当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Redirection of metabolism during nutrient feeding in fed-batch cultures of Bacillus thuringiensis.

Redirection of metabolism during nutrient feeding in fed-batch cultures of Bacillus thuringiensis.

Abstract：

:During sporulation, Bacillus thuringiensis produces insecticidal crystal inclusions (Cry proteins) encoded by cry genes. In fed-batch cultures (FBCs), spores and Cry protein yields are usually low, so we therefore studied the pattern of metabolic changes occurring in batch cultures and FBCs of a B. thuringiensis strain having a cry1Aa promoter-lacZ fusion, and their effect on sporulation and cry1A gene expression. In FBCs, there was a redirection of bacterial metabolism and a reduction in the specific growth rate during feeding, even when the nutrient concentration was higher than at the beginning of batch culture. These physiological changes suggest that the transition state is set up during feeding and this set-up seems to have a negative effect on both sporulation and cry1Aa expression. When the filtrate of a culture in the transition state was added to a batch culture early in the first exponential growth phase, it delayed sporulation and cry1Aa expression, thus suggesting that a soluble cellular factor that blocked sporulation might be excreted during the transition state. Citrate production usually started during the transition state but, when a medium rich in free amino acids was fed, citrate was produced from the first growth phase and sporulation was nearly blocked.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

López-y-López VE,de la Torre M

doi

10.1007/s00253-004-1724-1

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

254-60

issue

2

eissn

0175-7598

issn

1432-0614

journal_volume

67

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • Unravelling glutathione conjugate catabolism in Saccharomyces cerevisiae: the role of glutathione/dipeptide transporters and vacuolar function in the release of volatile sulfur compounds 3-mercaptohexan-1-ol and 4-mercapto-4-methylpentan-2-one.

    abstract：:Sulfur-containing aroma compounds are key contributors to the flavour of a diverse range of foods and beverages, such as wine. The tropical fruit characters of Sauvignon Blanc wines are attributed to the presence of the aromatic thiols 3-mercaptohexan-1-ol (3-MH), its acetate ester 3-mercaptohexyl acetate (3-MHA), and...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6833-5

    authors： Cordente AG,Capone DL,Curtin CD

    更新日期：2015-11-01 00:00:00

  • Towards a biotechnological platform for the production of human pro-angiogenic growth factors in the green alga Chlamydomonas reinhardtii.

    abstract：:The recent use of photosynthetic organisms such as Chlamydomonas reinhardtii in biomedical applications has demonstrated their potential for the treatment of acute and chronic tissue hypoxia. Moreover, transgenic microalgae have been suggested as an alternative in situ drug delivery system. In this study, we set out t...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-019-10267-6

    authors： Jarquín-Cordero M,Chávez MN,Centeno-Cerdas C,Bohne AV,Hopfner U,Machens HG,Egaña JT,Nickelsen J

    更新日期：2020-01-01 00:00:00

  • 2,3-Butanediol production from cellobiose using exogenous beta-glucosidase-expressing Bacillus subtilis.

    abstract：:We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7326-x

    authors： Tanimura K,Takashima S,Matsumoto T,Tanaka T,Kondo A

    更新日期：2016-07-01 00:00:00

  • Composition of activated sludge settling and planktonic bacterial communities treating industrial effluent and their correlation to settling problems.

    abstract：:Problems with deflocculation and solids separation in biological wastewater treatment systems are linked to fluctuations in physicochemical conditions. This study examined the composition of activated sludge bacterial communities in lab-scale sequencing batch reactors treating bleached kraft mill effluent, under trans...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-010-2829-3

    authors： Nadarajah N,Allen DG,Fulthorpe RR

    更新日期：2010-11-01 00:00:00

  • Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence.

    abstract：:This study presents a novel methodology for the development of a chemically defined medium (CDM) using genome-scale metabolic network and flux balance analysis. The genome-based in silico analysis identified two amino acids and four vitamins as non-substitutable essential compounds to be supplemented to a minimal medi...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1425-2

    authors： Song H,Kim TY,Choi BK,Choi SJ,Nielsen LK,Chang HN,Lee SY

    更新日期：2008-05-01 00:00:00

  • A simple method for isolation and construction of markerless cyanobacterial mutants defective in acyl-acyl carrier protein synthetase.

