The pSG5-based thermosensitive vector family for genome editing and gene expression in actinomycetes.

abstract：:The effect of the presence of supplementary glucose or acetate on the growth and pyridine-degrading activity of freely suspended and calcium-alginate-immobilized Pimelobacter sp. was investigated. Although the supplementary carbon sources could be degraded simultaneously with pyridine, Pimelobacter sp. exhibited a pre...

journal_title：Applied microbiology and biotechnology

authors： Rhee SK,Lee GM,Lee ST

更新日期：1996-02-01 00:00:00

abstract：:Candida albicans is the most common clinical pathogenic fungus, which is highly susceptible to immunodeficiency. Development of novel antifungal agents has become a growing trend in the treatment of Candida infections. C16-Fengycin A, a lipopeptide isolated from Bacillus amyloliquefaciens fmb60 showed significant fung...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Lu J,Sun J,Zhu X,Zhou L,Lu Z,Lu Y

更新日期：2019-11-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHA) are storage polymers accumulated by numerous prokaryotes in form of intracellular granules. Native PHA granules are formed by amorphous polymer which reveals considerably higher elasticity and flexibility as compared to crystalline pure PHA polymers. The fact that bacteria store PHA in amor...

journal_title：Applied microbiology and biotechnology

authors： Sedlacek P,Slaninova E,Enev V,Koller M,Nebesarova J,Marova I,Hrubanova K,Krzyzanek V,Samek O,Obruca S

更新日期：2019-02-01 00:00:00

abstract：:Over 9,000 flavonoid compounds have been found in various plants, comprising one of the largest families of natural products. Flavonoids are an essential factor in plant interactions with the environment, often serving as the first line of defense against UV irradiation and pathogen attacks. Flavonoids are also major ...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Chen S,Yu O

更新日期：2011-08-01 00:00:00

abstract：:Poly(lactate-co-3-hydroxybutyrate) (P(LA-co-3HB)) was previously produced from xylose in engineered Escherichia coli. The aim of this study was to increase the polymer productivity and LA fraction in P(LA-co-3HB) using two metabolic engineering approaches: (1) deletions of competing pathways to lactate production and ...

journal_title：Applied microbiology and biotechnology

authors： Nduko JM,Matsumoto K,Ooi T,Taguchi S

更新日期：2014-03-01 00:00:00

abstract：:The production of acetone, butanol and ethanol by fermentation of renewable biomass has potential to become a valuable industrial process. Mechanisms of solvent production and sporulation involve some common regulators in some ABE-producing clostridia, although details of the links between the pathways are not clear. ...

journal_title：Applied microbiology and biotechnology

authors： Kolek J,Diallo M,Vasylkivska M,Branska B,Sedlar K,López-Contreras AM,Patakova P

更新日期：2017-11-01 00:00:00

abstract：:This study is an application of an experimental design methodology for the optimization of the culture conditions of alpha-L: -arabinofuranosidase production by Talaromyces thermophilus. Wheat bran and yeast extract were first selected as the best carbon and nitrogen sources, respectively, for enzyme production. A Pla...

journal_title：Applied microbiology and biotechnology

authors： Guerfali M,Chaabouni M,Gargouri A,Belghith H

更新日期：2010-02-01 00:00:00

abstract：:The occurrence of killer toxins amongst yeast in Brazilian Riesling Italico grape must was investigated by using the sensitive strain EMBRAPA-26B as a reference strain at 18 degrees C and 28 degrees C. From a total of 85 previously isolated yeasts, 21 strains showed ability to kill the sensitive strain on unbuffered g...

journal_title：Applied microbiology and biotechnology

authors： da Silva GA

更新日期：1996-09-01 00:00:00

abstract：:Comparative metabolic responses of Sinorhizobium sp. C4 were investigated. Comprehensive metabolites profiles, including polar metabolites, fatty acids, and polyhydroxyalkanoates were evaluated through untargeted metabolome analyses. Intracellular metabolomes during the degradation of phenanthrene were compared with t...

journal_title：Applied microbiology and biotechnology

authors： Keum YS,Seo JS,Li QX,Kim JH

更新日期：2008-10-01 00:00:00

abstract：:Seven fractions rich in endoglucanase activity were separated from the extracellular cellulase system of the anaerobic rumen fungus Neocallimastix frontalis. The fractions (ES1, ES3, ES2U1, ES2U2, ES2U4, ES2U3C1 and ES2U3C2) were separated from each other and from a fraction that could solubilize crystalline cellulose...

