Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly.

abstract：:Actinomycetes are the most important producers of secondary metabolites for medical, agricultural and industrial applications. Efficient engineering of bacterial genomes to improve their biosynthetic capabilities largely depends on the available arsenal of tools and vectors. One of the most widely used vector systems ...

journal_title：Applied microbiology and biotechnology

authors： Muth G

更新日期：2018-11-01 00:00:00

abstract：:This study examines the bioremediation potential and cadmium-induced cellular response on a molecular level in Candida tropicalis 3Aer. Spectroscopic analysis clearly illustrated the involvement of yeast cell wall components in biosorption. Cadmium bioaccumulation was confirmed by TEM, SEM, and EDX examination. TEM im...

journal_title：Applied microbiology and biotechnology

authors： Khan Z,Rehman A,Nisar MA,Zafar S,Hussain SZ,Zerr I,Hussain I,Waseem M,Arif M

更新日期：2017-10-01 00:00:00

abstract：:Fat, oil and, grease (FOG) deposits are one primary cause of sanitary sewer overflows (SSOs). While numerous studies have examined the formation of FOG deposits in sewer pipes, little is known about their biodegradation under sewer environments. In this study, FOG deposit biodegradation potential was determined by stu...

journal_title：Applied microbiology and biotechnology

authors： He X,Zhang Q,Cooney MJ,Yan T

更新日期：2015-07-01 00:00:00

abstract：:Clonal genotypes resistant to fungal diseases are an important component of the cocoa production system in southeastern Bahia state (Brazil), so that technologies for faster production of stronger and healthier plantlets are highly desirable. In this study, the effects of inoculated bacterial endophytes isolated from ...

journal_title：Applied microbiology and biotechnology

authors： Leite HA,Silva AB,Gomes FP,Gramacho KP,Faria JC,de Souza JT,Loguercio LL

更新日期：2013-03-01 00:00:00

abstract：:A recombinant form of the sweet-tasting protein thaumatin has been produced in the filamentous fungus Aspergillus niger var. awamori. Expression cassettes containing a synthetic gene encoding thaumatin II were prepared and used to transform Aspergillus niger var. awamori strain NRRL312. Several fungal strains capable ...

journal_title：Applied microbiology and biotechnology

authors： Faus I,del Moral C,Adroer N,del Río JL,Patiño C,Sisniega H,Casas C,Bladé J,Rubio V

更新日期：1998-04-01 00:00:00

abstract：:Increasing evidence has shown that fecal microbiota transplantation (FMT) could be a promising treatment option for Crohn's disease (CD). However, the frequency of FMT for CD treatment remains unclear. This study aimed to evaluate the optimal timing for administering the second course of FMT to maintain the long-term ...

journal_title：Applied microbiology and biotechnology

authors： Li P,Zhang T,Xiao Y,Tian L,Cui B,Ji G,Liu YY,Zhang F

更新日期：2019-01-01 00:00:00

abstract：:Saccharomyces bayanus, being of interest for wine-making, is not as well known as S. cerevisiae and, due to many changes in the yeast classification, accurate data concerning its metabolic activity are difficult to find. In order to produce this yeast as an active dry yeast to be used as a starter in wine-making, its ...

journal_title：Applied microbiology and biotechnology

authors： Serra A,Strehaiano P,Taillandier P

更新日期：2003-10-01 00:00:00

abstract：:Nitrate, injected into oil fields, can oxidize sulfide formed by sulfate-reducing bacteria (SRB) through the action of nitrate-reducing sulfide-oxidizing bacteria (NR-SOB). When reservoir rock contains siderite (FeCO(3)), the sulfide formed is immobilized as iron sulfide minerals, e.g. mackinawite (FeS). The aim of ou...

journal_title：Applied microbiology and biotechnology

authors： Lin S,Krause F,Voordouw G

更新日期：2009-05-01 00:00:00

abstract：:Bacillus cereus is widely distributed food-borne pathogenic bacterium. Due to the harmness to human hearth and the generation of multidrug-resistant B. cereus, it is urgent to develop novel antimicrobial agents. Phage and phage endolysin were taken as novel antimicrobial substance for their specific lytic activity aga...

journal_title：Applied microbiology and biotechnology

authors： Peng Q,Yuan Y

更新日期：2018-09-01 00:00:00

abstract：:We have successfully created a novel yeast strain able to monitor changes in environmental conditions by displaying either green fluorescent protein (GFP) from Aequorea victoria or blue fluorescent protein (BFP), a variant of GFP, on its cell surface as a visible reporter. For the display of these fluorescent proteins...

