Penicillin G acylase from Achromobacter sp. CCM 4824 : an efficient biocatalyst for syntheses of beta-lactam antibiotics under conditions employed in large-scale processes.

abstract：:Human epidermal growth factor receptors (EGFR) are an important target in drug discovery in terms of both protein-small-molecule interactions and protein-protein interactions. In this work, the isolation of a stable soluble protein of the tyrosine kinase domain of EGFR in Escherichia coli expression has been accomplis...

journal_title：Applied microbiology and biotechnology

authors： Seetaha S,Ratanabanyong S,Choowongkomon K

更新日期：2019-10-01 00:00:00

abstract：:Ethanol fermentation ability of the thermotolerant yeast Kluyveromyces marxianus, which is able to utilize various sugars including glucose, mannose, galactose, xylose, and arabinose, was examined under shaking and static conditions at high temperatures. The yeast was found to produce ethanol from all of these sugars ...

journal_title：Applied microbiology and biotechnology

authors： Rodrussamee N,Lertwattanasakul N,Hirata K,Suprayogi,Limtong S,Kosaka T,Yamada M

更新日期：2011-05-01 00:00:00

abstract：:The filamentous fungus Aspergillus flavus is an agriculturally important opportunistic plant pathogen that produces potent carcinogenic compounds called aflatoxins. We identified the A. flavus rtfA gene, the ortholog of rtf1 in Saccharomyces cerevisiae and rtfA in Aspergillus nidulans. Interestingly, rtfA has multiple...

journal_title：Applied microbiology and biotechnology

authors： Lohmar JM,Harris-Coward PY,Cary JW,Dhingra S,Calvo AM

更新日期：2016-06-01 00:00:00

abstract：:The production of infective juvenile stages (IJ) of the entomopathogenic nematode Steinernema carpocapsae in the presence of its symbiotic bacterium Xenorhabdus nematophilus was carried out in orbitally agitated bottles. Four complex culture media (M1-M4) were used, containing from 8% to 28% (by vol.) agave juice (agu...

journal_title：Applied microbiology and biotechnology

authors： Islas-López MA,Sanjuan-Galindo R,Rodríguez-Hernández AI,Chavarría-Hernández N

更新日期：2005-07-01 00:00:00

abstract：:Chitin was purified from Penaeus monodon and Crangon crangon shells using a two-stage fermentation process with anaerobic deproteination followed by decalcification through homofermentative lactic acid fermentation. Deproteinating enrichment cultures from sewage sludge and ground meat (GM) were used with a proteolytic...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Gallert C,Winter J

更新日期：2008-06-01 00:00:00

abstract：:Butenyl-spinosyn produced by Saccharopolyspora pogona exhibits strong insecticidal activity and broad pesticidal spectrum. However, its synthetic level was low in the wild-type strain. At present, important functional genes involved in butenyl-spinosyn biosynthesis remain unknown, which leads to difficulty in efficien...

journal_title：Applied microbiology and biotechnology

authors： Rang J,Zhu Z,Li Y,Cao L,He H,Tang J,Hu J,Chen J,Hu S,Huang W,Yu Z,Ding X,Sun Y,Xie Q,Xia L

更新日期：2021-01-23 00:00:00

abstract：:2-Phenylethanol (2-PE) is an important flavor compound but also impairs cell growth severely, which in turn blocks its bioproduction. However, the molecular mechanism of 2-PE tolerance is unclear. In this study, a superb 2-PE stress-tolerant and producing yeast, Candida glycerinogenes, was selected to uncover the unde...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Zhang Z,Lu X,Zong H,Zhuge B

更新日期：2020-05-01 00:00:00

abstract：:Bovine mastitis results in billion dollar losses annually in the USA alone. Streptococci are among the most relevant causative agents of this disease. Conventional antibiotic therapy is often unsuccessful and contributes to development of antibiotic resistance. Bacteriophage endolysins represent a new class of antimic...

journal_title：Applied microbiology and biotechnology

authors： Schmelcher M,Powell AM,Camp MJ,Pohl CS,Donovan DM

更新日期：2015-10-01 00:00:00

abstract：:Organophosphate hydrolase containing hexahistidine tag at the N-terminus of recombinant protein (His6-OPH) and expressed in Escherichia coli cells was purified using supermacroporous polyacrylamide-based monolith columns with immobilized metal affinity matrices [Me2+-iminodiacetic acid (IDA)-polyacrylamide cryogel (PA...

journal_title：Applied microbiology and biotechnology

authors： Efremenko E,Votchitseva Y,Plieva F,Galaev I,Mattiasson B

更新日期：2006-05-01 00:00:00

abstract：:Using a reverse-transcription polymerase chain reaction (RT-PCR) technique, cytochrome P450 genes were cloned from the lignin-degrading basidiomycete, Coriolus versicolor. One possible P450 gene was identified, which consisted of 1,672 nucleotides and a poly(A) tail and encoded a deduced protein containing 449 amino a...

