Influence of stringent and relaxed response on excretion of recombinant proteins and fatty acid composition in Escherichia coli.

Abstract:

:In contrast to stringent (relA+) cells of Escherichia coli, relaxed (relA) cells excreted recombinant proteins (beta-lactamase, interferon alpha 1) into the culture medium during amino acid limitation. Comparative analyses of overall fatty acid composition in relA+ cells and relA cells were performed and revealed that, in wild-type cells, drastic alterations occurred during the stringent response. The portion of saturated fatty acids (C16:0) and the fractions of cyclopropane fatty acids (C17cyc and C19cyc) increased whereas the portions of unsaturated fatty acids (C16:1 and C18:1) decreased. In cells of the relaxed mutant, no significant changes in the overall composition of the fatty acids were observed after the onset of amino acid limitation. These results indicate that a change in fatty acid composition of membrane lipids after starvation of cells may be responsible for the prevention of loss of cellular proteins into the culture medium in stringent controlled cells of Escherichia coli.

authors

Gitter B,Diefenbach R,Keweloh H,Riesenberg D

doi

10.1007/BF00170628

subject

Has Abstract

pub_date

1995-04-01 00:00:00

pages

89-92

issue

1

eissn

0175-7598

issn

1432-0614

journal_volume

43

pub_type

杂志文章
  • Expression of membrane-bound dehydrogenases from a mother of vinegar metagenome in Gluconobacter oxydans.

    abstract::Acetic acid bacteria are well-known for their membrane-bound dehydrogenases rapidly oxidizing a variety of substrates in the periplasm. Since many acetic acid bacteria have not been successfully cultured in the laboratory yet, studying membrane-bound dehydrogenases directly from a metagenome of vinegar microbiota seem...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8479-y

    authors: Peters B,Mientus M,Kostner D,Daniel R,Liebl W,Ehrenreich A

    更新日期:2017-11-01 00:00:00

  • Economic considerations in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by bacterial fermentation.

    abstract::The process for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)] by bacterial fermentation and its recovery was analysed. The effects of various factors such as P(3HB/V) content, P(3HB/V) productivity, P(3HB/V) yield and 3-hydroxyvalerate (3HV) fraction in P(3HB/V) on the production cost of P(...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530000326

    authors: Choi J,Lee SY

    更新日期:2000-06-01 00:00:00

  • Current advances in Phellinus sensu lato: medicinal species, functions, metabolites and mechanisms.

    abstract::Twenty-six species of Phellinus sensu lato, reported as medicinal mushrooms, are enumerated in this review. The species' names were checked and revised according to contemporary taxonomy and the latest version of the International Code of Botanical Nomenclature (Vienna Code); two misapplied names of Phellinus baumii P...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-010-2711-3

    authors: Dai YC,Zhou LW,Cui BK,Chen YQ,Decock C

    更新日期:2010-08-01 00:00:00

  • Genetically engineered microbial biosensors for in situ monitoring of environmental pollution.

    abstract::Microbial biosensors are compact, portable, cost effective, and simple to use, making them seem eminently suitable for the in situ monitoring of environmental pollution. One promising approach for such applications is the fusion of reporter genes with regulatory genes that are dose-dependently responsive to the target...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-010-2990-8

    authors: Shin HJ

    更新日期:2011-02-01 00:00:00

  • Asparaginase inhibition of adhesion of type 1-fimbriated and P-fimbriated Escherichia coli to epithelial cell receptors.

    abstract::The 3D structures of Fim H and PapG proteins complexed with the host carbohydrate receptor demonstrate that both utilize binding-pocket asparagines for contact or stabilization with the carbohydrate. Pretreatment of whole bacteria with asparaginase resulted in decreased fimbriae-mediated attachment to urinary epitheli...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-004-1710-7

    authors: Wehner MR,Koch SR,Kindinger L,Nelson N,Cowan MM

    更新日期:2004-11-01 00:00:00

  • Rhamnolipid production: effect of oxidative stress on virulence factors and proteome of Pseudomonas aeruginosa PA1.

    abstract::Under specific environmental conditions, Pseudomonas aeruginosa produces a biodegradable surfactant rhamnolipid. Evidences suggest that this biosurfactant is involved in protecting cells against oxidative stress; however, the effects of oxidative stress on its production and other virulence factors are still unclear. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-4258-y

    authors: Pacheco GJ,Reis RS,Fernandes AC,da Rocha SL,Pereira MD,Perales J,Freire DM

