Metabolic engineering of flavonoids in plants and microorganisms.

abstract：:Aspartic proteases exhibit optimum enzyme activity under acidic conditions and have been extensively used in food, fermentation, and leather industries. In this study, a novel aspartic protease precursor (proTlAPA1) from Talaromyces leycettanus was identified and successfully expressed in Pichia pastoris. Subsequently...

journal_title：Applied microbiology and biotechnology

authors： Guo Y,Tu T,Zheng J,Ren Y,Wang Y,Bai Y,Su X,Wang Y,Yao B,Huang H,Luo H

更新日期：2020-06-01 00:00:00

abstract：:This study reports the development of a novel and simple formulation for probiotic delivery using chitosan-coated agar-gelatin gel particles. This methodology involves the production of agar-gelatin particles by thermally treating a mixture of agar and gelatin solutions at high temperatures (121 °C) and subsequently c...

journal_title：Applied microbiology and biotechnology

authors： Albadran HA,Monteagudo-Mera A,Khutoryanskiy VV,Charalampopoulos D

更新日期：2020-07-01 00:00:00

abstract：:The redox-sensing transcriptional repressor Rex (Rex) displayed diverse functions in different microbial species. Nowadays, only part function of rex has been verified in vitro and alcohol dehydrogenase gene (adhE) as the target of Rex has been widely reported. In this study, rex was knocked out in Thermoanaerobacteri...

journal_title：Applied microbiology and biotechnology

authors： Qu C,Chen L,Li Y,Fu H,Wang J

更新日期：2020-06-01 00:00:00

abstract：:Hepatic lipid metabolic disorders and oxidative stress are involved in the development of non-alcoholic fatty liver disease (NAFLD). This study is to determine the protective effects of Lactobacillus plantarum NA136 on high-fat diet and fructose (HFD/F)-induced NAFLD and to elucidate its underlying molecular mechanism...

journal_title：Applied microbiology and biotechnology

authors： Zhao Z,Wang C,Zhang L,Zhao Y,Duan C,Zhang X,Gao L,Li S

更新日期：2019-07-01 00:00:00

abstract：:Non-covalent interaction of alcohol dehydrogenase with polysaccharides was studied using three neutral and three anionic polysaccharides. The process of interaction of alcohol dehydrogenase with gum Arabic was optimized with respect to the ratio of enzyme to gum Arabic, pH, and molarity of buffer. Alcohol dehydrogenas...

journal_title：Applied microbiology and biotechnology

authors： Jadhav SB,Bankar SB,Granström T,Ojamo H,Singhal RS,Survase SA

更新日期：2014-01-01 00:00:00

abstract：:We used biodegradable plastics as fermentation substrates for the filamentous fungus Aspergillus oryzae. This fungus could grow under culture conditions that contained emulsified poly-(butylene succinate) (PBS) and emulsified poly-(butylene succinate-co-adipate) (PBSA) as the sole carbon source, and could digest PBS a...

journal_title：Applied microbiology and biotechnology

authors： Maeda H,Yamagata Y,Abe K,Hasegawa F,Machida M,Ishioka R,Gomi K,Nakajima T

更新日期：2005-06-01 00:00:00

abstract：:The genomic DNA and cDNA for a gene encoding a novel trehalose synthase (TSase) catalyzing trehalose synthesis from alpha-D-glucose 1-phosphate and D-glucose were cloned from a basidiomycete, Grifola frondosa. Nucleotide sequencing showed that the 732-amino-acid TSase-encoding region was separated by eight introns. Co...

journal_title：Applied microbiology and biotechnology

authors： Saito K,Yamazaki H,Ohnishi Y,Fujimoto S,Takahashi E,Horinouchi S

更新日期：1998-08-01 00:00:00

abstract：:A system for the controlled expression of a foreign gene in cultured tobacco cells (Nicotiana tabacum, BY2) by temperature shift was constructed. A 925-base-pair (bp) DNA fragment containing the 5'-flanking region of a low-molecular-mass heat-shock protein gene (HSP18.2) of Arabidopsis thaliana was inserted upstream o...

journal_title：Applied microbiology and biotechnology

authors： Yoshida K,Kasai T,Garcia MR,Sawada S,Shoji T,Shimizu S,Yamazaki K,Komeda Y,Shinmyo A

更新日期：1995-12-01 00:00:00

abstract：:Ammonia was observed as a potential significant factor to manipulate the abundance and activity of ammonia-oxidizing microorganisms (AOMs) in water environments. For the first time, this study confirmed this phenomenon by laboratory cultivation. In a series of estuarine sediment-coastal water microcosms, we investigat...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Chen L,Dai T,Sun R,Wen D

