Identification and characterization of Δ12 and Δ12/Δ15 bifunctional fatty acid desaturases in the oleaginous yeast Lipomyces starkeyi.

Abstract:

:Fatty acid desaturases play vital roles in the synthesis of unsaturated fatty acids. In this study, Δ12 and Δ12/Δ15 fatty acid desaturases of the oleaginous yeast Lipomyces starkeyi, termed LsFad2 and LsFad3, respectively, were identified and characterized. Saccharomyces cerevisiae expressing LsFAD2 converted oleic acid (C18:1) to linoleic acid (C18:2), while a strain of LsFAD3-expressing S. cerevisiae converted oleic acid to linoleic acid, and linoleic acid to α-linolenic acid (C18:3), indicating that LsFad2 and LsFad3 were Δ12 and bifunctional Δ12/Δ15 fatty acid desaturases, respectively. The overexpression of LsFAD2 in L. starkeyi caused an accumulation of linoleic acid and a reduction in oleic acid levels. In contrast, overexpression of LsFAD3 induced the production of α-linolenic acid. Deletion of LsFAD2 and LsFAD3 induced the accumulation of oleic acid and linoleic acid, respectively. Our findings are significant for the commercial production of polyunsaturated fatty acids, such as ω-3 polyunsaturated fatty acids, in L. starkeyi.

authors

Matsuzawa T,Maehara T,Kamisaka Y,Ara S,Takaku H,Yaoi K

doi

10.1007/s00253-018-9345-2

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

8817-8826

issue

20

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-018-9345-2

journal_volume

102

pub_type

杂志文章
  • Bivariate genome-wide association study of the growth plasticity of Staphylococcus aureus in coculture with Escherichia coli.

    abstract::Phenotypic plasticity is the capacity to change the phenotype in response to different environments without alteration of the genotype. Despite sufficient evidence that microorganisms have a major role in the fitness and sickness of eukaryotes, there has been little research regarding microbial phenotypic plasticity. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10636-6

    authors: Zheng X,Bai J,Ye M,Liu Y,Jin Y,He X

    更新日期:2020-06-01 00:00:00

  • Effect of the osmotic conditions during sporulation on the subsequent resistance of bacterial spores.

    abstract::The causes of Bacillus spore resistance remain unclear. Many structures including a highly compact envelope, low hydration of the protoplast, high concentrations of Ca-chelated dipicolinic acid, and the presence of small acid-soluble spore proteins seem to contribute to resistance. To evaluate the role of internal pro...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-008-1519-x

    authors: Nguyen Thi Minh H,Guyot S,Perrier-Cornet JM,Gervais P

    更新日期:2008-08-01 00:00:00

  • Production of edible mushrooms in forests: trends in development of a mycosilviculture.

    abstract::Developing the production of ectomycorrhizal (ECM) mushrooms in forest has become a challenge. Only a few ECM species are currently cultivable. Controlled mycorrhization practices offer promising advance to produce currently uncultivable ECM mushrooms. The persistence of the production of edible species, either cultiv...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-010-3022-4

    authors: Savoie JM,Largeteau ML

    更新日期:2011-02-01 00:00:00

  • Preparation of the inactivated Newcastle disease vaccine by plasma activated water and evaluation of its protection efficacy.

    abstract::Vaccination has been regarded as the most effective way to reduce death and morbidity caused by infectious diseases in the livestock industry. In this study, plasma activated water (PAW) was introduced to prepare the inactivated Newcastle disease vaccine. Humoral immune response was tested by hemagglutination inhibiti...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-10106-8

    authors: Hongzhuan Z,Ying T,Xia S,Jinsong G,Zhenhua Z,Beiyu J,Yanyan C,Lulu L,Jue Z,Bing Y,Jing F

    更新日期:2020-01-01 00:00:00

  • A novel polygalacturonic acid bioflocculant REA-11 produced by Corynebacterium glutamicum: a proposed biosynthetic pathway and experimental confirmation.

    abstract::Corynebacterium glutamicum CCTCC M201005 produces a novel polygalacturonic acid bioflocculant, REA-11, consisting of galacturonic acid as the main structural unit. A biosynthetic pathway of REA-11 in C. glutamicum CCTCC M201005 was proposed. Evidence for the biosynthetic pathway was provided by: (1) analyzing the resp...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-003-1365-9

    authors: Li Y,He N,Guan H,Du G,Chen J

    更新日期:2003-12-01 00:00:00

  • Development of a low-cost sterilization biological indicator using Bacillus atrophaeus by solid-state fermentation.

    abstract::The production of biological indicators involving bacterial sporulation and multi-step downstream processes has been described. The goal of the present work was to use fermented material as the final product in a biological indicator, thereby reducing processing steps and costs. The performance of three different inex...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3491-0

    authors: Sella SR,Guizelini BP,Zanello PH,Vandenberghe LP,Ribeiro CA,Minozzo JC,Soccol CR