journal_title：Applied microbiology and biotechnology

authors： Shibasaki S,Ueda M,Ye K,Shimizu K,Kamasawa N,Osumi M,Tanaka A

更新日期：2001-11-01 00:00:00

abstract：:Many unicellular microorganisms use small signaling molecules to determine their local concentration. The processes involved in the production and recognition of these signals are collectively known as quorum sensing (QS). This form of cell-cell communication is used by unicellular microorganisms to co-ordinate their ...

journal_title：Applied microbiology and biotechnology

authors： Choudhary S,Schmidt-Dannert C

更新日期：2010-05-01 00:00:00

abstract：:Corynebacterium glutamicum, a high GC content gram-positive soil bacterium in Actinobacteria, has been used for the industrial production of amino acids and engineered to produce various compounds, including polymer building blocks and biofuels. Since its genome sequence was first published, its versatile metabolic pa...

journal_title：Applied microbiology and biotechnology

authors： Toyoda K,Inui M

更新日期：2016-01-01 00:00:00

abstract：:The production of infective juvenile stages (IJ) of the entomopathogenic nematode Steinernema carpocapsae in the presence of its symbiotic bacterium Xenorhabdus nematophilus was carried out in orbitally agitated bottles. Four complex culture media (M1-M4) were used, containing from 8% to 28% (by vol.) agave juice (agu...

journal_title：Applied microbiology and biotechnology

authors： Islas-López MA,Sanjuan-Galindo R,Rodríguez-Hernández AI,Chavarría-Hernández N

更新日期：2005-07-01 00:00:00

abstract：:Streptomyces viridochromogenes Tü494 produces the antibiotic phosphinothricyl-alanyl-alanine (Ptt). Ptt-non-producing mutants were isolated following N-methyl-N'-nitro-N-nitrosoguanidine (NTG) or UV light treatment of spore suspensions. In co-synthesis and bioconversion experiments the mutational blocks in the biosynt...

journal_title：Applied microbiology and biotechnology

authors： Alijah R,Dorendorf J,Talay S,Pühler A,Wohlleben W

更新日期：1991-03-01 00:00:00

abstract：:Newcastle disease virus (NDV) is an infectious agent of a large variety of birds, including chicken, which poses a real threat to the agriculture industry. Matrix (M) proteins of NDV and many other viruses perform critical functions during viral assembly and budding from the host cell. M-proteins are well conserved an...

journal_title：Applied microbiology and biotechnology

authors： Iram N,Shah MS,Ismat F,Habib M,Iqbal M,Hasnain SS,Rahman M

更新日期：2014-02-01 00:00:00

abstract：:The environmentally widespread micropollutants bisphenol A (BPA), carbamazepine (CBZ), 17α-ethinylestradiol (EE2), diclofenac (DF), sulfamethoxazole (SMX), technical nonylphenol (t-NP) and triclosan (TCS) were used to assess the potential of the laccase-producing freshwater ascomycete Phoma sp. strain UHH 5-1-03 for m...

journal_title：Applied microbiology and biotechnology

authors： Hofmann U,Schlosser D

更新日期：2016-03-01 00:00:00

abstract：:1,3-Propanediol (1,3-PD) production by fermentation of glycerol was described in 1881 but little attention was paid to this microbial route for over a century. Glycerol conversion to 1,3-PD can be carried out by Clostridia as well as Enterobacteriaceae. The main intermediate of the oxidative pathway is pyruvate, the f...

journal_title：Applied microbiology and biotechnology

authors： Biebl H,Menzel K,Zeng AP,Deckwer WD

更新日期：1999-09-01 00:00:00

abstract：:Levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. To improve the levan production in Bacillus amyloliquefaciens, the regulatory elements of sacB (encoding levansucrase) expression and levansucrase secretion were optimized. Four heterologous pr...

journal_title：Applied microbiology and biotechnology

authors： Gu Y,Zheng J,Feng J,Cao M,Gao W,Quan Y,Dang Y,Wang Y,Wang S,Song C

更新日期：2017-05-01 00:00:00

abstract：:Bacterial contamination and biomass harvesting are still challenges associated with coupling of microalgae and wastewater treatment technology. This study investigated aggregation, bacterial growth, lipid production, and pollutant removal during bacteria contaminated Chlorella regularis cultivation under nutrient star...

journal_title：Applied microbiology and biotechnology

authors： Zhou D,Li Y,Yang Y,Wang Y,Zhang C,Wang D

更新日期：2015-02-01 00:00:00

abstract：:Suspension cultures of the endemic South-African plant Cyclopia subternata were established for the first time and evaluated for the presence of isoflavones. The influence of light, as well as medium supplementation strategies with phenylalanine, casein hydrolysate and coconut water on biomass growth and isoflavone pr...

