Opportunities for broadening the application of cell wall lytic enzymes.

Abstract:

:In light of emerging antibiotic resistance, bacterial cell wall lytic enzymes are promising antimicrobial agents that degrade bacterial peptidoglycan while specifically recognizing the target bacterium. The efficacy of lytic enzymes against several multi-drug-resistant pathogens infecting humans has led to many efforts focused on in vivo therapeutic applications. However, the potential for lytic enzymes to combat bacterial contamination in environments outside the human body is underexplored. The persistence of pathogenic bacteria, in either planktonic or biofilm states and on various surfaces, has facilitated the spread of bacterial infections, necessitating the development of robust strategies for detecting and killing resistant bacteria in diverse environments. Here, we present an overview of the current state-of-the-art of exploiting lytic enzymes for non-therapeutic applications including pathogen decontamination in social infrastructures and food decontamination, as well as pathogen detection. KEY POINTS: • Lytic enzymes are effective antimicrobial, antibiofilm, and sporicidal agents. • Pathogen detection using lytic enzyme-binding domains is rapid and highly sensitive. • Domain engineering is required for enhanced enzyme activity in complex environments.

authors

Bhagwat A,Mixon M,Collins CH,Dordick JS

doi

10.1007/s00253-020-10862-y

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

9019-9040

issue

21

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-020-10862-y

journal_volume

104

pub_type

杂志文章,评审
  • Characterization and flocculation mechanism of a bioflocculant from potato starch wastewater.

    abstract::This study investigated the characterization and flocculation mechanism of a bioflocculant prepared using potato starch wastewater. The optimal culture conditions of this strain were determined as 4 g K2HPO4, 2 g KH2PO4, 0.2 g MgSO4, 0.1 g NaCl, and 2.0 g urea dissolved in 1.0 L potato starch wastewater with no need o...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6589-y

    authors: Guo J,Lau AK,Zhang Y,Zhao J

    更新日期:2015-07-01 00:00:00

  • Recent research progress on microbial L-asparaginases.

    abstract::L-Asparaginases (EC 3.5.1.1) are enzymes that catalyze the hydrolysis of L-asparagine to L-aspartic acid and found in a variety of organisms from microorganisms to mammals. However, they are mainly expressed and produced by microorganisms. Microbial L-asparaginases have received sustained attention due to their irrepl...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-014-6271-9

    authors: Zuo S,Zhang T,Jiang B,Mu W

    更新日期:2015-02-01 00:00:00

  • Natural and engineered promoters for gene expression in Lactobacillus species.

    abstract::Lactobacillus species are attractive hosts for the expression of heterologous proteins, antigens, vaccines, and drugs due to their GRAS (generally recognized as safe) status. The bioengineering techniques open new possibilities of improving Lactobacillus strains. In this regard, the control of the gene expression in L...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-020-10426-0

    authors: Peirotén Á,Landete JM

    更新日期:2020-05-01 00:00:00

  • Perstraction of intracellular pigments by submerged cultivation of Monascus in nonionic surfactant micelle aqueous solution.

    abstract::"Milking processing" describes the cultivation of microalgae in a water-organic solvent two-phase system that consists of simultaneous fermentation and secretion of intracellular product. It is usually limited by the conflict between the biocompatibility of the organic solvent to the microorganisms and the ability of ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3851-9

    authors: Hu Z,Zhang X,Wu Z,Qi H,Wang Z

    更新日期:2012-04-01 00:00:00

  • Heterologous signal peptides-directing secretion of Streptomyces mobaraensis transglutaminase by Bacillus subtilis.

    abstract::Microbial transglutaminase (MTG) from Streptomyces mobaraensis has been widely used for crosslinking proteins in order to acquire products with improved properties. To improve the yield and enable a facile and efficient purification process, recombinant vectors, harboring various heterologous signal peptide-encoding f...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9000-y

    authors: Mu D,Lu J,Qiao M,Kuipers OP,Zhu J,Li X,Yang P,Zhao Y,Luo S,Wu X,Jiang S,Zheng Z

    更新日期:2018-07-01 00:00:00

  • Algae-induced taste and odour problems at low temperatures and the cold stress response hypothesis.

    abstract::The existence of taste and odour (T&O) in drinking water is one of the principal causes of consumer complaints and is commonly related to algae growth. Numerous studies have confirmed the existence of algal blooms emerging specifically in low-temperature periods, herein referred to as "cold algae"; these include chrys...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-020-10884-6

    authors: Cen C,Zhang K,Zhang T,Zhou X,Pan R

    更新日期:2020-11-01 00:00:00

  • Caecal environment of rats fed far East Asian-modelled diets.