    更新日期:2012-09-01 00:00:00

  • Inhibitory effect of hydroxycinnamic acids on Dekkera spp.

    abstract::Simple phenolic components of wine, hydroxycinnamic acids (HCAs) are known to have antimicrobial properties. This study sought to determine the potential of ferulic acid as an antifungal agent for the control of Dekkera. Growth was inhibited by all HCAs examined in this study, with ferulic acid being the most potent a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-009-2352-6

    authors: Harris V,Jiranek V,Ford CM,Grbin PR

    更新日期:2010-03-01 00:00:00

  • Cloning, sequencing, overexpression and characterization of L-rhamnose isomerase from Bacillus pallidus Y25 for rare sugar production.

    abstract::The L-rhamnose isomerase gene (L-rhi) encoding for L-rhamnose isomerase (L-RhI) from Bacillus pallidus Y25, a facultative thermophilic bacterium, was cloned and overexpressed in Escherichia coli with a cooperation of the 6xHis sequence at a C-terminal of the protein. The open reading frame of L-rhi consisted of 1,236 ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-1109-3

    authors: Poonperm W,Takata G,Okada H,Morimoto K,Granström TB,Izumori K

    更新日期:2007-10-01 00:00:00

  • Heat preadaptation improved the ability of Zygosaccharomyces rouxii to salt stress: a combined physiological and transcriptomic analysis.

    abstract::Zygosaccharomyces rouxii plays important roles in the brewing process of fermented foods such as soy sauce, where salt stress is a frequently encountered condition. In this study, effect of heat preadaptation on salt tolerance of Z. rouxii and the protective mechanisms underlying heat preadaptation were investigated b...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-11005-z

    authors: Wang D,Zhang M,Huang J,Zhou R,Jin Y,Zhao D,Zheng J,Wu C

    更新日期:2021-01-01 00:00:00

  • The microbial coinfection in COVID-19.

    abstract::The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel β-coronavirus, is the main pathogenic agent of the rapidly spreading pneumonia called coronavirus disease 2019 (COVID-19). SARS-CoV-2 infects much more people, especially the elder population, around the world than other coronavirus, such as SAR...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-020-10814-6

    authors: Chen X,Liao B,Cheng L,Peng X,Xu X,Li Y,Hu T,Li J,Zhou X,Ren B

    更新日期:2020-09-01 00:00:00

  • Mitogenome of Tolypocladium guangdongense.

    abstract::Tolypocladium guangdongense is a high-value edible fungus with various medicinal and food safety properties. However, its evolutionary and genetic information is still limited. Mitochondrial genomes are potential models for molecular evolution and phylogenetic studies. In this study, we sequenced the complete mitogeno...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10889-1

    authors: Zhang C,Dai Y,Wang G,Wang C,Gao Y,Deng W,Li T

    更新日期:2020-11-01 00:00:00

  • Tafuketide, a phylogeny-guided discovery of a new polyketide from Talaromyces funiculosus Salicorn 58.

    abstract::A phylogeny-guided approach was applied to screen endophytic fungi containing type I polyketide synthase (PKS I) biosynthetic gene sequences and aimed to correlate genotype to chemotype for the discovery of novel bioactive polyketides. Salicorn 58, which was identified as Talaromyces funiculosus based on its internal ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7311-4

    authors: Guo J,Ran H,Zeng J,Liu D,Xin Z

    更新日期:2016-06-01 00:00:00

  • Degradation of lindane by a novel embedded bio-nano hybrid system in aqueous environment.

    abstract::The objective of this study was to evaluate the effect of an embedded bio-nano hybrid system using nanoscale zinc oxide (n-ZnO) and lindane-degrading yeast Candida VITJzN04 for lindane degradation. Nano-embedding of the yeast was done with chemically synthesized n-ZnO particles (50 mg/mL) and was visualized by atomic ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-6112-x

    authors: Salam JA,Das N

    更新日期:2015-03-01 00:00:00

  • Mining Xanthomonas and Streptomyces genomes for new pectinase-encoding sequences and their heterologous expression in Escherichia coli.

