Identification of LcpRBA3(2), a novel regulator of lcp expression in Streptomyces coelicolor A3(2).

abstract：:The thermostable beta-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding p-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyrano...

journal_title：Applied microbiology and biotechnology

authors： Fourage L,Colas B

更新日期：2001-08-01 00:00:00

abstract：:Wool and silk are major protein fiber materials used by the textile industry. Fiber protein structure-function relationships are briefly described here, and the major enzymatic processing routes for textiles and other novel applications are deeply reviewed. Fiber biomodification is described here with various classes ...

journal_title：Applied microbiology and biotechnology

authors： Fu J,Su J,Wang P,Yu Y,Wang Q,Cavaco-Paulo A

更新日期：2015-12-01 00:00:00

abstract：:Streptomyces tendae Tü 901 produces the nucleoside peptide antibiotic nikkomycin. In shot-gun cloning experiments using pIJ699 as vector we isolated a 9.4-kb DNA fragment from S. tendae which complemented the nikkomycin nonproducing mutant NP9 to the formation of nikkomycin C/Cx and Kx. Nikkomycins were identified by ...

journal_title：Applied microbiology and biotechnology

authors： Bormann C,Aberle K,Fiedler HP,Schrempf H

更新日期：1990-01-01 00:00:00

abstract：:The upstream region of the cellobiohydrolase gene cbhA of Clostridium thermocellum F7 was sequenced. It was found that this region contains the previously sequenced gene celK encoding an enzyme closely related to CbhA (cellulosomal subunit S3). The presence of a putative transcription terminator in the 524-bp intergen...

journal_title：Applied microbiology and biotechnology

authors： Zverlov VV,Velikodvorskaya GA,Schwarz WH,Kellermann J,Staudenbauer WL

更新日期：1999-06-01 00:00:00

abstract：:A reactive iron barrier in a contaminated aquifer with low pH was found to dechlorinate 1,2-dichloroethane (1,2-DCA) in situ. This chlorinated ethane is known to resist abiotic reduction by zero valent iron. Samples taken up-gradient and within the barrier were used to inoculate anaerobic batch cultures amended with v...

journal_title：Applied microbiology and biotechnology

authors： Zemb O,Lee M,Low A,Manefield M

更新日期：2010-09-01 00:00:00

abstract：:L-cysteine, a valuable sulfur-containing amino acid, has been widely used in food, agriculture, and pharmaceutical industries. Due to the toxicity and complex regulation of L-cysteine, no efficient cell factory has yet been achieved for L-cysteine industrial production. In this study, the food-grade microorganism Cory...

journal_title：Applied microbiology and biotechnology

authors： Wei L,Wang H,Xu N,Zhou W,Ju J,Liu J,Ma Y

更新日期：2019-02-01 00:00:00

abstract：:Despite airborne microorganisms representing a relevant fraction of atmospheric suspended particles, only a small amount of information is currently available on their abundance and diversity and very few studies have investigated the environmental factors influencing the structure of airborne bacterial communities. I...

journal_title：Applied microbiology and biotechnology

authors： Bertolini V,Gandolfi I,Ambrosini R,Bestetti G,Innocente E,Rampazzo G,Franzetti A

更新日期：2013-07-01 00:00:00

abstract：:During sporulation, Bacillus thuringiensis produces insecticidal crystal inclusions (Cry proteins) encoded by cry genes. In fed-batch cultures (FBCs), spores and Cry protein yields are usually low, so we therefore studied the pattern of metabolic changes occurring in batch cultures and FBCs of a B. thuringiensis strai...

journal_title：Applied microbiology and biotechnology

authors： López-y-López VE,de la Torre M

更新日期：2005-04-01 00:00:00

abstract：:Streptomyces is an interesting host for the secretory production of recombinant proteins because of its innate capacity to secrete proteins at high level in the culture medium. In this report, we evaluated the importance of the phage-shock protein A (PspA) homologue on the protein secretion yield in Streptomyces livid...

journal_title：Applied microbiology and biotechnology

authors： Vrancken K,De Keersmaeker S,Geukens N,Lammertyn E,Anné J,Van Mellaert L

更新日期：2007-01-01 00:00:00

abstract：:The desire to reduce dependence on the ever diminishing fossil fuel reserves coupled with the impetus towards green energy has seen increased research in biofuels as alternative sources of energy. Lignocellulose materials are one of the most promising feedstocks for advanced biofuels production. However, their utilisa...

journal_title：Applied microbiology and biotechnology

authors： Kudanga T,Le Roes-Hill M

更新日期：2014-08-01 00:00:00

abstract：:This review describes the historical development and current state of metals leaching and sulfide mineral biooxidation by the minerals industries. During the past 20 years commercial processes employing microorganisms for mineral recovery have progressed from rather uncontrolled copper dump leaching to mineral oxidati...

