Characterization of the metabolic shift of Saccharomyces bayanus var. uvarum by continuous aerobic culture.

abstract：:Many hydrocarbon-contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, high salt concentrations, or high pressure, Hydrocarbon-degrading microorganisms, adapted to grow and thrive in these environments, play an important role in the biological treatment of polluted extreme...

journal_title：Applied microbiology and biotechnology

authors： Margesin R,Schinner F

更新日期：2001-09-01 00:00:00

abstract：:Extracellular polymeric substances (EPS) play an important role in the formation and activity of biofilms in wastewater treatment (WWT). The EPS of the denitrifying biomarker Comamonas denitrificans strain 110, produced in different culture media and growth modes, were characterized. The EPS mainly contained protein (...

journal_title：Applied microbiology and biotechnology

authors： Andersson S,Dalhammar G,Land CJ,Kuttuva Rajarao G

更新日期：2009-03-01 00:00:00

abstract：:Geminiviruses have evolved with tremendous potential of recombination and possess the ability to manipulate several cellular processes of hosts. Chilli leaf curl virus (ChiLCV) is a monopartite Begomovirus (family Geminiviridae) which has emerged as a serious threat to chilli production worldwide. To date, development...

journal_title：Applied microbiology and biotechnology

authors： Kushwaha N,Sahu PP,Prasad M,Chakraborty S

更新日期：2015-06-01 00:00:00

abstract：:Laccases are blue multicopper oxidases with potential applications in environmental and industrial biotechnology. In this study, a new bacterial laccase gene of 1.32 kb was obtained from a marine microbial metagenome of the South China Sea by using a sequence screening strategy. The protein (named as Lac15) of 439 ami...

journal_title：Applied microbiology and biotechnology

authors： Fang Z,Li T,Wang Q,Zhang X,Peng H,Fang W,Hong Y,Ge H,Xiao Y

更新日期：2011-02-01 00:00:00

abstract：:Bacillus subtilis 916 was an effective biocontrol agent in control rice sheath blight caused by Rhizoctonia solani. To further improve its antagonistic ability, low-energy ion implantation was applied in Bs-916. We studied the effects of different doses of N(+) implantation. The optimum dose of ion implantation for th...

journal_title：Applied microbiology and biotechnology

authors： Li D,Nie F,Wei L,Wei B,Chen Z

更新日期：2007-07-01 00:00:00

abstract：:Cytophaga hutchinsonii is a Gram-negative aerobic soil bacterium which can digest crystalline cellulose completely through a strategy different from that of the well-studied cellulolytic aerobic fungi and anaerobic bacteria. However, despite the availability of a published genome sequence, studies on this organism hav...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Ji X,Chen N,Li P,Liu W,Lu X

更新日期：2012-01-01 00:00:00

abstract：:Recently, a new gene encoding beta-glucuronidase from Streptococcus equi subsp. zooepidemicus (SEZ) was identified and expressed in Escherichia coli. In this paper, the characterization of the enzyme is described. Specific enzyme activity was 120,000 U/mg purified protein at 37 degrees C and pH = 7.0. The temperature ...

journal_title：Applied microbiology and biotechnology

authors： Krahulec J,Krahulcová J

更新日期：2007-04-01 00:00:00

abstract：:Severe dengue is more likely found during secondary heterologous dengue virus (DENV) infection or primary infection of infants born to dengue-immune mothers and led to the hypothesis of antibody-dependent enhancement (ADE). It has been reported that pre-membrane (prM)-reactive antibodies do not efficiently neutralize ...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Si L,Luo Y,Guo X,Zhou J,Fang D,Yan H,Zeng G,Jiang L

更新日期：2015-11-01 00:00:00

abstract：:A novel and efficient screening method for pyrroloquinoline quinone (PQQ) high-yielding methylotrophic strains was developed by using glucose dehydrogenase apoenzyme (GDHA) which depended on PQQ as the cofactor. Using this high-throughput method, PQQ high-yielding strains were rapidly screened out from thousands of me...

journal_title：Applied microbiology and biotechnology

authors： Si Z,Zhu J,Wang W,Huang L,Wei P,Cai J,Xu Z

更新日期：2016-12-01 00:00:00

abstract：:Two cephalosporin genes from Acremonium chrysogenum, pcbAB and pcbC encode the ACV (alpha-aminoadipyl-cysteinyl-valine) synthetase and isopenicillin N-synthetase, respectively. The two adjacent genes are orientated in opposite directions on the chromosomal DNA, separated by a 1.2-kb non-translated sequence, carrying t...

