases in the oleaginous yeast Lipomyces starkeyi.

Abstract:

:Fatty acid desaturases play vital roles in the synthesis of unsaturated fatty acids. In this study, Δ12 and Δ12/Δ15 fatty acid desaturases of the oleaginous yeast Lipomyces starkeyi, termed LsFad2 and LsFad3, respectively, were identified and characterized. Saccharomyces cerevisiae expressing LsFAD2 converted oleic acid (C18:1) to linoleic acid (C18:2), while a strain of LsFAD3-expressing S. cerevisiae converted oleic acid to linoleic acid, and linoleic acid to α-linolenic acid (C18:3), indicating that LsFad2 and LsFad3 were Δ12 and bifunctional Δ12/Δ15 fatty acid desaturases, respectively. The overexpression of LsFAD2 in L. starkeyi caused an accumulation of linoleic acid and a reduction in oleic acid levels. In contrast, overexpression of LsFAD3 induced the production of α-linolenic acid. Deletion of LsFAD2 and LsFAD3 induced the accumulation of oleic acid and linoleic acid, respectively. Our findings are significant for the commercial production of polyunsaturated fatty acids, such as ω-3 polyunsaturated fatty acids, in L. starkeyi.

authors

Matsuzawa T,Maehara T,Kamisaka Y,Ara S,Takaku H,Yaoi K

doi

10.1007/s00253-018-9345-2

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

8817-8826

issue

20

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-018-9345-2

journal_volume

102

pub_type

杂志文章
  • Increased antifungal and chitinase specific activities of Trichoderma harzianum CECT 2413 by addition of a cellulose binding domain.

    abstract::Trichoderma harzianum is a widely distributed soil fungus that antagonizes numerous fungal phytopathogens. The antagonism of T. harzianum usually correlates with the production of antifungal activities including the secretion of fungal cell walls that degrade enzymes such as chitinases. Chitinases Chit42 and Chit33 fr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-003-1538-6

    authors: Limón MC,Chacón MR,Mejías R,Delgado-Jarana J,Rincón AM,Codón AC,Benítez T

    更新日期:2004-06-01 00:00:00

  • Acid phosphatase production by recombinant Arxula adeninivorans.

    abstract::Acid phosphatase production by recombinant Arxula adeninivorans was carried out in submerged fermentation. Using the Plackett-Burman design, three fermentation variables (pH, sucrose concentration, and peptone concentration) were identified to significantly affect acid phosphatase and biomass production, and these wer...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-1021-x

    authors: Minocha N,Kaur P,Satyanarayana T,Kunze G

    更新日期:2007-08-01 00:00:00

  • Immunogenic characterization and protective efficacy of recombinant CsgA, major subunit of curli fibers, against Vibrio parahaemolyticus.

    abstract::Vibrio parahaemolyticus is one of the major pathogens responsible for vibriosis and zoonotic infections in teleosts, marine invertebrates, and also humans through consumption of contaminated or unprocessed seafood. Emergence of resistance against current accessible antibiotics and spread to the food chain and environm...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-11038-4

    authors: Karan S,Choudhury D,Dixit A

    更新日期:2021-01-01 00:00:00

  • Engineered phytases for emerging biotechnological applications beyond animal feeding.

    abstract::Phytases are important industrial enzymes able to catalyze the release of up to six phosphates from phytate in a stepwise hydrolysis reaction. Phytases are almost exclusively used as a feed supplement. However, phytases are also used in human nutrition, food processing, non-food industrial products, and emerging appli...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-019-09962-1

    authors: Herrmann KR,Ruff AJ,Infanzón B,Schwaneberg U

    更新日期:2019-08-01 00:00:00

  • Investigation of antimicrobial physiology of orthorhombic and monoclinic nanoallotropes of sulfur at the interface of transcriptome and metabolome.

    abstract::Nanosized elemental sulfur (ES) is already reported to exert superior antimicrobial efficacy than micron-sized ES, which encourages their use in drugs and therapeutics. The aim of the present study is to explore the possible route and mode of antimicrobial action of orthorhombic (α-SNPs) and monoclinic (β-SNPs) allotr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-4789-x

    authors: Roy Choudhury S,Mandal A,Ghosh M,Basu S,Chakravorty D,Goswami A

    更新日期:2013-07-01 00:00:00

  • Effect of anaerobic promoters on the microaerobic production of polyhydroxybutyrate (PHB) in recombinant Escherichia coli.

