Development of chitosan-coated agar-gelatin particles for probiotic delivery and targeted release in the gastrointestinal tract.

abstract：:Pyranose 2-oxidase (P2O) was purified 43-fold to apparent homogeneity from the basidiomycete Phanerochaete chrysosporium using liquid chromatography on phenyl Sepharose, Mono Q (twice) and phenyl Superose. The native enzyme has a molecular mass of about 250 kDa (based on native PAGE) and is composed of four identical ...

journal_title：Applied microbiology and biotechnology

authors： Artolozaga MJ,Kubátová E,Volc J,Kalisz HM

更新日期：1997-05-01 00:00:00

abstract：:Fluoroaromatics are widely and--in recent years--increasingly used as agrochemicals, starting materials for chemical syntheses and especially pharmaceuticals. This originates from the special properties the carbon-fluorine bond is imposing on organic molecules. Hence, fluoro-substituted compounds more and more are con...

journal_title：Applied microbiology and biotechnology

authors： Kiel M,Engesser KH

更新日期：2015-09-01 00:00:00

abstract：:Streptomyces is an interesting host for the secretory production of recombinant proteins because of its innate capacity to secrete proteins at high level in the culture medium. In this report, we evaluated the importance of the phage-shock protein A (PspA) homologue on the protein secretion yield in Streptomyces livid...

journal_title：Applied microbiology and biotechnology

authors： Vrancken K,De Keersmaeker S,Geukens N,Lammertyn E,Anné J,Van Mellaert L

更新日期：2007-01-01 00:00:00

abstract：:Confident protein-DNA interaction (PDI) data could significantly improve our understanding of transcriptional regulation network in both prokaryotes and eukaryotes. New methods need to be established for validating and mining the protein-DNA interaction data produced by bioinformatic tools and large-scale screening as...

journal_title：Applied microbiology and biotechnology

authors： Zeng J,Li Y,Zhang S,He ZG

更新日期：2012-02-01 00:00:00

abstract：:Bacterial cells are protected by an exoskeleton, the stabilizing and shape-maintaining cell wall, consisting of the complex macromolecule peptidoglycan. In view of its function, it could be assumed that the cell wall is a static structure. In truth, however, it is steadily broken down by peptidoglycan-cleaving enzymes...

journal_title：Applied microbiology and biotechnology

authors： Reith J,Mayer C

更新日期：2011-10-01 00:00:00

abstract：:A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using...

journal_title：Applied microbiology and biotechnology

authors： Henschke RB,Henschke EJ,Schmidt FR

更新日期：1991-05-01 00:00:00

abstract：:Under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. The phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. The additi...

journal_title：Applied microbiology and biotechnology

authors： Hollender J,Dreyer U,Kornberger L,Kämpfer P,Dott W

更新日期：2002-01-01 00:00:00

abstract：:The quorum sensing signalling molecules 2-heptyl-3-hydroxy-4(1H)-quinolone, termed the "Pseudomonas quinolone signal" (PQS), and 2-heptyl-4(1H)-quinolone (HHQ) play an important role in the control of virulence gene expression in Pseudomonas aeruginosa. To construct a bioreporter for the specific and sensitive detecti...

journal_title：Applied microbiology and biotechnology

authors： Müller C,Fetzner S

更新日期：2013-01-01 00:00:00

abstract：:Cell-surface functional groups (amino, carboxyl, hydroxyl, as well as phosphate) were chemically modified in various ways to enhance the demulsification capability of the demulsifying bacteria Alcaligenes sp. S-XJ-1. Results demonstrated that the demulsifying activity was significantly inhibited by amino enrichment wi...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Liu J,Huang X,Lu L,Peng K

更新日期：2017-05-01 00:00:00

abstract：:Chemical surfactants may impact microbial cell surface properties, i.e., cell surface hydrophobicity (CSH) and cell surface charge, and may thus affect the uptake of components from non-aqueous phase liquids (NAPLs). This work explored the impact of Triton X-100, Igepal CA 630, and Tween 80 (at twice the critical mice...

journal_title：Applied microbiology and biotechnology

authors： Mohanty S,Mukherji S

更新日期：2012-04-01 00:00:00

abstract：:A cDNA fragment encoding the A catalytic domain of the Neocallimastix frontalis endoxylanase XYN3 was amplified and cloned by the polymerase chain reaction technique. The xyn3A DNA fragment was inserted between the Saccharomyces cerevisiae phosphoglycerate kinase gene promoter and terminator sequences on a multicopy e...