    更新日期:2012-01-01 00:00:00

  • Highly regio- and enantioselective synthesis of chiral intermediate for pregabalin using one-pot bienzymatic cascade of nitrilase and amidase.

    abstract::Nitrilase-mediated hydrolysis of isobutylsuccinonitrile (IBSN) is a highly attractive approach for (S)-3-cyano-5-methylhexanoic acid ((S)-CMHA), the critical chiral intermediate of pregabalin. In this study, a robust nitrilase from Arabis alpina (AaNIT) was screened and engineered. The N258D mutant was obtained with h...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-09857-1

    authors: Zhang Q,Wu ZM,Hao CL,Tang XL,Zheng RC,Zheng YG

    更新日期:2019-07-01 00:00:00

  • Fluorescence-based high-throughput screening system for R-ω-transaminase engineering and its substrate scope extension.

    abstract::ω-Transaminase (ω-TA) is an attractive alternative to metal catalysts for the stereoselective amination of prochiral ketones. The narrow substrate scope of an R-ω-transaminase from Mycobacterium vanbaalenii (MvTA) limits its application in R-amine synthesis. A fluorescence-based TA activity screening system was develo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10444-y

    authors: Cheng F,Chen XL,Xiang C,Liu ZQ,Wang YJ,Zheng YG

    更新日期:2020-04-01 00:00:00

  • Maximal release of highly bifidogenic soluble dietary fibers from industrial potato pulp by minimal enzymatic treatment.

    abstract::Potato pulp is a poorly utilized, high-volume co-processing product resulting from industrial potato starch manufacturing. Potato pulp mainly consists of the tuber plant cell wall material and is particularly rich in pectin, notably galactan branched rhamnogalacturonan I type pectin which has previously been shown to ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3092-y

    authors: Thomassen LV,Vigsnæs LK,Licht TR,Mikkelsen JD,Meyer AS

    更新日期:2011-05-01 00:00:00

  • Investigation of antimicrobial physiology of orthorhombic and monoclinic nanoallotropes of sulfur at the interface of transcriptome and metabolome.

    abstract::Nanosized elemental sulfur (ES) is already reported to exert superior antimicrobial efficacy than micron-sized ES, which encourages their use in drugs and therapeutics. The aim of the present study is to explore the possible route and mode of antimicrobial action of orthorhombic (α-SNPs) and monoclinic (β-SNPs) allotr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-4789-x

    authors: Roy Choudhury S,Mandal A,Ghosh M,Basu S,Chakravorty D,Goswami A

    更新日期:2013-07-01 00:00:00

  • Microbial communities in large-scale wood piles and their effects on wood quality and the environment.

    abstract::The demand of renewable energy sources, i.e. biomass, is steadily increasing worldwide to reduce the need of fossil energy sources. Biomass such as energy crops, woody species, forestry and agricultural residues are the most common renewable energy sources. Due to uneven demand for wood fuel, the material is mostly st...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-012-4164-3

    authors: Noll M,Jirjis R

    更新日期:2012-08-01 00:00:00

  • New insights on the colonization of the human gut by health-promoting bacteria.

    abstract::We are beginning to see how the microbiota of the human gastrointestinal tract (GIT) can drive the development of new products to benefit human health and wellbeing. Despite the growing market for prebiotics and probiotics, there are currently no commercial products available that aid or increase the attachment of hea...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-019-10336-w

    authors: Morrin ST,Hickey RM

    更新日期:2020-02-01 00:00:00

  • Efficient transient expression of human GM-CSF protein in Nicotiana benthamiana using potato virus X vector.

    abstract::The human granulocyte macrophage colony-stimulating factor (GM-CSF) is a glycoprotein with important clinical applications for the treatment of neutropenia and aplastic anemia and reducing infections associated with bone marrow transplants. We evaluated the potential for using a potato virus X (PVX) viral vector syste...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-0305-2

    authors: Zhou F,Wang ML,Albert HH,Moore PH,Zhu YJ

    更新日期:2006-10-01 00:00:00

  • Design of a core-shell type immuno-magnetic separation system and multiplex PCR for rapid detection of pathogens from food samples.

    abstract::We report an immuno-magnetic separation system developed by the immobilization of pathogen-specific antibodies on the core-shell magnetic beads. The magnetic beads were grafted with glycidylmethacrylate (GMA) using surface-initiated atom transfer radical polymerization (SI-ATRP). For immuno-magnetic separation (IMS) o...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5231-0

    authors: Ozalp VC,Bayramoglu G,Arica MY,Oktem HA

    更新日期:2013-11-01 00:00:00

  • Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module.