journal_title：Applied microbiology and biotechnology

authors： Menne S,Walz M,Kück U

更新日期：1994-10-01 00:00:00

abstract：:The paper describes the preparation of new probiotic formulations based on chitosan-coated alginate microcapsules containing three different probiotic strains, Lactobacillus plantarum PBS067, Lactobacillus rhamnosus PBS070, and Bifidobacterium animalis subsp. lactis PBS075 taken individually and as a mixture of them. ...

journal_title：Applied microbiology and biotechnology

authors： D'Orazio G,Di Gennaro P,Boccarusso M,Presti I,Bizzaro G,Giardina S,Michelotti A,Labra M,La Ferla B

更新日期：2015-11-01 00:00:00

abstract：:A recombinant form of the sweet-tasting protein thaumatin has been produced in the filamentous fungus Aspergillus niger var. awamori. Expression cassettes containing a synthetic gene encoding thaumatin II were prepared and used to transform Aspergillus niger var. awamori strain NRRL312. Several fungal strains capable ...

journal_title：Applied microbiology and biotechnology

authors： Faus I,del Moral C,Adroer N,del Río JL,Patiño C,Sisniega H,Casas C,Bladé J,Rubio V

更新日期：1998-04-01 00:00:00

abstract：:A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M....

journal_title：Applied microbiology and biotechnology

authors： Li Z,Geng M,Yang H

更新日期：2015-01-01 00:00:00

abstract：:Alternative ligands such as nucleic acid aptamers can be used for pathogen capture and detection and offer advantages over antibodies, including reduced cost, ease of production and modification, and improved stability. DNA aptamers demonstrating binding specificity to Salmonella enterica serovar Typhimurium were iden...

journal_title：Applied microbiology and biotechnology

authors： Dwivedi HP,Smiley RD,Jaykus LA

更新日期：2013-04-01 00:00:00

abstract：:Phytophthora infestans is the causative agent of potato blight that resulted in the great famine in Ireland in the nineteenth century. This microbe can release large amounts of the C20 very long-chain polyunsaturated fatty acids arachidonic acid (ARA; 20:4Δ(5, 8, 11, 14)) and eicosapentaenoic acid (EPA; 20:5Δ(5, 8, 11...

journal_title：Applied microbiology and biotechnology

authors： Sun Q,Liu J,Zhang Q,Qing X,Dobson G,Li X,Qi B

更新日期：2013-09-01 00:00:00

abstract：:The tandem multimeric expression of various peptides has been explored by many researchers. However, expression levels have usually not been proportional to the degree of multimerization. To increase the expression level in Escherichia coli of tandem multimers of a cationic antimicrobial peptide, buforin II, fused to ...

journal_title：Applied microbiology and biotechnology

authors： Lee JH,Kim MS,Cho JH,Kim SC

更新日期：2002-05-01 00:00:00

abstract：:A strong promoter increases transcription of the genes of interest and enhances the production of various valuable substances. For a hyperthermophilic archaeon Thermococcus onnurineus NA1, which can produce H2 from carbon monoxide oxidation, we searched for a novel endogenous strong promoter by transcriptome analysis ...

journal_title：Applied microbiology and biotechnology

authors： Lee SH,Kim MS,Jung HC,Lee J,Lee JH,Lee HS,Kang SG

更新日期：2015-05-01 00:00:00

abstract：:Myogenesis involves the determination of progenitor cells to myoblasts, their fusion to yield multinuclear myotubes, and the maturation of myotubes to muscle fibres. This development is reflected in a time pattern of gene expression, e.g. of genes coding for desmin, the myogenic factors myogenin and myoD, the acetylch...

journal_title：Applied microbiology and biotechnology

authors： Bardouille C,Lehmann J,Heimann P,Jockusch H

更新日期：2001-05-01 00:00:00

abstract：:Because engineering of the 101.016-bp megaplasmid pKB1 of Gordonia westfalica Kb1 failed due to the absence of an effective transfer system, pKB1 was transferred by conjugation from G. westfalica Kb1 to a kanamycin-resistant mutant of Rhodococcus opacus PD630 at a frequency of about 6.2 x 10(-8) events per recipient c...

journal_title：Applied microbiology and biotechnology

authors： Bröker D,Arenskötter M,Steinbüchel A

更新日期：2008-01-01 00:00:00

abstract：:Listeria monocytogenes exhibits symbiotic codependence with the dominant commensal bacteria, which may help it avoid being removed or inactivated by disinfectants in local environments. In this study, we investigated L. monocytogenes-positive biofilms at food production facilities, and the dominant bacterial species o...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Zhang H,Wu C,Deng W,Wang D,Zhao G,Song J,Jiang Y

更新日期：2016-04-01 00:00:00

abstract：:The impact of dibenzofuran (DF) and dibenzo-p-dioxin (DD) on the changes in bacterial community structure and the transition of catabolic genes were studied using forest soil. The bacterial community structure of soil suspensions amended with 1 microg/g of either DF or DD was analyzed by 16S rRNA and functional gene s...