journal_title：Applied microbiology and biotechnology

authors： Olson GJ,Brierley JA,Brierley CL

更新日期：2003-12-01 00:00:00

abstract：:A transcriptional activator for actinorhodin biosynthesis, AtrA, was previously characterized in Streptomyces coelicolor A3(2), and an orthologue of atrA, named aveI, is identified in the Streptomyces avermitilis NRRL8165 genome (Uguru et al., Mol Microbiol, 58:131-150, 2005). In this study, genetic and functional cha...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Lu Y,Chen J,Zhang W,Shu D,Qin Z,Yang S,Jiang W

更新日期：2008-08-01 00:00:00

abstract：:Two bacterial strains, Ralstonia sp. LD35 and Pseudomonas putida DSM 1868, were assayed for their ability to degrade the monocyclic aromatic compounds commonly found in olive mill wastewaters (OMWs). The goal was to study the possibility of employing the two strains in the removal of these recalcitrant and toxic compo...

journal_title：Applied microbiology and biotechnology

authors： Di Gioia D,Fava F,Bertin L,Marchetti L

更新日期：2001-05-01 00:00:00

abstract：:A novel biosuperabsorbent protein hydrogel was prepared from protein-rich alcoholic-alkali soluble parts of zygomycete Rhizomucor pusillus biomass. The fungal protein content was 46.8%, and the lipid content was 13.1%. Extraction of protein from this microorganism through the method applied prevents protein decomposit...

journal_title：Applied microbiology and biotechnology

authors： Majdejabbari S,Barghi H,Taherzadeh MJ

更新日期：2011-12-01 00:00:00

abstract：:Secretion of fusion proteins composed of cytoplasmic protein dihydrofolate reductase (DHFR) and the Escherichia coli alpha-haemolysin (HlyA) C-terminal sequence was examined through the haemolysin secretion machinery of E. coli. DHFR of various lengths was combined with the HlyA C-terminal region, and both secretion a...

journal_title：Applied microbiology and biotechnology

authors： Nakano H,Kawakami Y,Nishimura H

更新日期：1992-09-01 00:00:00

abstract：:The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of bio...

journal_title：Applied microbiology and biotechnology

authors： da Costa JP,Carvalhais V,Ferreira R,Amado F,Vilanova M,Cerca N,Vitorino R

更新日期：2015-09-01 00:00:00

abstract：:A hydraulic retention time (HRT) of 4, 6, and 8 h was employed, respectively, in three reactors to study the effects of HRT on granulation with a newly developed fast granulation strategy, i.e., a strategy by combining strong hydraulic selection pressure with high organic loading rate (OLR). Granules with clear bounda...

journal_title：Applied microbiology and biotechnology

authors： Liu YQ,Zhang X,Zhang R,Liu WT,Tay JH

更新日期：2016-01-01 00:00:00

abstract：:Cordyceps spp. is the herbal medication initially used in China and has been reported as the unique resource of cordycepin. Cordycepin exhibits many health benefits, including anti-photoaging and anti-pigmentation; therefore, it potentially is a bioactive ingredient of cosmetic products. In order to enrich cordycepin ...

journal_title：Applied microbiology and biotechnology

authors： Kunhorm P,Chaicharoenaudomrung N,Noisa P

更新日期：2019-02-01 00:00:00

abstract：:The Escherichia coli RNase G is known as an endoribonuclease responsible for the 5'-end maturation of 16S rRNA and degradation of several specific mRNAs such as adhE and eno mRNAs. In this study, we found that an RNase G mutant derived from the MC1061 strain did not grow on a glucose minimal medium. Genetic analysis r...

journal_title：Applied microbiology and biotechnology

authors： Sakai T,Nakamura N,Umitsuki G,Nagai K,Wachi M

更新日期：2007-08-01 00:00:00

abstract：:Soil microbial autotrophs play a significant role in CO2 fixation in terrestrial ecosystem, particularly in vegetation-constrained ecosystems with environmental stresses, such as the Tibetan Plateau characterized by low temperature and high UV. However, soil microbial autotrophic communities and their driving factors ...

journal_title：Applied microbiology and biotechnology

authors： Guo G,Kong W,Liu J,Zhao J,Du H,Zhang X,Xia P

更新日期：2015-10-01 00:00:00

abstract：:Fungi of the genus Trichoderma are soilborne, green-spored ascomycetes that can be found all over the world. They have been studied with respect to various characteristics and applications and are known as successful colonizers of their habitats, efficiently fighting their competitors. Once established, they launch th...