更新日期：2015-08-01 00:00:00

abstract：:5-Hydroxytryptophan (5-HTP) is the precursor of the neurotransmitter serotonin and has been used for the treatment of various diseases such as depression, insomnia, chronic headaches, and binge eating associated obesity. The production of 5-HTP had been achieved in our previous report, by the development of a recombin...

journal_title：Applied microbiology and biotechnology

authors： Xu D,Fang M,Wang H,Huang L,Xu Q,Xu Z

更新日期：2020-03-01 00:00:00

abstract：:Fatty acids are a promising raw material for substance production because of their highly reduced and anhydrous nature, which can provide higher fermentation yields than sugars. However, they are insoluble in water and are poorly utilized by microbes in industrial fermentation production. We used fatty acids as raw ma...

journal_title：Applied microbiology and biotechnology

authors： Doi H,Hoshino Y,Nakase K,Usuda Y

更新日期：2014-01-01 00:00:00

abstract：:This paper introduces a new type of system to simulate conditions in the large intestine. This system combines removal of metabolites and water with peristaltic mixing to obtain and handle physiological concentrations of microorganisms, dry matter and microbial metabolites. The system has been designed to be complemen...

journal_title：Applied microbiology and biotechnology

authors： Minekus M,Smeets-Peeters M,Bernalier A,Marol-Bonnin S,Havenaar R,Marteau P,Alric M,Fonty G,Huis in't Veld JH

更新日期：1999-12-01 00:00:00

abstract：:The 3D structures of Fim H and PapG proteins complexed with the host carbohydrate receptor demonstrate that both utilize binding-pocket asparagines for contact or stabilization with the carbohydrate. Pretreatment of whole bacteria with asparaginase resulted in decreased fimbriae-mediated attachment to urinary epitheli...

journal_title：Applied microbiology and biotechnology

authors： Wehner MR,Koch SR,Kindinger L,Nelson N,Cowan MM

更新日期：2004-11-01 00:00:00

abstract：:To understand the effect of daily bioaugmentation in full-scale grease interceptors (GIs), we compared the microbial communities occurring in two full-scale GIs during bioaugmented and non-bioaugmented cycles. The changes in microbial communities were determined using terminal restriction fragment length polymorphism ...

journal_title：Applied microbiology and biotechnology

authors： He X,So MJ,de Los Reyes FL 3rd

更新日期：2016-08-01 00:00:00

abstract：:Stirred, pH-controlled anaerobic batch cultures were used to evaluate the in vitro utilisation by canine gut microflora of novel alpha-galactooligosaccharides synthesised with an enzyme extract from a canine Lactobacillus reuteri strain. Fructooligosaccharides (FOS), melibiose and raffinose were used as reference carb...

journal_title：Applied microbiology and biotechnology

authors： Tzortzis G,Goulas AK,Baillon ML,Gibson GR,Rastall RA

更新日期：2004-03-01 00:00:00

abstract：:The essential general features required for lipid membranes of extremophilic archaea to fulfill biological functions are that they are in the liquid crystalline phase and have extremely low permeability of solutes that is much less temperature sensitive due to a lack of lipid-phase transition and highly branched isopr...

journal_title：Applied microbiology and biotechnology

authors： Ulrih NP,Gmajner D,Raspor P

更新日期：2009-08-01 00:00:00

abstract：:Population dynamics was studied in a 52-l biotrickling filter (BTF) operated for 182 days and used to clean air contaminated with styrene vapors. In the BTF, biomass grew either as free-floating (planktonic) or attached (sessile) microorganisms. PCR-amplified 16S rDNA fragments from planktonic and sessile cells within...

journal_title：Applied microbiology and biotechnology

authors： Tresse O,Lorrain MJ,Rho D

更新日期：2002-08-01 00:00:00

abstract：:For the first time, Lactobacillus plantarum strains carrying heterologous genes encoding multifunctional glycoside hydrolases were constructed and used as additives for alfalfa silage. The chemical characteristics, nonstructural carbohydrate composition, and fermentation quality of alfalfa silage were examined. The su...

journal_title：Applied microbiology and biotechnology

authors： Guo J,Xie Y,Yu Z,Meng G,Wu Z

更新日期：2019-10-01 00:00:00

abstract：:In this paper, we critically review the binding protocols currently reported in the literature to measure the affinity of odorants and pheromones to soluble olfactory proteins, such as odorant-binding proteins (OBPs), chemosensory proteins (CSPs) and Niemann-Pick class C2 (NPC2) proteins. The first part contains a bri...

journal_title：Applied microbiology and biotechnology

authors： Pelosi P,Zhu J,Knoll W

更新日期：2018-10-01 00:00:00

abstract：:DNA polymerase I gene was cloned and sequenced from the thermophilic bacterium Geobacillus caldoxylosilyticus TK4. The gene is 2,634 bp long and encodes a protein of 878 amino acids in length. The enzyme has a molecular mass of 99 kDa and shows sequence homology with DNA polymerase I from Bacillus species (89% identit...

