Production of Lactobacillus kefir cells for asymmetric synthesis of a 3,5-dihydroxycarboxylate.

abstract：:The catalytic domain encoded by an adenine-thymine (AT)-rich xylanase gene (xynA) of the anaerobic fungus Orpinomyces was expressed in Hypocrea jecorina under the control of the cel7A promoter and terminator. No XynA protein was detected in H. jecorina culture supernatants when the original sequence was fused to the H...

journal_title：Applied microbiology and biotechnology

authors： Li XL,Skory CD,Ximenes EA,Jordan DB,Dien BS,Hughes SR,Cotta MA

更新日期：2007-04-01 00:00:00

abstract：:CYP102A1 is an efficient medium- to long-chain fatty acid hydroxylase that is able to accept a wide range of non-natural substrates which bear no resemblance to the natural ones. 4-Hexylbenzoic acid (HBA) and 4-nonyloxybenzoic acid (NOBA) were identified as CYP102A1 substrates via screening studies using the BD Oxygen...

journal_title：Applied microbiology and biotechnology

authors： Gudiminchi RK,Smit MS

更新日期：2011-04-01 00:00:00

abstract：:The interest in industrial biotechnology and its importance opens up challenging possibilities of research in this area. Surfactants have long been among the most versatile of process chemicals. Their market is extremely competitive and manufacturers will have to expand their arsenal to develop products for the year 2...

journal_title：Applied microbiology and biotechnology

authors： Cameotra SS,Makkar RS

更新日期：1998-11-01 00:00:00

abstract：:Microbial genome sequencing has left a legacy of annotated yet uncharacterized genes or open reading frames, activities that may have useful applications in health and/or the environment. We are interested in the discovery and characterization of potentially new pectinolytic activities for the enzymatic retting of nat...

journal_title：Applied microbiology and biotechnology

authors： Xiao Z,Boyd J,Grosse S,Beauchemin M,Coupe E,Lau PC

更新日期：2008-04-01 00:00:00

abstract：:β-Glucosidases designated MoCel3A and MoCel3B were successfully overexpressed in Magnaporthe oryzae. MoCel3A and MoCel3B showed optimal activity at 50 °C and pH 5.0-5.5. MoCel3A exhibited higher activity on higher degree of polymerization (DP) oligosaccharides and on β-1,3-linked oligosaccharides than on β-1,4-linked ...

journal_title：Applied microbiology and biotechnology

authors： Takahashi M,Konishi T,Takeda T

更新日期：2011-08-01 00:00:00

abstract：:To reveal the succession procedure of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) community structure in sequencing biofilm batch reactor (SBBR), the molecular biological techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and real-time PCR were applied. DGGE showed that th...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Zhang C,Rong H

更新日期：2014-05-01 00:00:00

abstract：:Phosphate (P)-solubilizing microorganisms as a group form an important part of the microorganisms, which benefit plant growth and development. Growth promotion and increased uptake of phosphate are not the only mechanisms by which these microorganisms exert a positive effect on plants. Microbially mediated solubilizat...

journal_title：Applied microbiology and biotechnology

authors： Vassilev N,Vassileva M,Nikolaeva I

更新日期：2006-06-01 00:00:00

abstract：:Overproduction of recombinant secretory proteins triggers numerous physiological perturbations. Depending on a given heterologous protein characteristics, the producer cell is faced with different challenges which lead to varying responses in terms of its physiology and the target protein production rate. In the prese...

journal_title：Applied microbiology and biotechnology

authors： Korpys-Woźniak P,Kubiak P,Białas W,Celińska E

更新日期：2020-11-01 00:00:00

abstract：:Batch experiments were conducted to assess the biotransformation potential of four hydrocarbon monoterpenes (d-limonene, alpha-pinene, gamma-terpinene, and terpinolene) and four alcohols (arbanol, linalool, plinol, and alpha-terpineol) under aerobic conditions at 23 degrees C. Both forest-soil extract and enriched cul...

journal_title：Applied microbiology and biotechnology

authors： Misra G,Pavlostathis SG,Perdue EM,Araujo R

更新日期：1996-07-01 00:00:00

abstract：:Secretion of fusion proteins composed of cytoplasmic protein dihydrofolate reductase (DHFR) and the Escherichia coli alpha-haemolysin (HlyA) C-terminal sequence was examined through the haemolysin secretion machinery of E. coli. DHFR of various lengths was combined with the HlyA C-terminal region, and both secretion a...

journal_title：Applied microbiology and biotechnology

authors： Nakano H,Kawakami Y,Nishimura H

更新日期：1992-09-01 00:00:00

abstract：:Decolorisation of azo dyes during biological effluent treatment can involve both adsorption to cell biomass and degradation by azo-bond reduction during anaerobic digestion. Degradation is expected to form aromatic amines, which may be toxic and recalcitrant to anaerobic treatment but degradable aerobically. Methods f...