    abstract：:Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) secrete free fatty acids (FFAs) into the external medium and hence have been used for the studies aimed at photosynthetic production of biofuels. While the wild-type strain of Synechocystis sp. PCC 6803 is highly sensitive to exogenously ad...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7850-8

    authors： Kojima K,Keta S,Uesaka K,Kato A,Takatani N,Ihara K,Omata T,Aichi M

    更新日期：2016-12-01 00:00:00

  • Development and production of nanobodies specifically against green fluorescence protein.

    abstract：:Variable domains of heavy chains of camelid heavy-chain antibodies (VHHs) are known as nanobodies. Nanobodies are approximately 15 kDa in size with high affinity to their antigens. They can be easily manipulated and produced in microorganisms. In this study, an alpaca was immunized with purified green fluorescence pro...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-10535-w

    authors： Fang Z,Cao D,Qiu J

    更新日期：2020-06-01 00:00:00

  • Influence of stringent and relaxed response on excretion of recombinant proteins and fatty acid composition in Escherichia coli.

    abstract：:In contrast to stringent (relA+) cells of Escherichia coli, relaxed (relA) cells excreted recombinant proteins (beta-lactamase, interferon alpha 1) into the culture medium during amino acid limitation. Comparative analyses of overall fatty acid composition in relA+ cells and relA cells were performed and revealed that...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00170628

    authors： Gitter B,Diefenbach R,Keweloh H,Riesenberg D

    更新日期：1995-04-01 00:00:00

  • A target-specific chimeric toxin composed of epidermal growth factor and Pseudomonas exotoxin A with a deletion in its toxin-binding domain.

    abstract：:We have fused the epidermal growth factor (EGF) to the amino terminus of Pseudomonas exotoxin A (PE) to create a cytotoxic agent, designated EGF-PE, which preferentially kills EGF-receptor-bearing cells. In this study, we analyzed the effect of the Ia domain, the binding domain of PE on the cytotoxicity of EGF-PE towa...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00218456

    authors： Liao CW,Hseu TH,Hwang J

    更新日期：1995-07-01 00:00:00

  • The pentose phosphate pathway leads to enhanced succinic acid flux in biofilms of wild-type Actinobacillus succinogenes.

    abstract：:Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four d...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7763-6

    authors： Bradfield MF,Nicol W

    更新日期：2016-11-01 00:00:00

  • A general method for identification of polyhydroxyalkanoic acid synthase genes from pseudomonads belonging to the rRNA homology group I.

    abstract：:Using a 30-mer oligonucleotide probe highly specific for polyhydroxyalkanoic acid (PHA) synthase genes, the respective genes of Pseudomonas citronellolis, P. mendocina, Pseudomonas sp. DSM 1650 and Pseudomonas sp. GP4BH1 were cloned from genomic libraries in the cosmid pHC79. A 19.5-kbp and a 22.0-kbp EcoRI restrictio...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00173327

    authors： Timm A,Wiese S,Steinbüchel A

    更新日期：1994-01-01 00:00:00

  • Replacement of pr gene with Japanese encephalitis virus pr using reverse genetics reduces antibody-dependent enhancement of dengue virus 2 infection.

    abstract：:Severe dengue is more likely found during secondary heterologous dengue virus (DENV) infection or primary infection of infants born to dengue-immune mothers and led to the hypothesis of antibody-dependent enhancement (ADE). It has been reported that pre-membrane (prM)-reactive antibodies do not efficiently neutralize ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6819-3

    authors： Wang Y,Si L,Luo Y,Guo X,Zhou J,Fang D,Yan H,Zeng G,Jiang L

    更新日期：2015-11-01 00:00:00

  • Reversion of L-lysine inhibition of penicillin G biosynthesis by 6-oxopiperidine-2-carboxylic acid in Penicillium chrysogenum PQ-96.

    abstract：:6-Oxopiperidine-2-carboxylic acid (OCA; cyclic alpha-aminoadipic acid) reverses the L-lysine inhibition of penicillin G production by Penicillium chrysogenum PQ-96. The reaction probably depends on the recovery of L-alpha-aminoadipic acid for penicillin G production from OCA. ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00170067

    authors： Kurzatkowski W,Kurzatkowski JD,Filipek J,Solecka J,Kuryłowicz W

    更新日期：1990-12-01 00:00:00

  • Anaerobic glycerol production by Saccharomyces cerevisiae strains under hyperosmotic stress.