journal_title：Applied microbiology and biotechnology

authors： Kokotkiewicz A,Luczkiewicz M,Kowalski W,Badura A,Piekus N,Bucinski A

更新日期：2013-10-01 00:00:00

abstract：:This study aimed to investigate the interaction between methane production performance and active microbial community dynamics at different loading rates by increasing influent substrate concentration. The model system was an upflow anaerobic sludge blanket (UASB) reactor using molasses wastewater. The active microbia...

journal_title：Applied microbiology and biotechnology

authors： Yun J,Lee SD,Cho KS

更新日期：2016-05-01 00:00:00

abstract：:Sugar beet pulp is a natural carbon source composed mainly of pectin and cellulose, which is utilized and degraded by the ascomycete Penicillium purpurogenum. The fungus also grows on and degrades acetylated xylan which lacks cellulose and pectin. Both carbon sources have been used in our laboratory to grow the fungus...

journal_title：Applied microbiology and biotechnology

authors： Navarrete M,Callegari E,Eyzaguirre J

更新日期：2012-01-01 00:00:00

abstract：:An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% (3.7 g(DCW) l(-1)-day(-1)) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7--9 mmol l(-1) h(-1). Th...

journal_title：Applied microbiology and biotechnology

authors： Pfruender H,Amidjojo M,Hang F,Weuster-Botz D

更新日期：2005-06-01 00:00:00

abstract：:A gratuitous strain was developed by disrupting the GAL1 gene (galactokinase) of recombinant Saccharomyces cerevisiae harboring the antithrombotic hirudin gene in the chromosome under the control of the GAL10 promoter. A series of glucose-limited fed-batch cultures were carried out to examine the effects of glucose su...

journal_title：Applied microbiology and biotechnology

authors： Kim MD,Lee TH,Lim HK,Seo JH

更新日期：2004-08-01 00:00:00

abstract：:A simple and stable immobilization of a laccase from Pleurotus ostreatus was obtained through genetic fusion with a self-assembling and adhesive class I hydrophobin. The chimera protein was expressed in Pichia pastoris and secreted into the culture medium. The crude culture supernatant was directly used for coatings o...

journal_title：Applied microbiology and biotechnology

authors： Sorrentino I,Giardina P,Piscitelli A

更新日期：2019-04-01 00:00:00

abstract：:The purpose of this paper is to present biomass and nutrient uptake data from Neochloris oleoabundans production in an open trough system. The growth medium used was BG11, temperature ranged from 16.7 °C to 25.3 °C, and pH ranged from 5.52 to 9.94 because the customary pH increase during algal biomass production was m...

journal_title：Applied microbiology and biotechnology

authors： Murray KE,Healy FG,McCord RS,Shields JA

更新日期：2011-04-01 00:00:00

abstract：:In insects, glutathione S-transferases (GSTs) are enzymes involved in detoxification of insecticides. However, few data are available for the codling moth, Cydia pomonella (L.). In this study, we cloned a delta class GST gene CpGSTd1 from C. pomonella. Real-time quantitative PCR shows that CpGSTd1 was up-regulated wit...

journal_title：Applied microbiology and biotechnology

authors： Liu J,Yang X,Zhang Y

更新日期：2014-11-01 00:00:00

abstract：:Heart failure (HF) is a coronary disease that affects people worldwide and has a high mortality rate. N-terminal pro-brain natriuretic peptide (NT-proBNP) has been proven to be a useful and accurate biomarker for diagnosing systolic HF. Here, we report a strategy for the high-level production of recombinant (r)NT-proB...

journal_title：Applied microbiology and biotechnology

authors： Kim YS,Karisa N,Jeon WY,Lee H,Kim YC,Ahn J

更新日期：2019-06-01 00:00:00

abstract：:A beta-lactamase-free penicillin amidase from Alcaligenes sp. active against various beta-lactams was purified to homogeneity. The enzyme can hydrolyze penicillin G to 6-amino penicillanic acid (6-APA) and furnish penicillin G from 6-APA and phenyl acetic acid by condensation. The penicillin amidase is a heterodimer o...

journal_title：Applied microbiology and biotechnology

authors： Das S,Gayen JR,Pal A,Ghosh K,Rosazza JP,Samanta TB

更新日期：2004-08-01 00:00:00

abstract：:Grasslands in semi-arid Northern China are widely desertified, thus inducing the formation of a large area of moving sand lands. Revegetation of the sandy land is commonly adopted to restore degraded grasslands. The structure of the soil microbial community might dramatically change during degradation and recovery bec...

journal_title：Applied microbiology and biotechnology

authors： Cao C,Zhang Y,Cui Z,Feng S,Wang T,Ren Q

更新日期：2017-08-01 00:00:00