journal_title：Applied microbiology and biotechnology

authors： Sandalli C,Singh K,Modak MJ,Ketkar A,Canakci S,Demir I,Belduz AO

更新日期：2009-08-01 00:00:00

abstract：:Cell proliferation is achieved through numerous enzyme reactions. Temperature governs the activity of each enzyme, ultimately determining the optimal growth temperature. The synthesis of useful chemicals and fuels utilizes a fraction of available metabolic pathways, primarily central metabolic pathways including glyco...

journal_title：Applied microbiology and biotechnology

authors： Uchikura H,Toyoda K,Matsuzawa H,Mizuno H,Ninomiya K,Takahashi K,Inui M,Tsuge Y

更新日期：2020-08-01 00:00:00

abstract：:Vitamin B12 is an essential nutrient required for crucial metabolic processes in humans. Vitamin B12-producing lactic acid bacteria (LAB) have been attracting increased attentions currently because of the generally recognized as safe (GRAS) status. Most of recent studies focused on Lactobacillus, and little is known a...

journal_title：Applied microbiology and biotechnology

authors： Li P,Gu Q,Wang Y,Yu Y,Yang L,Chen JV

更新日期：2017-08-01 00:00:00

abstract：:Genetic engineering offers a practical route for enhancing the insect biological control potential of entomopathogenic fungi such as Beauveria bassiana. To date, however, such efforts have relied upon transformation protocols that utilize antibiotic or herbicidal resistance markers as selection agents for the introduc...

journal_title：Applied microbiology and biotechnology

authors： Ying SH,Feng MG,Keyhani NO

更新日期：2013-04-01 00:00:00

abstract：:The white button mushroom Agaricus bisporus is economically the most important commercially produced edible fungus. It is grown on carbon- and nitrogen-rich substrates, such as composted cereal straw and animal manure. The commercial mushroom production process is usually performed in buildings or tunnels under highly...

journal_title：Applied microbiology and biotechnology

authors： Kabel MA,Jurak E,Mäkelä MR,de Vries RP

更新日期：2017-06-01 00:00:00

abstract：:Streptolydigin, a secondary metabolite produced by Streptomyces lydicus, is a potent inhibitor of bacterial RNA polymerases. It has been suggested that streptolydigin biosynthesis is associated with polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS). Thus, there is great interest in understanding the...

journal_title：Applied microbiology and biotechnology

authors： Zhao GR,Luo T,Zhou YJ,Jiang X,Qiao B,Yu FM,Yuan YJ

更新日期：2009-05-01 00:00:00

abstract：:Cheese whey (CW), the liquid resulting from the precipitation and removal of milk casein during cheese-making, and the second cheese whey (SCW) derived from the production of cottage and ricotta cheeses are the main byproducts of dairy industry. The major constituent of CW and SCW is lactose, contributing to the high ...

journal_title：Applied microbiology and biotechnology

authors： Zotta T,Solieri L,Iacumin L,Picozzi C,Gullo M

更新日期：2020-04-01 00:00:00

abstract：:We report on a pheromone-based inter-species communication system, allowing for a controlled cell-cell communication between the two species Saccharomyces cerevisiae and Schizosaccharomyces pombe as a proof of principle. It exploits the mating response pathways of the two yeast species employing the pheromones, α- or ...

journal_title：Applied microbiology and biotechnology

authors： Hennig S,Clemens A,Rödel G,Ostermann K

更新日期：2015-02-01 00:00:00

abstract：:We have recently described the biocatalytic characterization of a self-sufficent biosynthetic alkane hydroxylase based on CYP153A13a from Alcanivorax borkumensis SK2 (thereafter A13-Red). Despite remarkable regio- and chemo-selectivity, A13-Red suffers of a difficult-to-reproduce expression and moderate operational st...

journal_title：Applied microbiology and biotechnology

authors： Bordeaux M,de Girval D,Rullaud R,Subileau M,Dubreucq E,Drone J

更新日期：2014-01-01 00:00:00

abstract：:The production of biological indicators involving bacterial sporulation and multi-step downstream processes has been described. The goal of the present work was to use fermented material as the final product in a biological indicator, thereby reducing processing steps and costs. The performance of three different inex...

journal_title：Applied microbiology and biotechnology

authors： Sella SR,Guizelini BP,Zanello PH,Vandenberghe LP,Ribeiro CA,Minozzo JC,Soccol CR

更新日期：2012-01-01 00:00:00

abstract：:Phomopsis sp. XP-8 is an endophytic fungus that has the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application for the biosynthesis of PDG independent of plants. When cultivated in mung bean medium, PDG production was significantly improved and pinoresinol monoglucoside (PMG) and ...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Shi J,Gao Z,Yangwu R,Jiang H,Che J,Liu Y

更新日期：2015-06-01 00:00:00