Heterologous expression and structural characterization of two low pH laccases from a biopulping white-rot fungus Physisporinus rivulosus.

abstract：:High vanillin productivity was achieved in the batch biotransformation of ferulic acid by Streptomyces sp. strain V-1. Due to the toxicity of vanillin and the product inhibition, fed-batch biotransformation with high concentration of ferulic acid was unsuccessful. To solve this problem and improve the vanillin yield, ...

journal_title：Applied microbiology and biotechnology

authors： Hua D,Ma C,Song L,Lin S,Zhang Z,Deng Z,Xu P

更新日期：2007-03-01 00:00:00

abstract：:Inactivation of D-amino acid oxidase occurred by different mechanisms. The enzyme showed a rapid loss of activity in the presence of micromolar amounts of Cu2+ and Hg2+. It was also sensitive to oxidative inactivation by Fe2+ and H2O2 when both reagents were added in millimolar amounts. When oxidatively inactivated D-...

journal_title：Applied microbiology and biotechnology

authors： Schräder T,Andreesen JR

更新日期：1996-05-01 00:00:00

abstract：:Previous studies have shown that a 20-kDa protein enhances production of the insecticidal CytA and CryIVA proteins of Bacillus thuringiensis in Escherichia coli as well as CytA production and crystal formation in B. thuringiensis. To determine whether the 20-kDa protein could enhance CryIVD production, an expression v...

journal_title：Applied microbiology and biotechnology

authors： Wu D,Federici BA

更新日期：1995-01-01 00:00:00

abstract：:Xylanase is the enzyme complex that is responsible for the degradation of xylan; however, novel xylanase producers remain to be explored in marine environment. In this study, a Streptomyces strain M11 which exhibited xylanase activity was isolated from marine sediment. The 16S rDNA sequence of M11 showed the highest i...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Zhao X,Bai F

更新日期：2013-05-01 00:00:00

abstract：:meta-Cleavage product (MCP) hydrolases can catalyze relatively low reactive carbon-carbon bond hydrolysis of products, which are derived from the meta-cleavage of catechols. The strict substrate selectivity of MCP hydrolases attracts an interest to understand the determinants of substrate specificity. Compared with co...

journal_title：Applied microbiology and biotechnology

authors： Zhou H,Qu Y,Shen E,Kong C,Zhang X,Ma Q,Zhou J

更新日期：2013-06-01 00:00:00

abstract：:Myogenesis involves the determination of progenitor cells to myoblasts, their fusion to yield multinuclear myotubes, and the maturation of myotubes to muscle fibres. This development is reflected in a time pattern of gene expression, e.g. of genes coding for desmin, the myogenic factors myogenin and myoD, the acetylch...

journal_title：Applied microbiology and biotechnology

authors： Bardouille C,Lehmann J,Heimann P,Jockusch H

更新日期：2001-05-01 00:00:00

abstract：:Biocatalytic asymmetric preparation of (R)-1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent (R)-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on m...

journal_title：Applied microbiology and biotechnology

authors： Zheng RC,Ge Z,Qiu ZK,Wang YS,Zheng YG

更新日期：2012-05-01 00:00:00

abstract：:The active efflux system contributing to the solvent tolerance of Pseudomonas putida S12 was characterized physiologically. The mutant P. putida JK1, which lacks the active efflux system, was compared with the wild-type organism. None of 20 known substrates of common multi-drug-resistant pumps had a stronger growth-in...

journal_title：Applied microbiology and biotechnology

authors： Isken S,De Bont JA

更新日期：2000-11-01 00:00:00

abstract：:Saccharomyces bayanus, being of interest for wine-making, is not as well known as S. cerevisiae and, due to many changes in the yeast classification, accurate data concerning its metabolic activity are difficult to find. In order to produce this yeast as an active dry yeast to be used as a starter in wine-making, its ...

journal_title：Applied microbiology and biotechnology

authors： Serra A,Strehaiano P,Taillandier P

更新日期：2003-10-01 00:00:00

abstract：:Rapid sugar consumption is important for the microbial production of chemicals and fuels. Here, we show that overexpression of the NADH dehydrogenase gene (ndh) increased glucose consumption rate in Corynebacterium glutamicum under oxygen-deprived conditions through investigating the relationship between the glucose c...

journal_title：Applied microbiology and biotechnology

authors： Tsuge Y,Uematsu K,Yamamoto S,Suda M,Yukawa H,Inui M

更新日期：2015-07-01 00:00:00

abstract：:Photothermal cancer therapy, as a prospective approach for local cancer treatment, is attracting increasing interests. In this paper, gold nanorods were conjugated with folate (folate/AuNRs), and their photothermal effects on hepatocellular carcinoma cell line (HepG2) using MTT assay, flow cytometry, as well as on the...

