Heterologous signal peptides-directing secretion of Streptomyces mobaraensis transglutaminase by Bacillus subtilis.

abstract：:2-Phenylethanol (2-PE) is an important flavor compound but also impairs cell growth severely, which in turn blocks its bioproduction. However, the molecular mechanism of 2-PE tolerance is unclear. In this study, a superb 2-PE stress-tolerant and producing yeast, Candida glycerinogenes, was selected to uncover the unde...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Zhang Z,Lu X,Zong H,Zhuge B

更新日期：2020-05-01 00:00:00

abstract：:The microbial oxidation of ebastine to carebastine was investigated. Among the 15 micro-organisms examined, only the Cunninghamella strains showed the desired biotransformation. Cunninghamella blakesleeana oxidised the substrate within 7 days, via the intermediates alcohol and aldehyde, mainly to carebastine, the corr...

journal_title：Applied microbiology and biotechnology

authors： Schwartz H,Liebig-Weber A,Hochstätter H,Böttcher H

更新日期：1996-02-01 00:00:00

abstract：:At present, more than 100 strains of Lentinula edodes are cultivated on a commercial scale in China. A simple, reliable, and effective method to distinguish some commercial strains of the superior type from other commercial strains is very important for the Lentinula industry. In this study, 23 commercial strains of L...

journal_title：Applied microbiology and biotechnology

authors： Li HB,Wu XQ,Peng HZ,Fu LZ,Wei HL,Wu QQ,Jin QY,Li N

更新日期：2008-11-01 00:00:00

abstract：:Stirred, pH-controlled anaerobic batch cultures were used to evaluate the in vitro utilisation by canine gut microflora of novel alpha-galactooligosaccharides synthesised with an enzyme extract from a canine Lactobacillus reuteri strain. Fructooligosaccharides (FOS), melibiose and raffinose were used as reference carb...

journal_title：Applied microbiology and biotechnology

authors： Tzortzis G,Goulas AK,Baillon ML,Gibson GR,Rastall RA

更新日期：2004-03-01 00:00:00

abstract：:Lyme borreliosis is a bacterial infection that can be spread to humans by infected ticks and may severely affect many organs and tissues. Nearly four decades have elapsed since the discovery of the disease agent called Borrelia burgdorferi. Although there is a plethora of knowledge on the infectious agent and thousand...

journal_title：Applied microbiology and biotechnology

authors： Strnad M,Grubhoffer L,Rego ROM

更新日期：2020-03-01 00:00:00

abstract：:Sulfadiazine (SDZ) is an antibiotic frequently administered to livestock, and it alters microbial communities when entering soils with animal manure, but understanding the interactions of these effects to the prevailing climatic regime has eluded researchers. A climatic factor that strongly controls microbial activity...

journal_title：Applied microbiology and biotechnology

authors： Reichel R,Radl V,Rosendahl I,Albert A,Amelung W,Schloter M,Thiele-Bruhn S

更新日期：2014-01-01 00:00:00

abstract：:The Escherichia coli (E. coli) expression system has been widely used to produce recombinant proteins. However, in some heterologous expressions, there are still difficulties in large-scale production. The use of fusion partners is one of the strategies for improving the expression levels of proteins in E. coli host. ...

journal_title：Applied microbiology and biotechnology

authors： Nguyen TKM,Ki MR,Son RG,Pack SP

更新日期：2019-03-01 00:00:00

abstract：:A cDNA encoding a bifunctional acetylxylan esterase/xylanase, XynS20E, was cloned from the ruminal fungus Neocallimastix patriciarum. A putative conserved domain of carbohydrate esterase family 1 was observed at the N-terminus and a putative conserved domain of glycosyl hydrolase family 11 was detected at the C-termin...

journal_title：Applied microbiology and biotechnology

authors： Pai CK,Wu ZY,Chen MJ,Zeng YF,Chen JW,Duan CH,Li ML,Liu JR

更新日期：2010-02-01 00:00:00

abstract：:Residual biomass, produced by the thermophilic fungus, Talaromyces emersonii CBS 814.70, following growth on glucose-containing media, was examined for its ability to take up uranium from aqueous solution. It was found that the biomass had a relatively high observed biosorption capacity for the uranium (280 mg/g dry w...

journal_title：Applied microbiology and biotechnology

authors： Bengtsson L,Johansson B,Hackett TJ,McHale L,McHale AP

更新日期：1995-01-01 00:00:00

abstract：:Hydroxy fatty acids (HFA) have gained importance because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. The bacterial isolate Pseudomonas aeruginosa (PR3) was reported to produce mono-, di-, and trihydroxy fatty acids from different unsaturated fatty ac...