    abstract::A novel cell surface display system in Aspergillus oryzae was established by using a chitin-binding module (CBM) from Saccharomyces cerevisiae as an anchor protein. CBM was fused to the N or C terminus of green fluorescent protein (GFP) and the fusion proteins (GFP-CBM and CBM-GFP) were expressed using A. oryzae as a ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-010-2664-6

    authors: Tabuchi S,Ito J,Adachi T,Ishida H,Hata Y,Okazaki F,Tanaka T,Ogino C,Kondo A

    更新日期:2010-08-01 00:00:00

  • Effect of nutrient and selective inhibitor amendments on methane oxidation, nitrous oxide production, and key gene presence and expression in landfill cover soils: characterization of the role of methanotrophs, nitrifiers, and denitrifiers.

    abstract::Methane and nitrous oxide are both potent greenhouse gasses, with global warming potentials approximately 25 and 298 times that of carbon dioxide. A matrix of soil microcosms was constructed with landfill cover soils collected from the King Highway Landfill in Kalamazoo, Michigan and exposed to geochemical parameters ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-009-2238-7

    authors: Lee SW,Im J,Dispirito AA,Bodrossy L,Barcelona MJ,Semrau JD

    更新日期:2009-11-01 00:00:00

  • Substrate selectivity of various lipases in the esterification of cis- and trans-9-octadecenoic acid.

    abstract::The substrate selectivity of numerous commercially available lipases from microorganisms, plants and animal tissue towards 9-octadecenoic acids with respect to the cis/trans configuration of the C=C double bond was examined by the esterification of cis- and trans-9-octadecanoic acid (oleic and elaidic acid respectivel...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530051420

    authors: Borgdorf R,Warwel S

    更新日期:1999-04-01 00:00:00

  • Escherichia coli bioreporters for the detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene.

    abstract::The primary explosive found in most land mines, 2,4,6-trinitrotoluene (2,4,6-TNT), is often accompanied by 2,4-dinitrotoluene (2,4-DNT) and 1,3-dinitrobenzene (1,3-DNB) impurities. The latter two compounds, being more volatile, have been reported to slowly leak through land mine covers and permeate the soil under whic...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-4888-8

    authors: Yagur-Kroll S,Lalush C,Rosen R,Bachar N,Moskovitz Y,Belkin S

    更新日期:2014-01-01 00:00:00

  • Internal loop photobiodegradation reactor (ILPBR) for accelerated degradation of sulfamethoxazole (SMX).

    abstract::The internal loop photobiodegradation reactor (ILPBR) was evaluated for the degradation of the pharmaceutical sulfamethoxazole (SMX) using batch experiments following three protocols: photolysis alone (P), biodegradation alone (B), and intimately coupled photolysis and biodegradation (P&B). SMX was removed more rapidl...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3742-0

    authors: Yan N,Xia S,Xu L,Zhu J,Zhang Y,Rittmann BE

    更新日期:2012-04-01 00:00:00

  • Cellular fatty acid profile and H(+)-ATPase activity to assess acid tolerance of Bacillus sp. for potential probiotic functional attributes.

    abstract::The present study has been focused widely on comparative account of probiotic qualities of Bacillus spp. for safer usage. Initially, 170 heat resistant flora were isolated and selected for non-pathogenic cultures devoid of cytK, hblD, and nhe1 virulence genes. Subsequently, through biochemical tests along with 16S rRN...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5981-3

    authors: Shobharani P,Halami PM

    更新日期:2014-11-01 00:00:00

  • Enhancement of a pentacyclic tyrosine kinase inhibitor production in Cladosporium cf. cladosporioides by Cladosporol.

    abstract::The binaphthyl derivative cladosporol 3 was supplied from 60 to 200 mg l(-1) to shaken cultures of Cladosporium cf. cladosporioides. Compared to blank, fungal biomass was not affected by adding cladosporol till 100 mg l(-1): it rather increased at higher ratios between 150 and 200 mg l(-1). The production of the major...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-0054-2

    authors: Assante G,Bava A,Nasini G

    更新日期:2006-02-01 00:00:00

  • Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae.

    abstract::We used biodegradable plastics as fermentation substrates for the filamentous fungus Aspergillus oryzae. This fungus could grow under culture conditions that contained emulsified poly-(butylene succinate) (PBS) and emulsified poly-(butylene succinate-co-adipate) (PBSA) as the sole carbon source, and could digest PBS a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-004-1853-6

    authors: Maeda H,Yamagata Y,Abe K,Hasegawa F,Machida M,Ishioka R,Gomi K,Nakajima T

    更新日期:2005-06-01 00:00:00

  • Kinetics of biodegradation of mixtures of polycyclic aromatic hydrocarbons.

