Directed aryl sulfotransferase evolution toward improved sulfation stoichiometry on the example of catechols.

abstract：:The sludge digestate stabilized by mesophilic anaerobic digestion was further degraded through thermophilic anaerobic digestion using 0-10 % (v/v) of thermophilic, proteolytic Coprothermobacter proteolyticus, and/or methanogenic granular sludge. The results demonstrated that the temperature shift to thermophilic condi...

journal_title：Applied microbiology and biotechnology

authors： Lü F,Li T,Wang T,Shao L,He P

更新日期：2014-01-01 00:00:00

abstract：:Chiral alcohols are valuable as diverse chemicals and synthetic intermediate materials. Phenylacetaldehyde reductase (PAR) is an enzyme that converts a wide variety of ketones into chiral alcohols with high optical purity. When an alcohol such as 2-propanol is used as a hydrogen donor, PAR itself will also mediate the...

journal_title：Applied microbiology and biotechnology

authors： Makino Y,Itoh N

更新日期：2014-05-01 00:00:00

abstract：:Cheese whey (CW), the liquid resulting from the precipitation and removal of milk casein during cheese-making, and the second cheese whey (SCW) derived from the production of cottage and ricotta cheeses are the main byproducts of dairy industry. The major constituent of CW and SCW is lactose, contributing to the high ...

journal_title：Applied microbiology and biotechnology

authors： Zotta T,Solieri L,Iacumin L,Picozzi C,Gullo M

更新日期：2020-04-01 00:00:00

abstract：:A Saccharomyces-cerevisiae-based simultaneous saccharification and fermentation (SSF) of lignocellulosic biomass is limited to an operating temperature of about 37 degrees C, and even a small increase in temperature can have a deleterious effect. This points to a need for a more thermotolerant yeast. To this end, S. c...

journal_title：Applied microbiology and biotechnology

authors： Kadam KL,Schmidt SL

更新日期：1997-12-01 00:00:00

abstract：:Discovery of keratin-degrading enzymes from fungi and bacteria has primarily focused on finding one protease with efficient keratinase activity. Recently, an investigation was conducted of all keratinases secreted from a fungus known to grow on keratinaceous materials, such as feather, horn, and hooves. The study demo...

journal_title：Applied microbiology and biotechnology

authors： Lange L,Huang Y,Busk PK

更新日期：2016-03-01 00:00:00

abstract：:The effect of vitamins on the aerobic degradation and dechlorination of 2-chlorophenol and 4-chlorophenol by Pseudomonas pickettii, strain LD1, and 4-chlorobiphenyl by Pseudomonas sp. strain CPE1 was determined. These microorganisms are capable of using the target compounds as the sole carbon and energy source, but do...

journal_title：Applied microbiology and biotechnology

authors： Kafkewitz D,Fava F,Armenante PM

更新日期：1996-11-01 00:00:00

abstract：:Microbial transglutaminase (MTG) from Streptomyces mobaraensis has been widely used for crosslinking proteins in order to acquire products with improved properties. To improve the yield and enable a facile and efficient purification process, recombinant vectors, harboring various heterologous signal peptide-encoding f...

journal_title：Applied microbiology and biotechnology

authors： Mu D,Lu J,Qiao M,Kuipers OP,Zhu J,Li X,Yang P,Zhao Y,Luo S,Wu X,Jiang S,Zheng Z

更新日期：2018-07-01 00:00:00

abstract：:Diosgenin transformation was studied in Streptomyces virginiae IBL-14, a soil-dwelling bacterium with diosgenin-degrading capacity. All of the derivatives isolated were identified as 4-ene-3-keto steroids. We cloned ChoL, a fragment of a cholesterol oxidase from S. virginiae IBL-14, and used gene-disruption techniques...

journal_title：Applied microbiology and biotechnology

authors： Li B,Wang W,Wang FQ,Wei DZ

更新日期：2010-02-01 00:00:00

abstract：:Indigo is an insoluble blue dye historically used for dyeing textiles. A traditional approach for indigo dyeing involves microbial reduction of polygonum indigo to solubilize it under alkaline conditions; however, the mechanism by which microorganisms reduce indigo remains poorly understood. Here, we aimed to identify...

journal_title：Applied microbiology and biotechnology

authors： Suzuki H,Abe T,Doi K,Ohshima T

更新日期：2018-11-01 00:00:00

abstract：:We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was ...