journal_title：Applied microbiology and biotechnology

authors： Chang IA,Kim IH,Kang SC,Hou CT,Kim HR

更新日期：2007-02-01 00:00:00

abstract：:Internal fragments of alpha- and beta-tubulin genes were generated using reverse transcription polymerase chain reaction (RT-PCR), and the termini were isolated using 5'- and 3'-rapid amplification of cDNA ends. Phytophthora capsici alpha- and beta-tubulin specific primers were then used to generate full-length cDNA b...

journal_title：Applied microbiology and biotechnology

authors： Koo BS,Park H,Kalme S,Park HY,Han JW,Yeo YS,Yoon SH,Kim SJ,Lee CM,Yoon MY

更新日期：2009-03-01 00:00:00

abstract：:The paper describes the synthesis of the phosphorylcholine-binding miniantibody McPC603scFvDhl x in cell-wall-less L-form strains of Escherichia coli and Proteus mirabilis. Cells of these strains were transformed with the plasmid pACK02scKan, carrying the miniantibody (miniAb) coding sequence under the control of the ...

journal_title：Applied microbiology and biotechnology

authors： Kujau MJ,Hoischen C,Riesenberg D,Gumpert J

更新日期：1998-01-01 00:00:00

abstract：:Intervention strategies for obesity are global issues that require immediate attention. The objective of this study was to assess the possibility that Clostridium butyricum and its potential components could reduce lipogenesis. Co-culture experiments of Caco-2 cells and 1 × 10(6), 1 × 10(7), and 1 × 10(8) CFU/ml of C....

journal_title：Applied microbiology and biotechnology

authors： Zhao X,Guo Y,Liu H,Gao J,Nie W

更新日期：2014-09-01 00:00:00

abstract：:The biomass carbon ratios of biochemicals related to biomass have been reviewed. Commercial products from biomass were explained. The biomass carbon ratios of biochemical compounds were measured by accelerator mass spectrometry (AMS) based on the (14)C concentration of carbons in the compounds. This measuring method u...

journal_title：Applied microbiology and biotechnology

authors： Kunioka M

更新日期：2010-06-01 00:00:00

abstract：:Two recombinant Aeropyrum pernix glutamate dehydrogenase (GDH) enzymes with different length N-termini were cloned and expressed in Escherichia coli: sGDH begins with the amino acid sequence of the extracted native enzyme (M-Q-P-T-D-P-L-E-E), whereas lGDH begins with the sequence of the predicted ORF (M-E-V-L-A-L-Q-P-...

journal_title：Applied microbiology and biotechnology

authors： Helianti I,Morita Y,Murakami Y,Yokoyama K,Tamiya E

更新日期：2002-08-01 00:00:00

abstract：:The effect of the presence of supplementary glucose or acetate on the growth and pyridine-degrading activity of freely suspended and calcium-alginate-immobilized Pimelobacter sp. was investigated. Although the supplementary carbon sources could be degraded simultaneously with pyridine, Pimelobacter sp. exhibited a pre...

journal_title：Applied microbiology and biotechnology

authors： Rhee SK,Lee GM,Lee ST

更新日期：1996-02-01 00:00:00

abstract：:Media and growth conditions were optimized for the microaerobic cultivation of Magnetospirillum gryphiswaldense in flasks and in a fermentor, resulting in significantly increased cell and magnetosome yields, compared with earlier studies. A reliable method was established for the automatic control of low dissolved oxy...

journal_title：Applied microbiology and biotechnology

authors： Heyen U,Schüler D

更新日期：2003-06-01 00:00:00

abstract：:Alcaligenes sp. A 7-2 immobilized on granular clay has been applied in a percolator to degrade 4-chlorophenol in sandy soil. Good adsorption rates on granular clay were achieved using cell suspensions with high titres and media at pH 8.0. The influence of various parameters such as aeration rate, pH, temperature, conc...

journal_title：Applied microbiology and biotechnology

authors： Balfanz J,Rehm HJ

更新日期：1991-08-01 00:00:00

abstract：:Dramatic increase in bacterial resistance towards conventional antibiotics emphasises the importance to identify novel, more potent antimicrobial therapies. Antimicrobial peptides (AMPs) have emerged as a promising new group to be evaluated in therapeutic intervention of infectious diseases. Here we describe a novel A...

journal_title：Applied microbiology and biotechnology

authors： Myhrman E,Håkansson J,Lindgren K,Björn C,Sjöstrand V,Mahlapuu M

更新日期：2013-04-01 00:00:00

abstract：:Mitigation of the methane (CH4) emission from ruminants is needed to decrease the environmental impact of ruminant animal production. Different plant materials and chemicals have been tested, but few are both effective and practical. Medicinal herbs contain biological compounds and antimicrobials that may be effective...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Liu M,Wu Y,Wang L,Liu J,Jiang L,Yu Z