    abstract::Nine anaerobic promoters were cloned and constructed upstream of PHB synthesis genes phbCAB from Ralstonia eutropha for the micro- or anaerobic PHB production in recombinant Escherichia coli. Among the promoters, the one for alcohol dehydrogenase (PadhE) was found most effective. Recombinant E. coli JM 109 (pWCY09) ha...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-008-1816-4

    authors: Wei XX,Shi ZY,Yuan MQ,Chen GQ

    更新日期:2009-03-01 00:00:00

  • Biosynthesis of pyruvic acid from glucose by Blastobotrys adeninivorans.

    abstract::The ability of taxonomically different yeasts to synthesize pyruvic acid (PA) from glucose was studied. The study showed that many yeasts are able to produce PA from glucose under the condition of growth limitation by thiamine. This ability was found in the yeast Blastobotrys adeninivorans for the first time. The prod...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7618-1

    authors: Kamzolova SV,Morgunov IG

    更新日期:2016-09-01 00:00:00

  • Directed evolution of an acid Yersinia mollaretii phytase for broadened activity at neutral pH.

    abstract::Phytases are phosphohydrolases that initiate the sequential hydrolysis of phosphate from phytate, which is the main storage form of phosphorous in numerous plant seeds, especially in cereals and grains. Phytate is indigestible for most monogastric animals, such as poultry, swine, fish, and humans; therefore, microbial...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9308-7

    authors: Körfer G,Novoa C,Kern J,Balla E,Grütering C,Davari MD,Martinez R,Vojcic L,Schwaneberg U

    更新日期:2018-11-01 00:00:00

  • The antiviral protein cyanovirin-N: the current state of its production and applications.

    abstract::Human immunodeficiency virus (HIV)/AIDS continues to spread worldwide, and most of the HIV-infected people living in developing countries have little or no access to highly active antiretroviral therapy. The development of efficient and low-cost microbicides to prevent sexual transmission of HIV should be given high p...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-010-2470-1

    authors: Xiong S,Fan J,Kitazato K

    更新日期:2010-04-01 00:00:00

  • Improvement of sludge digestate biodegradability by thermophilic bioaugmentation.

    abstract::The sludge digestate stabilized by mesophilic anaerobic digestion was further degraded through thermophilic anaerobic digestion using 0-10 % (v/v) of thermophilic, proteolytic Coprothermobacter proteolyticus, and/or methanogenic granular sludge. The results demonstrated that the temperature shift to thermophilic condi...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-4977-8

    authors: Lü F,Li T,Wang T,Shao L,He P

    更新日期:2014-01-01 00:00:00

  • Development of an improved phenylacetaldehyde reductase mutant by an efficient selection procedure.

    abstract::Chiral alcohols are valuable as diverse chemicals and synthetic intermediate materials. Phenylacetaldehyde reductase (PAR) is an enzyme that converts a wide variety of ketones into chiral alcohols with high optical purity. When an alcohol such as 2-propanol is used as a hydrogen donor, PAR itself will also mediate the...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5406-8

    authors: Makino Y,Itoh N

    更新日期:2014-05-01 00:00:00

  • Introduction of green fluorescent protein gene into phenol-degrading Alcaligenes faecalis cells and their monitoring in phenol-contaminated soil.

    abstract::Alcaligenesfaecalis (CCT 7145) was isolated from an Amazonian soil sample after an enrichment process to select for phenol-degrading microorganisms. The isolate was labeled with the green fluorescent protein (gfp) gene. The gfp-transformed cells were easily detected using a hand-held UV transilluminator and their taxo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530100614

    authors: Bastos AE,Cassidy MB,Trevors JT,Lee H,Rossi A

    更新日期:2001-07-01 00:00:00

  • Growth and biomass productivity of Scenedesmus vacuolatus on a twin layer system and a comparison with other types of cultivations.

    abstract::Scenedesmus is a genus of microalgae employed for several industrial uses. Industrial cultivations are performed in open ponds or in closed photobioreactors (PBRs). In the last years, a novel type of PBR based on immobilized microalgae has been developed termed porous substrate photobioreactors (PSBR) to achieve signi...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8515-y

    authors: Carbone DA,Olivieri G,Pollio A,Gabriele,Melkonian M

    更新日期:2017-12-01 00:00:00

  • Biosynthesis of nervonic acid and perspectives for its production by microalgae and other microorganisms.