journal_title：Applied microbiology and biotechnology

authors： Tanimura K,Takashima S,Matsumoto T,Tanaka T,Kondo A

更新日期：2016-07-01 00:00:00

abstract：:The human cell line rF2N78 produces an antibody with a high galactosylation ratio which resembles human IgG. However, it has been observed that the aglycosylated antibody starts to appear when glucose is depleted. To determine whether glucose depletion is a main cause for aglycosylation of the antibody, fed-batch cult...

journal_title：Applied microbiology and biotechnology

authors： Seo JS,Min BS,Kim YJ,Cho JM,Baek E,Cho MS,Lee GM

更新日期：2014-04-01 00:00:00

abstract：:Yeast are important production platforms for the generation of recombinant proteins. Nonetheless, their use has been restricted in the production of therapeutic proteins due to differences in their glycosylation profile with that of higher eukaryotes. The yeast strain Pichia pastoris is an industrially important organ...

journal_title：Applied microbiology and biotechnology

authors： Hopkins D,Gomathinayagam S,Lynaugh H,Stadheim TA,Hamilton SR

更新日期：2014-03-01 00:00:00

abstract：:Lactobacillus species are attractive hosts for the expression of heterologous proteins, antigens, vaccines, and drugs due to their GRAS (generally recognized as safe) status. The bioengineering techniques open new possibilities of improving Lactobacillus strains. In this regard, the control of the gene expression in L...

journal_title：Applied microbiology and biotechnology

authors： Peirotén Á,Landete JM

更新日期：2020-05-01 00:00:00

abstract：:Three bacterial strains utilizing paracetamol as the sole carbon, nitrogen, and energy source were isolated from a paracetamol-degrading aerobic aggregate, and assigned to species of the genera Stenotrophomonas and Pseudomonas. The Stenotrophomonas species have not included any known paracetamol degraders until now. I...

journal_title：Applied microbiology and biotechnology

authors： Zhang L,Hu J,Zhu R,Zhou Q,Chen J

更新日期：2013-04-01 00:00:00

abstract：:Microbial strains of the genera Dietzia, Micrococcus, Pseudomonas, Rhodococcus, Gordonia, Streptomyces, Pseudomonas, Bacillus, Penicillium, Rhodotorula and Lactobacillus were screened for the ability to convert chalcones. Synthesis of chalcones was performed by the Claisen-Schmidt reaction. There were three groups of ...

journal_title：Applied microbiology and biotechnology

authors： Stompor M,Kałużny M,Żarowska B

更新日期：2016-10-01 00:00:00

abstract：:1,3-1,4-beta-D-glucanase is an important endoglycosidase in the brewing and animal feed industries. To achieve high-level expression of recombinant glucanase in Pichia pastoris, we designed sequences encoding the alpha-factor signal peptide from Saccharomyces cerevisiae and the truncated 1,3-1,4-beta-D-glucanase from ...

journal_title：Applied microbiology and biotechnology

authors： Huang H,Yang P,Luo H,Tang H,Shao N,Yuan T,Wang Y,Bai Y,Yao B

更新日期：2008-02-01 00:00:00

abstract：:In natural settings, anaerobic digestion can take place in a wide temperature range, but industrial digesters are usually operated under either mesophilic (~35 °C) or thermophilic (~55 °C) conditions. The ability of anaerobic digestion microbiota to switch from one operating temperature to the other remains poorly doc...

journal_title：Applied microbiology and biotechnology

authors： Chapleur O,Mazeas L,Godon JJ,Bouchez T

更新日期：2016-02-01 00:00:00

abstract：:The secreted proteome of Pichia pastoris X-33 was investigated in methanol-induced cultures with a goal to enhance the secretion and purification of recombinant proteins. In a fed-batch fermentation at 30 °C, more host proteins were found in greater concentrations compared to cultures grown at 25 °C. Protein samples c...

journal_title：Applied microbiology and biotechnology

authors： Huang CJ,Damasceno LM,Anderson KA,Zhang S,Old LJ,Batt CA

更新日期：2011-04-01 00:00:00

abstract：:Alcoholic fermentation of grape must is a complex process, involving several yeast genera and species. The early stages in fermentation are dominated by non-Saccharomyces yeasts that are gradually replaced by the Saccharomyces cerevisiae species, which takes over the fermentation. Quantitative studies have reported th...

journal_title：Applied microbiology and biotechnology

authors： Renault PE,Albertin W,Bely M

更新日期：2013-05-01 00:00:00

abstract：:Certain lactic acid bacteria, especially heterofermentative strains, are capable to produce mannitol under adequate culture conditions. In this study, mannitol production by Lactobacillus reuteri CRL 1101 and Lactobacillus fermentum CRL 573 in modified MRS medium containing a mixture of fructose and glucose in a 6.5:1...

