Effects of three heavy metals on the bacteria growth kinetics: a bivariate model for toxicological assessment.


:The effects of three heavy metals (Co, Ni and Cd) on the growth kinetics of five bacterial strains with different characteristics (Pseudomonas sp., Phaeobacter sp. strain 27-4, Listonella anguillarum, Carnobacterium piscicola and Leuconostoc mesenteroides subsp. lysis) were studied in a batch system. A bivariate model, function of time and dose, is proposed to describe simultaneously all the kinetic profiles obtained by incubating a microorganism at increasing concentrations of individual metals. This model combines the logistic equation for describing growth, with a modification of the cumulative Weibull's function for describing the dose-dependent variations of growth parameters. The comprehensive model thus obtained--which minimizes the effects of the experimental error--was statistically significant in all the studied cases, and it raises doubts about toxicological evaluations that are based on a single growth parameter, especially if it is not obtained from a kinetic equation. In lactic acid bacteria cultures (C. piscicola and L. mesenteroides), Cd induced remarkable differences in yield and time course of characteristic metabolites. A global parameter is defined (ED(50,τ): dose of toxic chemical that reduces the biomass of a culture by 50% compared to that produced by the control at the time corresponding to its semi maximum biomass) that allows comparing toxic effects on growth kinetics using a single value.


Rial D,Vázquez JA,Murado MA




Has Abstract


2011-05-01 00:00:00












  • Characterisation of a glucose phosphotransferase system in Clostridium acetobutylicum ATCC 824.

    abstract::The transport of glucose by the solventogenic anaerobe Clostridium acetobutylicum was investigated. Glucose phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity was detected in extracts prepared from cultures grown on glucose and extract fractionation revealed that both soluble and membrane com...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Tangney M,Mitchell WJ

    更新日期:2007-02-01 00:00:00

  • Metabolic engineering of Saccharomyces cerevisiae for production of novel lipid compounds.

    abstract::The yeast Saccharomyces cerevisiae has been modified successfully for production of numerous metabolites and therapeutic proteins through metabolic engineering, but has not been utilized to date for the production of lipid-derived compounds. We developed a lipid metabolic engineering strategy in S. cerevisiae based up...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Dyer JM,Chapital DC,Kuan JW,Mullen RT,Pepperman AB

    更新日期:2002-07-01 00:00:00

  • Stress responses linked to antimicrobial resistance in Acinetobacter species.

    abstract::Since the last 20 years, bacteria of the genus Acinetobacter have been the leading cause of hospital-acquired infections. In addition to the ability of Acinetobacter species to acquire rapid antibiotic resistance, limited knowledge on the mechanisms of multidrug resistance to antibiotics limits the treatment options f...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Shin B,Park C,Park W

    更新日期:2020-02-01 00:00:00

  • Response of microbial community structure to microbial plugging in a mesothermic petroleum reservoir in China.

    abstract::Microbial plugging, a microbial enhancement of oil recovery (MEOR) technique, has been applied in a candidate oil reservoir of Daqing Oil Field (China). The goal of this study is to monitor the survival of injected bacteria and reveal the response of microbial communities in field trial of microbial plugging through i...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhang F,She YH,Ma SS,Hu JM,Banat IM,Hou du J

    更新日期:2010-12-01 00:00:00

  • Metaproteomic strategies and applications for gut microbial research.

    abstract::The human intestine hosts various complex microbial communities that are closely associated with multiple health and disease processes. Determining the composition and function of these microbial communities is critical to unveil disease mechanisms and promote human health. Recently, meta-omic strategies have been dev...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Xiao M,Yang J,Feng Y,Zhu Y,Chai X,Wang Y

    更新日期:2017-04-01 00:00:00

  • Regulation of mosquitocidal toxin synthesis in Bacillus sphaericus.

    abstract::Translational lacZ fusions to the promoters of the parasporal, crystal protein (binary toxin) and 100-kDa ADP-ribosylating mosquitocidal toxin genes of Bacillus sphaericus were prepared and expression of the toxin genes monitored as beta-galactosidase activity. Transcription of the crystal protein gene fusion began im...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Ahmed HK,Mitchell WJ,Priest FG

    更新日期:1995-05-01 00:00:00

  • Effects of vanillin, quillaja saponin, and essential oils on in vitro fermentation and protein-degrading microorganisms of the rumen.