journal_title：Applied microbiology and biotechnology

authors： Kimura N,Kamagata Y

更新日期：2009-08-01 00:00:00

abstract：:The internal loop photobiodegradation reactor (ILPBR) was evaluated for the degradation of the pharmaceutical sulfamethoxazole (SMX) using batch experiments following three protocols: photolysis alone (P), biodegradation alone (B), and intimately coupled photolysis and biodegradation (P&B). SMX was removed more rapidl...

journal_title：Applied microbiology and biotechnology

authors： Yan N,Xia S,Xu L,Zhu J,Zhang Y,Rittmann BE

更新日期：2012-04-01 00:00:00

abstract：:The broad-spectrum organophosphate hydrolase (OPH; EC 3.1.8.1) encoded by the organophosphate-degrading gene (opd) from Pseudomonas diminuta MG and Flavobacterium sp. ATCC 27551 possesses capabilities of both P-O bond hydrolysis (e.g. paraoxon) and P-F bond hydrolysis [e.g. sarin and diisopropylfluorophosphate (DFP)]....

journal_title：Applied microbiology and biotechnology

authors： Dave KI,Lauriano C,Xu B,Wild JR,Kenerley CM

更新日期：1994-05-01 00:00:00

abstract：:Batch experiments were conducted to assess the biotransformation potential of four hydrocarbon monoterpenes (d-limonene, alpha-pinene, gamma-terpinene, and terpinolene) and four alcohols (arbanol, linalool, plinol, and alpha-terpineol) under aerobic conditions at 23 degrees C. Both forest-soil extract and enriched cul...

journal_title：Applied microbiology and biotechnology

authors： Misra G,Pavlostathis SG,Perdue EM,Araujo R

更新日期：1996-07-01 00:00:00

abstract：:Anaerobic biodegradation of atrazine by the bacterial isolate M91-3 was characterized with respect to mineralization, metabolite formation, and denitrification. The ability of the isolate to enhance atrazine biodegradation in anaerobic sediment slurries was also investigated. The organism utilized atrazine as its sole...

journal_title：Applied microbiology and biotechnology

authors： Crawford JJ,Sims GK,Mulvaney RL,Radosevich M

更新日期：1998-05-01 00:00:00

abstract：:The goal of modern taxonomy is to understand the relationships of living organisms in terms of evolutionary descent. Thereby, the relationships between living organisms are understood in terms of nested clades--every time a speciation event takes place, two new clades are produced. Life comprises three domains of livi...

journal_title：Applied microbiology and biotechnology

authors： Voigt K,Kirk PM

更新日期：2011-04-01 00:00:00

abstract：:The production of botulinum neurotoxin A (BoNT/A) for therapeutic and cosmetic applications requires precise determination of batch potency, and the enzymatic activity of BoNT/A light chain is a crucial index that can be measured in vitro. We previously established a SNAP-25 chip-based assay using surface plasmon reso...

journal_title：Applied microbiology and biotechnology

authors： Lévêque C,Ferracci G,Maulet Y,Grand-Masson C,Seagar M,El Far O

更新日期：2015-05-01 00:00:00

abstract：:A gene for a synthetic protein-based polymer, G-(VPGVG)119-VPGV, coding for the EG-120mer (elastomer), was cloned into a fungal expression vector to allow constitutive expression of the polymer controlled by the gpdA (glyceraldehyde-3-phosphate dehydrogenase) promoter sequence of Aspergillus nidulans. Stable transform...

journal_title：Applied microbiology and biotechnology

authors： Herzog RW,Singh NK,Urry DW,Daniell H

更新日期：1997-04-01 00:00:00

abstract：:The lignin-degrading, biopulping white-rot fungus Physisporinus rivulosus secretes several laccases of distinct features such as thermostability, extremely low pH optima and thermal activation for oxidation of phenolic substrates. Here we describe the cloning, heterologous expression and structural and enzymatic chara...

journal_title：Applied microbiology and biotechnology

authors： Hildén K,Mäkelä MR,Lundell T,Kuuskeri J,Chernykh A,Golovleva L,Archer DB,Hatakka A

更新日期：2013-02-01 00:00:00

abstract：:Formate dehydrogenases (FDH) are useful for the regeneration of NADH, which is required for asymmetric reduction by several dehydrogenases and reductases. FDHs have relatively low activity and are labile, especially to alpha-haloketones, thus FDH cannot be applied to the industrial manufacture of optically active alph...

journal_title：Applied microbiology and biotechnology

authors： Yamamoto H,Mitsuhashi K,Kimoto N,Kobayashi Y,Esaki N

更新日期：2005-04-01 00:00:00