    abstract::Nervonic acid (NA) is a major very long-chain monounsaturated fatty acid found in the white matter of mammalian brains, which plays a critical role in the treatment of psychotic disorders and neurological development. In the nature, NA has been synthesized by a handful plants, fungi, and microalgae. Although the metab...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-018-8859-y

    authors: Fan Y,Meng HM,Hu GR,Li FL

    更新日期:2018-04-01 00:00:00

  • Engineering of the cytochrome P450 monooxygenase system for benzyl maltol hydroxylation.

    abstract::Maltol derivatives are utilized in a variety of fields due to their metal-chelating abilities, and modification of the 2-methyl side chain is known to effectively expand their functional diversity. In the present study, microbial enzymes were screened for hydroxylating activity towards the 2-methyl group in a maltol d...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8414-2

    authors: Kozono I,Mihara K,Minagawa K,Hibi M,Ogawa J

    更新日期:2017-09-01 00:00:00

  • Substrate specificity of Stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid.

    abstract::An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regi...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-1280-6

    authors: Yu IS,Yeom SJ,Kim HJ,Lee JK,Kim YH,Oh DK

    更新日期:2008-02-01 00:00:00

  • Functions and impact of tal-like genes in animals with regard to applied aspects.

    abstract::A large number of DNAs in eukaryote genomes can code for atypical transcripts, and their functions are controversial. It has been reported that the transcripts contain many small open reading frames (sORFs), which were originally considered as non-translatable RNAs. However, increasing evidence has suggested that some...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-018-9159-2

    authors: Zhu M,Hu X,Cao G,Xue R,Gong C

    更新日期:2018-08-01 00:00:00

  • Geobacillus strains that have potential value in microbial enhanced oil recovery.

    abstract::Bacteria from the genus Geobacillus are generally obligately thermophilic, with a unique bioenergy production capacity and unique enzymes. Geobacillus species were isolated primarily from hot springs, oilfields, and associated soils. They often exhibit unique survival patterns in these extreme oligotrophic environment...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-019-10115-7

    authors: Lin JH,Zhang KC,Tao WY,Wang D,Li S

    更新日期:2019-10-01 00:00:00

  • Enrichment of anaerobic nitrate-dependent methanotrophic 'Candidatus Methanoperedens nitroreducens' archaea from an Italian paddy field soil.

    abstract::Paddy fields are a significant source of methane and contribute up to 20% of total methane emissions from wetland ecosystems. These inundated, anoxic soils featuring abundant nitrogen compounds and methane are an ideal niche for nitrate-dependent anaerobic methanotrophs. After 2 years of enrichment with a continuous s...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8416-0

    authors: Vaksmaa A,Guerrero-Cruz S,van Alen TA,Cremers G,Ettwig KF,Lüke C,Jetten MSM

    更新日期:2017-09-01 00:00:00

  • Enzymatic (R)-phenylacetylcarbinol production in a benzaldehyde emulsion system with Candida utilis cells.

    abstract::Recent progress in enzymatic (R)-phenylacetylcarbinol (PAC) production has established the need for low cost and efficient biocatalyst preparation. Pyruvate decarboxylase (PDC) added in the form of Candida utilis cells showed higher stability towards benzaldehyde and temperature in comparison with partially purified p...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-0063-1

    authors: Satianegara G,Breuer M,Hauer B,Rogers PL,Rosche B

    更新日期:2006-03-01 00:00:00

  • Aerobic and oxygen-limited naphthalene-amended enrichments induced the dominance of Pseudomonas spp. from a groundwater bacterial biofilm.

    abstract::In this study, we aimed at determining the impact of naphthalene and different oxygen levels on a biofilm bacterial community originated from a petroleum hydrocarbon-contaminated groundwater. By using cultivation-dependent and cultivation-independent approaches, the enrichment, identification, and isolation of aerobic...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10668-y

    authors: Benedek T,Szentgyörgyi F,Szabó I,Farkas M,Duran R,Kriszt B,Táncsics A

    更新日期:2020-07-01 00:00:00

  • Effect of the osmotic conditions during sporulation on the subsequent resistance of bacterial spores.