    abstract：:Glycerol formation is vital for reoxidation of nicotinamide adenine dinucleotide (reduced form; NADH) under anaerobic conditions and for the hyperosmotic stress response in the yeast Saccharomyces cerevisiae. However, relatively few studies have been made on hyperosmotic stress under anaerobic conditions. To study the...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0821-8

    authors： Modig T,Granath K,Adler L,Lidén G

    更新日期：2007-05-01 00:00:00

  • Sequence of the Corynebacterium glutamicum pyruvate carboxylase gene.

    abstract：:Pyruvate carboxylase is an important anaplerotic enzyme replenishing oxaloacetate consumed for biosynthesis during growth, or lysine and glutamic acid production in industrial fermentations. We used regions of homology from pyruvate carboxylase sequences of 12 different species (corresponding to the ATP- and pyruvate-...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530051302

    authors： Koffas MA,Ramamoorthi R,Pine WA,Sinskey AJ,Stephanopoulos G

    更新日期：1998-09-01 00:00:00

  • An on-line technique for monitoring propionic acid fermentation.

    abstract：:An on-line technique, based on measuring the increase in pressure due to CO(2) release in a closed air-tight reactor, was used to evaluate the fermentation of lactate by propionibacteria. The method was applied to batch cultures of Propionibacterium shermanii grown in yeast extract/sodium lactate medium containing lac...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-005-1888-3

    authors： Benjelloun H,Rochex A,Lecouturier D,Dechemi S,Lebeault JM

    更新日期：2005-08-01 00:00:00

  • Production of a novel compound, 10,12-dihydroxystearic acid from ricinoleic acid by an oleate hydratase from Lysinibacillus fusiformis.

    abstract：:A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction condi...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-4728-x

    authors： Seo MH,Kim KR,Oh DK

    更新日期：2013-10-01 00:00:00

  • Synthesis of gamma-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463: functional grape must beverage and dermatological applications.

    abstract：:Agriculture surplus were used as substrates to synthesize gamma-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463 for the manufacture of a functional beverage or as a novel application for dermatological purposes. Dilution of the grape must to 1 or 4% (w/v) of total carbohydrates favored higher cell yield a...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-009-2370-4

    authors： Di Cagno R,Mazzacane F,Rizzello CG,De Angelis M,Giuliani G,Meloni M,De Servi B,Gobbetti M

    更新日期：2010-03-01 00:00:00

  • Production of 10(S)-hydroxy-8(E)-octadecenoic and 7,10(S,S)-hydroxy-8(E)-octadecenoic ethyl esters by Novozym 435 in solvent-free media.

    abstract：:Novozym 435, lipase B from Candida antarctica, was used in this study for the production of ethyl esters. For the first time, trans-hydroxy-fatty acid ethyl esters were synthesized in vitro in solvent-free media. We studied the effects of the substrate-ethanol molar ratio and enzyme synthetic stability of the biocatal...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-5059-7

    authors： Martin-Arjol I,Busquets M,Isbell TA,Manresa A

    更新日期：2013-09-01 00:00:00

  • Recent research progress on microbial L-asparaginases.

    abstract：:L-Asparaginases (EC 3.5.1.1) are enzymes that catalyze the hydrolysis of L-asparagine to L-aspartic acid and found in a variety of organisms from microorganisms to mammals. However, they are mainly expressed and produced by microorganisms. Microbial L-asparaginases have received sustained attention due to their irrepl...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-014-6271-9

    authors： Zuo S,Zhang T,Jiang B,Mu W

    更新日期：2015-02-01 00:00:00

  • Bivariate genome-wide association study of the growth plasticity of Staphylococcus aureus in coculture with Escherichia coli.

    abstract：:Phenotypic plasticity is the capacity to change the phenotype in response to different environments without alteration of the genotype. Despite sufficient evidence that microorganisms have a major role in the fitness and sickness of eukaryotes, there has been little research regarding microbial phenotypic plasticity. ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-10636-6

    authors： Zheng X,Bai J,Ye M,Liu Y,Jin Y,He X

    更新日期：2020-06-01 00:00:00

  • Biodegradation of xenobiotics by anaerobic bacteria.

    abstract：:Xenobiotic biodegradation under anaerobic conditions such as in groundwater, sediment, landfill, sludge digesters and bioreactors has gained increasing attention over the last two decades. This review gives a broad overview of our current understanding of and recent advances in anaerobic biodegradation of five selecte...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-004-1864-3

    authors： Zhang C,Bennett GN

    更新日期：2005-06-01 00:00:00

  • Development of an improved phenylacetaldehyde reductase mutant by an efficient selection procedure.