journal_title：Applied microbiology and biotechnology

authors： Ichinose H,Wariishi H,Tanaka H

更新日期：2002-01-01 00:00:00

abstract：:Acrylic polymers (AP) are a diverse group of materials with broad applications, frequent use, and increasing demand. Some of the most used AP are polyacrylamide, polyacrylic acid, polymethyl methacrylates, and polyacrylonitrile. Although no information for the production of all AP types is published, data for the most...

journal_title：Applied microbiology and biotechnology

authors： Gaytán I,Burelo M,Loza-Tavera H

更新日期：2021-01-11 00:00:00

abstract：:Immobilization has been reported as an efficient technique to address the bacterial vulnerability for application in bio self-healing concrete. In this study, for the first time, magnetic iron oxide nanoparticles (IONs) are being practically employed as the protective vehicle for bacteria to evaluate the self-healing ...

journal_title：Applied microbiology and biotechnology

authors： Seifan M,Sarmah AK,Ebrahiminezhad A,Ghasemi Y,Samani AK,Berenjian A

更新日期：2018-03-01 00:00:00

abstract：:The effects of mitomycin C on C1857-controlled recombinant gene expression have been explored in E. coli cultures when the drug was added simultaneously to the thermal induction. A significantly improved yield of homologous, heterologous and chimeric fusion proteins was observed in E. coli MC1061 and GE864 (a MC4100 d...

journal_title：Applied microbiology and biotechnology

authors： Corchero JL,Carbonell X,Benito A,Villaverde A

更新日期：1995-03-01 00:00:00

abstract：:Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may pro...

journal_title：Applied microbiology and biotechnology

authors： Silva ALP,da Silva Caridade TN,Magalhães RR,de Sousa KT,de Sousa CC,Vale JA

更新日期：2020-10-01 00:00:00

abstract：:Lethal effects of pulsed electric fields (PEF) on suspensions of various bacteria, yeast, and spores in buffer solutions and liquid foodstuffs were examined. Living-cell counts of vegetative cell types were reduced by PEF treatment by up to more than four orders of magnitude (> 99.99%). On the other hand, endo- and as...

journal_title：Applied microbiology and biotechnology

authors： Grahl T,Märkl H

更新日期：1996-03-01 00:00:00

abstract：:The interest in industrial biotechnology and its importance opens up challenging possibilities of research in this area. Surfactants have long been among the most versatile of process chemicals. Their market is extremely competitive and manufacturers will have to expand their arsenal to develop products for the year 2...

journal_title：Applied microbiology and biotechnology

authors： Cameotra SS,Makkar RS

更新日期：1998-11-01 00:00:00

abstract：:Panax ginseng C.A. Meyer (ginseng) is a well-known medicinal plant that has been traditionally used in the oriental countries for centuries. Wild ginseng is a scarce and rare commodity. Field cultivation of the ginseng plant is a time-consuming and labor-intensive process. Ginsenosides, a group of glycosylated triterp...

journal_title：Applied microbiology and biotechnology

authors： Murthy HN,Georgiev MI,Kim YS,Jeong CS,Kim SJ,Park SY,Paek KY

更新日期：2014-01-01 00:00:00

abstract：:Ammonium can be removed as dinitrogen gas by cooperating aerobic and anaerobic ammonium-oxidizing bacteria (AerAOB and AnAOB). The goal of this study was to verify putative mutual benefits for aggregated AerAOB and AnAOB in a stagnant freshwater environment. In an ammonium fed water column, the biological oxygen consu...

journal_title：Applied microbiology and biotechnology

authors： Vlaeminck SE,Dierick K,Boon N,Verstraete W

更新日期：2007-07-01 00:00:00

abstract：:Nutrition-based strategies to optimize xylose to ethanol conversion by Pichia stipitis were identified in growing and stationary-phase cultures provided with a defined medium varied in nitrogen, vitamin, purine/pyrimidine, and mineral content via full or partial factorial designs. It is surprising to note that station...

journal_title：Applied microbiology and biotechnology

authors： Slininger PJ,Dien BS,Gorsich SW,Liu ZL

更新日期：2006-10-01 00:00:00

abstract：:Flow cytometry is a rapid and quantitative method to determine bacterial viability. Although different stains can be used to establish viability, staining protocols are inconsistent and lack a general optimization approach. Very few "true" multicolor protocols, where dyes are combined in one sample, have been develope...

