Biotechnological production of L-ribose from L-arabinose.


:L-Ribose is a rare and expensive sugar that can be used as a precursor for the production of L-nucleoside analogues, which are used as antiviral drugs. In this work, we describe a novel way of producing L-ribose from the readily available raw material L-arabinose. This was achieved by introducing L-ribose isomerase activity into L-ribulokinase-deficient Escherichia coli UP1110 and Lactobacillus plantarum BPT197 strains. The process for L-ribose production by resting cells was investigated. The initial L-ribose production rates at 39 degrees C and pH 8 were 0.46 +/- 0.01 g g(-1) h(-1) (1.84 +/- 0.03 g l(-1) h(-1)) and 0.27 +/- 0.01 g g(-1) h(-1) (1.91 +/- 0.1 g l(-1) h(-1)) for E. coli and for L. plantarum, respectively. Conversions were around 20% at their highest in the experiments. Also partially purified protein precipitates having both L-arabinose isomerase and L-ribose isomerase activity were successfully used for converting L-arabinose to L-ribose.


Helanto M,Kiviharju K,Granström T,Leisola M,Nyyssölä A




Has Abstract


2009-05-01 00:00:00












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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Chavaroche AA,Springer J,Kooy F,Boeriu C,Eggink G

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Sosedov O,Stolz A

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    pub_type: 杂志文章,评审


    authors: Gaytán I,Burelo M,Loza-Tavera H

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    pub_type: 杂志文章,评审


    authors: McMullan G,Meehan C,Conneely A,Kirby N,Robinson T,Nigam P,Banat IM,Marchant R,Smyth WF

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    authors: Khosravi-Darani K,Mokhtari ZB,Amai T,Tanaka K

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    authors: Hou X,Yao S

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    authors: Wang XC,Shen JM,Chen ZL,Zhao X,Xu H

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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章,评审


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    authors: Maranga L,Rueda P,Antonis AF,Vela C,Langeveld JP,Casal JI,Carrondo MJ

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    authors: Tresse O,Lorrain MJ,Rho D

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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章


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