    abstract::Microbial genome sequencing has left a legacy of annotated yet uncharacterized genes or open reading frames, activities that may have useful applications in health and/or the environment. We are interested in the discovery and characterization of potentially new pectinolytic activities for the enzymatic retting of nat...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-008-1389-2

    authors: Xiao Z,Boyd J,Grosse S,Beauchemin M,Coupe E,Lau PC

    更新日期:2008-04-01 00:00:00

  • Metaproteomic strategies and applications for gut microbial research.

    abstract::The human intestine hosts various complex microbial communities that are closely associated with multiple health and disease processes. Determining the composition and function of these microbial communities is critical to unveil disease mechanisms and promote human health. Recently, meta-omic strategies have been dev...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-017-8215-7

    authors: Xiao M,Yang J,Feng Y,Zhu Y,Chai X,Wang Y

    更新日期:2017-04-01 00:00:00

  • Extracellular production of lipoxygenase from Anabaena sp. PCC 7120 in Bacillus subtilis and its effect on wheat protein.

    abstract::In this study, the lipoxygenase (ana-LOX) gene from Anabaena sp. PCC 7120 was successful expressed and secreted in Bacillus subtilis. Under the control of the P43 promoter, with a signal peptide from the B. subtilis 168 nprB gene, and facilitated by the molecular chaperone PrsA, the production of the recombinant ana-L...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-3895-5

    authors: Zhang C,Tao T,Ying Q,Zhang D,Lu F,Bie X,Lu Z

    更新日期:2012-05-01 00:00:00

  • Selective enrichment and characterization of a phosphorus-removing bacterial consortium from activated sludge.

    abstract::Under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. The phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. The additi...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-001-0847-x

    authors: Hollender J,Dreyer U,Kornberger L,Kämpfer P,Dott W

    更新日期:2002-01-01 00:00:00

  • Behavioral response of dissimilatory perchlorate-reducing bacteria to different electron acceptors.

    abstract::The response behavior of three dissimilatory perchlorate-reducing bacteria to different electron acceptors (nitrate, chlorate, and perchlorate) was investigated with two different assays. The observed response was species-specific, dependent on the prior growth conditions, and was inhibited by oxygen. We observed attr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-009-2051-3

    authors: Sun Y,Gustavson RL,Ali N,Weber KA,Westphal LL,Coates JD

    更新日期:2009-10-01 00:00:00

  • Amplicon-based profiling of bacteria in raw and secondary treated wastewater from treatment plants across Australia.

    abstract::In this study, we investigated the use of Illumina high-throughput sequencing of 16S ribosomal RNA (rRNA) amplicons to explore microbial diversity and community structure in raw and secondary treated wastewater (WW) samples from four municipal wastewater treatment plants (WWTPs A-D) across Australia. Sequence reads we...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7959-9

    authors: Ahmed W,Staley C,Sidhu J,Sadowsky M,Toze S

    更新日期:2017-02-01 00:00:00

  • Small P particles formed by the Taiwan-native norovirus P domain overexpressed in Komagataella pastoris.

    abstract::The protrusion (P) domain of the major structural protein VP1 of norovirus (NoV) is critical for the host's immune response and receptor binding. Most heterologous P domains expressed in Escherichia coli or Komagataella pastoris (formally known as Pichia pastoris) form P particles consisting of 24 P monomers formed th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9331-8

    authors: Chen YL,Chang PJ,Huang CT

    更新日期:2018-11-01 00:00:00

  • Establishment of a hyper-protein production system in submerged Aspergillus oryzae culture under tyrosinase-encoding gene (melO) promoter control.

    abstract::UV-mediated mutagenesis generated a high glucoamylase-producing mutant of Aspergillus oryzae exhibiting strong melanization in solid-state culture. Expression of the glucoamylase-encoding gene (glaB), which is specifically expressed in solid-state culture, and the tyrosinase-encoding gene (melO), was analyzed using an...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530100771

    authors: Ishida H,Matsumura K,Hata Y,Kawato A,Suginami K,Abe Y,Imayasu S,Ichishima E

    更新日期:2001-10-01 00:00:00

  • Characterization of a novel phage infecting the pathogenic multidrug-resistant Bacillus cereus and functional analysis of its endolysin.