journal_title：Applied microbiology and biotechnology

authors： Wood TM,Wilson CA,McCrae SI

更新日期：1995-12-01 00:00:00

abstract：:Heart failure (HF) is a coronary disease that affects people worldwide and has a high mortality rate. N-terminal pro-brain natriuretic peptide (NT-proBNP) has been proven to be a useful and accurate biomarker for diagnosing systolic HF. Here, we report a strategy for the high-level production of recombinant (r)NT-proB...

journal_title：Applied microbiology and biotechnology

authors： Kim YS,Karisa N,Jeon WY,Lee H,Kim YC,Ahn J

更新日期：2019-06-01 00:00:00

abstract：:The rapid development of clean fuels for the future is a critically important global challenge for two main reasons. First, new fuels are needed to supplement and ultimately replace depleting oil reserves. Second, fuels capable of zero CO2 emissions are needed to slow the impact of global warming. This review summariz...

journal_title：Applied microbiology and biotechnology

authors： Rupprecht J,Hankamer B,Mussgnug JH,Ananyev G,Dismukes C,Kruse O

更新日期：2006-09-01 00:00:00

abstract：:We collected several biofilm samples from Japanese rivers and established a reproducible multi-species biofilm model that can be analyzed in laboratories. Bacterial abundance at the generic level was highly similar between the planktonic and biofilm communities, whereas comparative metatranscriptomic analysis revealed...

journal_title：Applied microbiology and biotechnology

authors： Nakamura Y,Yamamoto N,Kino Y,Yamamoto N,Kamei S,Mori H,Kurokawa K,Nakashima N

更新日期：2016-08-01 00:00:00

abstract：:In order to understand the intracellular responses in recombinant CHO (rCHO) cells producing antibody in serum-free medium (SFM) supplemented with optimized hydrolysates mixtures, yielding the highest specific growth rate (μ, SFM#S1) or the highest specific antibody productivity (q(Ab,) SFM#S2), differentially express...

journal_title：Applied microbiology and biotechnology

authors： Kim JY,Kim YG,Han YK,Choi HS,Kim YH,Lee GM

更新日期：2011-03-01 00:00:00

abstract：:Exophiala jeanselmei is an opportunistic pathogenic black yeast growing in humid environments such as water reservoirs of air-conditioning systems. Because this fungal contaminant could be vaporized into the air and subsequently cause health problems, its monitoring is recommended. Currently, this monitoring is based ...

journal_title：Applied microbiology and biotechnology

authors： Libert X,Chasseur C,Packeu A,Bureau F,Roosens NH,De Keersmaecker SJC

更新日期：2016-02-01 00:00:00

abstract：:This study was aimed to investigate the effect of a single-chain fragment variable antibody of connective tissue growth factor (anti-CTGF scFv) against the differentiation of fibroblast into myofibroblast. The scFv antibody was firstly expressed in Escherichia coli cells and was then purified by affinity chromatograph...

journal_title：Applied microbiology and biotechnology

authors： Wu G,Wang X,Deng X,Wu P,Xue X,Yan X,Wang X

更新日期：2012-03-01 00:00:00

abstract：:Stirred, pH-controlled anaerobic batch cultures were used to evaluate the in vitro utilisation by canine gut microflora of novel alpha-galactooligosaccharides synthesised with an enzyme extract from a canine Lactobacillus reuteri strain. Fructooligosaccharides (FOS), melibiose and raffinose were used as reference carb...

journal_title：Applied microbiology and biotechnology

authors： Tzortzis G,Goulas AK,Baillon ML,Gibson GR,Rastall RA

更新日期：2004-03-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) employing nucleic acid mimics as probes is becoming an emerging molecular tool in the microbiology area for the detection and visualization of microorganisms. However, the impact that locked nucleic acid (LNA) and 2'-O-methyl (2'-OMe) RNA modifications have on the probe that i...

journal_title：Applied microbiology and biotechnology

authors： Fontenete S,Carvalho D,Guimarães N,Madureira P,Figueiredo C,Wengel J,Azevedo NF

更新日期：2016-07-01 00:00:00

abstract：:Several ansamycins have been reported to inhibit bacterial biofilm formation and accelerate the eradication of developed biofilms, but little is known about the effect of hygrocin C, an ansamycin, on bacterial biofilm formation. Here, hygrocin C was isolated from the marine-derived Streptomyces sp. SCSGAA 0027 and rep...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Nong XH,Amin M,Qi SH

更新日期：2018-02-01 00:00:00

abstract：:Brown rot wood-degrading fungi distinctly modify lignocellulose and completely hydrolyze polysaccharides (saccharification), typically without secreting an exo-acting glucanase and without removing lignin. Although each step of this two-step approach evolved within the same organism, it is unknown if the early lignoce...