    abstract::This study investigated the effects of vanillin on methanogenesis and rumen fermentation, and the responses of ruminal protein-degrading bacteria to vanillin (at concentrations of 0, 0.76 and 1.52 g/L), essential oils (clove oil, 1 g/L; origanum oil, 0.50 g/L, and peppermint oil, 1 g/L), and quillaja saponin (at conce...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Patra AK,Yu Z

    更新日期:2014-01-01 00:00:00

  • Biotechnological methods for chalcone reduction using whole cells of Lactobacillus, Rhodococcus and Rhodotorula strains as a way to produce new derivatives.

    abstract::Microbial strains of the genera Dietzia, Micrococcus, Pseudomonas, Rhodococcus, Gordonia, Streptomyces, Pseudomonas, Bacillus, Penicillium, Rhodotorula and Lactobacillus were screened for the ability to convert chalcones. Synthesis of chalcones was performed by the Claisen-Schmidt reaction. There were three groups of ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Stompor M,Kałużny M,Żarowska B

    更新日期:2016-10-01 00:00:00

  • Characterization of beta-glucosidase from a strain of Penicillium purpurogenum KJS506.

    abstract::A novel beta-glucosidase (BGL)-producing strain was isolated and identified as Penicillium purpurogenum KJS506 based on its morphology and internal transcribed spacer (ITS) rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, the maximal BGL activity of 12.3...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Jeya M,Joo AR,Lee KM,Tiwari MK,Lee KM,Kim SH,Lee JK

    更新日期:2010-05-01 00:00:00

  • Correlation of nitric oxide produced by an inducible nitric oxide synthase-like protein with enhanced expression of the phenylpropanoid pathway in Inonotus obliquus cocultured with Phellinus morii.

    abstract::Fungal interspecific interactions enhance biosynthesis of phenylpropanoid metabolites (PM), and production of nitric oxide (NO) is known to be involved in this process. However, it remains unknown which signaling pathway(s) or regulator(s) mediate fungal PM biosynthesis. In this study, we cocultured two white-rot fung...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhao Y,Xi Q,Xu Q,He M,Ding J,Dai Y,Keller NP,Zheng W

    更新日期:2015-05-01 00:00:00

  • Heterologous signal peptides-directing secretion of Streptomyces mobaraensis transglutaminase by Bacillus subtilis.

    abstract::Microbial transglutaminase (MTG) from Streptomyces mobaraensis has been widely used for crosslinking proteins in order to acquire products with improved properties. To improve the yield and enable a facile and efficient purification process, recombinant vectors, harboring various heterologous signal peptide-encoding f...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Mu D,Lu J,Qiao M,Kuipers OP,Zhu J,Li X,Yang P,Zhao Y,Luo S,Wu X,Jiang S,Zheng Z

    更新日期:2018-07-01 00:00:00

  • Production and characterization of medium-chain-length polyhydroxyalkanoate with high 3-hydroxytetradecanoate monomer content by fadB and fadA knockout mutant of Pseudomonas putida KT2442.

    abstract::Medium-chain-length polyhydroxyalkanoates (mcl-PHA) consisting of 3-hydroxyhexanoate (HHx), 3-hydroxyoctanoate (HO), 3-hydroxydecanoate, 3-hydroxydodecanoate, and high-content 3-hydroxytetradecanoate (HTD) was produced by knockout mutant Pseudomonas putida KT2442 termed P. putida KTOY06. When grown on 6 to14 g/L singl...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Liu W,Chen GQ

    更新日期:2007-10-01 00:00:00

  • Biosynthesis of bismuth selenide nanoparticles using chalcogen-metabolizing bacteria.

    abstract::Cost and energy reductions in the production process of bismuth chalcogenide (BC) semiconductor materials are essential to make thermoelectric generators comprised of BCs profitable and CO2 neutral over their life cycle. In this study, as an eco-friendly production method, bismuth selenide (Bi2Se3) nanoparticles were ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kuroda M,Suda S,Sato M,Ayano H,Ohishi Y,Nishikawa H,Soda S,Ike M

    更新日期:2019-11-01 00:00:00

  • Genetic reidentification of the pectinolytic yeast strain SCPP as Saccharomyces bayanus var. uvarum.

    abstract::Using genetic hybridization analysis, pulsed-field gel electrophoresis of chromosomal DNA and PCR/RFLP analysis of the MET2 gene, we reidentified 11 Champagne yeast strains. Two of them, SCPP and SC4, were found to belong to Saccharomyces bayanus var. uvarum and the remaining strains to S. cerevisiae. Strain ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Naumov GI,Naumova ES,Aigle M,Masneuf I,Belarbi A