    abstract::Bacillus cereus is widely distributed food-borne pathogenic bacterium. Due to the harmness to human hearth and the generation of multidrug-resistant B. cereus, it is urgent to develop novel antimicrobial agents. Phage and phage endolysin were taken as novel antimicrobial substance for their specific lytic activity aga...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9219-7

    authors: Peng Q,Yuan Y

    更新日期:2018-09-01 00:00:00

  • Proteome signatures--how are they obtained and what do they teach us?

    abstract::The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of bio...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-015-6795-7

    authors: da Costa JP,Carvalhais V,Ferreira R,Amado F,Vilanova M,Cerca N,Vitorino R

    更新日期:2015-09-01 00:00:00

  • Overexpression of the gene encoding HMG-CoA reductase in Saccharomyces cerevisiae for production of prenyl alcohols.

    abstract::To develop microbial production method for prenyl alcohols (e.g., (E,E)-farnesol (FOH), (E)-nerolidol (NOH), and (E,E,E)-geranylgeraniol (GGOH)), the genes encoding enzymes in the mevalonate and prenyl diphosphate pathways were overexpressed in Saccharomyces cerevisiae, and the resultant transformants were evaluated a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-008-1807-5

    authors: Ohto C,Muramatsu M,Obata S,Sakuradani E,Shimizu S

    更新日期:2009-04-01 00:00:00

  • Conversion of phenol to glutamate and proline in Corynebacterium glutamicum is regulated by transcriptional regulator ArgR.

    abstract::This paper reports a novel integrated metabolic pathway of amino acid production through the utilization of phenol in Corynebacterium glutamicum. In the presence of 8.5 mM phenol, the level of glutamate and proline production increased up to 1.2- and 14.7-fold, respectively, compared to the control condition. In addit...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-009-2206-2

    authors: Lee SY,Kim YH,Min J

    更新日期:2010-01-01 00:00:00

  • The histone acetyltransferase Mst2 sustains the biological control potential of a fungal insect pathogen through transcriptional regulation.

    abstract::Histone lysine acetylation orchestrates transcriptional activity essential for diverse cellular events across organisms, but it remains poorly understood how an acetylated lysine affects cellular functions in filamentous fungal pathogens. Here, we show the functions of a histone acetyltransferase that is phylogenetica...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8703-9

    authors: Wang JJ,Cai Q,Qiu L,Ying SH,Feng MG

    更新日期:2018-02-01 00:00:00

  • Microorganism-immobilized carbon nanoparticle anode for microbial fuel cells based on direct electron transfer.

    abstract::A fast and convenient bacterial immobilization method was proposed as an attempt to improve the anode efficiency of a microbial fuel cell, in which bacteria were entrapped into carbon nanoparticle matrix. The direct electron transfer from the entrapped bacterial cells to the anode was verified using cyclic voltammogra...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-010-3013-5

    authors: Yuan Y,Zhou S,Xu N,Zhuang L

    更新日期:2011-03-01 00:00:00

  • 2,3-Butanediol production from cellobiose using exogenous beta-glucosidase-expressing Bacillus subtilis.

    abstract::We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7326-x

    authors: Tanimura K,Takashima S,Matsumoto T,Tanaka T,Kondo A

    更新日期:2016-07-01 00:00:00

  • Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities.

    abstract::Thirty-four strains of lactic acid bacteria (seven Bifidobacterium, 11 Lactobacillus, six Lactococcus, and 10 Streptococcus thermophilus) were assayed in vitro for antioxidant activity against ascorbic and linolenic acid oxidation (TAA(AA) and TAA(LA)), trolox-equivalent antioxidant capacity (TEAC), intracellular glut...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-4241-7

    authors: Amaretti A,di Nunzio M,Pompei A,Raimondi S,Rossi M,Bordoni A

    更新日期:2013-01-01 00:00:00

  • A novel heterotrophic nitrifying and aerobic denitrifying bacterium, Zobellella taiwanensis DN-7, can remove high-strength ammonium.

    abstract::A novel heterotrophic bacterium capable of heterotrophic nitrification and aerobic denitrification was isolated from ammonium contaminated landfill leachate and physiochemical and phylogenetically identified as Zobellella taiwanensis DN-7. DN-7 converted nitrate, nitrate, and ammonium to N2 as the primary end product....

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7290-5

    authors: Lei Y,Wang Y,Liu H,Xi C,Song L

    更新日期:2016-05-01 00:00:00