    abstract：:Chiral alcohols are valuable as diverse chemicals and synthetic intermediate materials. Phenylacetaldehyde reductase (PAR) is an enzyme that converts a wide variety of ketones into chiral alcohols with high optical purity. When an alcohol such as 2-propanol is used as a hydrogen donor, PAR itself will also mediate the...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-5406-8

    authors： Makino Y,Itoh N

    更新日期：2014-05-01 00:00:00

  • Impacts of coexisting antibiotics, antibacterial residues, and heavy metals on the occurrence of erythromycin resistance genes in urban wastewater.

    abstract：:Antibiotic resistance is a global challenge and represents a growing threat on human health worldwide. Wastewater treatment plants (WWTPs) are generally considered as hotspots for control and/or dissemination of antibiotic resistance. The role of antibiotics, antibacterial residues, and heavy metals played on the evol...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6404-9

    authors： Gao P,He S,Huang S,Li K,Liu Z,Xue G,Sun W

    更新日期：2015-05-01 00:00:00

  • The three tricarboxylate synthase activities of Corynebacterium glutamicum and increase of L-lysine synthesis.

    abstract：:Corynebacterium glutamicum owns a citrate synthase and two methylcitrate synthases. Characterization of the isolated enzymes showed that the two methylcitrate synthases have comparable catalytic efficiency, k (cat)/K (m), as the citrate synthase with acetyl-CoA as substrate, although these enzymes are only synthesized...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1105-7

    authors： Radmacher E,Eggeling L

    更新日期：2007-09-01 00:00:00

  • Directed aryl sulfotransferase evolution toward improved sulfation stoichiometry on the example of catechols.

    abstract：:Sulfation is an important way for detoxifying xenobiotics and endobiotics including catechols. Enzymatic sulfation occurs usually with high chemo- and/or regioselectivity under mild reaction conditions. In this study, a two-step p-NPS-4-AAP screening system for laboratory evolution of aryl sulfotransferase B (ASTB) wa...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-019-09688-0

    authors： Ji Y,Islam S,Mertens AM,Sauer DF,Dhoke GV,Jakob F,Schwaneberg U

    更新日期：2019-05-01 00:00:00

  • Bacterial response to acetate challenge: a comparison of tolerance among species.

    abstract：:Although acetate formation and tolerance are important criteria for various aspects of biotechnological process development, available studies on acetate tolerance in different species are disparate. We evaluate the response of eight bacterial strains, including two variants of Escherichia coli, two variants of Staphy...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530000339

    authors： Lasko DR,Zamboni N,Sauer U

    更新日期：2000-08-01 00:00:00

  • An efficient lipase-catalyzed enantioselective hydrolysis of (R,S)-azolides derived from N-protected proline, pipecolic acid, and nipecotic acid.

    abstract：:In the Candida antarctica lipase B-catalyzed hydrolysis of (R,S)-azolides derived from (R,S)-N-protected proline in water-saturated methyl tert-butyl ether (MTBE), high enzyme activity with excellent enantioselectivity (V (S) V (R) ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4010-7

    authors： Wu CH,Pen CW,Wang PY,Tsai SW

    更新日期：2013-02-01 00:00:00

  • Biotechnological methods for chalcone reduction using whole cells of Lactobacillus, Rhodococcus and Rhodotorula strains as a way to produce new derivatives.

    abstract：:Microbial strains of the genera Dietzia, Micrococcus, Pseudomonas, Rhodococcus, Gordonia, Streptomyces, Pseudomonas, Bacillus, Penicillium, Rhodotorula and Lactobacillus were screened for the ability to convert chalcones. Synthesis of chalcones was performed by the Claisen-Schmidt reaction. There were three groups of ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7607-4

    authors： Stompor M,Kałużny M,Żarowska B

    更新日期：2016-10-01 00:00:00

  • Purification and characterization of thermostable H2O2-forming NADH oxidase from 2-phenylethanol-assimilating Brevibacterium sp. KU1309.

    abstract：:A cytoplasmic NADH oxidase (NOX) was purified from a soil bacteria, Brevibacterium sp. KU1309, which is able to grow in the medium containing 2-phenylethanol as the sole source of carbon under an aerobic condition. The enzyme catalyzed the oxidation of NADH to NAD+ involving two-electron reduction of O2 to H2O2. The m...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1535-x

    authors： Hirano J,Miyamoto K,Ohta H

    更新日期：2008-08-01 00:00:00