    abstract::To clarify the effect of type of foods on the intestinal environment, Far East Asian- (FEA; rich in rice starch, soy protein and soy oil) and Far East Asian marine- (FEAM; rich in rice starch, fish meal, fish oil and brown alga) modelled diets and sucrose, casein and beef tallow-rich (SCB) diet were prepared. After th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5535-8

    authors: An C,Kuda T,Yazaki T,Takahashi H,Kimura B

    更新日期:2014-05-01 00:00:00

  • Proteomic understanding of intracellular responses of recombinant Chinese hamster ovary cells cultivated in serum-free medium supplemented with hydrolysates.

    abstract::In order to understand the intracellular responses in recombinant CHO (rCHO) cells producing antibody in serum-free medium (SFM) supplemented with optimized hydrolysates mixtures, yielding the highest specific growth rate (μ, SFM#S1) or the highest specific antibody productivity (q(Ab,) SFM#S2), differentially express...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3106-9

    authors: Kim JY,Kim YG,Han YK,Choi HS,Kim YH,Lee GM

    更新日期:2011-03-01 00:00:00

  • Substrate specificity and membrane topologies of the iron-containing ω3 and ω6 desaturases from Mortierella alpina.

    abstract::Polyunsaturated fatty acids (PUFAs) are essential lipids for cell function, normal growth, and development, serving as key structural components of biological membranes and modulating critical signal transduction events. Omega-3 (n-3) long chain PUFAs (LC-PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8585-x

    authors: Wang M,Chen H,Ailati A,Chen W,Chilton FH,Todd Lowther W,Chen YQ

    更新日期:2018-01-01 00:00:00

  • Safeguarding bacterial resources promotes biotechnological innovation.

    abstract::Environmental research delivers valuable bacterial resources for biotechnology. We believe that systematic long-term preservation of bacteria will promote future biotechnological innovations, by safeguarding the accessibility of bacteria already recognized to have interesting features and providing a "pool" of bacteri...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-011-3797-y

    authors: Heylen K,Hoefman S,Vekeman B,Peiren J,De Vos P

    更新日期:2012-05-01 00:00:00

  • Coupling ssDNA recombineering with CRISPR-Cas9 for Escherichia coli DnaG mutations.

    abstract::Homologous recombination-based recombineering is a widely used DNA cloning and modification technique; recombineering efficiency improvement would be helpful for high-throughput DNA manipulation. Escherichia coli primase DnaG variants, such as DnaG Q576A and DnaG K580A, increase the recombineering efficiency via impai...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-09744-9

    authors: Li J,Sun J,Gao X,Wu Z,Shang G

    更新日期:2019-04-01 00:00:00

  • Metabolic response against sulfur-containing heterocyclic compounds by the lignin-degrading basidiomycete Coriolus versicolor.

    abstract::The fungal conversions of sulfur-containing heterocyclic compounds were investigated using the lignin-degrading basidiomycete Coriolus versicolor. The fungus metabolized a series of sulfur compounds--25 structurally related thiophene derivatives--via several different pathways. Under primary metabolic conditions, C. v...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-001-0926-z

    authors: Ichinose H,Nakamizo M,Wariishi H,Tanaka H

    更新日期:2002-03-01 00:00:00

  • Analysis of acyl CoA ester intermediates of the mevalonate pathway in Saccharomyces cerevisiae.

    abstract::The mevalonate pathway plays an important role in providing the cell with a number of essential precursors for the synthesis of biomass constituents. With respect to their chemical structure, the metabolites of this pathway can be divided into two groups: acyl esters [acetoacetyl CoA, acetyl CoA, hydroxymethylglutaryl...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-004-1697-0

    authors: Seker T,Møller K,Nielsen J

    更新日期:2005-04-01 00:00:00

  • Efficient xylose utilization leads to highest lipid productivity in Candida tropicalis SY005 among six yeast strains grown in mixed sugar medium.

    abstract::Six local isolates of yeasts were screened for cell mass and lipid production in mixed glucose and xylose medium. Candida tropicalis SY005 and Trichosporon (Apiotrichum) loubieri SY006 showed significant lipid accumulation of 24.6% and 32% (dry cell weight), respectively when grown in medium containing equal mass of b...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10443-z

    authors: Chattopadhyay A,Maiti MK

    更新日期:2020-04-01 00:00:00

  • 1,3-Propanediol production in a two-step process fermentation from renewable feedstock.

