Biodegradation and kinetic analysis of phthalates by an Arthrobacter strain isolated from constructed wetland soil.

abstract：:A cytochrome P450 and an iron-sulfur protein, whose expression was specifically induced during growth of Mycobacterium sp. strain HE5 on morpholine as the sole source of carbon, nitrogen, and energy were purified to apparent homogeneity. Due to the lack of enzymatic activity, carbon monoxide difference spectra and det...

journal_title：Applied microbiology and biotechnology

authors： Sielaff B,Andreesen JR,Schräder T

更新日期：2001-08-01 00:00:00

abstract：:Blowfly (Diptera: Calliphoridae) species Lucilia sericata (Meigen) and related species Lucilia cuprina (Wiedmann) are important agricultural pests, assist in forensic fields and also have a therapeutic role in medicine. Both species (though predominantly L. sericata) are utilised in a clinical setting for maggot debri...

journal_title：Applied microbiology and biotechnology

authors： Gasz NE,Geary MJ,Doggett SL,Harvey ML

更新日期：2021-01-07 00:00:00

abstract：:Understanding the effect of conserved domains reconstruction of alginate lyases on action mode is essential for their application and in-depth study. We report the expression and action mode of recombinant alginate lyase (AlyM) and its conserved domain reconstruction forms (AlyMΔCBM, cAlyM, and AlyMΔ58C). The enzymati...

journal_title：Applied microbiology and biotechnology

authors： Yang M,Li N,Yang S,Yu Y,Han Z,Li L,Mou H

更新日期：2019-01-01 00:00:00

abstract：:Microbes engineered to display heterologous proteins could be useful biotechnological tools for protein engineering, lignocellulose degradation, biocatalysis, bioremediation, and biosensing. Bacillus subtilis is a promising host to display proteins, as this model Gram-positive bacterium is genetically tractable and al...

journal_title：Applied microbiology and biotechnology

authors： Huang GL,Gosschalk JE,Kim YS,Ogorzalek Loo RR,Clubb RT

更新日期：2018-08-01 00:00:00

abstract：:The anaerobic degradation of phenol was studied in a fed-batch culture. Nitrate was added as electron acceptor and phenol was provided three times, to a final concentration of 200 mg/l. Randomly amplified polymorphic DNA (RAPD) and terminal fraction fragment length polymorphism (T-RFLP) were used and compared in order...

journal_title：Applied microbiology and biotechnology

authors： Guieysse B,Wickström P,Forsman M,Mattiasson B

更新日期：2001-09-01 00:00:00

abstract：:Although host proteases are often considered to have a negative impact upon heterologous protein production by filamentous fungi, relatively little is known about the pattern of their appearance in recombinant fungal bioprocesses. In the present study, we investigated extracellular proteases from a filamentous fungus,...

journal_title：Applied microbiology and biotechnology

authors： Li Q,Harvey LM,McNeil B

更新日期：2008-02-01 00:00:00

abstract：:Histone lysine acetylation orchestrates transcriptional activity essential for diverse cellular events across organisms, but it remains poorly understood how an acetylated lysine affects cellular functions in filamentous fungal pathogens. Here, we show the functions of a histone acetyltransferase that is phylogenetica...

journal_title：Applied microbiology and biotechnology

authors： Wang JJ,Cai Q,Qiu L,Ying SH,Feng MG

更新日期：2018-02-01 00:00:00

abstract：:Cultures of Umbelopsis ramanniana (=Mucor ramannianus) were grown in fluid Sabouraud medium for 3 days, dosed with 0.23 mM benzo[f]quinoline, benzo[h]quinoline, or phenanthridine (benzo[c]quinoline), and incubated for another 18 days. Cultures were extracted and metabolites (66-75% of the UV absorbance) were separated...

journal_title：Applied microbiology and biotechnology

authors： Sutherland JB,Cross EL,Heinze TM,Freeman JP,Moody JD

更新日期：2005-05-01 00:00:00

abstract：:Pichia pastoris is widely used as a host system for heterologous protein expression in both academia and industry. Production is typically accomplished by a fed-batch induction process that is known to have negative impacts on cell physiology that impose limits on both protein yields and quality. We have analysed reco...

journal_title：Applied microbiology and biotechnology

authors： Hesketh AR,Castrillo JI,Sawyer T,Archer DB,Oliver SG

更新日期：2013-11-01 00:00:00

abstract：:Significant resources are invested into efforts to improve the production yields of natural products from Actinobacteria, a well-recognized source of leads for several industries, most notably pharmaceutical one. Introduction of changes into genes for ribosomal protein S12 (rpsL) and/or 16S rRNA methylation (rsmG) is ...

