Expression and secretion of functional miniantibodies McPC603scFvDhlx in cell-wall-less L-form strains of Proteus mirabilis and Escherichia coli: a comparison of the synthesis capacities of L-form strains with an E. coli producer strain.

abstract：:The present study aimed to evaluate the in vitro antioxidant activities and the protective effect of Rhodobacter sphaeroides on H2O2-induced oxidative stress in Caco-2 cells. The results showed that the antioxidant action of R. sphaeroides varied with different cell concentrations and treatments. Also, the intact cell...

journal_title：Applied microbiology and biotechnology

authors： An J,Yang C,Li Z,Finn PW,Perkins DL,Sun J,Bai Z,Gao L,Zhang M,Ren D

更新日期：2019-01-01 00:00:00

abstract：:A purification procedure for an extracellular alpha-amylase from Streptomyces rimosus, oxytetracycline-producing strain, is described. The enzyme obtained was shown to be an acidic (pI 4.75) monomer with a relative molecular mass (M(r)) of 43,000, containing three cysteines involved in the catalytic activity of the en...

journal_title：Applied microbiology and biotechnology

authors： Vukelić B,Ritonja A,Renko M,Pokorny M,Vitale L

更新日期：1992-05-01 00:00:00

abstract：:The gene encoding NADP(+)-dependent L-1-amino-2-propanol dehydrogenase (AADH) of Rhodococcus erythropolis MAK154 was cloned and sequenced. A 780-bp nucleotide fragment was confirmed to be the gene encoding AADH by agreement of the N-terminal and internal amino acid sequences of the purified AADH. The gene (aadh) codes...

journal_title：Applied microbiology and biotechnology

authors： Kataoka M,Ishige T,Urano N,Nakamura Y,Sakuradani E,Fukui S,Kita S,Sakamoto K,Shimizu S

更新日期：2008-09-01 00:00:00

abstract：:Bacillus cereus is widely distributed food-borne pathogenic bacterium. Due to the harmness to human hearth and the generation of multidrug-resistant B. cereus, it is urgent to develop novel antimicrobial agents. Phage and phage endolysin were taken as novel antimicrobial substance for their specific lytic activity aga...

journal_title：Applied microbiology and biotechnology

authors： Peng Q,Yuan Y

更新日期：2018-09-01 00:00:00

abstract：:Pichia pastoris is widely used as a host system for heterologous protein expression in both academia and industry. Production is typically accomplished by a fed-batch induction process that is known to have negative impacts on cell physiology that impose limits on both protein yields and quality. We have analysed reco...

journal_title：Applied microbiology and biotechnology

authors： Hesketh AR,Castrillo JI,Sawyer T,Archer DB,Oliver SG

更新日期：2013-11-01 00:00:00

abstract：:The primary explosive found in most land mines, 2,4,6-trinitrotoluene (2,4,6-TNT), is often accompanied by 2,4-dinitrotoluene (2,4-DNT) and 1,3-dinitrobenzene (1,3-DNB) impurities. The latter two compounds, being more volatile, have been reported to slowly leak through land mine covers and permeate the soil under whic...

journal_title：Applied microbiology and biotechnology

authors： Yagur-Kroll S,Lalush C,Rosen R,Bachar N,Moskovitz Y,Belkin S

更新日期：2014-01-01 00:00:00

abstract：:Uniform stable-isotope labeling of mammalian cells is achieved via a novel formulation of a serum-free cell culture medium that is based on stable-isotope-labeled autolysates and lipid extracts of various microbiological origin. Yeast autolysates allow complete replacement of individual amino acids and organic acids i...

journal_title：Applied microbiology and biotechnology

authors： Egorova-Zachernyuk TA,Bosman GJ,Degrip WJ

更新日期：2011-01-01 00:00:00

abstract：:The influence of low temperature (5-29 degrees C) on the methanogenic activity of non-adapted digested sewage sludge and on temperature/leachate-adapted biomass was assayed by using municipal landfill leachate, intermediates of anaerobic degradation (propionate) and methane precursors (acetate, H2/CO2) as substrates. ...

journal_title：Applied microbiology and biotechnology

authors： Kettunen RH,Rintala JA

更新日期：1997-10-01 00:00:00

abstract：:Municipal wastewater treatment plants are recognized reservoirs of antibiotic-resistant bacteria. Three municipal wastewater treatment plants differing on the dimensions and bio-treatment processes were compared for the loads of amoxicillin-, tetracycline-, and ciprofloxacin-resistant heterotrophic bacteria, enterobac...

journal_title：Applied microbiology and biotechnology

authors： Novo A,Manaia CM

更新日期：2010-07-01 00:00:00

abstract：:Under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. The phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. The additi...