    abstract::In this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>20...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3369-1

    authors: Mendes FS,González-Pajuelo M,Cordier H,François JM,Vasconcelos I

    更新日期:2011-11-01 00:00:00

  • Expression of Streptomyces genes encoding extracellular enzymes in Brevibacterium lactofermentum: secretion proceeds by removal of the same leader peptide as in Streptomyces lividans.

    abstract::The alpha-amylase gene (amy) from Streptomyces griseus IMRU 3570 and the beta-galactosidase gene (lac) from S. lividans were subcloned into Brevibacterium lactofermentum or B. lactofermentum/Escherichia coli shuttle vectors. The amy gene was not expressed in B. lactofermentum from its own promoter but was efficiently ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00170087

    authors: Cadenas RF,Gil JA,Martín JF

    更新日期:1992-12-01 00:00:00

  • Molecular cloning and comparative analysis of four beta-galactosidase genes from Bifidobacterium bifidum NCIMB41171.

    abstract::Bifidobacterium bifidum NCIMB41171 carries four genes encoding different beta-galactosidases. One of them, named bbgIII, consisted of an open reading frame of 1,935 amino acid (a.a.) residues encoding a protein with a multidomain structure, commonly identified on cell wall bound enzymes, having a signal peptide, a mem...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-1099-1

    authors: Goulas TK,Goulas AK,Tzortzis G,Gibson GR

    更新日期:2007-10-01 00:00:00

  • Identification and biochemical characterization of a novel cold-adapted 1,3-α-3,6-anhydro-L-galactosidase, Ahg786, from Gayadomonas joobiniege G7.

    abstract::Agar is a major polysaccharide of red algal cells and is mainly decomposed into neoagarobiose by the co-operative effort of β-agarases. Neoagarobiose is hydrolyzed into monomers, D-galactose and 3,6-anhydro-L-galactose, via a microbial oxidative process. Therefore, the enzyme, 1,3-α-3,6-anhydro-L-galactosidase (α-neoa...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9277-x

    authors: Asghar S,Lee CR,Park JS,Chi WJ,Kang DK,Hong SK

    更新日期:2018-10-01 00:00:00

  • A computer-controlled system to simulate conditions of the large intestine with peristaltic mixing, water absorption and absorption of fermentation products.

    abstract::This paper introduces a new type of system to simulate conditions in the large intestine. This system combines removal of metabolites and water with peristaltic mixing to obtain and handle physiological concentrations of microorganisms, dry matter and microbial metabolites. The system has been designed to be complemen...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530051622

    authors: Minekus M,Smeets-Peeters M,Bernalier A,Marol-Bonnin S,Havenaar R,Marteau P,Alric M,Fonty G,Huis in't Veld JH

    更新日期:1999-12-01 00:00:00

  • Characterization and regulation of the 2,3-butanediol pathway in Serratia marcescens.

    abstract::Serratia marcescens has been proved to be a potential strain for industrial 2,3-butanediol production for its high yield, productivity, and other advantages. In this study, the genes slaA, slaB, slaC, and slaR were successfully cloned which were further confirmed to be encoding acetolactate decarboxylase, acetolactate...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3608-5

    authors: Rao B,Zhang LY,Sun J,Su G,Wei D,Chu J,Zhu J,Shen Y

    更新日期:2012-03-01 00:00:00

  • Dialysis cultures.

    abstract::Dialysis techniques are discussed as a means for effective removal of low-molecular-mass components from fermentation broth to reach high cell density. Reactor systems and process strategies, the relevant properties of membranes and examples for high-density fermentation with dialysis, and problems related to scale-up...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530051312

    authors: Pörtner R,Märkl H

    更新日期:1998-10-01 00:00:00

  • Fermentation of protopanaxadiol type ginsenosides (PD) with probiotic Bifidobacterium lactis and Lactobacillus rhamnosus.

    abstract::Ginsenosides are believed to be the principal components behind the pharmacological actions of ginseng, and their bioactive properties are closely related to the type, position, and number of sugar moieties attached to the aglycone; thus, modification of the sugar chains may markedly change their biological activities...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8295-4

    authors: Tan JS,Yeo CR,Popovich DG

    更新日期:2017-07-01 00:00:00

  • Characterization of a negative regulator AveI for avermectin biosynthesis in Streptomyces avermitilis NRRL8165.