    更新日期:2001-01-01 00:00:00

  • Identification of the flavin monooxygenase responsible for ipso substitution of alkyl and alkoxyphenols in Sphingomonas sp. TTNP3 and Sphingobium xenophagum Bayram.

    abstract::We previously showed that opdA from Sphingomonas sp. PWE1 encodes a putative flavin monooxygenase capable of transforming octylphenol (OP) via type II ipso substitution. Here, we demonstrate that an opdA homolog is responsible for OP and related alkyl/alkoxyphenol degradation in the nonylphenol degrader Sphingomonas s...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Porter AW,Campbell BR,Kolvenbach BA,Corvini PF,Benndorf D,Rivera-Cancel G,Hay AG

    更新日期:2012-04-01 00:00:00

  • Duplicated Clostridium thermocellum cellobiohydrolase gene encoding cellulosomal subunits S3 and S5.

    abstract::The upstream region of the cellobiohydrolase gene cbhA of Clostridium thermocellum F7 was sequenced. It was found that this region contains the previously sequenced gene celK encoding an enzyme closely related to CbhA (cellulosomal subunit S3). The presence of a putative transcription terminator in the 524-bp intergen...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zverlov VV,Velikodvorskaya GA,Schwarz WH,Kellermann J,Staudenbauer WL

    更新日期:1999-06-01 00:00:00

  • Alkaline iron(III) reduction by a novel alkaliphilic, halotolerant, Bacillus sp. isolated from salt flat sediments of Soap Lake.

    abstract::A halotolerant, alkaliphilic dissimilatory Fe(III)-reducing bacterium, strain SFB, was isolated from salt flat sediments collected from Soap Lake, WA. 16S ribosomal ribonucleic acid gene sequence analysis identified strain SFB as a novel Bacillus sp. most similar to Bacillus agaradhaerens (96.7% similarity). Strain SF...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Pollock J,Weber KA,Lack J,Achenbach LA,Mormile MR,Coates JD

    更新日期:2007-12-01 00:00:00

  • Aerobic biodegradation of selected monoterpenes.

    abstract::Batch experiments were conducted to assess the biotransformation potential of four hydrocarbon monoterpenes (d-limonene, alpha-pinene, gamma-terpinene, and terpinolene) and four alcohols (arbanol, linalool, plinol, and alpha-terpineol) under aerobic conditions at 23 degrees C. Both forest-soil extract and enriched cul...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Misra G,Pavlostathis SG,Perdue EM,Araujo R

    更新日期:1996-07-01 00:00:00

  • Light-regulated promoters for tunable, temporal, and affordable control of fungal gene expression.

    abstract::Regulatable promoters are important genetic tools, particularly for assigning function to essential and redundant genes. They can also be used to control the expression of enzymes that influence metabolic flux or protein secretion, thereby optimizing product yield in bioindustry. This review will focus on regulatable ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Fuller KK,Dunlap JC,Loros JJ

    更新日期:2018-05-01 00:00:00

  • Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor. II. Plasmid transfer in mixed cultures.

    abstract::The transfer of plasmids was studied in a stirred fermentor in the course of mixed batch cultures combining recombinant strains of Lactococcus lactis subsp. lactis (donor strains) with L. lactis subsp. lactis CNRZ 268M3 (recipient strain). Donor strains contained one or two of the following plasmids (coding for erythr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: el Alami N,Boquien CY,Corrieu G

    更新日期:1992-06-01 00:00:00

  • Impacts of coexisting antibiotics, antibacterial residues, and heavy metals on the occurrence of erythromycin resistance genes in urban wastewater.

    abstract::Antibiotic resistance is a global challenge and represents a growing threat on human health worldwide. Wastewater treatment plants (WWTPs) are generally considered as hotspots for control and/or dissemination of antibiotic resistance. The role of antibiotics, antibacterial residues, and heavy metals played on the evol...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Gao P,He S,Huang S,Li K,Liu Z,Xue G,Sun W

    更新日期:2015-05-01 00:00:00

  • Reduction and partial degradation mechanisms of naphthylaminesulfonic azo dye amaranth by Shewanella decolorationis S12.