更新日期：2016-11-01 00:00:00

abstract：:Trehalose (α-D-glucopyranosyl-(1 → 1)-α-D-glucopyranoside) is a non-reducing disaccharide composed of two glucose molecules linked by an α,α-1,1-glycosidic bond. It possesses physicochemical properties, which account for its biological roles in a variety of prokaryotic and eukaryotic organisms and invertebrates. Inten...

journal_title：Applied microbiology and biotechnology

authors： Cai X,Seitl I,Mu W,Zhang T,Stressler T,Fischer L,Jiang B

更新日期：2018-04-01 00:00:00

abstract：:Aspartic proteases are a relatively small group of proteolytic enzymes that are active in acidic environments and are found across all forms of life. Certain microorganisms secrete such proteases as virulence agents and/or in order to break down proteins thereby liberating assimilable sources of nitrogen. Some of the ...

journal_title：Applied microbiology and biotechnology

authors： Theron LW,Divol B

更新日期：2014-11-01 00:00:00

abstract：:In this study, the kinetic behaviors between acetone and composite bead were investigated. The microbial growth rate decreased with increasing average inlet concentration and increased with increasing operation temperature at average inlet concentration ranging from 50 to 300 ppm and operation temperature ranging from...

journal_title：Applied microbiology and biotechnology

authors： Chan WC,Chang LY

更新日期：2006-08-01 00:00:00

abstract：:The production of the anticoagulant drug heparin from non-animal sources has a number of advantages over the current commercial production of heparin. These advantages include better source material availability, improved quality control, and reduced concerns about animal virus or prion impurities. A bioengineered hep...

journal_title：Applied microbiology and biotechnology

authors： Wang Z,Yang B,Zhang Z,Ly M,Takieddin M,Mousa S,Liu J,Dordick JS,Linhardt RJ

更新日期：2011-07-01 00:00:00

abstract：:A gene encoding beta-glucanase activity from Bacillus amyloliquefaciens was subcloned in both orientations into plasmid shuttle vector pSA3. In only one orientation could a co-integrate be generated with the conjugative plasmid pVA797. The plasmid co-integrate was conjugated into Lactobacillus helveticus strain CNRZ45...

journal_title：Applied microbiology and biotechnology

authors： Thompson K,Collins M

更新日期：1991-06-01 00:00:00

abstract：:Ralstonia eutropha H850 was labelled chromosomally with a gfp marker gene encoding for the green fluorescent protein, and designated R. eutropha H850g13. Visual observation of green fluorescent cells under an epifluorescence microscope, and PCR amplification products, confirmed that the bacterium was labelled with gfp...

journal_title：Applied microbiology and biotechnology

authors： Abbey AM,Beaudette LA,Lee H,Trevors JT

更新日期：2003-12-01 00:00:00

abstract：:The sustainable and practical degradation of intact chicken feathers by a newly isolated thermophilic bacterium Meiothermus ruber H328 was presented with extensive data. Aerobic cultivation with moderately thermophilic strain H328 at 55 degrees C for 6 days led to the apparently complete decay of the truly intact feat...

journal_title：Applied microbiology and biotechnology

authors： Matsui T,Yamada Y,Mitsuya H,Shigeri Y,Yoshida Y,Saito Y,Matsui H,Watanabe K

更新日期：2009-04-01 00:00:00

abstract：:Arthrobacter sp. strain KI72 grows on a 6-aminohexanoate oligomer, which is a by-product of nylon-6 manufacturing, as a sole source of carbon and nitrogen. We cloned the two genes, nylD 1 and nylE 1 , responsible for 6-aminohexanoate metabolism on the basis of the draft genomic DNA sequence of strain KI72. We amplifie...

journal_title：Applied microbiology and biotechnology

authors： Takehara I,Fujii T,Tanimoto Y,Kato DI,Takeo M,Negoro S

更新日期：2018-01-01 00:00:00

abstract：:The thermostable beta-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding p-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyrano...

journal_title：Applied microbiology and biotechnology

authors： Fourage L,Colas B

更新日期：2001-08-01 00:00:00

abstract：:Demand for novel antifungal drugs for medical and agricultural uses has been increasing because of the diversity of pathogenic fungi and the emergence of drug-resistant strains. Genomic resources for various living species, including pathogenic fungi, can be utilized to develop novel and effective antifungal compounds...

journal_title：Applied microbiology and biotechnology

authors： Marui J,Yoshimi A,Hagiwara D,Fujii-Watanabe Y,Oda K,Koike H,Tamano K,Ishii T,Sano M,Machida M,Abe K

更新日期：2010-08-01 00:00:00