journal_title：Applied microbiology and biotechnology

authors： O'Neill C,Lopez A,Esteves S,Hawkes FR,Hawkes DL,Wilcox S

更新日期：2000-02-01 00:00:00

abstract：:The redox-sensing transcriptional repressor Rex (Rex) displayed diverse functions in different microbial species. Nowadays, only part function of rex has been verified in vitro and alcohol dehydrogenase gene (adhE) as the target of Rex has been widely reported. In this study, rex was knocked out in Thermoanaerobacteri...

journal_title：Applied microbiology and biotechnology

authors： Qu C,Chen L,Li Y,Fu H,Wang J

更新日期：2020-06-01 00:00:00

abstract：:Enterokinase is one of the most frequently used enzymes for the removal of affinity tags from target recombinant proteins. In this study, several fermentation strategies were assayed for the production of human enterokinase in Pichia pastoris under constitutive GAP promoter. Two of them with controlled specific growth...

journal_title：Applied microbiology and biotechnology

authors： Melicherová K,Krahulec J,Šafránek M,Lišková V,Hopková D,Széliová D,Turňa J

更新日期：2017-03-01 00:00:00

abstract：:Fluoroaromatics are widely and--in recent years--increasingly used as agrochemicals, starting materials for chemical syntheses and especially pharmaceuticals. This originates from the special properties the carbon-fluorine bond is imposing on organic molecules. Hence, fluoro-substituted compounds more and more are con...

journal_title：Applied microbiology and biotechnology

authors： Kiel M,Engesser KH

更新日期：2015-09-01 00:00:00

abstract：:The effects of three heavy metals (Co, Ni and Cd) on the growth kinetics of five bacterial strains with different characteristics (Pseudomonas sp., Phaeobacter sp. strain 27-4, Listonella anguillarum, Carnobacterium piscicola and Leuconostoc mesenteroides subsp. lysis) were studied in a batch system. A bivariate model...

journal_title：Applied microbiology and biotechnology

authors： Rial D,Vázquez JA,Murado MA

更新日期：2011-05-01 00:00:00

abstract：:Plant-parasitic nematodes are estimated to cause global annual losses of more than US$ 100 billion. The number of registered nematicides has declined substantially over the last 25 years due to concerns about their non-specific mechanisms of action and hence their potential toxicity and likelihood to cause environment...

journal_title：Applied microbiology and biotechnology

authors： Degenkolb T,Vilcinskas A

更新日期：2016-05-01 00:00:00

abstract：:In this study, we employed TiO₂ enrichment and high accuracy liquid chromatography-mass spectrometry-mass spectrometry to identify the phosphoproteome of Clostridium acetobutyicum ATCC824 in acidogenesis and solventogenesis. As many as 82 phosphopeptides in 61 proteins, with 107 phosphorylated sites on serine, threoni...

journal_title：Applied microbiology and biotechnology

authors： Bai X,Ji Z

更新日期：2012-07-01 00:00:00

abstract：:Yarrowia lipolytica is a promising platform for single cell oil production. It is well-known for its metabolism oriented toward utilization of hydrophobic substrates and accumulation of storage lipids. Multiple copies of DGA2 under constitutive promoter were introduced into the Q4 strain, a quadruple mutant deleted fo...

journal_title：Applied microbiology and biotechnology

authors： Gajdoš P,Nicaud JM,Rossignol T,Čertík M

更新日期：2015-10-01 00:00:00

abstract：:C (2) to C (4) chloroalkanes have been used for a wide range of industrial applications. Consequently, numerous leaks to the environment have occurred. It is generally observed that the lower chlorinated members of the group, containing 1-3 chlorine atoms, accumulate in environments where reductive conditions prevail....

journal_title：Applied microbiology and biotechnology

authors： De Wildeman S,Verstraete W

更新日期：2003-04-01 00:00:00

abstract：:A recent study of the UV-generated glucose-utilizing mutant Ralstonia eutropha G⁺1 comprising transcriptomic and proteomic analyses revealed clear evidence that glucose is transported by the N-acetylglucosamine-specific phosphotransferase system (PTS(Nag)), which is overexpressed in this mutant due to a derepression o...

journal_title：Applied microbiology and biotechnology

authors： Raberg M,Kaddor C,Kusian B,Stahlhut G,Budinova R,Kolev N,Bowien B,Steinbüchel A

更新日期：2012-08-01 00:00:00

abstract：:Sphingobium sp. strain TCM1 can significantly degrade chlorinated organophosphorus flame retardants, such as tris(2-chloroethyl) phosphate. The PhoK of strain TCM1 (Sb-PhoK) is the main alkaline phosphatase (APase) that catalyzes the last step in the degradation pathway. Here, we purified and characterized Sb-PhoK pro...