journal_title：Applied microbiology and biotechnology

authors： Lopatniuk M,Myronovskyi M,Nottebrock A,Busche T,Kalinowski J,Ostash B,Fedorenko V,Luzhetskyy A

更新日期：2019-09-01 00:00:00

abstract：:Among the Streptomyces species, Streptomyces lividans has often been used for the production of heterologous proteins as it can secrete target proteins directly into the culture medium. Streptomyces rimosus, on the other hand, has for long been used at an industrial scale for oxytetracycline production, and it holds '...

journal_title：Applied microbiology and biotechnology

authors： Carrillo Rincón AF,Magdevska V,Kranjc L,Fujs Š,Müller R,Petković H

更新日期：2018-03-01 00:00:00

abstract：:Rhodococcus erythropolis N9T-4, which is an extremely oligotrophic bacterium, can survive in a completely inorganic medium with no additional carbon source. This bacterium utilizes atmospheric CO2, but does not require any additional energy source such as light and hydrogen gas, required by autotrophic microorganisms....

journal_title：Applied microbiology and biotechnology

authors： Matsuoka T,Yoshida N

更新日期：2019-05-01 00:00:00

abstract：:Escherichia coli K-12 was cultured under anaerobic conditions to form biofilm on carbon fiber electrodes in glucose-containing medium. The anodic current increased with the development of the biofilm and depended on the glucose concentration. Cyclic voltammetric results support the presence of a redox compound(s) excr...

journal_title：Applied microbiology and biotechnology

authors： Wang YF,Tsujimura S,Cheng SS,Kano K

更新日期：2007-10-01 00:00:00

abstract：:In light of emerging antibiotic resistance, bacterial cell wall lytic enzymes are promising antimicrobial agents that degrade bacterial peptidoglycan while specifically recognizing the target bacterium. The efficacy of lytic enzymes against several multi-drug-resistant pathogens infecting humans has led to many effort...

journal_title：Applied microbiology and biotechnology

authors： Bhagwat A,Mixon M,Collins CH,Dordick JS

更新日期：2020-11-01 00:00:00

abstract：:Microbial cell factories offer an economic approach for synthesizing "natural'" aromatic flavor compounds. During their fermentation process, the inefficient synthesis pathway and product cytotoxicity are the major barriers to the high-level production. This study combined metabolic engineering and tolerance engineeri...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Zhang Z,Lu X,Zong H,Zhuge B

更新日期：2020-12-01 00:00:00

abstract：:The Vitreoscilla hemoglobin (VHb) gene (vgb) was integrated into the chromosome of Bacillus thuringiensis BMB171 using integrative vector pEG491. The production of VHb was confirmed by CO-difference spectra analysis. Fermentation experiments results showed that with the production of VHb, the critical oxygen concentra...

journal_title：Applied microbiology and biotechnology

authors： Liang F,Shouwen C,Ming S,Ziniu Y

更新日期：2007-02-01 00:00:00

abstract：:The secreted proteome of Pichia pastoris X-33 was investigated in methanol-induced cultures with a goal to enhance the secretion and purification of recombinant proteins. In a fed-batch fermentation at 30 °C, more host proteins were found in greater concentrations compared to cultures grown at 25 °C. Protein samples c...

journal_title：Applied microbiology and biotechnology

authors： Huang CJ,Damasceno LM,Anderson KA,Zhang S,Old LJ,Batt CA

更新日期：2011-04-01 00:00:00

abstract：:Analysis of gene expression data related to assimilation and biosynthesis of nitrogen-containing compounds amino acids, peptides, and nucleotides was used to monitor availability of these nutrients to Escherichia coli MG1655 growing on Luria-Bertani medium. The data indicate that free amino acids and nucleotides only ...

journal_title：Applied microbiology and biotechnology

authors： Baev MV,Baev D,Radek AJ,Campbell JW

更新日期：2006-07-01 00:00:00

abstract：:The environmentally widespread micropollutants bisphenol A (BPA), carbamazepine (CBZ), 17α-ethinylestradiol (EE2), diclofenac (DF), sulfamethoxazole (SMX), technical nonylphenol (t-NP) and triclosan (TCS) were used to assess the potential of the laccase-producing freshwater ascomycete Phoma sp. strain UHH 5-1-03 for m...

journal_title：Applied microbiology and biotechnology

authors： Hofmann U,Schlosser D

更新日期：2016-03-01 00:00:00

abstract：:One solution to the global crisis of antibiotic resistance is the discovery of novel antimicrobial compounds for clinical application. Marine organisms are an attractive and, as yet, relatively untapped resource of new natural products. Cell extracts from the marine diatom, Phaeodactylum tricornutum, have antibacteria...