    abstract::A transcriptional activator for actinorhodin biosynthesis, AtrA, was previously characterized in Streptomyces coelicolor A3(2), and an orthologue of atrA, named aveI, is identified in the Streptomyces avermitilis NRRL8165 genome (Uguru et al., Mol Microbiol, 58:131-150, 2005). In this study, genetic and functional cha...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-008-1545-8

    authors: Chen L,Lu Y,Chen J,Zhang W,Shu D,Qin Z,Yang S,Jiang W

    更新日期:2008-08-01 00:00:00

  • The recA gene is crucial to mediate colonization of Bacillus cereus 905 on wheat roots.

    abstract::Bacillus cereus 905, one of the plant growth-promoting rhizobacteria (PGPRs), is capable of colonizing wheat roots in a large population size. From previous studies, we learned that the sodA2-encoding manganese-containing superoxide dismutase (MnSOD2) is important for B. cereus 905 to survive in wheat rhizosphere. In ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10915-2

    authors: Gao T,Ding M,Wang Q

    更新日期:2020-11-01 00:00:00

  • A promiscuous prenyltransferase from Aspergillus oryzae catalyses C-prenylations of hydroxynaphthalenes in the presence of different prenyl donors.

    abstract::Prenyltransferases of the dimethylallyltryptophan synthase (DMATS) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. Most members of this superfamily accepted as prenyl...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5509-x

    authors: Pockrandt D,Sack C,Kosiol T,Li SM

    更新日期:2014-06-01 00:00:00

  • Transcriptional changes involved in inhibition of biofilm formation by ε-polylysine in Salmonella Typhimurium.

    abstract::The pathogenicity of Salmonella Typhimurium, a foodborne pathogen, is mainly attributed to its ability to form biofilm on food contact surfaces. ε-polylysine, a polymer of positively charged lysine, is reported to inhibit biofilm formation of both gram-positive and gram-negative bacteria. To elucidate the mechanism un...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10575-2

    authors: Shen C,Islam MT,Masuda Y,Honjoh KI,Miyamoto T

    更新日期:2020-06-01 00:00:00

  • Synergy between pretreatment lignocellulose modifications and saccharification efficiency in two brown rot fungal systems.

    abstract::Brown rot wood-degrading fungi distinctly modify lignocellulose and completely hydrolyze polysaccharides (saccharification), typically without secreting an exo-acting glucanase and without removing lignin. Although each step of this two-step approach evolved within the same organism, it is unknown if the early lignoce...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-009-1979-7

    authors: Schilling JS,Tewalt JP,Duncan SM

    更新日期:2009-09-01 00:00:00

  • Identifying and engineering the ideal microbial terpenoid production host.

    abstract::More than 70,000 different terpenoid structures are known so far; many of them offer highly interesting applications as pharmaceuticals, flavors and fragrances, or biofuels. Extraction of these compounds from their natural sources or chemical synthesis is-in many cases-technically challenging with low or moderate yiel...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-019-09892-y

    authors: Moser S,Pichler H

    更新日期:2019-07-01 00:00:00

  • Heterologous expression of pyrroloquinoline quinone (pqq) gene cluster confers mineral phosphate solubilization ability to Herbaspirillum seropedicae Z67.

    abstract::Gluconic acid secretion mediated by the direct oxidation of glucose by pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) is responsible for mineral phosphate solubilization in Gram-negative bacteria. Herbaspirillum seropedicae Z67 (ATCC 35892) genome encodes GDH apoprotein but lacks genes for the bi...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5610-1

    authors: Wagh J,Shah S,Bhandari P,Archana G,Kumar GN

    更新日期:2014-06-01 00:00:00

  • Characterization of catabolic meta-nitrophenol nitroreductase from Cupriavidus necator JMP134.

    abstract::Cupriavidus necator JMP134 utilizes meta-nitrophenol (MNP) as a sole source of carbon, nitrogen, and energy. The metabolic reconstruction of MNP degradation performed in silico suggested that the mnp cluster might have played important roles in MNP degradation. In order to experimentally confirm the prediction, we hav...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-010-2666-4

    authors: Yin Y,Xiao Y,Liu HZ,Hao F,Rayner S,Tang H,Zhou NY

    更新日期:2010-08-01 00:00:00