journal_title：Applied microbiology and biotechnology

authors： Takahashi S,Morooka Y,Kumakura T,Abe K,Kera Y

更新日期：2020-02-01 00:00:00

abstract：:This study aimed to model the inactivation of Lactobacillus brevis DSM 6235 while retaining the viability of yeasts during washing brewer's yeast with phosphoric acid and chlorine dioxide. The independent variables in the acid washing were pH (1-3) and temperature (1-9 °C), whereas in the washing with chlorine dioxide...

journal_title：Applied microbiology and biotechnology

authors： Munford ARG,Chaves RD,Granato D,Sant'Ana AS

更新日期：2020-05-01 00:00:00

abstract：:The gene from Xanthomonas campestris pv. phaseoli that is involved in the C5 pathway of delta-amino-levulinic acid (ALA) of Escherichia coli. Subcloning of deletion fragments from the initial 2.5-kilobase (kb) chromosomal fragment allowed the isolation of a 1.6-kb fragment that could complement the hemM mutation. Nucl...

journal_title：Applied microbiology and biotechnology

authors： Asahara N,Murakami K,Korbrisate S,Hashimoto Y,Murooka Y

更新日期：1994-02-01 00:00:00

abstract：:A histidine oligopeptide (hexa-His) with the ability to chelate divalent heavy metal ions was displayed on the yeast cell surface for the purpose of enhanced adsorption of heavy metal ions. We genetically fused a hexa-His-encoding gene with the gene encoding the C-terminal half of alpha-agglutinin that includes a glyc...

journal_title：Applied microbiology and biotechnology

authors： Kuroda K,Shibasaki S,Ueda M,Tanaka A

更新日期：2001-12-01 00:00:00

abstract：:Production of (R)-3-hydroxybutyric acid [(R)-3-HB] by strain Halomonas sp. KM-1 under successive aeration conditions was investigated. The first aerobic condition allowed both cell growth and intracellular storage of poly-(R)-3-hydroxybutyric acid (PHB). The second microaerobic condition, achieved by reducing the cult...

journal_title：Applied microbiology and biotechnology

authors： Kawata Y,Kawasaki K,Shigeri Y

更新日期：2012-11-01 00:00:00

abstract：:The principal point of this paper is that the discovery of penicillin and the development of the supporting technologies in microbiology and chemical engineering leading to its commercial scale production represent it as the medicine with the greatest impact on therapeutic outcomes. Our nomination of penicillin for th...

journal_title：Applied microbiology and biotechnology

authors： Kardos N,Demain AL

更新日期：2011-11-01 00:00:00

abstract：:Penicillin G acylase from Achromobacter sp. (NPGA) was studied in the enzymatic synthesis of β-lactam antibiotics by kinetically controlled N-acylation. When compared with penicillin acylase of Escherichia coli (PGA), the NPGA was significantly more efficient at syntheses of ampicillin and amoxicillin (higher S/H rati...

journal_title：Applied microbiology and biotechnology

authors： Bečka S,Štěpánek V,Vyasarayani RW,Grulich M,Maršálek J,Plháčková K,Dobišová M,Marešová H,Plačková M,Valešová R,Palyzová A,Datla A,Ashar TK,Kyslík P

更新日期：2014-02-01 00:00:00

abstract：:The causes of Bacillus spore resistance remain unclear. Many structures including a highly compact envelope, low hydration of the protoplast, high concentrations of Ca-chelated dipicolinic acid, and the presence of small acid-soluble spore proteins seem to contribute to resistance. To evaluate the role of internal pro...

journal_title：Applied microbiology and biotechnology

authors： Nguyen Thi Minh H,Guyot S,Perrier-Cornet JM,Gervais P

更新日期：2008-08-01 00:00:00

abstract：:Pyruvate carboxylase is an important anaplerotic enzyme replenishing oxaloacetate consumed for biosynthesis during growth, or lysine and glutamic acid production in industrial fermentations. We used regions of homology from pyruvate carboxylase sequences of 12 different species (corresponding to the ATP- and pyruvate-...

journal_title：Applied microbiology and biotechnology

authors： Koffas MA,Ramamoorthi R,Pine WA,Sinskey AJ,Stephanopoulos G

更新日期：1998-09-01 00:00:00

abstract：:Repetitious gene cassettes that encode the consensus decapeptide repeat of Mytilus edulis bioadhesive protein were designed, constructed, and expressed in Escherichia coli. The bioadhesive precursor (BP) with a relative molecular mass of 25,000 was expressed from one 600-bp gene at levels approaching 60% of total cell...

journal_title：Applied microbiology and biotechnology

authors： Salerno AJ,Goldberg I

更新日期：1993-05-01 00:00:00