journal_title：Applied microbiology and biotechnology

authors： Desbois AP,Lebl T,Yan L,Smith VJ

更新日期：2008-12-01 00:00:00

abstract：:Production of gluconic acid by cells of Aspergillus niger immobilized on polyurethane foam was studied in repeated-batch shake-flask and bubble-column fermentations. For passive immobilization, various amounts of polyurethane foam and spore suspension were tested in order to obtain a suitable combination for optimal c...

journal_title：Applied microbiology and biotechnology

authors： Vassilev NB,Vassileva MC,Spassova DI

更新日期：1993-06-01 00:00:00

abstract：:The redox-sensing transcriptional repressor Rex (Rex) displayed diverse functions in different microbial species. Nowadays, only part function of rex has been verified in vitro and alcohol dehydrogenase gene (adhE) as the target of Rex has been widely reported. In this study, rex was knocked out in Thermoanaerobacteri...

journal_title：Applied microbiology and biotechnology

authors： Qu C,Chen L,Li Y,Fu H,Wang J

更新日期：2020-06-01 00:00:00

abstract：:Scenedesmus is a genus of microalgae employed for several industrial uses. Industrial cultivations are performed in open ponds or in closed photobioreactors (PBRs). In the last years, a novel type of PBR based on immobilized microalgae has been developed termed porous substrate photobioreactors (PSBR) to achieve signi...

journal_title：Applied microbiology and biotechnology

authors： Carbone DA,Olivieri G,Pollio A,Gabriele,Melkonian M

更新日期：2017-12-01 00:00:00

abstract：:In an effort to produce ricinoleic acid (RA), an important natural raw material with great values as a petrochemical replacement, in Schizosaccharomyces pombe, we introduced Claviceps purpurea oleate Δ12-hydroxylase gene (CpFAH12) to S. pombe, putting it under the control of an inducible nmt1 promoter. However, RA was...

journal_title：Applied microbiology and biotechnology

authors： Yazawa H,Holic R,Kumagai H,Uemura H

更新日期：2013-09-01 00:00:00

abstract：:Using a reverse-transcription polymerase chain reaction (RT-PCR) technique, cytochrome P450 genes were cloned from the lignin-degrading basidiomycete, Coriolus versicolor. One possible P450 gene was identified, which consisted of 1,672 nucleotides and a poly(A) tail and encoded a deduced protein containing 449 amino a...

journal_title：Applied microbiology and biotechnology

authors： Ichinose H,Wariishi H,Tanaka H

更新日期：2002-01-01 00:00:00

abstract：:Various techniques are used to adjust the flavors of foods and beverages to new market demands. Although synthetic flavoring chemicals are still widely used, flavors produced by biological methods (bioflavors) are now more and more requested by consumers, increasingly concerned with health and environmental problems c...

journal_title：Applied microbiology and biotechnology

authors： Vanderhaegen B,Neven H,Coghe S,Verstrepen KJ,Derdelinckx G,Verachtert H

更新日期：2003-08-01 00:00:00

abstract：:The purpose of this paper is to present biomass and nutrient uptake data from Neochloris oleoabundans production in an open trough system. The growth medium used was BG11, temperature ranged from 16.7 °C to 25.3 °C, and pH ranged from 5.52 to 9.94 because the customary pH increase during algal biomass production was m...

journal_title：Applied microbiology and biotechnology

authors： Murray KE,Healy FG,McCord RS,Shields JA

更新日期：2011-04-01 00:00:00

abstract：:The integration of physiological knowledge into process control strategies is a cornerstone for the improvement of biopharmaceutical cell culture technologies. The present contribution investigates the applicability of specific productivity as a physiological control parameter in a cell culture process producing a mon...

journal_title：Applied microbiology and biotechnology

authors： Zalai D,Hevér H,Lovász K,Molnár D,Wechselberger P,Hofer A,Párta L,Putics Á,Herwig C

更新日期：2016-08-01 00:00:00

abstract：:Hydroxy fatty acids (HFA) have gained importance because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. The bacterial isolate Pseudomonas aeruginosa (PR3) was reported to produce mono-, di-, and trihydroxy fatty acids from different unsaturated fatty ac...

journal_title：Applied microbiology and biotechnology

authors： Chang IA,Kim IH,Kang SC,Hou CT,Kim HR

更新日期：2007-02-01 00:00:00

abstract：:Batch assays are currently used to study the kinetic behavior of microbial growth. However, it has been shown that the outcome of batch experiments is greatly influenced by the initial ratio of substrate concentration (S(o)) to biomass concentration (Xo). Substrate-sufficient batch culture is known to have mechanisms ...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Chen GH,Rols JL

更新日期：1999-11-01 00:00:00