journal_title：Applied microbiology and biotechnology

authors： Jin H,Yang P,Cai J,Wang J,Liu M

更新日期：2012-06-01 00:00:00

abstract：:Palm-oil mill fiber (POMF) is a fibrous, natural hard material discharged in enormous amounts from palm-oil mills in tropical plantations; therefore, research to find microorganisms that decompose POMF was conducted. As the result of screening, a new thermophilic fungus, Chaetomium sp. nov. MS-017, exhibiting rapid gr...

journal_title：Applied microbiology and biotechnology

authors： Suyanto,Ohtsuki T,Yazaki S,Ui S,Mimura A

更新日期：2003-01-01 00:00:00

abstract：:Ethidium monoazide bromide (EMA) treatment of pure culture and environmental waters at low concentrations (1.0-7.5 microg/ml) indicated effective enumeration of viable and viable but nonculturable Escherichia coli in pure cultures, creek waters, and secondary activated sludge effluent samples by quantitative polymeras...

journal_title：Applied microbiology and biotechnology

authors： Gedalanga PB,Olson BH

更新日期：2009-03-01 00:00:00

abstract：:Hyaluronic acid (HA) is a ubiquitous polysaccharide found in humans, animals, bacteria, algae and molluscs. Simple yet sophisticated, HA demonstrates unique and valuable rheological properties. In solution, HA behaves as a stiffened random coil and the resultant behaviour, even at low concentrations, is far from Newto...

journal_title：Applied microbiology and biotechnology

authors： Marcellin E,Steen JA,Nielsen LK

更新日期：2014-08-01 00:00:00

abstract：:Bacteria that grow on p-xylene, p-toluic acid, and terephthalic acid (TPA) were isolated from a wastewater bioreactor that is used to treat a waste stream that contains all three of these compounds. Although previously described aerobic bacteria degrade p-xylene by initially oxidizing a single methyl group to form p-t...

journal_title：Applied microbiology and biotechnology

authors： Bramucci MG,McCutchen CM,Singh M,Thomas SM,Larsen BS,Buckholz J,Nagarajan V

更新日期：2002-02-01 00:00:00

abstract：:Tacrolimus (FK506), an effective immunosuppressant, is widely used in the treatment of autoimmune diseases. In this study, we identified that BulZ, a Streptomyces antibiotic regulatory protein (SARP) family regulator, acted as a positive regulator for spore differentiation and tacrolimus production. A knockout of bulZ...

journal_title：Applied microbiology and biotechnology

authors： Ma D,Wang C,Chen H,Wen J

更新日期：2018-06-01 00:00:00

abstract：:The unicellular cyanobacterium Synechocystis sp. PCC6714 can grow not only under photoautotrophic conditions, but also under chemoheterotrophic conditions if glucose is added to the medium. This makes it useful for the study of many aspects of bioenergetic mechanisms. In contrast to its closely related strain Synechoc...

journal_title：Applied microbiology and biotechnology

authors： Ludwig A,Heimbucher T,Gregor W,Czerny T,Schmetterer G

更新日期：2008-03-01 00:00:00

abstract：:In response to changing extracellular pH levels, phosphate-limited continuous cultures of Clostridium acetobutylicum reversibly switches its metabolism from the dominant formation of acids to the prevalent production of solvents. Previous experimental and theoretical studies have revealed that this pH-induced metaboli...

journal_title：Applied microbiology and biotechnology

authors： Millat T,Janssen H,Thorn GJ,King JR,Bahl H,Fischer RJ,Wolkenhauer O

更新日期：2013-07-01 00:00:00

abstract：:Aspergillus oryzae is a fungus used extensively in the fermentation industry. We constructed cDNA microarrays comprising 2,070 highly expressed cDNAs selected from the approximately 6,000 non-redundant expressed sequence tags (ESTs) in the A. oryzae EST database (http://www.aist.go.jp/RIODB/ffdb/index.html). Using the...

journal_title：Applied microbiology and biotechnology

authors： Maeda H,Sano M,Maruyama Y,Tanno T,Akao T,Totsuka Y,Endo M,Sakurada R,Yamagata Y,Machida M,Akita O,Hasegawa F,Abe K,Gomi K,Nakajima T,Iguchi Y

更新日期：2004-07-01 00:00:00

abstract：:This review presents developments and applications in bioleaching and mineral biooxidation since publication of a previous mini review in 2003 (Olson et al. Appl Microbiol Biotechnol 63:249-257, 2003). There have been discoveries of newly identified acidophilic microorganisms that have unique characteristics for effec...