journal_title：Applied microbiology and biotechnology

authors： Hollender J,Dreyer U,Kornberger L,Kämpfer P,Dott W

更新日期：2002-01-01 00:00:00

abstract：:The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 degrees C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability de...

journal_title：Applied microbiology and biotechnology

authors： Beney L,Marechal PA,Gervais P

更新日期：2001-08-01 00:00:00

abstract：:Microbially induced CaCO3 precipitation (MICP) can give concrete self-healing properties. MICP agents are typically bacterial endospores which are coated into shelled granules, infused into expanded clay, or embedded into superabsorbent polymer (SAP). When small cracks appear in the cured concrete, the encapsulation i...

journal_title：Applied microbiology and biotechnology

authors： Nielsen SD,Koren K,Löbmann K,Hinge M,Scoma A,Kjeldsen KU,Røy H

更新日期：2020-01-01 00:00:00

abstract：:Sulfadiazine (SDZ) is an antibiotic frequently administered to livestock, and it alters microbial communities when entering soils with animal manure, but understanding the interactions of these effects to the prevailing climatic regime has eluded researchers. A climatic factor that strongly controls microbial activity...

journal_title：Applied microbiology and biotechnology

authors： Reichel R,Radl V,Rosendahl I,Albert A,Amelung W,Schloter M,Thiele-Bruhn S

更新日期：2014-01-01 00:00:00

abstract：:Trichothecenes are toxic secondary metabolites produced by filamentous fungi mainly belonging to the Fusarium genus. Production of these mycotoxins occurs during infection of crops and is a threat to human and animal health. Although the pathway for biosynthesis of trichothecenes is well established, the regulation of...

journal_title：Applied microbiology and biotechnology

authors： Merhej J,Richard-Forget F,Barreau C

更新日期：2011-08-01 00:00:00

abstract：:Many hydrocarbon-contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, high salt concentrations, or high pressure, Hydrocarbon-degrading microorganisms, adapted to grow and thrive in these environments, play an important role in the biological treatment of polluted extreme...

journal_title：Applied microbiology and biotechnology

authors： Margesin R,Schinner F

更新日期：2001-09-01 00:00:00

abstract：:The phenomenon of the cell density effect is not readily explained by an obvious nutrient limitation, and a recent study has suggested that for recombinant Autographa californica multiple nucleopolyhedrovirus (rAcMNPV)-infected Sf9 cells, a drop in messenger RNA (mRNA) levels may be sufficient to explain the cell dens...

journal_title：Applied microbiology and biotechnology

authors： Huynh HT,Tran TT,Chan LC,Nielsen LK,Reid S

更新日期：2015-02-01 00:00:00

abstract：:The synergistic effect between polyoxometalates (POMs), namely K(5)[SiW(11)V(V)O(40)]·11H(2)O and H(5)[PMo(10)V(V) (2)O(40)]·13H(2)O and laccase from ascomycete Myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. Such a laccase-mediator system allowed the formation ...

journal_title：Applied microbiology and biotechnology

authors： Kim S,Silva C,Evtuguin DV,Gamelas JA,Cavaco-Paulo A

更新日期：2011-02-01 00:00:00

abstract：:Metarhizium robertsii is a fungus with two lifestyles; it is a plant root symbiont and an insect pathogen. A spontaneously phenotypically degenerated strain of M. robertsii strain ARSEF 2575 (M. robertsii lc-2575; lc = low conidiation) showed a reduction in conidiation and fungal virulence after successive subculturin...

journal_title：Applied microbiology and biotechnology

authors： Hu S,Bidochka MJ

更新日期：2020-06-01 00:00:00

abstract：:Biofilms of selected bacteria strains were previously used on metal coupons as a protective layer against microbiologically influenced corrosion of metals. Unlike metal surfaces, concrete surfaces present a hostile environment for growing a protective biofilm. The main objective of this research was to investigate whe...

journal_title：Applied microbiology and biotechnology

authors： Soleimani S,Ormeci B,Isgor OB

更新日期：2013-02-01 00:00:00

abstract：:Acrylic polymers (AP) are a diverse group of materials with broad applications, frequent use, and increasing demand. Some of the most used AP are polyacrylamide, polyacrylic acid, polymethyl methacrylates, and polyacrylonitrile. Although no information for the production of all AP types is published, data for the most...

journal_title：Applied microbiology and biotechnology

authors： Gaytán I,Burelo M,Loza-Tavera H

更新日期：2021-01-11 00:00:00

abstract：:Histone lysine acetylation orchestrates transcriptional activity essential for diverse cellular events across organisms, but it remains poorly understood how an acetylated lysine affects cellular functions in filamentous fungal pathogens. Here, we show the functions of a histone acetyltransferase that is phylogenetica...