journal_title：Applied microbiology and biotechnology

authors： Schuster A,Schmoll M

更新日期：2010-07-01 00:00:00

abstract：:Escherichia coli KJ122 was previously engineered to produce high concentration and yield of succinate in mineral salt medium containing glucose and sucrose under anaerobic conditions. However, this strain does not efficiently utilize xylose. To improve the xylose uptake and utilization in the strain KJ122, xylFGH and ...

journal_title：Applied microbiology and biotechnology

authors： Khunnonkwao P,Jantama SS,Kanchanatawee S,Jantama K

更新日期：2018-01-01 00:00:00

abstract：:Biobutanol can be indigenously synthesized by solventogenic Clostridium species; however, these microorganisms possess inferior capability of utilizing abundant and renewable organic wastes, such as starch, lignocellulose, and even syngas. The common strategy to achieve direct butanol production from these organic was...

journal_title：Applied microbiology and biotechnology

authors： Jiang Y,Zhang T,Lu J,Dürre P,Zhang W,Dong W,Zhou J,Jiang M,Xin F

更新日期：2018-07-01 00:00:00

abstract：:The nitrate-dependent denitrifying anaerobic methane oxidation (DAMO) process, which is metabolized together by anaerobic methanotrophic archaea and NC10 phylum bacteria, is expected to be important for the global carbon and nitrogen cycles. However, there are little studies about the existence of this process and the...

journal_title：Applied microbiology and biotechnology

authors： Ding J,Fu L,Ding ZW,Lu YZ,Cheng SH,Zeng RJ

更新日期：2016-01-01 00:00:00

abstract：:The manganese superoxide dismutase gene (sodM) is very highly expressed in Aspergillus oryzae. To elucidate the basis for this high-level expression, deletion analysis of the promoter was undertaken using beta-glucuronidase (GUS) as a reporter. Deletion of a 63-bp sequence from -200 to -138 in the 1,038-bp sodM promot...

journal_title：Applied microbiology and biotechnology

authors： Hisada H,Sano M,Ishida H,Hata Y,Abe Y,Machida M

更新日期：2006-10-01 00:00:00

abstract：:In order to achieve efficient homo L-lactic acid fermentation from xylose, we first carried out addition of xylose assimilation ability to Lactococcus lactis IL 1403 by introducing a plasmid carrying the xylRAB genes from L. lactis IO-1 (pXylRAB). Then modification of xylose assimilation pathway was carried out. L. la...

journal_title：Applied microbiology and biotechnology

authors： Shinkawa S,Okano K,Yoshida S,Tanaka T,Ogino C,Fukuda H,Kondo A

更新日期：2011-09-01 00:00:00

abstract：:Arthrobacter sp. strain KI72 grows on a 6-aminohexanoate oligomer, which is a by-product of nylon-6 manufacturing, as a sole source of carbon and nitrogen. We cloned the two genes, nylD 1 and nylE 1 , responsible for 6-aminohexanoate metabolism on the basis of the draft genomic DNA sequence of strain KI72. We amplifie...

journal_title：Applied microbiology and biotechnology

authors： Takehara I,Fujii T,Tanimoto Y,Kato DI,Takeo M,Negoro S

更新日期：2018-01-01 00:00:00

abstract：:The bacterial phosphoenolpyruvate (PEP)-dependent group translocation system (PTS) requires the presence of both membrane-bound and cytoplasmic components to phosphorylate and translocate sugar. Deinococcus radiodurans has a functional fruA gene coding for the membrane-bound components of the fructose-specific PTS. Ho...

journal_title：Applied microbiology and biotechnology

authors： Lee HY,Magotra M,Wong TY,Chakraborty C,Liu JK

更新日期：2012-02-01 00:00:00

abstract：:Programmable transcriptional regulation is a powerful tool to study gene functions. Current methods to selectively regulate target genes are mainly based on promoter exchange or on overexpressing transcriptional activators. To expand the discovery toolbox, we designed a dCas9-based RNA-guided synthetic transcription a...

journal_title：Applied microbiology and biotechnology

authors： Schüller A,Wolansky L,Berger H,Studt L,Gacek-Matthews A,Sulyok M,Strauss J

更新日期：2020-11-01 00:00:00

abstract：:Many microorganisms, growing on aging flue-cured tobacco leaves, play a part in its fermentation process. These microflora were identified and described by culture-dependent methods earlier. In this study we report the identity of the microflora growing on the tobacco leaf surface by employing culture-independent meth...

journal_title：Applied microbiology and biotechnology

authors： Zhao M,Wang B,Li F,Qiu L,Li F,Wang S,Cui J

更新日期：2007-01-01 00:00:00