journal_title：Applied microbiology and biotechnology

authors： Durand R,Rascle C,Fèvre M

更新日期：1999-08-01 00:00:00

abstract：:Microbial cell factories offer an economic approach for synthesizing "natural'" aromatic flavor compounds. During their fermentation process, the inefficient synthesis pathway and product cytotoxicity are the major barriers to the high-level production. This study combined metabolic engineering and tolerance engineeri...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Zhang Z,Lu X,Zong H,Zhuge B

更新日期：2020-12-01 00:00:00

abstract：:Small ubiquitin-related modifier (SUMO) technology has been widely used in Escherichia coli expression systems to produce antimicrobial peptides. However, E. coli is a pathogenic bacterium that produces endotoxins and can secrete proteins into the periplasm, forming inclusion bodies. In our work, cathelicidin-BF (CBF)...

journal_title：Applied microbiology and biotechnology

authors： Luan C,Zhang HW,Song DG,Xie YG,Feng J,Wang YZ

更新日期：2014-04-01 00:00:00

abstract：:Based on natural selection and the survival of the fittest by evolutionary adaption, a smart high-throughput system was developed to select active haloalkane dehalogenase variants from a large mutant library. Only active enzyme variants can hydrolyse toxic halogenated alkanes to promote growth, whereas inactive mutant...

journal_title：Applied microbiology and biotechnology

authors： Fibinger MP,Davids T,Böttcher D,Bornscheuer UT

更新日期：2015-11-01 00:00:00

abstract：:Monascus purpureus, a pigment-producing ascomycetous fungus, has been traditionally used for red rice preparation using solid-state fermentation. The objective of this study was to develop an improved pigment-producing strain of M. purpureus MTCC 1090 through genome shuffling followed by detailed analytical estimation...

journal_title：Applied microbiology and biotechnology

authors： Ghosh S,Dam B

更新日期：2020-12-01 00:00:00

abstract：:Agrobacterium tumefaciens has been widely used as a tool for transgenesis in plants. The availability of its genome sequence should facilitate the directed engineering of improved properties; however, the current genome engineering options are laborious. Here, we investigated whether the lambda Red operon can be appli...

journal_title：Applied microbiology and biotechnology

authors： Hu S,Fu J,Huang F,Ding X,Stewart AF,Xia L,Zhang Y

更新日期：2014-03-01 00:00:00

abstract：:The nitrile hydratase (NHase, EC 4.2.1.84) genes (alpha and beta subunit) and the corresponding activator gene from Rhodococcus equi TG328-2 were cloned and sequenced. This Fe-type NHase consists of 209 amino acids (alpha subunit, M(r) 23 kDa) and 218 amino acids (beta subunit, M(r) 24 kDa) and the NHase activator of ...

journal_title：Applied microbiology and biotechnology

authors： Rzeznicka K,Schätzle S,Böttcher D,Klein J,Bornscheuer UT

更新日期：2010-02-01 00:00:00

abstract：:Wastewater produced by the debittering process of green olives (GOW) is rich in polyphenolics and presents high chemical oxygen demand and alkalinity values. Eight white-rot fungi ( Abortiporus biennis, Dichomitus squalens, Inonotus hispidus, Irpex lacteus, Lentinus tigrinus, Panellus stipticus, Pleurotus ostreatus an...

journal_title：Applied microbiology and biotechnology

authors： Aggelis G,Ehaliotis C,Nerud F,Stoychev I,Lyberatos G,Zervakis GI

更新日期：2002-07-01 00:00:00

abstract：:Salicylate is an important intermediate in the bacterial degradation of polycyclic aromatic hydrocarbons and salicylate hydroxylases play essential roles in linking the peripheral and ring-cleavage catabolic pathways. Unlike the well-characterized salicylate 1-hydroxylases, the rarely occurred salicylate 5-hydroxylase...

journal_title：Applied microbiology and biotechnology

authors： Fang T,Zhou NY

更新日期：2014-01-01 00:00:00

abstract：:Using a 30-mer oligonucleotide probe highly specific for polyhydroxyalkanoic acid (PHA) synthase genes, the respective genes of Pseudomonas citronellolis, P. mendocina, Pseudomonas sp. DSM 1650 and Pseudomonas sp. GP4BH1 were cloned from genomic libraries in the cosmid pHC79. A 19.5-kbp and a 22.0-kbp EcoRI restrictio...