journal_title：Applied microbiology and biotechnology

authors： Rodríguez C,Rimaux T,Fornaguera MJ,Vrancken G,de Valdez GF,De Vuyst L,Mozzi F

更新日期：2012-03-01 00:00:00

abstract：:Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...

journal_title：Applied microbiology and biotechnology

authors： Shen G,Zhang S,Liu X,Jiang Q,Ding W

更新日期：2018-11-01 00:00:00

abstract：:In Escherichia coli, the enzyme called cysteine desulfhydrase (CD), which is responsible for L-cysteine degradation, was investigated by native-PAGE and CD activity staining of crude cell extracts. Analyses with gene-disrupted mutants showed that CD activity resulted from two enzymes: tryptophanase (TNase) encoded by ...

journal_title：Applied microbiology and biotechnology

authors： Awano N,Wada M,Kohdoh A,Oikawa T,Takagi H,Nakamori S

更新日期：2003-08-01 00:00:00

abstract：:Nonribosomal peptides (NRPs), a large family of natural products, possess numerous pharmaceutically significant bioactivities. However, many native microbial producers of NRPs are not cultivable or have low production yields making mass production infeasible. The recombinant production of natural products in a surroga...

journal_title：Applied microbiology and biotechnology

authors： Li J,Jaitzig J,Hillig F,Süssmuth R,Neubauer P

更新日期：2014-01-01 00:00:00

abstract：:Two-dimensional gel electrophoresis was conducted to investigate the effect of H2O2 on whole protein expression in Acinetobacter oleivorans DR1. Functional classification of 13 upregulated proteins using MALDI-TOF mass spectrometry showed relationships with oxidative stress, energy production and conversion, nucleotid...

journal_title：Applied microbiology and biotechnology

authors： Kim J,Cho Y,Jang IA,Park W

更新日期：2015-12-01 00:00:00

abstract：:Biosynthesis has emerged as a frontier technology for fabrication of functionally diverse nanoparticles that possess tremendous therapeutic implications. Various biological resources have already demonstrated their potential to produce nanoparticles with interesting features. Endophytic microbes live in a symbiotic re...

journal_title：Applied microbiology and biotechnology

authors： Rahman S,Rahman L,Khalil AT,Ali N,Zia D,Ali M,Shinwari ZK

更新日期：2019-03-01 00:00:00

abstract：:There are alarming reports of growing microbial resistance to all classes of antimicrobial agents used against different infections. Also the existing classes of anticancer drugs used against different tumours warrant the urgent search for more effective alternative agents for treatment. Broad-spectrum bioactivities o...

journal_title：Applied microbiology and biotechnology

authors： Rai M,Kon K,Ingle A,Duran N,Galdiero S,Galdiero M

更新日期：2014-03-01 00:00:00

abstract：:The genes encoding the mevalonate-based farnesyl pyrophosphate (FPP) biosynthetic pathway were encoded in two operons and expressed in Escherichia coli to increase the production of sesquiterpenes. Inefficient translation of several pathway genes created bottlenecks and led to the accumulation of several pathway inter...

journal_title：Applied microbiology and biotechnology

authors： Nowroozi FF,Baidoo EE,Ermakov S,Redding-Johanson AM,Batth TS,Petzold CJ,Keasling JD

更新日期：2014-02-01 00:00:00

abstract：:In this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>20...

journal_title：Applied microbiology and biotechnology

authors： Mendes FS,González-Pajuelo M,Cordier H,François JM,Vasconcelos I

更新日期：2011-11-01 00:00:00

abstract：:Corynebacterium glutamicum shows a great potential for the production of the polyamide monomer putrescine (1,4-diaminobutane). Previously, we constructed the putrescine-producing strain PUT1 by deletion of argF, the gene for ornithine transcarbamoylase (OTC), and argR, encoding the L-arginine repressor, combined with ...

journal_title：Applied microbiology and biotechnology

authors： Schneider J,Eberhardt D,Wendisch VF

更新日期：2012-07-01 00:00:00

abstract：:Saccharomyces cerevisiae was transformed with the Pichia stipitis CBS 6054 XYL1 and XYL2 genes encoding xylose reductase (XR) and xylitol dehydrogenase (XDH) respectively. The XYL1 and XYL2 genes were placed under the control of the alcohol dehydrogenase 1 (ADH1) and phosphoglycerate kinase (PGK1) promoters in the yea...

journal_title：Applied microbiology and biotechnology

authors： Walfridsson M,Anderlund M,Bao X,Hahn-Hägerdal B

更新日期：1997-08-01 00:00:00