journal_title：Applied microbiology and biotechnology

authors： Buysschaert B,Byloos B,Leys N,Van Houdt R,Boon N

更新日期：2016-11-01 00:00:00

abstract：:Carvone, the principal component of spearmint oil, induces biodegradation of polychlorinated biphenyls (PCB) by Arthrobacter sp. strain B1B. This study investigated the effectiveness of the repeated application of carvone-induced bacteria for bioremediation of Aroclor-1242-contaminated soil. Control treatments compare...

journal_title：Applied microbiology and biotechnology

authors： Gilbert ES,Crowley DE

更新日期：1998-10-01 00:00:00

abstract：:ω-Transaminase (ω-TA) is an attractive alternative to metal catalysts for the stereoselective amination of prochiral ketones. The narrow substrate scope of an R-ω-transaminase from Mycobacterium vanbaalenii (MvTA) limits its application in R-amine synthesis. A fluorescence-based TA activity screening system was develo...

journal_title：Applied microbiology and biotechnology

authors： Cheng F,Chen XL,Xiang C,Liu ZQ,Wang YJ,Zheng YG

更新日期：2020-04-01 00:00:00

abstract：:α-Galacto-oligosaccharides (α-GOS) are produced by transgalactosylation reactions of α-galactosidase (α-Gal) or by conversion of raffinose family oligosaccharides by levansucrase. Similarly to β-GOS, α-GOS have the potential to mimic glycan receptors on eukaryotic cells and act as molecular decoys to prevent bacterial...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Black BA,Curtis JM,Gänzle MG

更新日期：2014-03-01 00:00:00

abstract：:A newly isolated strain of Cunninghamella echinulata grown on glucose produced significant quantities of biomass and cellular lipids in media with high C/N ratio. The oil yield from glucose consumed increased after nitrogen exhaustion in the growth medium, but gamma-linolenic acid (GLA) content in cellular oil systema...

journal_title：Applied microbiology and biotechnology

authors： Gema H,Kavadia A,Dimou D,Tsagou V,Komaitis M,Aggelis G

更新日期：2002-03-01 00:00:00

abstract：:Heterologous biosynthesis of tetrahydrocannabinolic acid (THCA) in yeast is a biotechnological process in Natural Product Biotechnology that was recently introduced. Based on heterologous genes from Cannabis sativa and Streptomyces spp. cloned into Saccharomyces cerevisiae, the heterologous biosynthesis was fully embe...

journal_title：Applied microbiology and biotechnology

authors： Thomas F,Schmidt C,Kayser O

更新日期：2020-11-01 00:00:00

abstract：:Copper (Cu(II)) and nickel (Ni(II)) are often encountered in wastewaters. This study investigated the individual toxic effects of long-term addition of Cu(II) and Ni(II) on the biochemical properties of aerobic granules in sequencing batch reactors (SBRs). The biochemical properties of aerobic granules were characteri...

journal_title：Applied microbiology and biotechnology

authors： Wang XH,Gai LH,Sun XF,Xie HJ,Gao MM,Wang SG

更新日期：2010-05-01 00:00:00

abstract：:In order for microalgae-based processes to reach commercial production for biofuels and high-value products such as omega-3 fatty acids, it is necessary that economic feasibility be demonstrated at the industrial scale. Therefore, process optimization is critical to ensure that the maximum yield can be achieved from t...

journal_title：Applied microbiology and biotechnology

authors： Lowrey J,Armenta RE,Brooks MS

更新日期：2016-02-01 00:00:00

abstract：:Sugar beet pulp is a natural carbon source composed mainly of pectin and cellulose, which is utilized and degraded by the ascomycete Penicillium purpurogenum. The fungus also grows on and degrades acetylated xylan which lacks cellulose and pectin. Both carbon sources have been used in our laboratory to grow the fungus...

journal_title：Applied microbiology and biotechnology

authors： Navarrete M,Callegari E,Eyzaguirre J

更新日期：2012-01-01 00:00:00

abstract：:Thioredoxin is crucial for the maintenance of the redox status of cells of all types. Mammalian thioredoxin is secreted from various types of cells, although the mechanism underlying has not yet been clarified. Previously, we demonstrated that thioredoxin was released from Saccharomyces cerevisiae after treatment with...

journal_title：Applied microbiology and biotechnology

authors： Takeuchi Y,Nomura W,Ohdate T,Tamasu S,Masutani H,Murata K,Izawa S,Yodoi J,Inoue Y

更新日期：2007-07-01 00:00:00

abstract：:Guanosine 5'-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5'-diphosphate (GDP)-L-fucose. In this study, improvement of GDP-L-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-L-fucose. The effects of...

journal_title：Applied microbiology and biotechnology

authors： Lee WH,Shin SY,Kim MD,Han NS,Seo JH

更新日期：2012-03-01 00:00:00