    abstract::Reduction and biodegradation mechanisms of naphthylaminesulfonic azo dye amaranth using a newly isolated Shewanella decolorationis strain S12 were investigated. Under anaerobic conditions, amaranth was reduced by strain S12, and a stoichiometric amount of two reduction products RP-1 and RP-2 were generated. UV/visible...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Hong Y,Guo J,Xu Z,Mo C,Xu M,Sun G

    更新日期:2007-06-01 00:00:00

  • Fermentation of protopanaxadiol type ginsenosides (PD) with probiotic Bifidobacterium lactis and Lactobacillus rhamnosus.

    abstract::Ginsenosides are believed to be the principal components behind the pharmacological actions of ginseng, and their bioactive properties are closely related to the type, position, and number of sugar moieties attached to the aglycone; thus, modification of the sugar chains may markedly change their biological activities...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Tan JS,Yeo CR,Popovich DG

    更新日期:2017-07-01 00:00:00

  • Novel extracellular medium-chain-length polyhydroxyalkanoate depolymerase from Streptomyces exfoliatus K10 DSMZ 41693: a promising biocatalyst for the efficient degradation of natural and functionalized mcl-PHAs.

    abstract::Cloning and biochemical characterization of a novel extracellular medium-chain-length polyhydroxyalkanoate (mcl-PHA) depolymerase from Streptomyces exfoliatus K10 DSMZ 41693 are described. The primary structure of the depolymerase (PhaZSex2) includes the lipase consensus sequence (serine-histidine-aspartic acid) which...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Martínez V,de Santos PG,García-Hidalgo J,Hormigo D,Prieto MA,Arroyo M,de la Mata I

    更新日期:2015-11-01 00:00:00

  • Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller".

    abstract::Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Bräu B,Hilgenfeld R,Schlingmann M,Marquardt R,Birr E,Wohlleben W,Aufderheide K,Pühler A

    更新日期:1991-01-01 00:00:00

  • An on-line technique for monitoring propionic acid fermentation.

    abstract::An on-line technique, based on measuring the increase in pressure due to CO(2) release in a closed air-tight reactor, was used to evaluate the fermentation of lactate by propionibacteria. The method was applied to batch cultures of Propionibacterium shermanii grown in yeast extract/sodium lactate medium containing lac...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Benjelloun H,Rochex A,Lecouturier D,Dechemi S,Lebeault JM

    更新日期:2005-08-01 00:00:00

  • The quest for microbial reductive dechlorination of C (2) to C (4) chloroalkanes is warranted.

    abstract::C (2) to C (4) chloroalkanes have been used for a wide range of industrial applications. Consequently, numerous leaks to the environment have occurred. It is generally observed that the lower chlorinated members of the group, containing 1-3 chlorine atoms, accumulate in environments where reductive conditions prevail....

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: De Wildeman S,Verstraete W

    更新日期:2003-04-01 00:00:00

  • Expression studies with the bidirectional pcbAB-pcbC promoter region from Acremonium chrysogenum using reporter gene fusions.

    abstract::Two cephalosporin genes from Acremonium chrysogenum, pcbAB and pcbC encode the ACV (alpha-aminoadipyl-cysteinyl-valine) synthetase and isopenicillin N-synthetase, respectively. The two adjacent genes are orientated in opposite directions on the chromosomal DNA, separated by a 1.2-kb non-translated sequence, carrying t...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Menne S,Walz M,Kück U

    更新日期:1994-10-01 00:00:00

  • Improving mycelial morphology and adherent growth as well as metabolism of Monascus yellow pigments using nitrate resources.

    abstract::Mycelial adhesion affects cell growth and the production of water-soluble extracellular yellow pigment (EYP) in submerged fermentation with Monascus ruber CGMCC 10910. Two nitrates, NaNO3 and KNO3, were used as nitrogen sources for mitigating mycelial adhesion and improving the production of EYP in this study. The res...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Yang SZ,Huang ZF,Liu HQ,Hu X,Wu ZQ

    更新日期:2020-11-01 00:00:00

  • Lactate and glucose concomitant consumption as a self-regulated pH detoxification mechanism in HEK293 cell cultures.

    abstract::One of the most important limitations of mammalian cell-based processes is the secretion and accumulation of lactate as a by-product of their metabolism. Among the cell lines commonly used in industrial bioprocesses, HEK293 has been gaining importance over the last years. Up recently, HEK293 cells were known to consum...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Liste-Calleja L,Lecina M,Lopez-Repullo J,Albiol J,Solà C,Cairó JJ

    更新日期:2015-12-01 00:00:00