journal_title：Applied microbiology and biotechnology

authors： Brierley CL,Brierley JA

更新日期：2013-09-01 00:00:00

abstract：:This study evaluated the effect of inorganic mercury (Hg) on bacterial community and diversity in different soils. Three soils-neutral, alkaline and acidic-were spiked with six different concentrations of Hg ranging from 0 to 200 mg kg-1 and aged for 90 days. At the end of the ageing period, 18 samples from three diff...

journal_title：Applied microbiology and biotechnology

authors： Mahbub KR,Subashchandrabose SR,Krishnan K,Naidu R,Megharaj M

更新日期：2017-03-01 00:00:00

abstract：:Due to the emerging environmental issues related to heavy metals, concern about the soil quality of farming lands near manufacturing district is increasing. Investigating the function of soil microorganisms exposed to long-term heavy metal contamination is meaningful and important for agricultural soil utilization. Th...

journal_title：Applied microbiology and biotechnology

authors： Deng L,Zeng G,Fan C,Lu L,Chen X,Chen M,Wu H,He X,He Y

更新日期：2015-10-01 00:00:00

abstract：:κ-Carrageenases exhibit apparent distinctions in gene sequence, molecular weight, enzyme properties, and posttranslational processes. In this study, a new κ-carrageenase gene named cgkZ was cloned from the marine bacterium Zobellia sp. ZM-2. The gene comprised an open reading frame of 1,638 bp and encoded 545 amino ac...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Li G,Mo Z,Mou H

更新日期：2013-12-01 00:00:00

abstract：:A set of filamentous fungi (42 strains) was screened for alpha-N-acetylgalactosaminidase activity, and a series of inducers and different cultivation conditions were tested. Enzyme production by the best producer Aspergillus niger CCIM K2 was optimized and scaled up. alpha-N-Acetylgalactosaminidase was purified to app...

journal_title：Applied microbiology and biotechnology

authors： Weignerová L,Filipi T,Manglová D,Kren V

更新日期：2008-07-01 00:00:00

abstract：:Analysis of gene expression data related to assimilation and biosynthesis of nitrogen-containing compounds amino acids, peptides, and nucleotides was used to monitor availability of these nutrients to Escherichia coli MG1655 growing on Luria-Bertani medium. The data indicate that free amino acids and nucleotides only ...

journal_title：Applied microbiology and biotechnology

authors： Baev MV,Baev D,Radek AJ,Campbell JW

更新日期：2006-07-01 00:00:00

abstract：:The aim of this study was to discover new nitrilases with useful activities, especially towards dinitriles that are precursors of high-value cyano acids. Genes coding for putative nitrilases of different origins (fungal, plant, or bacterial) with moderate similarities to known nitrilases were selected by mining the Ge...

journal_title：Applied microbiology and biotechnology

authors： Veselá AB,Rucká L,Kaplan O,Pelantová H,Nešvera J,Pátek M,Martínková L

更新日期：2016-03-01 00:00:00

abstract：:We report on a pheromone-based inter-species communication system, allowing for a controlled cell-cell communication between the two species Saccharomyces cerevisiae and Schizosaccharomyces pombe as a proof of principle. It exploits the mating response pathways of the two yeast species employing the pheromones, α- or ...

journal_title：Applied microbiology and biotechnology

authors： Hennig S,Clemens A,Rödel G,Ostermann K

更新日期：2015-02-01 00:00:00

abstract：:Clonorchis sinensis (C. sinensis), an important fishborne zoonotic parasite threatening public health, is of major socioeconomic importance in epidemic areas. Effective strategies are still urgently expected to prevent against C. sinensis infection. In the present study, paramyosin of C. sinensis (CsPmy) was stably an...

journal_title：Applied microbiology and biotechnology

authors： Sun H,Shang M,Tang Z,Jiang H,Dong H,Zhou X,Lin Z,Shi C,Ren P,Zhao L,Shi M,Zhou L,Pan H,Chang O,Li X,Huang Y,Yu X

更新日期：2020-02-01 00:00:00

abstract：:Triphenylmethane dyes are extensively utilized in textile industries, medicinal products, biological stains, and food processing industries, etc. They are generally considered as xenobiotic compounds, which are very recalcitrant to biodegradation. The widespread persistence of such compounds has generated concerns wit...

journal_title：Applied microbiology and biotechnology

authors： Fu XY,Zhao W,Xiong AS,Tian YS,Zhu B,Peng RH,Yao QH

更新日期：2013-02-01 00:00:00

abstract：:Whey, the main by-product of the cheese industry, is considered as an important pollutant due to its high chemical and biological oxygen demand. Whey, often considered as waste, has high nutritional value and can be used to obtain value-added products, although some of them need expensive enzymatic synthesis. An econo...

journal_title：Applied microbiology and biotechnology

authors： Pescuma M,de Valdez GF,Mozzi F

更新日期：2015-08-01 00:00:00