    abstract::The causes of Bacillus spore resistance remain unclear. Many structures including a highly compact envelope, low hydration of the protoplast, high concentrations of Ca-chelated dipicolinic acid, and the presence of small acid-soluble spore proteins seem to contribute to resistance. To evaluate the role of internal pro...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-008-1519-x

    authors: Nguyen Thi Minh H,Guyot S,Perrier-Cornet JM,Gervais P

    更新日期:2008-08-01 00:00:00

  • A novel method for RNA extraction from Andosols using casein and its application to amoA gene expression study in soil.

    abstract::The lack of a universal method to extract RNA from soil hinders the progress of studies related to nitrification in soil, which is an important step in the nitrogen cycle. It is particularly difficult to extract RNA from certain types of soils such as Andosols (volcanic ash soils), which is the dominant agricultural s...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-4342-3

    authors: Wang Y,Nagaoka K,Hayatsu M,Sakai Y,Tago K,Asakawa S,Fujii T

    更新日期:2012-11-01 00:00:00

  • Soil acidification amendments change the rhizosphere bacterial community of tobacco in a bacterial wilt affected field.

    abstract::Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9347-0

    authors: Shen G,Zhang S,Liu X,Jiang Q,Ding W

    更新日期:2018-11-01 00:00:00

  • Fluorescence-based high-throughput screening system for R-ω-transaminase engineering and its substrate scope extension.

    abstract::ω-Transaminase (ω-TA) is an attractive alternative to metal catalysts for the stereoselective amination of prochiral ketones. The narrow substrate scope of an R-ω-transaminase from Mycobacterium vanbaalenii (MvTA) limits its application in R-amine synthesis. A fluorescence-based TA activity screening system was develo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10444-y

    authors: Cheng F,Chen XL,Xiang C,Liu ZQ,Wang YJ,Zheng YG

    更新日期:2020-04-01 00:00:00

  • Kimchi microflora: history, current status, and perspectives for industrial kimchi production.

    abstract::Kimchi, a traditional Korean food made by the fermentation of vegetables, has become popular globally because of its organoleptic, beneficial, and nutritional properties. Spontaneous kimchi fermentation in unsterilized raw materials leads to the growth of various lactic acid bacteria (LAB), which results in variations...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-014-5513-1

    authors: Jung JY,Lee SH,Jeon CO

    更新日期:2014-03-01 00:00:00

  • Lactobacillus reuteri CRL 1101 highly produces mannitol from sugarcane molasses as carbon source.

    abstract::Mannitol is a natural polyol extensively used in the food industry as low-calorie sugar being applicable for diabetic food products. We aimed to evaluate mannitol production by Lactobacillus reuteri CRL 1101 using sugarcane molasses as low-cost energy source. Mannitol formation was studied in free-pH batch cultures us...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-3945-z

    authors: Ortiz ME,Fornaguera MJ,Raya RR,Mozzi F

    更新日期:2012-08-01 00:00:00

  • Hybrid thermochemical processing: fermentation of pyrolysis-derived bio-oil.

    abstract::Thermochemical processing of biomass by fast pyrolysis provides a nonenzymatic route for depolymerization of biomass into sugars that can be used for the biological production of fuels and chemicals. Fermentative utilization of this bio-oil faces two formidable challenges. First is the fact that most bio-oil-associate...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-011-3495-9

    authors: Jarboe LR,Wen Z,Choi D,Brown RC

    更新日期:2011-09-01 00:00:00

  • Diversity, abundance, and spatial distribution of riverine microbial communities response to effluents from swine farm versus farmhouse restaurant.

    abstract::The 454 pyrosequencing technique was applied to evaluate microbial community composition in sediment and water samples collected from the river receiving effluents from a swine farm and a farmhouse restaurant, respectively. For each sample, 4,600 effective sequences were selected and used to do the bacterial diversity...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5772-x

    authors: Lu XM,Lu PZ

    更新日期:2014-09-01 00:00:00

  • Growth and differentiation of permanent and secondary mouse myogenic cell lines on microcarriers.

    abstract::Myogenesis involves the determination of progenitor cells to myoblasts, their fusion to yield multinuclear myotubes, and the maturation of myotubes to muscle fibres. This development is reflected in a time pattern of gene expression, e.g. of genes coding for desmin, the myogenic factors myogenin and myoD, the acetylch...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530100595

    authors: Bardouille C,Lehmann J,Heimann P,Jockusch H

    更新日期:2001-05-01 00:00:00