journal_title：Applied microbiology and biotechnology

authors： Timm A,Wiese S,Steinbüchel A

更新日期：1994-01-01 00:00:00

abstract：:PCR-based analysis of Bacteroidales 16S rRNA genes has emerged as a promising tool to identify sources of fecal water pollution. In this study, three TaqMan real-time PCR assays (BacGeneral, BacHuman, and BacBovine) were developed and evaluated for their ability to quantitatively detect general (total), human-specific...

journal_title：Applied microbiology and biotechnology

authors： Lee DY,Weir SC,Lee H,Trevors JT

更新日期：2010-12-01 00:00:00

abstract：:We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was ...

journal_title：Applied microbiology and biotechnology

authors： Tanimura K,Takashima S,Matsumoto T,Tanaka T,Kondo A

更新日期：2016-07-01 00:00:00

abstract：:The human antimicrobial peptide LL-37 is a cationic peptide with antimicrobial activity against both Gram-positive and Gram-negative microorganisms. This work describes the development of an expression system based on Escherichia coli capable of high production of the recombinant LL-37. The fusion protein Trx-LL-37 wa...

journal_title：Applied microbiology and biotechnology

authors： Krahulec J,Hyrsová M,Pepeliaev S,Jílková J,Cerný Z,Machálková J

更新日期：2010-09-01 00:00:00

abstract：:(R)-[3,5-bis(trifluoromethyl)phenyl] ethanol ((R)-BTPE) is a valuable chiral intermediate for the synthesis of antiemetic drug Aprepitant and Fosaprepitant. A Leifsonia xyli HS0904-derived carbonyl reductase (LXCAR), an effective biocatalyst for the asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone (BTAP) ...

journal_title：Applied microbiology and biotechnology

authors： Wang NQ,Sun J,Huang J,Wang P

更新日期：2014-10-01 00:00:00

abstract：:Production of a novel antimicrobial tropolone, beta-thujaplicin, in Cupressus lusitanica suspension cultures was studied by using a variety of chemicals and fungal elicitors. Sodium alginate, chitin, and methyl jasmonate resulted in 2-, 2.5-, and 3-fold higher beta-thujaplicin production, respectively, than in the con...

journal_title：Applied microbiology and biotechnology

authors： Zhao J,Fujita K,Yamada J,Sakai K

更新日期：2001-04-01 00:00:00

abstract：:In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-ino...

journal_title：Applied microbiology and biotechnology

authors： Moreno PR,Schlatmann JE,van der Heijden R,van Gulik WM,ten Hoopen HJ,Verpoorte R,Heijnen JJ

更新日期：1993-04-01 00:00:00

abstract：:Two-dimensional gel electrophoresis was conducted to investigate the effect of H2O2 on whole protein expression in Acinetobacter oleivorans DR1. Functional classification of 13 upregulated proteins using MALDI-TOF mass spectrometry showed relationships with oxidative stress, energy production and conversion, nucleotid...

journal_title：Applied microbiology and biotechnology

authors： Kim J,Cho Y,Jang IA,Park W

更新日期：2015-12-01 00:00:00

abstract：:Microbial deterioration accounts for a significant percentage of the degradation processes that occur on archeological/historical objects and artworks, and identifying the causative agents of such a phenomenon should therefore be a priority, in consideration of the need to conserve these important cultural heritage it...

journal_title：Applied microbiology and biotechnology

authors： Mazzoli R,Giuffrida MG,Pessione E

更新日期：2018-08-01 00:00:00

abstract：:Protein titers, a key bioprocessing metric, depend both on the synthesis of protein and the degradation of protein. Secreted recombinant protein production in Saccharomyces cerevisiae is an attractive platform as minimal media can be used for cultivation, thus reducing fermentation costs and simplifying downstream pur...

journal_title：Applied microbiology and biotechnology

authors： Tyo KE,Liu Z,Magnusson Y,Petranovic D,Nielsen J

更新日期：2014-08-01 00:00:00

abstract：:In oyster ponds, the marine diatom Haslea ostrearia synthesises and excretes a hydrosoluble pigment of commercial interest called marennin. During the benthic stage, when algal cells are naturally immobilised in their own polysaccharides, marennin production is higher. To optimise this production, axenic cultures of H...

journal_title：Applied microbiology and biotechnology

authors： Lebeau T,Gaudin P,Moan R,Robert JM

更新日期：2002-07-01 00:00:00