journal_title：Applied microbiology and biotechnology

authors： Schilling JS,Tewalt JP,Duncan SM

更新日期：2009-09-01 00:00:00

abstract：:Transcription factors have been studied as an important drug candidate. Ever since the successful generation of induced pluripotent stem cells (iPSCs), there has been tremendous interest in reprogramming transcription factors. Because of the safety risks involved in a virus-based approach, many researchers have been t...

journal_title：Applied microbiology and biotechnology

authors： Ryu J,Park HH,Park JH,Lee HJ,Rhee WJ,Park TH

更新日期：2016-04-01 00:00:00

abstract：:1,3-Propanediol (1,3-PD) production by fermentation of glycerol was described in 1881 but little attention was paid to this microbial route for over a century. Glycerol conversion to 1,3-PD can be carried out by Clostridia as well as Enterobacteriaceae. The main intermediate of the oxidative pathway is pyruvate, the f...

journal_title：Applied microbiology and biotechnology

authors： Biebl H,Menzel K,Zeng AP,Deckwer WD

更新日期：1999-09-01 00:00:00

abstract：:Internal fragments of alpha- and beta-tubulin genes were generated using reverse transcription polymerase chain reaction (RT-PCR), and the termini were isolated using 5'- and 3'-rapid amplification of cDNA ends. Phytophthora capsici alpha- and beta-tubulin specific primers were then used to generate full-length cDNA b...

journal_title：Applied microbiology and biotechnology

authors： Koo BS,Park H,Kalme S,Park HY,Han JW,Yeo YS,Yoon SH,Kim SJ,Lee CM,Yoon MY

更新日期：2009-03-01 00:00:00

abstract：:α-L-Rhamnosyl-β-D-glucosidase (rutinosidase) hydrolyzes the glycosidic linkage between the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose) and the aglycone. We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergi...

journal_title：Applied microbiology and biotechnology

authors： Ishikawa M,Kawasaki M,Shiono Y,Koseki T

更新日期：2018-04-01 00:00:00

abstract：:The genome of the coprophilous fungus Podospora anserina displays an impressive array of genes encoding hemicellulolytic enzymes. In this study, we focused on a putative carbohydrate esterase (CE) from family 16 (CE16) that bears a carbohydrate-binding module from family CBM1. The protein was heterologously expressed ...

journal_title：Applied microbiology and biotechnology

authors： Puchart V,Berrin JG,Haon M,Biely P

更新日期：2015-12-01 00:00:00

abstract：:The dynamics of bacterial communities play an important role in solid-state fermentation (SSF). Poly-γ-glutamic acid (γ-PGA) was produced by Bacillus amyloliquefaciens C1 in SSF using dairy manure compost and monosodium glutamate production residuals as basic substrates. The production of γ-PGA reached a maximum of 0....

journal_title：Applied microbiology and biotechnology

authors： Yong X,Cui Y,Chen L,Ran W,Shen Q,Yang X

更新日期：2011-11-01 00:00:00

abstract：:Ene-reductases originating from extremophiles are gaining importance in the field of biocatalysis due to higher-stability properties. The genome of the acidophilic iron-oxidizing bacterium "Ferrovum" sp. JA12 was found to harbor a thermophilic-like ene-reductase (FOYE-1). The foye-1 gene was ligated into a pET16bp exp...

journal_title：Applied microbiology and biotechnology

authors： Scholtissek A,Ullrich SR,Mühling M,Schlömann M,Paul CE,Tischler D

更新日期：2017-01-01 00:00:00

abstract：:Although the niche differentiation of anammox bacteria has been extensively observed in a lab-scale reactor, the inherent mechanism for this ecological phenomenon is still elusive. Here, we combined the long-term reactor operation, genome-centered metagenome, and metatranscriptome analyses to gain insight into the sub...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Feng Y,Chen L,Niu Z,Liu S

更新日期：2019-10-01 00:00:00

abstract：:The utilization of monochlorobenzoate isomers (2-, 3- and 4-chlorobenzoate) by anaerobic microbial consortia in River Nile sediments was systematically evaluated under denitrifying, Fe-reducing, sulfidogenic and methanogenic conditions. Loss of all three chlorobenzoates was noted in denitrifying cultures; furthermore,...

journal_title：Applied microbiology and biotechnology

authors： Kazumi J,Häggblom MM,Young LY

更新日期：1995-10-01 00:00:00

abstract：:Cultures of Microcoleus consortia polluted with two different types of crude oil, one with high content in aliphatic hydrocarbons (Casablanca) and the other rich in sulphur and aromatic compounds (Maya), were grown for 50 days and studied for changes in oil composition. No toxic effects from these oils were observed o...

journal_title：Applied microbiology and biotechnology

authors： De Oteyza TG,Grimalt JO,Diestra E,Solé A,Esteve I

更新日期：2004-12-01 00:00:00