    abstract::The kinetics of biodegradation of polycyclic aromatic hydrocarbons (PAHs) by a mixed culture were determined in a creosote-contaminated soil and in a pristine soil. A competitive-inhibition model was able to represent the kinetics of degradation of PAHs from the creosote-contaminated soil, from the lag phase through t...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-002-1104-7

    authors: Lotfabad SK,Gray MR

    更新日期:2002-11-01 00:00:00

  • Biosynthesis of pyruvic acid from glucose by Blastobotrys adeninivorans.

    abstract::The ability of taxonomically different yeasts to synthesize pyruvic acid (PA) from glucose was studied. The study showed that many yeasts are able to produce PA from glucose under the condition of growth limitation by thiamine. This ability was found in the yeast Blastobotrys adeninivorans for the first time. The prod...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7618-1

    authors: Kamzolova SV,Morgunov IG

    更新日期:2016-09-01 00:00:00

  • The role of N1 domain on the activity, stability, substrate specificity and raw starch binding of amylopullulanase of the extreme thermophile Geobacillus thermoleovorans.

    abstract::In order to understand the role of N1 domain (1-257 aa) in the amylopullulanase (gt-apu) of the extremely thermophilic bacterium Geobacillus thermoleovorans NP33, N1 deletion construct (gt-apuΔN) has been generated and expressed in Escherichia coli. The truncated amylopullulanase (gt-apuΔN) exhibits similar pH and tem...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-6345-8

    authors: Nisha M,Satyanarayana T

    更新日期:2015-07-01 00:00:00

  • Characterization of four endophytic fungi as potential consolidated bioprocessing hosts for conversion of lignocellulose into advanced biofuels.

    abstract::Recently, several endophytic fungi have been demonstrated to produce volatile organic compounds (VOCs) with properties similar to fossil fuels, called "mycodiesel," while growing on lignocellulosic plant and agricultural residues. The fact that endophytes are plant symbionts suggests that some may be able to produce l...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8091-1

    authors: Wu W,Davis RW,Tran-Gyamfi MB,Kuo A,LaButti K,Mihaltcheva S,Hundley H,Chovatia M,Lindquist E,Barry K,Grigoriev IV,Henrissat B,Gladden JM

    更新日期:2017-03-01 00:00:00

  • Heterologous expression of human paraoxonases in Pseudomonas aeruginosa inhibits biofilm formation and decreases antibiotic resistance.

    abstract::Quorum sensing (QS) regulates virulence and biofilm formation in Pseudomonas aeruginosa and other medically relevant bacteria. Human paraoxonases (hPONs) are a family of closely related enzymes with multiple functions, including inactivation of the QS signal molecule in P. aeruginosa. However, there is no direct evide...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-009-1890-2

    authors: Ma F,Wang Y,Zhang Y,Xiong N,Yang B,Chen S

    更新日期:2009-05-01 00:00:00

  • Efficient induction of ginsenoside biosynthesis and alteration of ginsenoside heterogeneity in cell cultures of Panax notoginseng by using chemically synthesized 2-hydroxyethyl jasmonate.

    abstract::Chemically synthesized 2-hydroxyethyl jasmonate (HEJA) was for the first time employed to induce the ginsenoside biosynthesis and to manipulate the product heterogeneity in plant cell cultures. The dose response and timing of HEJA elicitation were investigated in cell suspension cultures of Panax notoginseng. The opti...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-0089-4

    authors: Wang W,Zhao ZJ,Xu Y,Qian X,Zhong JJ

    更新日期:2006-04-01 00:00:00

  • Biochemical and physicochemical processes contributing to the removal of endocrine-disrupting chemicals and pharmaceuticals by the aquatic ascomycete Phoma sp. UHH 5-1-03.

    abstract::The environmentally widespread micropollutants bisphenol A (BPA), carbamazepine (CBZ), 17α-ethinylestradiol (EE2), diclofenac (DF), sulfamethoxazole (SMX), technical nonylphenol (t-NP) and triclosan (TCS) were used to assess the potential of the laccase-producing freshwater ascomycete Phoma sp. strain UHH 5-1-03 for m...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-7113-0

    authors: Hofmann U,Schlosser D

    更新日期:2016-03-01 00:00:00

  • Flavin reductase coupling with two monooxygenases involved in dibenzothiophene desulfurization: purification and characterization from a non-desulfurizing bacterium, Paenibacillus polymyxa A-1.

    abstract::The dibenzothiophene (DBT) desulfurizing bacterium metabolizes DBT to form 2-hydroxybiphenyl without breaking the carbon skeleton. Of the DBT desulfurization enzymes, DszC and DszA catalyze monooxygenation reactions, both requiring flavin reductase. We searched for non-DBT-desulfurizing microorganisms producing a flav...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-002-1082-9

    authors: Ohshiro T,Aoi Y,Torii K,Izumi Y

    更新日期:2002-09-01 00:00:00