journal_title：Applied microbiology and biotechnology

authors： Wang JJ,Cai Q,Qiu L,Ying SH,Feng MG

更新日期：2018-02-01 00:00:00

abstract：:Photosynthetic microorganisms produce relatively large amounts of physiologically active materials which stimulate the physiological activity of other organisms. In this study, mammalian HeLa cells were cultured in different culture media which were Dulbecco's modified Eagle medium (DMEM) with newborn calf serum (NCS)...

journal_title：Applied microbiology and biotechnology

authors： Lee HJ,Park JY,Yoo KS,Yoon J,Kim YH,Min J

更新日期：2013-11-01 00:00:00

abstract：:Glucose 6-phosphate dehydrogenase (G6PDH) is one of the most important dehydrogenases responsible for generating reduced NADPH for anabolism and is also the rate-limiting enzyme in the Entner-Doudoroff pathway. For in vitro biocatalysis, G6PDH must possess both high activity and good thermostability due to requirement...

journal_title：Applied microbiology and biotechnology

authors： Huang R,Chen H,Zhou W,Ma C,Zhang YP

更新日期：2018-04-01 00:00:00

abstract：:Rhizopus oryzae is a filamentous fungus belonging to the Zygomycetes. It is among others known for its ability to produce the sustainable platform chemicals L: -(+)-lactic acid, fumaric acid, and ethanol. During glycolysis, all fermentable carbon sources are metabolized to pyruvate and subsequently distributed over th...

journal_title：Applied microbiology and biotechnology

authors： Meussen BJ,de Graaff LH,Sanders JP,Weusthuis RA

更新日期：2012-05-01 00:00:00

abstract：:Two-dimensional gel electrophoresis was conducted to investigate the effect of H2O2 on whole protein expression in Acinetobacter oleivorans DR1. Functional classification of 13 upregulated proteins using MALDI-TOF mass spectrometry showed relationships with oxidative stress, energy production and conversion, nucleotid...

journal_title：Applied microbiology and biotechnology

authors： Kim J,Cho Y,Jang IA,Park W

更新日期：2015-12-01 00:00:00

abstract：:Escherichia coli and Lactobacillus plantarum were subjected to final water potentials of -5.6 MPa and -11.5 MPa with three solutes: glycerol, sorbitol and NaCl. The water potential decrease was realized either rapidly (osmotic shock) or slowly (20 min) and a difference in cell viability between these conditions was on...

journal_title：Applied microbiology and biotechnology

authors： Poirier I,Maréchal PA,Evrard C,Gervais P

更新日期：1998-12-01 00:00:00

abstract：:Non-covalent interaction of alcohol dehydrogenase with polysaccharides was studied using three neutral and three anionic polysaccharides. The process of interaction of alcohol dehydrogenase with gum Arabic was optimized with respect to the ratio of enzyme to gum Arabic, pH, and molarity of buffer. Alcohol dehydrogenas...

journal_title：Applied microbiology and biotechnology

authors： Jadhav SB,Bankar SB,Granström T,Ojamo H,Singhal RS,Survase SA

更新日期：2014-01-01 00:00:00

abstract：:The knowledge of biosynthesis gene clusters, production improving methods, and bioactivity mechanisms is very important for the development of filamentous fungi metabolites. Metabolic engineering and heterologous expression methods can be applied to improve desired metabolite production, when their biosynthesis pathwa...

journal_title：Applied microbiology and biotechnology

authors： Yin Y,Cai M,Zhou X,Li Z,Zhang Y

更新日期：2016-09-01 00:00:00

abstract：:Significant resources are invested into efforts to improve the production yields of natural products from Actinobacteria, a well-recognized source of leads for several industries, most notably pharmaceutical one. Introduction of changes into genes for ribosomal protein S12 (rpsL) and/or 16S rRNA methylation (rsmG) is ...

journal_title：Applied microbiology and biotechnology

authors： Lopatniuk M,Myronovskyi M,Nottebrock A,Busche T,Kalinowski J,Ostash B,Fedorenko V,Luzhetskyy A

更新日期：2019-09-01 00:00:00

abstract：:Cytidine diphosphate choline (CDP-choline) has been applied for treating acute craniocerebral injury and allowing recovery of consciousness after brain surgery. In this study, an acetate kinase (ACK)/acetyl phosphate system was used to supply ATP and combined with Escherichia coli-overexpressed CMP kinase (CMK), NDP k...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Wang J,Xu C,Chen Y,Yang J,Liu D,Niu H,Jiang Y,Yang S,Ying H

更新日期：2017-02-01 00:00:00